department of materials and optoelectronic science, national sun yat-sen university (nsysu)
DESCRIPTION
Antimicrobial Effects of Thin Film Metallic Glasses Deposited on 316L Stainless Steel. Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU). Student: Sunny Chu Advisor: Prof. J. C. Huang Date: 2012/11/13. Outline. Part 1 - Antimicrobial activity - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/1.jpg)
Department of Materials and Optoelectronic Science,National Sun Yat-Sen University (NSYSU)
Student: Sunny ChuAdvisor: Prof. J. C. HuangDate: 2012/11/13
Antimicrobial Effects of Thin Film Metallic Glasses Deposited on 316L Stainless Steel
![Page 2: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/2.jpg)
OutlinePart 1 - Antimicrobial activityIntroductionMotivationExperimental proceduresPreliminary resultsPart 2 - BiocompatibilityIntroductionMotivationExperimental proceduresPreliminary resultsFuture work 2
![Page 3: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/3.jpg)
Antimicrobial methods
1. Mechanisms of antimicrobial activity :Silver, Copper, Quaternary ammonium, Antimicrobial peptides
2. Selectivity:Bactericides, Viral inhibitors, Fungal inhibitors
3. Surface modification:Surface roughness, Superhydrophobic surfaces, Coatings (Self-cleaning coatings and Antimicrobial additives)
http://en.wikipedia.org en.wikipedia
3
![Page 4: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/4.jpg)
AFM observation316 stainless steel surfaces
As-received Electropolished for 1.5 min at room temperature
Electropolished for 5 min at room temperature
M. Haidopoulos et al., J. Mater. Sci. Mater. Med., 17, 647-657 (2006)
4
![Page 5: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/5.jpg)
Water contact angle test
Zr61Al7.5Ni10Cu17.5Si4 TFMG coatingsurface roughness: 1 nm
304 stainless steel substratesurface roughness: 7.5 nm
The flat surface was thought to improve its hydrophobic ability.
Chiang et al., Fooyin J Health Sci., 2, 12 (2010)
5
![Page 6: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/6.jpg)
Devasconcellos et al., Mater. Sci. Eng. C, 32, 1112-1120 (2012)
Antimicrobial activityPrevious reports have shown antimicrobial effects of materials with silver ions kill bacteria by destroying cell walls and membranes.
6
![Page 7: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/7.jpg)
Silver nanoparticles were shown to be an effective bactericide on E. coli.
I. Sondi and B. Salopek-Sondi, J. Colloid Interface Sci., 275, 177-182 (2004)
Antimicrobial activity
Containing different concentrations of silver nanoparticles: (a) 0 (b) 10 (c) 20 (d) 50 µg cm−3 7
![Page 8: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/8.jpg)
Particulate silver coatings on stainless steel implants for fracture management were shown to be an effective bactericide on Pseudomonas aeruginosa.
Antimicrobial activity
Devasconcellos et al., Mater. Sci. Eng. C, 32, 1112-1120 (2012)
8
![Page 9: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/9.jpg)
Escherichia coli (▲) Staphylococcus aureus (□)Pseudomonas aeruginosa (● ) Acinetobacter baumannii ( ) ◇Candida albicans ( )★
The surface of Zr61Al7.5Ni10Cu17.5Si4 thin film metallic glasses (TFMGs) can exhibit the antimicrobial ability on bacteria.
Antimicrobial activity
Chiang et al., Fooyin J Health Sci., 2, 12 (2010)
9
![Page 10: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/10.jpg)
Gram positive and gram negative
http://en.wikipedia.org en.wikipedia
10
![Page 11: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/11.jpg)
Motivation
1. To achieve good antimicrobial effects, the surface conditions of stainless steel can be improved by thin film coating.
2. Copper and silver ions were described as good antibacterial agents but copper is cytotoxic. Therefore, the materials with silver compositions can be utilized for the instruments in heath care.
11
![Page 12: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/12.jpg)
316L stainless steel
Mechanical polished Electropolished
AFM
Sputtering
AFM Nanoindenterα-step XRD
Biological assay
MTT assay
SEM EDS
Antimicrobial test
SEM
Contact angle
Flow chart
12
Glass
![Page 13: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/13.jpg)
MTT assay
http://en.wikipedia.org/wiki/MTT_assay
(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
Formazan
13
![Page 14: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/14.jpg)
AFM observationSubstrate: 316L stainless steel Surface treatment: grinded by #2000 sandpaperRoughness (Rms): 2.4 nm
14
![Page 15: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/15.jpg)
Substrate: 316L stainless steel Surface treatment: grinded by #4000 sandpaperRoughness (Rms): 2.3 nm
AFM observation
15
![Page 16: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/16.jpg)
Substrate: 316L stainless steel Surface treatment: 1. grinded by #180 sandpaper 2. electropolished (by MIRDC)Roughness (Rms): 1.5 nm
AFM observation
16
![Page 17: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/17.jpg)
Substrate: 316L stainless steel Surface treatment: 1. grinded by #600 sandpaper 2. electropolished (by MIRDC)Roughness (Rms): 1.1 nm
AFM observation
17
![Page 18: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/18.jpg)
Substrate: 316L stainless steel Surface treatment: 1. grinded by #1200 sandpaper 2. electropolished (by MIRDC)Roughness (Rms): 1 nm
AFM observation
18
![Page 19: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/19.jpg)
Thin film preparation
Fabrication method: Sputtering/co-sputtering processes
Substrates: (1) 316L stainless steel(2) Glass substrate
Thin films: Ag-based thin films
19
![Page 20: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/20.jpg)
Sputtering processMulti-gun sputtering system
Base pressure: 5 x 10-7 torr
Working gas: Ar, 30 standard cubic centimeters per minute (sccm)
Working pressure: ~3 x 10-3 torr
Rotational speed: 15 rpm
20
![Page 21: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/21.jpg)
20 25 30 35 40 45 50 55 60
Amorphous hump
In
tens
ity
2 Theta (degree)
Ag-based thin film
XRD identification
21
![Page 22: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/22.jpg)
Antimicrobial testStaphylococcus aureus
Sample Thickness(nm) Optical Density
Test time 0 hr 3 hr
Blank control 52.7 64.9
Ag-based TFMG (1) 53.5 65.4Ag-based TFMG (2) 1306 57.1 77.1
Ag-based TFMG (3) 1972 56.9 82.6
Ag-based TFMG (4) 1972 52.9 84.1
22
Antimicrobial test was conducted by KMUH.
![Page 23: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/23.jpg)
Antimicrobial test
24 hours 18hours12hours18 hours
Medium: Luria-Bertani (LB) broth
with bacteria with sample new medium LB agar plate23
![Page 24: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/24.jpg)
Antimicrobial testStaphylococcus aureus
Sample Thickness (nm) Colony-forming unit / plate
blank control 230Ag-based TFMG (1) 56Ag-based TFMG (2) 1306 80Ag-based TFMG (3) 1972 69Ag-based TFMG (4) 1972 49
24
Antimicrobial test was conducted by KMUH.
![Page 25: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/25.jpg)
BiocompatibilityMTT assay
Zhou et al., Mater. Sci. Eng. A., 398, 28-36 (2005)
25
![Page 26: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/26.jpg)
Motivation
1. Ti–Ta alloys exhibit good wear resistance, excellent corrosion resistance and biocompatibility. Hence, it is beneficial to enhance the surface conditions of stainless steel in biomedical implant by Ti-Ta thin films coating.
26
![Page 27: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/27.jpg)
316L stainless steel
Mechanical polished Electropolished
AFM
Sputtering
AFM Nanoindenterα-step XRD
Biological assay
MTT assay
SEM EDS
SEM
Contact angle
27
Flow chart Glass
![Page 28: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/28.jpg)
MTT assay
Conditions:• Cell: D1 bone marrow stem cell• Medium content: bone medium• Low glucose DMEM + 1.5 g sodium bicarbonate + 1% NEAA + 1% Vitamin C + 10% FBS + 1% P/S• Sample: TiTaSiZr TFMGs (about 1 cm × 1 cm) in 24 well• MTT assay: 24 hours
MTT assay was conducted by KMUH.
28
316L TiZrTaSi0.00
20.00
40.00
60.00
80.00
100.00
120.00 Metal
Cel
l via
bilit
y (%
)
316L TiZrTaSi0.00
20.00
40.00
60.00
80.00
100.00
120.00
140.00 Metal and Medium
Cel
l via
bilit
y (%
)
![Page 29: Department of Materials and Optoelectronic Science, National Sun Yat-Sen University (NSYSU)](https://reader035.vdocument.in/reader035/viewer/2022062315/5681651c550346895dd799bf/html5/thumbnails/29.jpg)
Thanks for your attention!
29