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DECALCIFICATION SOLUTIONS Acid Reagents Formic Acid Goodings and Stewart’s Fluid Formic Acid (90%) - 100mL Formalin - 50mL Distilled Water - 850mL Formic Acid-Sodium Citrate Solution Formic Acid - 35mL 20% Sodium Citrate - 65mL 10% Aqueous Formic Acid Solution Nitric Acid Nitric Acid Formaldehyde Nitric Acid - 10mL Formaldehyde - 10mL Distilled Water - 100mL 5-10% Aq Nitric Acid Perenyi’s Fluid 10% Aq Nitric Acid - 40mL Absolute Alcohol - 30mL 0.5% Chromic Acid - 30mL Hydrochloric Acid Von Ebner’s Fluid Conc Hydrochloric Acid - 15mL Sodium Chloride - 175g Distilled Water - To 1000mL

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Solutions for Decalcification

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Page 1: Decalcification Solutions

DECALCIFICATION SOLUTIONS

Acid Reagents

Formic Acid

Goodings and Stewart’s Fluid

Formic Acid (90%) - 100mL

Formalin - 50mL

Distilled Water - 850mL

Formic Acid-Sodium Citrate Solution

Formic Acid - 35mL

20% Sodium Citrate - 65mL

10% Aqueous Formic Acid Solution

Nitric Acid

Nitric Acid Formaldehyde

Nitric Acid - 10mL

Formaldehyde - 10mL

Distilled Water - 100mL

5-10% Aq Nitric Acid

Perenyi’s Fluid

10% Aq Nitric Acid - 40mL

Absolute Alcohol - 30mL

0.5% Chromic Acid - 30mL

Hydrochloric Acid

Von Ebner’s Fluid

Conc Hydrochloric Acid - 15mL

Sodium Chloride - 175g

Distilled Water - To 1000mL

Page 2: Decalcification Solutions

Jenkin’s Fluid

Absolute Alcohol - 73mL

Distilled Water - 10mL

Chloroform - 10mL

Glacial Acetic Acid - 3mL

Hydrochloric Acid - 4mL

Trichloroacetic Acid

Trichloroacetic acid - 5g

10% Formal Saline - 95mL

Ion Exchange Resins

WIN 3000 - 100g

10% Formal Saline - 95mL

Buffer Solutions

Citric Acid Citric buffer

7% aq Citric Acid - 5mL

7.54% aq Ammonium Citrate - 95mL

1% Aq. Zinc Sulphate - 0.2mL

Chloroform (as Preservative) – 2-3 drops

Chelating Agent

EDTA - 55g

Formalin - 100mL

Distilled Water - 900mL

Electrolyte Solution

88% Formic Acid - 100mL

Hydrochloric Acid - 80mL

Distilled Water - 820mL


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