Day Date Reading Topic

Th 11/28 no class – Thanksgiving BreakT 12/3 Ch. 12 more on NeurogenesisTh 12/5 Ch. 9 The Saga of the Germ LineT 12/10 more on Reproduction quiz on Tues: Draw and Describe pathfinding in any animal CNSTh 12/12 don’t need to read Ch. 13 Growth  in class assignment on Thurs: build an Edible Urbilaterian Ancestor you can bring extra ingredients

The Final Exam will be in our regular classroom on Friday, Dec. 20th, 8:00 – 10:00 AM. The exam will cover all the material in the course.

Also remember there are two, short writing assignments, each worth 20 points. Pick any topic in developmental biology (except your term paper topic) and learn more about it. Then write a two page paper and include all the sources you used (those can include any sources including web sites, newspapers, TV, etc). These are due anytime.

Final Exam covers the whole course

Made from Fig. 12.25

12.18 growth cone guidance

Fruit fly embryonic CNS

wild-type robo mutant comm mutant slit mutant (roundabout) (commissureless) (I faked this pic)

http://en.wikipedia.org/wiki/Urbilaterian

http://palaeos.com/metazoa/bilateria.htm

Chapter 9: Reproduction

The Saga of the Germ line cells FertilizationSex determinationDosage compensation

9.6 embryonic migration of germ line cells in mammals

migrating mouse germ line cells tagged with GFP

1) We learned we can make a reporter gene construct by fusing any gene promoter of interest to the GFP transcription unit. When fused to the insulin promoter, for example, the hybrid gene would be in every cell but only differentiated cells of the pancreas glow.

1) We learned we can make a reporter gene construct by fusing any gene promoter of interest to the GFP transcription unit. When fused to the insulin promoter, for example, the hybrid gene would be in every cell but only differentiated cells of the pancreas glow.2) We can use the same construct and kind of flip it’s purpose. The insulin promoter is a “tissue-specific” promoter that let’s us tag a specific cell type.

migrating mouse germ line cells tagged with GFP

migrating mouse germ line cells tagged with GFP

Developing kidney in a mouse strain tagged with GFP constructs.

migrating mouse germ line cells tagged with GFP

9.6 germ cell migration

9.20 SRY on Y chromosome

These are all somatic cells, including the cells that makes estrogenand testosterone. The germline cells make eggs or sperm in responseto whether they are in a testes or ovary (nonautonomous).

9.23 fly gynandromorph

Compare sex determinationin people (vertebrates) (nonautonomous regulated by steroid hormones) to sexdetermination in fruit flies(cell autonomous = each celldecides if it is an male orfemale).

9.24 fly sex determination pathway

9.25 sex lethal feedback

9.21 mammal gonad development

Every embryo starts with bipotential somatic gonads.

WRASSE

During germ line cell migration, cells erasemuch of theirDNA methylation / imprinting / epigenome.

Figs 9.11/12 imprinting

Chapter 9: Reproduction

The Saga of the Germ line cells FertilizationSex determinationDosage compensation

In the nucleus of each of their cells, women have a Barr body.It is one of her two X chromosomes that has been packed awayand isn’t used.

dosage compensation

In the nucleus of each of their cells, women have a Barr body.It is one of her two X chromosomes that has been packed awayand isn’t used.

dosage compensation = Men have just one X and Women effectively have just one X, so the level of gene expressionis the same in men and women.

9.30 three pathways for dosage compensation

9.33 three pathways for dosage compensation

9.26 nematode sexes

Chapter 9: Reproduction

The Saga of the Germ line cells FertilizationSex determinationDosage compensation

9.8 meiosis review

9.9 gamete maturation

9.9 gamete maturation

Chapter 9: Reproduction

The Saga of the Germ line cells FertilizationSex determinationDosage compensation

9.14 fertilization

9.15 egg cell membrane depolarization

9.16 cortical granules

9.17 intracellular Ca++ wave