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Demonstration of Artemia decapsulation

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Background

Alveolar layer of the cyst are removed

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Why decapsulated cysts?A complete contamination free and sterile

productSeparation problems after hatch are

avoided

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Preparation

Tank set-up with stainless steel filter and aeration system

Addition of water and strong aeration

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Hydration

Dry cysts are hydrated in water with strong aeration

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Addition of chlorine and caustic soda => increases pH above 10

Addition of chlorine and hypoclorite solution

Temperature should be maintained within 25-30C

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Colour change

1. Dark brown2. Grey3. OrangeThe process is stopped immediately after orange

colour appear

Process lasts from 5-15 min

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Checking of bouyancy

Decapsulated cysts will sinkNon-decapsulated cysts will float

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Termination of process Process is stopped by adding large amounts of water

until the smell of chlorine has disappeared

• Hypoclorite residue absorbed by the cysts is neutralized by 0.1 % Sodium Thio-sulfate

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Transfer to hatching tank