Dalton Cardiovascular Research CenterJanuary 2018

Brian Ruyle, Biomedical Sciences Graduate student in the Laboratory of Dalton Resident Investigator, Dr. Eileen Hasser, has received a Re-search Fellowship award from NIH, Project Title: Role of PVN neuropep-tide projections to the nTS in cardio-respiratory control.

This award will allow Brian to contin-ue his research for the 2018 & 2019 year. Congratulations Brian!

Brian Ruyle receives Research Fellowship Award

Kendall Annetti graduates with an MS in Biomedical Sciences

Kendall Annetti, a graduate student in Laboratory of Dr Lane Clarke, Dal-ton Resident Investigator, graduated with an MS in Biomedical Sciences this past summer. This is after receiv-ing her DVM last spring.

Kendall is currently in residency in Veterinary Pathology at the University of Minnesota.

Congratulations to Kendall!

online communicate-on-demand tool.

Here is the direct link to Dr Polo-Parada’s piece: https://missouri.box.com/s/b2cekyxpsjt3vc1pk8eoykaviwwzfzk6

Dr Polo-Parada worked with Gayle Mooney in the Mizzou Advantage to create this University Marketing Piece.

If you have ideas for marketing your research, you can email: muprovostmizzouadv@missouri.edu.

The communicate on demand tool can be seen here: https://mizzouadvantagecod.com/

Skin in the Game

Dr Luis Polo-Parada, Resident Dalton In-vestigator, and Asso-ciate Professor, De-partment of Medical Pharmacology and Physiology has creat-ed This piece, Skin in the Game, and is now available for use by all of campus via the

25th Annual Cardiovascular Day

The 25th Annual Cardiovascular Day will be held on February 27, 2018, in the Reynolds Alumni Center on the campus of the University of Missouri in Columbia, Missouri. Cardiovascular Day provides an opportunity to share information with colleagues, students, University community, and area physicians about the important cardiovascular research conducted both on campus and in the region.

The 2018 J O Davis Keynote Speaker is Andrew D. McCulloch, PhD, Distinguished Professor of Bioengineering and Medicine University of California San Diego. Click here for further details: http://dalton.missouri.edu/func-tions/cvday/cv25.php

Brian Ruyle

Kendall Annetti

MU researcher closes in on pathways involved in ALS diseaseFindings could lead to improved diagnosis and drugs for the treatment of

Lou Gehrig’s disease, stroke recovery

Nov. 30, 2017 Contacts: Jeff Sossamon, Science Writer, (573) 882-3346, sossamonj@missouri.edu COLUMBIA, Mo. – It is estimated that between 14,000 and 15,000 Americans have amy-otrophic lateral sclerosis (ALS), according to the National Institutes of Health. Symptoms of ALS, also known as Lou Gehrig’s disease, may be subtle at first, but develop into more obvious muscle weakness and paralysis. Recently, a University of Missouri researcher identified a potential target for therapeutics that may help to lessen the severity and progression of ALS. Researchers suggest that this same enzyme pathway also could help in the recovery of patients who have suffered strokes and other disorders.

“ALS is a motor neuron disease. It begins with the degeneration of upper and lower motor neurons, and leads to muscle atrophy, paralysis and eventually death,” said Shinghua Ding, an associate professor of Bioengineer-ing and an investigator in the Dalton Cardiovascular Research Center. “Our previous studies indicated that an enzyme known as NAMPT (nicotinamide phosphoribosyltransferase) is primarily expressed in the neurons in mouse model, and overexpression of NAMPT can protect against further brain injury following a stroke. For these reasons, NAMPT became a good target of study.”

Ding and his team first observed that mice lacking the NAMPT enzyme led to progressive loss of weight, hypo-thermia, motor neuron degeneration, and motor function deficits; most of which mirror the symptoms that are observed in humans with ALS.

Then the team treated the mice with a product that regulates NAMPT activity. The molecule is called nicotin-amide mononucleotide, or NMN, and serves as a substitute for the missing enzymatic product. Mice treated with the NMN molecule exhibited enhanced motor neuron function and overall improved health. Importantly, they demonstrated that NAMPT levels were significantly reduced in the spinal cord of ALS patients. Their dis-covery indicates that NAMPT is involved in the development of ALS.

“What we’ve shown is that NAMPT is essential to neuronal function and viability,” Ding said. “Remarkably, NMN improved health, restored motor function and extended the lifespan in NAMPT-deficient mice. Based on our findings, it is an ideal candidate for further study, and the possible development of drugs in the diagnosis and treatment of ALS and stroke victims.”

The study, “Deletion of Nampt in Projection Neurons of Adult Mice Leads to Motor Dysfunction, Neurodegen-eration, and Death,” recently was published in Cell Reports. Funding was provided by the National Institutes of Health (Grants: R01NS069726, R01NS094539, P30AG035982 and 1R01NS089604) and an American Heart Association Grant in Aid award. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies.

The Vevo LAZR Photoacoustic Imaging System and Rodent Cardiovascular Phenotyping Facility are ready for your use. The overall goals of the facility are:

• To establish a core rodent cardiovascular phenotyping facility for the use of MU Investiga-tors to characterize the in vivo consequences of genetic and disease-based manipulations• To provide fee-for-service capabilities so as to decrease the needs of individual laboratories to establish their own testing facilities and provide consistency/quality control of measurements• To provide a comprehensive core facility that will value-add to the programs of individual investigators and increase competitiveness for both external grant funding and interactions with industry.

To discuss or schedule use, please contact, Zhe Sun PhD, sunzh@missouri.edu, or (573) 884-2499

Vevo LAZR Photoacoustic Imaging System and Rodent Cardiocascular Phenotyping Facility

PUBLICATIONS

Endothelial dysfunction occurs independently of adipose tissue inflammation and insulin resis-tance in ovariectomized Yucatan miniature-swine. Jurrissen TJ, Olver TD, Winn NC, Grunewald ZI, Lin GS, Hiemstra JA, Edwards JC, Gastecki ML, Welly RJ, Emter CA, Vieira-Potter VJ, Padilla J. Adipocyte. 2017 Dec 28:1-10. doi: 10.1080/21623945.2017.1405191. [Epub ahead of print] PMID: 29283284

Cardiovascular disease progression in female Zucker Diabetic Fatty rats occurs via unique mechanisms compared to males. Lum-Naihe K, Toedebusch R, Mahmood A, Bajwa J, Carmack T, Kumar SA, Ardhanari S, DeMarco VG, Emter CA, Pulakat L. Sci Rep. 2017 Dec 19;7(1):17823. doi: 10.1038/s41598-017-18003-8. PMID: 29259233

Cystic fibrosis research topics featured at the 14th ECFS Basic Science Conference: Chair-man’s summary. Mall MA, Hwang TC, Braakman I. J Cyst Fibros. 2017 Dec 9. pii: S1569-1993(17)30965-7. doi: 10.1016/j.jcf.2017.11.008. [Epub ahead of print] PMID: 29229473

Voluntary wheel running improves adipose tissue immunometabolism in ovariectomized low-fit rats. Zidon TM, Park YM, Welly RJ, Woodford ML, Scroggins RJ, Britton SL, Koch LG, Booth FW, Padilla J, Kanaley JA, Vieira-Potter VJ. Adipocyte. 2017 Dec 11:1-15. doi: 10.1080/21623945.2017.1402991. [Epub ahead of print] PMID: 29226756

Remote Activation of a Nanopore for High-Performance Genetic Detection Using a pH Taxis-Mimicking Mechanism. Wang Y, Tian K, Du X, Shi RC, Gu LQ. Anal Chem. 2017 Dec 19;89(24):13039-13043. doi: 10.1021/acs.analchem.7b03979. Epub 2017 Dec 4. PMID: 29183111

What is the potential of nanolock- and nanocross-nanopore technology in cancer di-agnosis? Gu LQ, Gates KS, Wang MX, Li G. Expert Rev Mol Diagn. 2017 Dec 1:1-5. doi: 10.1080/14737159.2018.1410060. [Epub ahead of print] No abstract available. PMID: 29171309

Nanopore electric snapshots of an RNA tertiary folding pathway. Zhang X, Zhang D, Zhao C, Tian K, Shi R, Du X, Burcke AJ, Wang J, Chen SJ, Gu LQ. Nat Commun. 2017 Nov 13;8(1):1458. doi: 10.1038/s41467-017-01588-z. PMID: 29133841

134 Research Park DriveColumbia, MO 65211

573-882-9482dalton.missouri.edu

PUBLICATIONS

Dr Kevin Gillis, Resident Dal-ton Investigator and Professor Biological Engineering and Medical Pharmacology and Physiology has received noti-fication of a Patent Issuance. Patent No: 9,696,275 on the technology entitled

Dr Li-Qun Gu, Dalton Resident Investigator and Associate Pro-fessor, Bioengineering receives Patent Number 9,732,379 on the technology entitled "Encod-ed Nanopore Sensor for Multi-plex Nucleic Acids detection"

Dr Kevin Gillis issued Patent Dr Li-Qun Gu issued Patent

Ion channels as targets to treat cystic fibrosis lung disease. Martin SL, Saint-Criq V, Hwang TC, Csanády L. J Cyst Fibros. 2017 Nov 6. pii: S1569-1993(17)30917-7. doi: 10.1016/j.jcf.2017.10.006. [Epub ahead of print] MID: 29102290

Interaction of IL-6 and TNF-α contributes to endothelial dysfunction in type 2 diabetic mouse hearts. Lee J, Lee S, Zhang H, Hill MA, Zhang C, Park Y. PLoS One. 2017 Nov 2;12(11):e0187189. doi: 10.1371/journal.pone.0187189. eCollection 2017. PMID: 29095915

Prolonged leg bending impairs endothelial function in the popliteal artery. Walsh LK, Restaino RM, Martinez-Lemus LA, Padilla J. Physiol Rep. 2017 Nov;5(20). pii: e13478. doi: 10.14814/phy2.13478. PMID: 29061865

Imaging of Mitochondrial and Cytosolic Ca2+ Signals in Cultured Astrocytes. Zhang N, Ding S. Curr Protoc Neurosci. 2018 Jan 22;82:2.29.1-2.29.11. doi: 10.1002/cpns.42. PMID: 29357111

Biophysical properties of microvascular endothelium: Requirements for initiating and conducting electrical signals. Kapela A, Behringer EJ, Segal SS, Tsoukias NM. Microcirculation. 2017 Nov 8. doi: 10.1111/micc.12429. [Epub ahead of print] PMID: 29117630

Lessons learned from participating in D3R 2016 Grand Challenge 2: compounds targeting the farne-soid X receptor. Duan R, Xu X, Zou X. J Comput Aided Mol Des. 2018 Jan;32(1):103-111. doi: 10.1007/s10822-017-0082-x. Epub 2017 Nov 10. PMID: 29127582

“Addressable Electrode Arrays in Multiple Fluidic Compartments and Uses Thereof"