cytology the structure of the cell at light and …...cytology the structure of the cell at light...
TRANSCRIPT
CYTOLOGY the structure of the CELL at light
and electron-microscopic level
Institute of Histology and EmbryologyFaculty of Medicine
Comenius University
Practical training N.2
SPECIMENS
• DNA - the nuclei of the epithelial cells,
Vagina (IH-E)
• RNA – ribosomes, rough ER, nucleolus,
Spinal cord (HE)
• Mitochondria, Kidney (Regaud)
• Glycogen, Liver of rat (PAS or PAS-H)
• Pigment granules, pigmentary epithelium of
Retina (without staining)
• Brush border, microvilli – Kidney (AP)
Specimen:
DNA - the nuclei of the
epithelial cells,
Vagina
Iron hematoxylin and Eosin
Various types of nuclei in the cells
of stratified squamous epithelium
nonkeratinized
DNA – nuclei of the epithelial cells, Vagina (IH-E)
DNA – nuclei of the epithelila cells, Vagina (IH)
Specimen:
RNA – ribosomes, rough ER,
nucleolus, Spinal cord
hematoxylin and eosin
Nissl substance (tigroid) – rER in neuron
Spinal
cord
Anterior horn
RNA – ribosomes, rough ER, nucleolus, Spinal cord (HE)
Specimen:
Demonstration of mitochondria
in the cells of proximal tubules,
Kidney
Regaud
The cell of proximal tubulein kidney,mitochondria
Mitochondria, kidney (Regaud)
Mitochondria, kidney (Regaud)
erytrocytes
mitochondria
lumen
of tubule
Mitochondria, kidney (Regaud)
Specimen:
Demonstration of glycogen
in hepatocytes, Liver
PAS–H
Glycogen
particules
in hepatocyte
at EM level
Glycogen - Liver (PAS-H)
Glycogen - Liver (PAS-H)
nucleus
Glycogen - Liver (PAS-H)
nuclei
glycogen
Glycogen - Liver (PAS-H)
glycogen
Specimen:
Demonstration of endogenic
pigments –
pigmentary epithelium of Retina,
Eye ball
without any staining
Pigmentary epithelium, retina
Unstained nucleus
Pigment granules
Specimen:
Demonstration of specialized
cellular surfaces: microvilli in the
lining epithelial cells of proximal
tubules, Kidney
Evidence of positivity (red) of alkaline
phosphatase bounded at the surface
of microvilli
Microvilli – kidney (AP)