cwrs advisory board meeting march 1, 2007 gretchen grover – chemist christal wade - microbiologist...
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![Page 1: CWRS Advisory Board Meeting March 1, 2007 Gretchen Grover – Chemist Christal Wade - Microbiologist Rick Fowler – Environmental Biotechnologist](https://reader035.vdocument.in/reader035/viewer/2022062216/56649d975503460f94a80a81/html5/thumbnails/1.jpg)
CWRS Advisory Board MeetingCWRS Advisory Board MeetingMarch 1, 2007March 1, 2007
Gretchen Grover – ChemistGretchen Grover – ChemistChristal Wade - MicrobiologistChristal Wade - Microbiologist
Rick Fowler – Environmental BiotechnologistRick Fowler – Environmental Biotechnologist
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WATERS EmployeesWATERS Employees Director: Director: Jana FatticJana Fattic Lab Analysts:Lab Analysts:
Microbiology Department: Rick Fowler, Christal Microbiology Department: Rick Fowler, Christal WadeWade
Chemistry Department: Gretchen Grover, Chemistry Department: Gretchen Grover, Dong LiDong Li
Office Associate: Office Associate: Kristy MayesKristy Mayes Students: Students: Samantha Kramer, Rebekkah Lively, Samantha Kramer, Rebekkah Lively,
Jennifer DeanJennifer Dean
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CustomersCustomers Private
Total Coliform/E. Coli, Metals, Ions, AtrazineTotal Coliform/E. Coli, Metals, Ions, Atrazine Wastewater
BOD, pH, TRC, DO, TSS, Fecal Coliform, Ammonia BOD, pH, TRC, DO, TSS, Fecal Coliform, Ammonia NitrogenNitrogen
Storm water COD, Oil and Grease, TDS, TSSCOD, Oil and Grease, TDS, TSS
Drinking water Total Coliform/E.ColiTotal Coliform/E.Coli
Oil Wells pH, TDS, Specific Gravity, Chloride pH, TDS, Specific Gravity, Chloride
Research Projects TOC, Atrazine, Metals, Ions, DNATOC, Atrazine, Metals, Ions, DNA
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CertificationsCertifications Drinking water CertificationDrinking water Certification
January AuditJanuary Audit Inorganic CertificationInorganic Certification
June AuditJune Audit Cryptosporidium Cryptosporidium
Long Term 2 Enhanced Long Term 2 Enhanced Surface Water Treatment Surface Water Treatment Rule (LT2)Rule (LT2)
Accept outside samples under Accept outside samples under LT2 and provides field LT2 and provides field filtration apparatusfiltration apparatus
www.epa.gov/safewater/disinfection/www.epa.gov/safewater/disinfection/LT2/lab_aprvlabs.htmlLT2/lab_aprvlabs.html
Chemical Hygiene PlanChemical Hygiene Plan Chemical Hygiene OfficerChemical Hygiene Officer
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ProjectsProjects Mammoth CaveMammoth Cave
Flood Pulse EventFlood Pulse Event City of Bowling GreenCity of Bowling Green
River & Cave System monitoringRiver & Cave System monitoring Watershed WatchWatershed Watch
Upper Green River watershed monitoring (E.coli)Upper Green River watershed monitoring (E.coli) TMDLTMDL
Analyzing samples for this projectAnalyzing samples for this project University ResearchUniversity Research
Support WKU faculty as well as other university Support WKU faculty as well as other university projectsprojects
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Quality AssuranceQuality Assurance Continued revision and updating of Continued revision and updating of
SOP to the 21SOP to the 21stst Edition Standard Edition Standard Methods Methods
QA/QC ImprovementsQA/QC Improvements New StandardsNew Standards
PT/QC (quarterly)PT/QC (quarterly) USGSUSGS APGAPG
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Important Important PurchasesPurchases
ChemistryChemistry Accumet excel XL60Accumet excel XL60
TKN DigestorTKN Digestor KonelabKonelab
MicrobiologyMicrobiology Bench top water bath
FastGel electrophoresis apparatus PCR Workstation
Qubit fluorometer Microcentrifuge
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Where do we go from here?Where do we go from here? Current CapabilitiesCurrent Capabilities
CryptosporidiumCryptosporidium Konelab AnalysesKonelab Analyses Drinking water, Wastewater, Storm water analysesDrinking water, Wastewater, Storm water analyses Bacteriological/DNA analysesBacteriological/DNA analyses
Future CapabilitiesFuture Capabilities Unregulated Contaminant Monitoring Rule (UCMR2)Unregulated Contaminant Monitoring Rule (UCMR2) Organics (Work toward a GC MS/MS and HPLC)Organics (Work toward a GC MS/MS and HPLC) RadionuclidesRadionuclides Direct a Chemical Laboratory Certification Program Direct a Chemical Laboratory Certification Program
for Kentuckyfor Kentucky Voluntary Wastewater CertificationVoluntary Wastewater Certification
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Where do we go from here?Where do we go from here?
New customers bring more cash flowNew customers bring more cash flow 11 new customers 11 new customers Networking; customer surveysNetworking; customer surveys Pick up servicePick up service Consultation servicesConsultation services Sample collection servicesSample collection services
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Microbiology Microbiology ProgramsPrograms
Christal WadeChristal Wade
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Drinking Water MicrobiologyDrinking Water Microbiology
Sample Type Analyzed Number Analyzed/year
Drinking Water (Public) 2080
Drinking Water (Private) 25
Wastewater 0
Surface (raw) 745
Total 2850
Laboratory Sample Load
Completed latest On-Site Audit in January 2007
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EPA Approval for CryptoEPA Approval for Crypto WATERS Lab received approval from WATERS Lab received approval from
EPA for the analysis of Cryptosporidium EPA for the analysis of Cryptosporidium on October 18, 2006.on October 18, 2006.
Currently have a cooperative Currently have a cooperative agreement with Appalachian States agreement with Appalachian States Analytical, L.L.C.Analytical, L.L.C. Total of 20 surface water clientsTotal of 20 surface water clients Will be starting monitoring in JuneWill be starting monitoring in June
4 Contracts with other PWSs4 Contracts with other PWSs From both Kentucky and West VirginiaFrom both Kentucky and West Virginia Several quotes outstandingSeveral quotes outstanding
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Latest PT ResultsLatest PT ResultsFrom September 2006From September 2006
Oocysts Reported
Oocysts Spiked
% RecoveredCysts
ReportedCysts
Spiked%
Recovered
03604 152 195 78 100 201 50
03605 139 195 71 91 201 45
03606 138 180 77 0 0 N/A
75 48
5 7RSD of % Recovery
EPA Protozoan Proficiency Testing Laboratory Results Summary
Lab ID: KY00002Round: 0906 Filter: Envirochek HV
Sample ID
Cryptosporidium Giardia
Mean % Recovery
Completed another set of PT samples in February 2007
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Mean (%) Median (%) RSD (%) Mean (%) Median (%) RSD (%)
Envirochek 1 35 35 31 41 41 21
Envirochek HV 45 45 43 42 46 48 27
Filta-Max 15 47 46 35 39 43 44
Filta-Max Xpress 4 29 27 34 29 26 41
Direct Centrifugation
1 61 61 1 49 49 25
Continuous Flow Centrifugation
1 69 68 5 62 62 5
CrypTest 1 59 65 23 5 5 141
All Versions 68 45 44 41 43 46 35
WATERS Lab 75 5 48 7
OPT Recovery Statistics for Each Method Variation
Giardia RecoveriesCryptosprodium RecoveriesMethod Version # Labs
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Cryptosporidium OPR
0.0
0.2
0.4
0.6
0.8
1.006
247-
02
0625
1-02
0626
4-02
0627
8-02
0629
2-02
0630
6-02
0632
0-02
0633
4-02
0634
8-02
0636
2-02
0701
1-02
0702
5-02
0703
8-02
Sample Identification
Per
cen
t R
eco
very
Ongoing Precision and Recovery
Mean Recovery 52.4%
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Giardia OPR
0.00.10.20.30.40.50.60.70.80.91.0
06247-0
2
06251-0
2
06264-0
2
06278-0
2
06292-0
2
06306-0
2
06320-0
2
06334-0
2
06348-0
2
06362-0
2
07011-0
2
07025-0
2
07038-0
2
Sample Identification
Per
cen
t R
eco
very
Ongoing Precision and Recovery
Mean Recovery 41.1%
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Education & OutreachEducation & Outreach 2 Hour Crypto Presentation2 Hour Crypto Presentation
Approved for Drinking Water Operator Approved for Drinking Water Operator CEUCEU
Presented at KRWA Training SessionsPresented at KRWA Training Sessions Will Present at KWWOA Annual Will Present at KWWOA Annual
ConferenceConference 1 Hour 1 Hour E. coliE. coli Presentation Presentation
Approved for Waste Water Operator CEUApproved for Waste Water Operator CEU Presented at KRWA Training SessionsPresented at KRWA Training Sessions Will Present for KWWOAWill Present for KWWOA
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Environmental Environmental BiotechnologyBiotechnology
RickRick FowlerFowler
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DNA Analysis ServicesDNA Analysis Services DNA extraction from water, soil, membrane DNA extraction from water, soil, membrane
filters, pure cultures, swabs, etc.filters, pure cultures, swabs, etc. Yield of total DNA measured with Qubit Yield of total DNA measured with Qubit
fluorometer, sensitivity 0.2 x 10fluorometer, sensitivity 0.2 x 10-12 -12 gg Quantitative Real-Time PCRQuantitative Real-Time PCR
Determines concentration of DNA from specific Determines concentration of DNA from specific organisms in a mixture, sensitivity <1 pptorganisms in a mixture, sensitivity <1 ppt
DNA cloning and sequencingDNA cloning and sequencing Cloning permits segregation of individual Cloning permits segregation of individual
species comprising environmental microbial species comprising environmental microbial communities communities
DNA sequencing yields unique gene sequences DNA sequencing yields unique gene sequences for identification of organismsfor identification of organisms
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Bacteria
Parasites Algae
Fungi Amoebae
Zooplankton
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Limestone
Ca13CO3
Field work in Mammoth Field work in Mammoth CaveCave
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Examples of clients Examples of clients supported by WATERS DNA supported by WATERS DNA
servicesservices Louisville Water CompanyLouisville Water Company Ammonia Oxidizing Bacteria in distribution systemAmmonia Oxidizing Bacteria in distribution system AcanthamoebaAcanthamoeba in eyewash stations in eyewash stations
KSU Aquaculture Research CenterKSU Aquaculture Research Center DNA analysis of bacteria, algae, and fungi used to DNA analysis of bacteria, algae, and fungi used to
evaluate conditions for optimal shrimp productionevaluate conditions for optimal shrimp production Fungi in some tanks identified by cloning and DNA Fungi in some tanks identified by cloning and DNA
sequencing to assess potential risk or benefitsequencing to assess potential risk or benefit National Cave and Karst Research InstituteNational Cave and Karst Research Institute
Develop a universal monitoring scheme applicable to Develop a universal monitoring scheme applicable to caves nationwide and catalog any new caves nationwide and catalog any new microorganisms indigenous to cave climatesmicroorganisms indigenous to cave climates
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Examples, continuedExamples, continued Mammoth Cave National ParkMammoth Cave National Park
Ongoing monitoring of bacterial communities for Ongoing monitoring of bacterial communities for better understanding of karst aquifers and as better understanding of karst aquifers and as indicators of environmental changesindicators of environmental changes
Monitor Monitor Chlorella Chlorella DNA in surface waters to assist DNA in surface waters to assist with protection of endangered mussellswith protection of endangered mussells
Oregon Caves National MonumentOregon Caves National Monument Cloning and sequencing of bacterial and fungal Cloning and sequencing of bacterial and fungal
communities on- and off-tourist trails to assess communities on- and off-tourist trails to assess environmental impacts of tourismenvironmental impacts of tourism
Abundance of iron-sulfur bacteria indicate growth Abundance of iron-sulfur bacteria indicate growth in absence of light is derived from chemical energy in absence of light is derived from chemical energy
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Scientific Outreach, Scientific Outreach, Presentations, and Presentations, and
WorkshopsWorkshops National Speleological SocietyNational Speleological Society
2001, 20062001, 2006 National Cave and Karst Research National Cave and Karst Research
InstituteInstitute 2001, 20052001, 2005
Geological Society of AmericaGeological Society of America 2001, 2004 2001, 2004
National Park Service advisory panelsNational Park Service advisory panels 2001, 2002, 2003, 2004, 2005, 20062001, 2002, 2003, 2004, 2005, 2006
ISEISISEIS 20062006