demo and proposition us - 3dhistech ltd

Demo and proposition US

Péter Tóth, 3DHISTECH Ltd.

• More than 200 systems

• Contract with Thermo Fisher in 2017

• FDA: in progress

3DHistech in US

• Reached $100M in 2017

- Largest share of the global market

- To be doubled by 2020

• Nearly 200 scanners have been placed last year

• Biggest players: Leica, Philips, 3DHISTECH, Roche, Sakura, Hamamatsu, Zeiss

US digital pathology market

US digital pathology market share 2017

More than 90% of the scanners sold by these companies

60% of the overall spending on digital pathology

35-40M taken by software companies, storage providers, other scanner companies

• High-throughput: speed and stability in scanning

• Many kind of slides: scanning technology, sample detection (scan profiles)

• Complexity: connection and integration

• Storage

• Multi-site projects: IT infrastructure

You need to prove all of these

Challenges in clinical demo

• Clinical customer in the Midwest

• Providing laboratory services for 26 hospitals and thousands of physician offices

• 4000 slides/day

• Million dollars spent on courier services

• FDA news GO

Clinical project 1

Project:

• 8-10 pieces of P250

• Case Center starting with50 users

• Case Viewer

• Integration

Clinical project 1

Demo:

• Leica, Philips, 3DH, Hamamatsu

• 1000 BF slides to scan on-site

• Massive evaluation

• Parallel slide comparison

• Site visits

• Scan success rate: 1000/1000(P250)

• Very good scan times• Image quality comparable or

better• Slide handling in CV

Clinical project 1

• Slide management• Missing workflow sw

Clinical project 1

The solution is the CaseManager

New slide management framework

Laboratory Information System

Digital microscope application

Complete digital pathology workflow

Clinical project 1

Clinical project 2 – Memorial Sloan Kettering

• Cancer treatment and research institution in New York City

• Long history. First scanner delivered in 5-6 years ago

• Strategic partnership

Molecular Cytology Core Facility

• P250

• SCAN

• MIDI

• Confocal

• iSaCS


Department of Pathology (Cytology)• P250• Confocal• CoPath – CC integration


CoPathPlus

CaseViewer

P250CaseCenter

CaseViewer QuantCenter

Confocal

ConfocalCaseManager

?CaseViewer


Challenges in MSK

• Integration: maintenance and support• Scanning cytology slides with P250

40x + single layer: 5-10 mins 40x + multilayer: 5-10 mins * number of

layers

Solution: P1000 • Scanning cytology with P1000 40x + single layer: 5 mins 2.5-5

mins 40x + multilayer: FLASH! Immersion

• Fluorescence scanning

• Filters

• Demo situations• Bad staining

• What staining? Prepare your own slides

• Weak signal

• Picky customer

• Smaller projects, lower sales volume

Challenges in research demo

Research areas US

Boston-Cambridge

San Francisco Bay Area

World's Largest Biotech Hubs

Boston Cambridge – SF Bay area

• Largest concentration of biotech activity in the world• Employ 50,000 people in bioscience-related jobs• 200 (Boston) vs 240 (SF) companies• Cambridge is more narrowly focused on pharmaceutical and biotech drug

discovery. Sanofi, Pfizer, Biogen-Idec, Novartis• Almost half of all US biotech investment is made in these areas• About 1/3 of the biotechnology employees work for companies in these areas and

about a quarter of US biotech companies are located in one of these clusters

Research project – Advanced Cell Diagnostics

• SF Bay Area• Primarily a probe and reagent products company + significant Pharma Assay

Services business• Proprietary RNA in situ hybridization (RNA ISH) assay, called RNAscope• Run both chromogenic and fluorescence RNAscope assays• Total volume is nearly 15,000 slides a year run in RNAscope services. They scan all

of these slides• Leica and Zeiss scanners• Fluorescence scanning is a major bottleneck and they needed higher throughput

scanners


ACD Demo

• SCAN II FL vs Zeiss AxioScan

• Mouse brain tissues stained with FITC, Cy3, Cy5 RNAscope®

Multiplex Fluorescent Assay

• Speed factor 1 layer, 20x objective

• ~2x faster than AxioScan


RNAscope® Multiplex Fluorescent Assay


ACD conclusions

• Focus on customer requirements

• Fluorescence Selling point

• Extending their application: Opal 4-plex 520/570/620/690

• Strategic partnership

Research project - BlueBirdBio

Chromogenic In Situ Hybridization (RNAscope) at BlueBirdBioDAPI, FITC, Cy3, Cy5 Quad-band

Research project - Merck

• Merck Anatomic Pathology Group, Imaging & Quantitative Analysis

• Fast brightfield scanning (H&E, IHC) + fluorescence (Opal 7-plex)

• Hamamatsu S60, Zeiss AxioScan

Research project - Merck

• Opal 7-plex. Filter set was not available Now it is!

• Fluorescence workflow, sample detection Scanner software v2.0

• Budget issue More active follow up on pricing

Merck conclusions:

Research

Opal 7-plex

Opal Dapi - LED DAPIOpal 520 - FITC-5050A !Opal 540 - LED-YFP-AOpal 570 - Tritc-BOpal 620 - LEDmCherryOpal 650 - LED Cy5Opal 670 - Cy5-4040COpal 690 - Cy5.5 !

Research

Thank you for your attention

demo and proposition us - 3dhistech ltd

Documents