d8.2 report lamp sensor final - aquacosm · figure 3 shows a general view of the lamp with the...

DeliverableNo8.2:ReportonthecurrentstatusandaimsforLAMPSensorSystem

ProjectTitle: AQUACOSM: NetworkofLeadingEuropeanAQUAticMesoCOSMFacilities

ConnectingMountainstoOceansfromtheArctictotheMediterranean

Projectnumber: 732065

ProjectAcronym: AQUACOSM

Proposalfulltitle: NetworkofLeadingEuropeanAQUAticMesoCOSMFacilities

ConnectingMountainstoOceansfromtheArctictotheMediterranean

Type: Researchandinnovationactions

Workprogramtopicsaddressed:

H2020-INFRAIA-2016-2017:IntegratingandopeningresearchinfrastructuresofEuropeaninterest

Duedateofdeliverable:

31October2017

Actualsubmissiondate:

30October2017

Version: Final

MainAuthors: BehzadMostajir,SébastienMas,DavidParinandFrancescaVidussi(Partner10CNRS-MARBEC)

ThisprojecthasreceivedfundingfromtheEuropeanUnion’sHorizon2020researchandinnovationprogrammeundergrantagreementNo732065

DisclaimerThis deliverable is a living document and will be updated as project progresses. New versions will beavailableandpublishedontheprojectwebsite:www.aquacosm.eu

ThisprojecthasreceivedfundingfromtheEuropeanUnion’sHorizon2020researchandinnovationprogrammeundergrantagreementNo732065

Projectref.number 731065

ProjecttitleAQUACOSM:NETWORKOFLEADINGEUROPEANAQUATICMESOCOSMFACILITIES

Deliverabletitle ReportonthecurrentstatusandaimsforLAMPSensorSystem

Deliverablenumber D8.2

Deliverableversion Final

Contractualdateofdelivery 31October2017

Actualdateofdelivery 30October2017

Documentstatus Public

Documentversion Final

Onlineaccess Yes

Diffusion PUBLIC

Natureofdeliverable

Workpackage WP8

Partnerresponsible CNRS-MARBEC

Author(s) BehzadMostajir,SébastienMas,DavidParinandFrancescaVidussi(Partner10)

Editor

Approvedby JensNejstgaard(IGB)

ECProjectOfficer AgnesRobin

Abstract

Keywords LAMPSensorSystem,automatedsensors,temperature,conductivity,oxygen,fluorescenceofchlorophyll,light,highfrequencydata

D8.2ReportonthecurrentstatusandaimsforLAMPSensorSystem

AQUACOSM – INFRA-01-2016-2017- N. 732065 of 13

Co-fundedbytheEuropeanUnion

TableofContents

1. Executivesummary.......................................................................................................5

2. CurrentstatusandaimsforLAMPSensorSystem........................................................5

2.1 CurrentstatusofLAMPSensorSystem...............................................................5

3. DisseminationactivitiesrelatedwiththeDeliverable................................................13




1. ExecutivesummaryInmesocosm studies, generallywater is sampled for various analyseswith theobjective to study theeffectofvariousexperimental manipulationsonthebiologicalassemblagesandchemicalparameters.Theaddedvaluesofusing sensors in themesocosmsare firstly toprovide information in real time for somephysical, chemical andbiologicalparametersdirectlyonuntouchedwater inthemesocosmsundervariousexperimentaltreatments,andsecondlytomonitortheseparameters inhightemporalresolution.Todothis,partner10CNRS-MARBECduringthe FP7 European project MESOAQUA developed a system (LAMP Sensor System) consisting of commercialsensors combined with electronic devices and adapted software to obtain high frequency data which istransmittedtoahubinrealtimeviaRF.In the frame of AQUACOSM project the LAMP Sensor System and associated data loggers will be furtherimplementedtoamorerobustandenhancedsystemwiththepossibilitytoincludenewsensorsandtomonitoruptosixmesocosms.ThefirstobjectiveofLAMPSensorSystemdevelopmentandenhancementistobeabletominiaturize this system and adapt it to mesocosms with different diameters, including the new low-costAQUACOSM,whichiscurrentlyindevelopmentinWP7.Thesecondaimistotesttheadaptabilityofsensors,datalogger and electronic components of this system to different conditions in the laboratory and in naturalmesocosm systems from natural Mediterranean marine environment to cold (around and below 0°C) water.ParticulartestingscenariosincludewinterconditionsinUmeå,Sweden,duringwinter2018.Moreover,theLAMPSensorSystemwillbeemployedandtestedintheframeofWP9inajointmesocosmexperimentinSvalbard.In this report,wepresent thecurrent statusof theLAMPSensorSystem and thedetailofdifferent stepsandtests to go ahead toward the enhanced LAMP Sensor System which will be also adapted to operate in coldenvironments.

2. CurrentstatusandaimsforLAMPSensorSystem

2.1 CurrentstatusofLAMPSensorSystem

LAMP Sensor System comprises i) a pack of sensors, ii) mixing system, iii) data acquisition, storage andtransmissionsystem,andiv)powersystem.ThesensorPackcomprisestemperature,conductivity,oxygenandChlorophyllafluorescence(Chla)sensors(Fig.1).




Figure1.LAMPSensorPack.

Apumpinsuresthegentlemixandhomogenizationofwatercolumninthemesocosm.Theturnoverrateofthispumpcanbecontrolledandadjusted.Acquisition, storageand transmissionofdata areundertakenbyeachonedata loggerpermesocosm to firstlystoreall data fromeachmesocosmand secondly to transmit thedata toapowerful centraldata loggerwhichcollects data from all mesocosms. This latter central data logger is established on an “in situ Observatory”platformwhichisshowedinFigure2.




Figure2.DetailofLAMPinsituObservatory.

LAMP in situObservatoryplatform comprises aweather station and apackof sensor (temperature andChla)immergednotinthemesocosmbutinthenaturalsurroundingwaterofthemesocosms.Asolarpanelprovidesthe necessary power for immerged sensors, weather station and themain data logger. Themain data loggertransmitsthedataatrealtimefromallmesocosmsandfromtheinsituobservatorytoaremotePCbasedonlandoronashipinthevicinityoftheLAMP.Figure 3 shows a general view of the LAMPwith the position of sensors,mixing system, data loggers, in situObservatorysystemandpowersupplies.




Figure3.SchematicpresentationofLAMPanditssensorsystem.

LAMPanditsSensorSystemweresuccessfullytestedintheMediterraneanSea(Fig.4,Mostajiretal.2013,L&O:Methods394-409).

Figure4.LAMPdeploymentintheBayofDiaIslandintheCretanSea(Mostajiretal.2013).




2.2AimforLAMPSensorSystemdevelopmentinAQUACOSM

2.2.1Choiceofthesensors

TheaimofLAMPSensorSystemdevelopmentduringAQUACOSMprojectwasexplainedinthesummaryofthisreport.Toreachtheseobjectives,thechoiceofthebestsensorswasthefirststep.FromthebeginningoftheAQUACOSMproject,Partner10studieddifferenttypesofsensors.AstheLAMPSensorSystemwasdevelopedforsensorstobeimmergedinthewatercolumnofthemesocosms,chemicalsensors,i.e.with chemical reactive that could pollute themesocosmwere excluded. As an example, sensors thatmeasurenutrientsinvolvingreagentsandcolorimetricmethodsarenotconsideredhere.

TodemonstratetheLAMPSensorSystemanddiscussdevelopmentswithWP8AQUACOSMPartners,Partner10organizedaWorkshopspecificallydedicatedtotheLAMPSensorSysteminSète,Francefrom19to20June2017.

2.2.1.1WorkshopontheLAMPSensorSystem,Sète,France,19-20June2017

Theparticipantsonthisworkshopwere:partners5(RF-Sens),10(CNRS-MARBEC),12(WCL)and16(SYKE).Duringthisworkshop,LAMPSensorSystemwasdemonstratedanddiscussedthoroughly.TheprogramofthisWorkshopwasasfollow.

Monday19June2017

- 09h30–12h00.DemonstrationofLAMPSensorSystembydeployingamesocosmtothelagoonincludingtheLAMPSensors.StartingthepresentationoftheLAMPSensorsbypartner10(CNRS-MARBEC).AsetofLampSensorswillbeavailableintheconferenceroom.

- 14h00 – 18h00. Continue the presentation of the LAMP Sensors by partner 10: Characteristics,calibrations,bestpracticeandstandardprocedures(linkwithWP2and4).PresentationofsensorswhichwillbeacquiredbyPartner10intheframeofAQUACOSMproject.Continuediscussion.

Tuesday20June2017

- 09h00–12h00.PresentationofLAMPSensorSystemimprovementbyPartner10.- Presentation of the data acquisition, management, etc. of the LAMP Sensor System and its present

configurationbyPartner10.- PresentationofRF-SENSandtheirpotentialroleintheWP8andpropositionsbyPartner5.

- 14h00–18h00.DiscussiononLAMPdevelopmentcontinues,especiallyregardinglinkstoWP7&WP9(all

partners).

- Commonsensors&parametersbetweenLAMPSensorSystem&AQUABOXbypartners10&16(SYKE)

- PresentationofprogresswithChemostatsetupbyPartner12(WCL)

2.2.2Acquisitionofthenewsensors




ThechoiceofnewsensorstofurtherdeveloptheLAMPSensorSystemwasbasedonthoroughdiscussions aboutcharacteristicsofavailablesensorsbetweentheparticipantsinWP8fromthebeginningoftheproject,includingduringtheWorkshopinSète(June2017).ThefinalchoiceofsensorswascommunicatedtoallpartnersinWP8inordertoensurecomparabilityofsystemsdevelopedinWP8.2.1(LAMPSensorSystem)and8.2.2.(AquaBox).Regarding the budget allocated to Partner 10 for acquisition of the sensors (50000 Euros), 6 Packs of sensorshavebeenpurchasedanddelivered.Eachpackofopticalandphysical,chemical,andbiologicalsensorsincludesthefollowingsensors:

• Three temperature sensors. Thermistor probe 107, Campbell Scientific Ltd., range: - 35 to +55°C,accuracy:<0.2°C.Theycanbeinstalledatsurface,middleandbottomofthemesocosm.

• Anelectromagnetic inductionconductivitysensor.AanderaaData InstrumentAS, range:0-75mS/cm,accuracy:±0.018mS/cm.

• AnoxygenOptode sensor.AanderaaData InstrumentAS, range:0-500µMandaccuracy:<8µM foroxygenconcentration,range:0-150%andaccuracy:<5%forairsaturation.

• An ECO-FLrt chlorophyll fluorometer. WETLabs/Sea-Bird scientific, 470/695 nm Ex/Em, range: 0-50µg/L,accuracy:0.02µg/L.

• A LI-193SA Spherical underwater quantum sensor. LI-COR Biosciences GmbH, measuringphotosyntheticallyactiveradiation(PAR:400-700nm)fromalldirections.

Thepackofsensorscanbeimmergedatthemid-depthofthemesocosmandacquireshighfrequencydataduringthemesocosmexperiment.RelativetopreviousLAMPSensorSystem,weaddaALI-193SASphericalunderwaterquantum sensor to the sensor pack in order to monitor also the PAR received at the middle depth of themesocosm. This information combined with those come from the meteorological station, which monitor theincident PAR received at the surface of themesocosm, allows the calculationof PAR attenuation in thewatercolumnofthemesocosmequippedwiththeLAMPSensorSystem.2.2.3Improvementofdataacquisitionandstoragesystem

Presently,alldatacollectedbythesensorsmonitoringeachmesocosmandthoseofthe“insituObservatory”aresaved inan independentdata logger (CR1000).Eachdata loggerthensendalldatatoahubcollector (CR3000)thatsaveandtransmitthemtoaremotePConanearbyvesseloronland.

Theacquisition-controlsystemsavingdatacollectedbysensorsofeachmesocosm(includingthoseofthe“insituObservatory”) comprise adata logger for storage (CR1000,Campbell), a data radio-transmissionmodule andaturnover control module for the pump. All connections with probes are waterproof and all of this system isprotectedbyarelativelylargePVCtube(60cmlongand9cmdiameter).

DuringtheactivityofWP8thepresent/previousacquisition-controlsystemwillbeenhancedbyminiaturization,usingmicrocontrollerbasedonArduinoandadatabluetoothtransmissionmodule.Theturnovercontrol module




and the electric converter remain unchanged. In this way, the acquisition-control systemwill be considerablyreduced.

A

B

Figure5.Theacquisition-controlsystem.A)Presentsystem(60x5cm)whichwillbeminiaturizedusing(B)themicrocontrollerbasedonArduinoanddatabluetoothtransmissionmodule.

2.2.4Improvementofpowersupply

ThedetailforenergyconsumptionoftheLAMPSensorSystemforevery2minutespathsamplingis:

- Forthesensorsabout2to3mA,- Forthedataloggerabout28mA,- Forthemeteorologicalstation0.15mA,and- Forthepumpinitsmaximalflowrate1A.

Thepumpconsumesthemostenergy.WhentheLAMPSensorSystemwheredeployedinthesea,acarbatterywithacapacityofabout60Awasused,andhadtobechangedevery2daystoprovideenoughpower.Thepumpassuringthemixingandhomogeneityofwatercolumnof themesocosmwhichallowsfixingthesensorpackatmiddepthofthemesocosmtherefore;dataacquiredarerepresentativeofthemesocosmwatercolumn.

Theuseofapump for thewatercolumnmixingcanbeneglected if theLAMPSensorSystem is installed inanenvironment inwhich themesocosmwater columnmixing is assuredby thenaturalwavesof the surroundingwaterofthemesocosm,orbyanothermixingmethodwhichdoesnotneedanhighenergysupply.Ifthepumpisnotused,theLAMPSensorSystemcanbesuppliedbyabatteryofanormalcellulartelephoneorofaDronewiththecapacityofabout5Aduringalmost7days.

2.3WorkplanforadaptationofLAMPSensorSystemtocoldconditions




As explainedpreviously, the current LAMPSensor Systemanddata acquisition-transmission systemhavebeensuccessfully tested in theMediterraneanenvironments.However, theLAMPSensorsandassociatedelectronicshaveneverbeentestedatlowtemperaturesinparticulararound0°CwhichisoneoftheaimsoftheAQUACOSMproject.

Currently,allnewsensorshavebeenpurchasedbyPartner10anddeliveredandsomeitemsofthebelowWorkPlanareunderachievement.

WorkPlanforfall2017andfortheyear2018consistson:

- acquisitionofelectronicandothersmallcomponents- cablingthesensors- testofeachreceivedsensor- designanddoconstructthesensorsupportslikethatofCTDsensorsupport- assemblageofsensorsasapackaroundsensorsupport- testofLAMPSensorSystem(withoutpump)withthenewpowersupply- test of good functioning of LAMP Sensor System for each of sensor pack and data

acquisition/transmissionsysteminthelaboratoryusingwaterwithambientwatertemperature- testoffunctioningofLAMPSensorSysteminthelaboratoryexposingittocoldwater(byadditionofice)- testoffunctioningofLAMPSensorSystembyputtingthecomponentsinthefreezer!- testofgoodfunctioningofLAMPSensorSysteminthenaturalMediterraneanwater(lagoon)forseveral

days.

If the results of these tests for the LAMP Sensor System simulating the cold water and cold weather in thelaboratoryaresuccessful,thenexttestwillberealizedinnaturalcoldwaterconditions.

Partner 1 and collaborators is constructing currently the new low-cost AQUACOSMmesocosm in the frameofWP7. Conjointly with other partners, we (partner 10) envisage to install the LAMP Sensor System in anAQUACOSMmesocosmduringwinter2018torealizeseveraldaysofhighfrequencydataacquisitioninanaturalcoldwaterandcoldweatherinUmeåinSweden.

Aspreviouslymentioned,ultimatelyLAMPSensorSystemwillbetestedintheframeofWP9injointmesocosmexperimentinSvalbard.

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3. DisseminationactivitiesrelatedwiththeDeliverablePleasereferto2.2.1.1.

AlsowithintheframeworkoftheWorkshoponLAMPSensorSystem,heldatSète,France,19-20June2017,newsabout the workshop were communicated through the normal social media (Twitter) and featured in theAQUACOSMNewsletter01.