department of pharmacy 2014 · department of pharmacy 2014 2 compendium of research contents sr. no...

Department of Pharmacy 2014

1 Compendium of Research

Message of Principal

I am pleased to present third annual research compendium of Department of Pharmacy

which a compilation of research and development activities conducted in a calendar year.

This compendium comprises of detailed information of the publication, ongoing and

completed research projects, number of workshops and seminars conducted and attendant

by the students and the faculties.

In continuing the dedication of the researchers and the research scholars, Department of

pharmacy is aiming to set a matured research institution where our students will get

excellent training so that they can compete in the field with other competitors. Pharmacy

field is now becoming more research oriented which needs to facilitate the department

with sophisticated instruments. The department has been in the procedure of creating the

modern research facilities and expecting the industrial projects. The Department has able

to get some of industrial projects in collaboration which enhances the confidence of our

researchers and research scholars.

This is the third research annual compendium which reflects our continuous strength and

hard work showing inclination of the students and the faculty towards the research in

various fields of pharmacy. In order to enhance the research environment, our university

has framed the research incentive scheme. The enthusiasm of our dedicated faculties has

been to encourage our students to do the high impact research projects. I assure that this

encouragement and commitment of the faculties of the department will be continued in the

time to come. I would like to compliment all the researchers and the researcher scholars

who have contributed their knowledge and the novel research ideas at the department. I

also congratulate the compilation team to bring this compendium as the ready reference of

our dedication towards research and development.

Dr. A K Seth

Principal / HOD,

Department of Pharmacy,

Sumandeep Vidyapeeth,

Piparia, Vadodara



Contents

Sr. no Title Page no. 1 Research Policy 2 Grant / Incentive received

for research

3 Ongoing research projects 4 Completed research projects 5 Published papers



Research Policy

Introduction

This policy establishes the research environment within which academic staff and postgraduate

research students carry out their research. It also provides an overarching framework for the

development and implementation of all research management at Sumandeep Vidyapeeth

Objectives

To develop a proactive mechanism for smooth implementation of research projects.

To promote research culture among the staffs and students of the university.

Simplification of procedures like sanctions/purchases for research projects and other aspect.

To motivate researchers to apply for external funding agency.

To promote the publication from all research projects.

Practices

Central research committee circulates the notification regarding inviting research projects

from the students and staff of each constituent unit of university twice in a year.

Interested candidates write a research proposal in the prescribed format provided by central

research committee and submit it to central research committee in given time limit.

Research scholar can also apply for the research fellowship/ financial assistance / grant to

university / External agency as per procedure guideline given by central research committee.

Central research committee reviews the research projects thoroughly scrutinizes the

submitted projects, identifies the need of research projects & thrust areas of research project

submitted by research student/faculty/scholar of the respective department.

The research projects which fulfill the entire criterion and satisfy the research thrust

possessing will be considered for funding.

The Central research committee will call external experts for reviewing of projects.

The principal investigator has to obtain the SVIEC permission before commencement of the

research work.

The Principal investigator should commence his/her project at the pre-decided date and

complete in a stipulated time period.

The Principal investigator must follow the SV instrument purchase procedure for any

procurement of instrument/equipment.



Minimum one fourth grant will be disbursed at the initial stage and subsequently further

installments will be disbursed on submission of utilization certificate along with bills and

account statement of the previous grant.

The First installment will be disbursed with a written application for grant with SVIEC letter

to Director Research Cell.

All communication related to grant utilization or withdrawal of installments shall route

through Director, Research Cell.

Any patent generated by research work under this scheme will be shared with Sumandeep

Vidyapeeth.

In all publications, it is mandatory to include the name of Sumandeep Vidyapeeth.



Grant / Incentive received for research

Sr. no.

Name of Faculty Category for grant/incentive received Amount in Rs

1. Mr. Ujjwal Sahoo Paper publication 7,500/-

2. Dr. Yogesh Yadav Book chapter publication 2,500/-

3. Mr Rajesh Maheshwari

Paper publication 25,000/-

4. Mr. Ghanshyam Parmar

Paper publication 7500/-

5. Mr Nirmal Shah Paper publication 5,000/-


Paper presentation at 46th Annual conference of Indian Pharmacological society, NIMHANS convention centre, Bangalore

5,000/-

7. Dr. Balaraman

Oration Award at 46th Annual conference of Indian Pharmacological society, NIMHANS convention centre, Bangalore o

5,000/-

8. Mr. Sachin Chauhan

Paper presentation at International

conference: NIPICON - 2014 ,Dept of

Pharmacy, Nirma University, Ahmedabad

5,000/-

9. Mr. Nirmal Shah

Paper presentation at International

conference: NIPICON - 2014 ,Dept of

Pharmacy, Nirma University, Ahmedabad

5,000/-

10. Mr. Ujjwal Sahoo

Paper presentation at NIPICON - 2014 , Dept of Pharmacy, Nirma University, Ahmedabad

5,000/-

11. Dr Vikas Chandrakar

Paper presentation at APTICON – 2014, Balewadi sport complex, Pune

5,000/-

12. Mr Ashim Sen


5,000/-



5,000/-



Ongoing research projects

Students research projects

Sr no

Name of student

Title of the project

1. Roshni Gupte

Development and prospective process validation for manufacturing process of aceclofenac tablet

2. Anupama Sharma

Effect of coenzyme Q10 alone and its combination with Pentoxifylline in cisplatin induced nephrotoxicity

3. Arpit Jariwala

Development and characterization of albuminal and conformal coating for implantable medical device

4. Dhiral Patel In vitro diffusion enhancement of Cilnidipine by nanonization

5. Jainab Patel

Formulation and evaluation of microemulsion loaded gel of naproxen or topical delivery

6. Chirag Patel Formulation and evaluation of propanalol HCl loaded hydrogel beads Faculty research projects

1. Dr. Rajesh Maheshwari

Effects of coenzyme Q10 with antidiabetic and antihyperlipidemic drugs in experimental induced diabetic complication.

2. Dr. Ujjwal Sahoo

Design synthesis and pharmacological evaluation of novel oxadiazole, triazole and thiazolidinone-4-one

3. Mr. Sachin Chauhan

Design development optimization and evaluation of nanoparticulate drug delivery system of some anticancer drug.

4. Mr. Nirmal Shah

Techniques to improve bioavailability of Selective Estrogen Receptor modulators (SERMs) for treatment of osteoporosis

5. Mr. Ashim Kumar Sen

Development and validation of new analytical methods of some group of pharmaceuticals from its bulk and pharmaceutical dosage form

6. Mrs. Dhanya Sen

Development and validation of new analytical methods of some antihypertensive and antidiabetic group of pharmaceuticals from its bulk and pharmaceutical dosage form

7. Mr. Girish Sailor

Design development optimization and evaluation of novel drug delivery system of some phytopharmaceuticals.

8. Mrs. Falguni B Tandel

Analytical method development and pharmacokinetic study of some new anti-inflammatory agents

9. Mr. Ankur Javia

Design, development and characterization of colon cancer targeting folic acid conjugated Capecitabine nanoparticles

10. Mr. Chintan Aundhia

Formulation design and development of nano carrier system for treatment of osteoporosis

11. Mr. Ghanshyam Parmar

Pharmacognostic and pharmacological standardization of some Euphorbias plants.

12. Mrs. Aarti Zanwar

Analytical method development and validation of some drugs combinations



Completed research projects

Industry sponsored research projects

Sr. No. Name of the project Industry name Funds

received (Rs)

1. In vivo effect of FcE Bik in

Anaphylaxis models of Rats

and Guinea pig

Century

Pharmaceutical

Limited, Halol --

2. Effect of Livplus (PHF) on experimentally induced hepatotoxicity in animals.

Bacfo Pharmaceutical India ltd, Noida

18000/-

3. Effect of Diabac (PHF) on experimentally induced type II diabetes in rats.


39500/-

4. Effect of Lithocare (PHF) on experimentally induced urolithiasis in rats.


19500/-

5.

Acute toxicity study & Evaluation of calcium containing formulation on serum calcium level of ovariectomized rat model

Vasu Healthcare Pvt. Ltd, Vadodara

20000/-



PG Students research projects

Sr no Name of student Title of the project

1. Vipul Vanjara Formulation and evaluation of extended release gastroretentive microspheres for anti hyperlipidemic drug

2. Gopi Solanki Formulation and evaluation of nanoparticles coated with folic acid for cancer targeting

3. Anamika Joshi Influence of formulation parameters on preparation of microspheres by emulsification solvent evaporation technique

4. Ronak Patel Study of some solubility enhancement techniques in the development of the liquid dosage form of poorly soluble drug

5. Pratik Patel Solubility enhancement of poorly water soluble drug by solid dispersion technique

6. Chinar Mehta Formulation and evaluation of omeprazole delayed release tablets

7. Shreyas Shah Formulation and evaluation of new drug delivery system for cefpodoxime proxetil

8. Himanshu Vyas Formulation and evaluation of sublingual film of ondansatron

9. Sisal Shah Process validation of paracetamol suspension 10. Ishita contractor Process validation of piroxicam capsule 20 mg BP

11. Krishna Chauhan Process validation of paracetamol tablet and diclofenac sodium 630 mg BP

12. Ruchi Mistry Process validation of tablet manufacturing process of orodispersible carbamazepine

13. Charvi Patel Process validation of Lumefantrine and Artemether tablet

14. Chirag Patel Analytical method development and validation of Zaltoprofen and paracetamol in combined dosage form

15. Ms Richa Agarwal Antidiabetic and antihyperlipidemic activity of DIABAC (polyherbal formulation) on STZ – NA induced type II diabetes in rats

16. Ms Bhagyashree Pandya

Effect of Livplus (Polyherbal formulation) on experimentally induced hepatotoxicity in animals

17. Ms Falak Dakwala Acute toxicity study and evaluation of calcium containing formulation (Maxcal - C) on serum calcium level of ovaractomized rat model

18. Sumaiya Lalat Effect of vinpocetine and sesame seed on diabetic nephroplathy in wistar rats



Patel SR, Patel JD, Patel HC, Parmar G.Formulation and evaluation of

floating microspheres of venlafaxine hydrochloride. Pharma Science Monitor.

2014;5(1).

Abstract

The aim of study was formulate and evaluate floating microspheres of highly water soluble

drug venlafaxine HCl, using cellulose acetate and eudragit RS100 polymers. The

microspheres were prepared by solvent evaporation method. The prepared microsphere

showed good drug loading capacity and floating ability. The particle size was ranged

between 50 μm to 200 μm depends on the drug polymer ratio. The SEM study revealed that

microspheres were good spherical geometry and uniform size. FTIR studies of drug loading

microspheres showed no interaction of drug and polymers. The in vitro release studies

were performed in 900 ml of 0.1N HCl for 12 h using USP XXIV dissolution apparatus.

Release studies showed that microspheres that able to release the drug in sustain manner.

Selected formulations were subjected to kinetics studies and stability studies. The release

kinetics studies showed that the release the first order diffusion control and n value obtain

from Higuchi model showed the release mechanism. Stability studies indicated that

developed microspheres were stable and retain their pharmaceutical properties at room

temperature and 40ºc/75% RH of one month.

Keywords: Venalfaxine, eudragit, dissolution



Prajapati A, Kumar S, Sen AK, Zanwar A, Seth A. Spectrophotometric

method for estimation of dabigatran etexilate in bulk and its pharmaceutical

dosage form. Pharma science monitor. 2014;5:31.

Abstract

The present study describes a simple, accurate, precise and cost effective UV-Vis

Spectrophotometric method for the estimation of Dabigatran Etexilate in bulk and it’s

pharmaceutical dosage form. Estimation of Dabigatran Etexilate was carried out in

pharmaceutical dosage form by zero order spectrophotometric method using UV

visiblespectrophotometer. The solutions were prepared in ACN. Dabigatran Etexilate

showedabsorbance maxima at 313 nm. The linearity was found in the concentration range

of 3-15 μg/ml for Dabigatran Etexilate. The slop, intercept and correlation coefficients were

found to be 0.066, 0.027 and 0.999 for Dabigatran Etexilate at 313 nm, respectively. Values

for LOD and LOQ were found to be 0.100 μg/ml and 0.303 μg/ml, respectively. Values for

precision i.e. repeatability, intraday precision and inter day precision in terms of % RSD

were found to be0.48, 0.558, 0.680 < 2.0 %. Accuracy of the method was confirmed by

recovery studies and %recovery for formulation was determined and means recovery was

found to be in the range of99.48 % – 99.70 %. The proposed method has been applied

successfully for the analysis of the drug in pure and pharmaceutical dosage form.

Keywords: UV-Vis Spectrophotometer, Method validation, Dabigatran Etexilate.



Parmar G R, Gandhi K S, Sailor G U, Chauhan S P, Seth A K.Influence of

Ethylcellulose on Dissolution Profile of Carbamazepine Cyclodextrin Complex.

International Journal of Pharmaceutical Research. 2014;6(1):43.

Abstract

Carbamazepine (CBZ), a BCS class II drugs used in treatment of trigeminal neuralgia. The

present study was carried out to prepare and characterize extended-release matrix tablets

of CBZ using ethylcellulose (EC) as a polymer and drug was solubilized by manufacturing

inclusion complex with ß-cyclodextrin (ß-CD). First, inclusion complex were prepared with

ß-CD and then, tablet were prepared with EC. The tablet were formulated by keeping the

1:3 drug: ß-CD ratio constant but varying drug: polymer ratio (1:1, 1:1.5, 1:2, 1:2.5, 1:3,

1:3.5).The in-vitro drug release study revealed that either EC preparation was able to

extend the drug release only for 24 hours. The CBZ: ß-CD complex improve the solubility

and thus improve the dissolution profile of CBZ. These results suggested that the tablet

formulated using EC as a drug retarding polymers could be advantageous than

conventional CBZ tablets.

Keywords: Extended Release; Carbamazapine; ß-cyclodextrin; Ethyl cellulose; Inclusion

complex



Jaymin DP, Seth A, Nirmal S, Sachin C, Chintan A, Ankur J, et al.Preparation

and characterization of acyclovir nanosuspension. Pharma Science Monitor.

2014;5(1).

Abstract

Nanosuspension contains submicron colloidal dispersion of pharmaceutical active

ingredient particles in a liquid phase stabilized by surfactants. The poor water solubility of

drugs is major problem for drug formulation. The reduction of drug particles into the sub-

micron range leads to significant increase in the dissolution rate, bioavailability as well as

improve stability. Nanosuspension consists of the pure poorly water-soluble drug without

any matrix material suspended in dispersion. Nanosuspension manyattempts have been

made to deliver poorly water soluble drugs as a nanosuspension prepared by adopting

various methods. Techniques such as media milling and homogenization have been used

commercially for producing nanosuspension. Recently, the engineering of nanosuspension

employing emulsions and microemulsion as templates. The unique features of

nanosuspension have enabled their use in various dosage forms, including specialized

delivery systems such as mucoadhesive hydrogels, parenteral, per oral, ocular and

pulmonary routes.

Keywords: Nanosuspension, Solubility enhancement, Saturation solubility,

Homogenization



Maheshwari R, Balaraman R, Sailor G, Parmar G, Patel A, Seth A. Antiulcer

and antioxidant effects of Normacid syrup (a polyherbal formulation) on

experimentally-induced gastric ulcers. Oriental Pharmacy and Experimental

Medicine. 2014;14(2):145-55.

Abstract

The aim of this work was to study the antioxidant and antiulcer activities of Normacid

syrup® (NS) in mice. Effects of NS (250 and 500 mg/kg, p.o., for 14 days) were studied on

pylorus ligation and diclofenac-induced ulcers. Antiulcer activity of NS was assessed from

gastric secretion parameters, mucosal nitrite level and mucin content in gastric mucosa.

The activity of antioxidant enzymes like super oxide dismutase (SOD), catalase (CAT),

reduced glutathione (GSH) and lipid peroxidation (MDA) in the stomach tissue were

quantified. Histopathological studies were done on stomach tissues. Pre-treatment with NS

significantly (p<0.05) reduced ulcer score and ulcer index in pylorus ligated (62.20–67.50

% protection) and diclofenac (64.19–70.85 % protection)-induced gastric ulcers. NS

decreased the gastric volume, free acidity and total acidity and increased the pH of gastric

fluid. Simultaneously, the level mucosal nitrite and mucin content were increased

significantly (p<0.001). In addition, pre-treatment with NS significantly increased activities

of SOD (p<0.001), CAT (p<0.001), GSH (p<0.05 to p<0.001), and reduced the MDA level

(p<0.001), in both models. The antiulcer activity of NS is further supported by attenuation

of histopathological changes caused by pylorus ligation and diclofenac. The results

suggested that NS has potential antiulcer activity against pylorus ligation and diclofenac-

induced ulcer model. The antiulcer activity might be due to its antisecretory, cytoprotective

and antioxidant mechanism.

Keywords: Pylorus ligation Diclofenac Ayurvedic formulation Antiulcer



Maheshwari RA, Balaraman R, Sailor GU, Parmar GR, Patel M, Seth A.

Antianaphylactic, mast cell stabilizing and antiasthmatic activity of AHR-1 (a

polyherbal formulation). Pharmacognosy journal. 2014;6(3):93.

Abstract

Objective: This work was mainly aimed to study the anti-anaphylactic, mast cell stabilizing

and antiasthmatic activity of AHR-1 (a polyherbal formulation) which contain various

herbal extracts. Methods: The antianaphylactic activity of AHR-1 was evaluated in rats

using active anaphylaxis model. Rats were then observed for onset of symptoms of

anaphylaxis reaction such as increased respiratory rate, dyspnea, cyanosis and mortality.

Serum IgE, leukocyte, eosinophil countand % polymorphs were calculated. Mast cell

stabilizing effect was investigated by in vitro challenge of antigen sensitized rat intestinal

mesenteries. Antiasthmatic effect was studied in guinea pigs using histamine-induced

bronchospasm, in which occurrence of preconvulsive dyspnea (PCD) was noted as end

point. Results: Anaphylactic shock caused by intravenous antigen challenge showed 83%

mortality with a significant (P<0.001) increase respiratory symptom score. Treatment with

AHR-1(250 and 500 mg/kg) reduced the mortality and respiratory symptom score (P<0.05,

P<0.001), respectively. AHR- 1 (250 and 500 mg/kg) significantly and dose dependently

decreased Serum IgE (P<0.05, P<0.001), AEC (P<0.05, P<0.001), total leukocytes (P<0.05,

P<0.01) and % polymorphs (P<0.01,P<0.001), respectively as compared to sensitized

control group. Sensitized control rats were produced a significant (79%) mesenteric mast

cell degranulation, but pre-treatment with AHR-1 (100 and 200μg/ml) produced in a

significant (p<0.001) reduction in the number of degranulated mast cells when challenged

with horse serum. AHR-1 significantly increased the time of PCD (P<0.001) as compared to

control. Conclusion: From these finding, we concluded thatAHR-1 is might be effective in

treatment various hypersensitivity reactions like anaphylactic shock and asthma.

Keywords: Horse serum, AHR-1, IgE, Respiratory score



Maheshwari RA, Balaraman R, Sen AK, Seth A. Effect of coenzyme Q10

alone and its combination with metformin on streptozotocin-nicotinamide-

induced diabetic nephropathy in rats. Indian journal of pharmacology.

2014;46(6):627.

Abstract

Objectives: This study was aimed to investigate the therapeutic potential of coenzyme Q10

and its combination with metformin on streptozotocin (STZ)-nicotinamide-induced

diabetic nephropathy(DN).Materials and Methods: Type 2 diabetes in rats was induced

with STZ-nicotinamide. The diabetic rats were treated with coenzyme Q10 (10 mg/kg, p.o.)

alone or coenzyme Q10 + metformin. Various parameters of renal function tests such as

serum creatinine, urea, uric acid, and markers of oxidative stress such as renal

malondialdehyde (MDA) level, superoxide dismutase (SOD), and catalase (CAT) activities

were measured. Tumor necrosis factor-α (TNF-α), myeloperoxidase (MPO) activity,

transforming growth factor-β (TGF-β), and nitrite content were estimated in renal tissues.

All treated animal were subjected to histopathological changes ofkidney. Result: Diabetic

rats showed a significant reduction in renal function, which was reflected with an increase

in serum urea, serum creatinine, uric acid. In addition, STZ-nicotinamide caused renal

tubular damage with a higher MDA level, depletion of SOD and CAT activity and glutathione

(GSH) level. Moreover, TNF-α, MPO activity, TGF-β, and nitrite content were significantly

increased in diabetic rats, while treatment with coenzyme Q10 or metformin or their

combination ameliorate STZ-nicotinamide induced renal damage due to improvement in

renal function, oxidative stress, suppression of TNF-α, MPO activity, TGF-β and nitrite

content along with histopathological changes. Conclusions: This finding suggests that the

treatment with coenzyme Q10 or metformin showed significant renoprotective effect

against STZ-nicotinamide-induced DN. However, concomitant administration of both

showed a better renoprotective effect than coenzyme Q10 or metformin alone treatment.

Keywords: Coenzyme Q10, diabetic nephropathy, metformin, transforming growth factor-

β, tumor necrosis factor-α



Maheshwari RA, Khatri K, Sailor G, Balaraman R. Antidiabetic activity of

Dibolin (a polyherbal formulation) in streptozotocin-nicotinamide induced

type 2 diabetic rats. Int J Pharm Pharm Sci. 2014;2:893-7.

Abstract

Objective: The objective of the study was aimed to investigate the antidiabetic activity of

Dibolin (a polyherbal formulation) in streptozotocin – nicotinamide induced type 2 diabetic

rats. Methods: Oral glucose tolerance test (OGTT) was performed to evaluate Dibolin effect

on elevated glucose level. The type 2 diabetes was induced by overnight fasted rats by a

single intraperit one al (i.p.) injection of 65 mg/kg streptozotocin, 15 min. after the i.p.

administration of 110 mg/kg nicotinamide. The diabetic rats were treated with Dibolin

(500 and 1000 mg/kg, p.o.) or glibenclamide (5 mg/kg, p.o) for four week. Various

parameters were studied included fasting blood sugar level, serum insulin levels, glycated

hemoglobin (HbA 1C), serum lipid levels, AST, ALT, serum creatinine, urea, uric acid. Result

s: Treatment with Dibolin significantly reduced blood sugar levels in OGTT. Diabetic rats

showed a significant increase in the levels of glycated hemoglobin, serum lipids, AST, ALT,

serum creatinine, urea and uric acid, whereas there was a significant decrease in Hb, serum

insulin and HDL – C levels as compared to normal control rats. The administration of

Dibolin or glibenclamide significantly decreased the levels of glycated hemoglobin, TG, TC,

LDL - C, AST, ALT, serum creatinine, urea and uric acid, whereas there was a significant

increase in the level of Hb, serum insulin and HDL - C as compared to diabetic control rats.

Conclusion: These results concluded that Dibolin caused antidiabetic and

antihyperlipidemic activities which are responsible for its use in traditional medicine.

Keywords: Streptozotocin,Dibolin,glycated hemoglobin, serum lipids



Sahoo D, Gosai H, Sahoo U, Akhani P, Kanchan A, Harsoda J. Assessment of

daytime sleepiness and reliability test of Epworth sleepiness scale in young

individuals. Tanta Medical Journal. 2014;42(2):79.

Abstract

Background: The Epworth Sleepiness Scale (ESS) is a brief, self-administered

questionnaire to measure the daytime sleepiness (DS) in young individuals. It measures an

individual's usual level of DS or average sleep propensity in daily life. The scale also

estimates whether someone is experiencing excessive sleepiness, which possibly requires

medical attention. Aim: The aim of the study was to assess the level of DS as well as to

assess the reliability of ESS in young individuals. Settings and design: The study was

designed as a cross-sectional questionnaire-based study. Participants and methods: A

total of 120 apparently healthy participants belonging to both sexes were evaluated at

baseline and after 6 months. The anthropometric measurements included BMI and neck

width, ESS scores, DS by the mean levels of chance of dozing, O 2 saturation, and pulse rate.

Statistical analysis: The reliability of ESS scores and its correlations with various

parameters were assessed using paired T-test. Results: The mean levels of chance of

dozing and ESS scores were significantly lower during daytime in female individuals. ESS

scores were significantly correlated with BMI, neck width, and pulse rate; however, there

was no correlation between ESS scores and O 2 saturation. The ESS scores as well as the

above-mentioned results did not change significantly even after 6 months, indicating good

reliability. Conclusion: The ESS is appropriate for measuring DS in young individuals, and

it also enables characterization of individuals with sleep-related disorders.

Keywords: chance of dozing, daytime sleepiness, Epworth Sleepiness Scale, O 2 saturation,

reliability



Sahoo D, Gosai H, Sahoo U, Harsoda J. Awareness and Practices of Non-

Pharmacological Approaches for Management of Hypertension in a Geriatric

Population. International Journal of Medical Students. 2014;2(2):53-5.

Abstract

Background: There is an increase in the prevalence of hypertension all over the world,

including India. Hypertension can be initially managed with non-pharmacological

measures. This study aims to assess the knowledge of non-pharmacological measures to

control hypertension and its application in a geriatric hypertensive population. Methods:

The study was conducted at the Department of Physiology, SVU, Vadodara, India. A total

110 hypertensive patients were included in the study and a non-validated survey was

conducted to examine knowledge of non-pharmacological measures to control

hypertension in this group of patients. Frequencies, percentages, means and standard

deviations were calculated and reported. Results: Only 10% of the respondents knew the

normal values for blood pressure. Approximately 38% of the subjects did not measure their

blood pressure regularly. A total of 24% subjects knew that body weight has a correlation

with hypertension. About 27% said that there was no correlation between salt intake and

hypertension, and 88% of the study population did not carry out any form of physical

activity. Conclusion: Hypertension can be controlled by life style modifications such as

exercise, weight management and a healthy diet. Public health and education measures

targeting hypertensive population need to be taken to decrease the risk factors for

cardiovascular diseases and, therefore, improve people's health and quality of life.

Keywords: Hypertension; Health Knowledge, Attitudes, Practice; Exercise; Geriatric

Assessment (Source: MeSH-NLM).



Patel CD, Sen AK, Sen DB, Sahoo U, Seth A. Analytical method development

and validation of uv spectroscopic method for simultaneous estimation of

zaltoprofen and paracetamol in combined dosage form. Pharma Science

Monitor. 2014;5(3):59-70.

Abstract

A simple, accurate, precise & sensitive UV spectroscopic method was developed for the

simultaneous determination of zaltoprofen (ZLT) & paracetamol (PCM) in combined tablet

dosage form. For the simultaneous equation method, the wavelength range of both the

drugs zaltoprofen & paracetamol were selected as 227 nm and 243 nm. The linearity was

found in the concentration range of 1 to 5 μg/ml and 4.062 to 20.31 μg/ml for zaltoprofen

and paracetamol, respectively. The slope, intercept, correlation coefficient values of

zaltoprofen & paracetamol at 227 nm & 243 nm were found to be 0.032, 0.047, 0.999 (227

nm); 0.029, 0.043, 0.998 (243 nm) & 0.041, 0.073, 0.999 (227 nm); 0.059, 0.095, 0.999 (243

nm). The % recovery values were found to be 98.66 % - 99.09 % for zaltoprofen and 98.40

% - 98.86% for paracetamol, stated that the method was accurate. The LOD and LOQ values

of both the drugs zaltoprofen & paracetamol were found to be 0.216 μg/ml, 0.656 μg/ml

(227 nm); 0.238 μg/ml, 0.724 μg/ml (243 nm) & 0.225 μg/ml, 0.682 μg/ml (227 nm);

0.117 μg/ml, 0.355 μg/ml (243 nm). This method was successfully applied to the

determination of zaltoprofen & paracetamol content in marketed formulation. The method

was validated statistically as per ICH guideline. The results proved that the method can be

employed for the routine analysis of zaltoprofen & paracetamol in the combined

formulations.

Keywords: Simultaneous equation method, UV-Visible spectrophotometer, Zaltoprofen,

Paracetamol.



Zanwar AS, Sen DB, Ruikar DB, Seth A. Spectroscopic methods for the

simultaneous estimation of mometasone furoate and formoterol fumarate in

rotacaps. Indo American Journal of Pharmaceutical Science. 2014;4(12):5928-

32.

Abstract

Two spectrophotometric methods Zero crossing derivative and ratio- spectra derivative

spectrophotometric methods were developed for the determination of Mometasone

furoate (MF) and Formoterol fumarate (FF) in the pharmaceutical dosage form. Zero

crossing dervative spectrophotometry involves amplitudes measurement of the first

derivative spectra of the standard and sample solution at 267.56 nm for MF and 306 nm

for FF. Ratio spectra derivative spectrophotometry involves amplitudes measurement of

the ratio first derivative spectra at 263nm and 267.30 nm for MF and 293.08 and 302.60

nm for FF. The calibration graph follows beer’s law in the range of 30-200 µg /ml of MF and

2-6 µg /ml for FF. The accuracy and precision of the method were validated statistically. So

it can be a preferable method for routine analysis due to its simplicity and economical

advantages.



Sahoo D, Gosai H, Shah Y, Sahoo U, Harsoda J. A Screening Study on

Prevalence of Anemia in Pregnant Women during Different Trimester.

Scholars Journal of Applied Medical Sciences. 2014; 2(5): 1639-1642.

Abstract

Maternal nutrition is very essential determinant influence during the development of

foetus. Inadequate intake or absorption of iron in conjunction with blood loss during

pregnancy may contribute to anemia. Anemia is the most common nutritional deficiency

disorder in the world. The aim of the study was to complete blood count in 2nd and 3rd

trimester during pregnancy and find out prevalence of anemia in these subjects. Pregnant

women who had hemoglobin (Hb) value of < 11gm were selected. Total 80 anemic

pregnant women were enrolled for the study. Out of 80, 28 (35%) belongs to primigravida

and 52 (65%) multigravida. Among 80 participants, 54.54% were mild anemic, 43.92%

were moderate and 3.54% were severe anemic. Other hematological parameters like RBC

(Red blood cell) count, HCT (Hematocrit), MCV (Mean corpuscular volume), MCHC (Mean

corpuscular hemoglobin concentration) and MCH (Mean corpuscular hemoglobin) were

also below the normal range. There is a need to monitor hemoglobin during pregnancy

and there by improve the outcome of pregnancy.

Keywords: Pregnancy, Trimester, Hemoglobin, Prevalence, Anemia, Gravida



Sahoo U, Seth A, Balaraman R. Synthesis of Some Novel 1, 2, 4 - Triazole

Derivatives Bearing Benzimidazole Nucleus and Biological Evaluation of Their

Possible In Vitro Anti Inflammatory and Antioxidant Activity. Research

Journal of Pharmaceutical, Biological and Chemical Sciences. 2014;5(6): 246-

253.

Abstract

Series of 4-[1-({5-[3-(substituted) phenyl]-4H-1, 2, 4-triazol-3-yl} methyl)-5-substituted -

1H-benzimidazol-2-yl] benzonitrile 8(I-XXXI) were synthesized. Substituted o-

phenylenediamine was reacted with substituted 4-cyanobenzaldehydes in the presence of

sodium metabisulfite to furnish substituted 2-(4-Cyanophenyl)-1H-benzimidazoles (1).

When these substituted 2-(4-Cyanophenyl)-1H-benzimidazoles were further treated with

ethyl chloroacetate in KOH/DMSO, N-alkylated product(2-(4-cyanophenyl)-benzimidazol-

1-yl) - acetic acid ethyl esters (2) was formed. To synthesize 2-(4-cyanophenyl)-

benzimidazol -1-yl)-acetic acid hydrazides (3)chemical reactions were conductedbetween

Hydrazine hydrate and the esters (2). The structures of newly synthesized compounds 8(I-

XXXI) was confirmed by suitable spectroscopic techniques such as IR, 1H NMR, 13C NMR

and m/z ratio. All the synthesized compounds were screened for its in vitro anti-oxidant

and anti -inflammatory activity. The in-vitro anti-oxidant and anti-inflammatory activity

might be attributed due to the presence of more electrons withdrawing group and moiety

having more lipophilicity also more electro negativity in nature.

Keywords: O- phenylenediamine, 4-cyanobenzaldehyde, 1, 2, 4-triazole, Antioxidant, Anti -

inflammatory



Ujjwal S, Seth A, Balaraman R. Design, Synthesis of some Novel 1, 3, 4-

Oxadiazole derivatives bearing Benzimidazole Nucleus and Biological

evaluation of their possible in-vitro Anti-inflammatory and Antioxidant

activity. International Journal of Chem Tech Research. 2014;6(4):2427-2437.

Abstract

Some novel 1,3,4-oxdiazole derivatives bearing benzimidazole nucleus were synthesized

and their in-vitro anti-inflammatory and antioxidant activity by inhibition of protein

denaturation screening, 2, 2-Diphenyl-1-Picryl Hydrazide screening methods respectively.

The compound 7.XX and 7.XXIX were found to be highly active in low concentration and

compounds 7.VIII, 7.IX, 7.X, 7.XIX, 7.XXII, 7. XXIV and 7.XXV were found to be moderately

active at higher concentration as compared to ascorbic acid in 2, 2-diphenyl-1-picryl hydra

zide method. In-vitro anti-inflammatory by inhibition of protein denaturation method the

compounds 7.VIII, 7.XX, 7.XXII and 7.XXIX were found to be highly active in low

concentration and compounds 7.IX, 7.X, 7.XIX, 7. XXIV, 7.XXV and 7.XXXIII were found to be

moderately active at higher concentration as compared to diclofenac sodium.

Keywords: O-phenylenediamine, 4-cyanobenzaldehyde, imines intermediate, 1,3,4-

oxadiazole.



R. Chawla, A. Kaura, A. Arora, V. Garg, R. Sharma, U. Sahoo, et al.

Antioxidant and antibacterial studies of thiazolidin-4-ones containing benzo

[b] thiophene moiety. Pharma Science Monitor. 2014;5(1): 226-238.

Abstract

A novel series of 2-(substituted phenyl)-3-(3-chloro-1-benzo[b]thiophene-2-carboxamido)-

-thiazolidinones and 2-(substituted phenyl)-3-(3-chloro-1-benzo[b]thiophene-2-

carboxamido)-5-carboxymethyl-4-thiazolidinones have been synthesized and evaluated for

antimicrobial and antioxidant activities. Initially, 3-chloro-1-benzo[b]thiophene-2-carbonyl

chloride (1) was prepared from cinnamic acid in the presence of chlorobenzene and thionyl

chloride. This compound (1) was treated with hydrazine hydrate to afford 3-chloro-1-

benzo[b]thiophene-2-carbohydrazide [2] which was further reacted with various aromatic

aldehydes to yield hydrazones 2(a-h). Further reaction of these hydrazones 2a-h with

thioglycollic acid gave 2-(substituted phenyl)-3-(3-chloro-1-benzo[b]thiophene-2-

carboxamido)-4-thiazolidinones 3a-h. Reaction of the same compounds 2a-h in the

presence of thiomalic acid afforded 2-(substituted phenyl)-3-(3-chloro-1-

benzo[b]thiophene-2-carboxamido)-5-carboxymethyl-4-thiazolidinone 4a-h. The tructures

of newly synthesized compounds 3a-h and 4a-h have been confirmed by spectroscopic

techniques such IR, 1H NMR, 13C NMR and elemental analysis. The synthesized compounds

were screened for their antimicrobial and antioxidant activities. Compounds 3d and 4a-h

showed significant antibacterial activity and compounds 3d, 3h and 4h were found to be

the most active antioxidants in the series, and thus represent a new class of promising lead

compounds.

Keywords: Antioxidant, Antibacterial, Thiazolidin-4-one, Benzo [b] thiophene.



Debalina Sahoo, Harshida Gosai, Ujjwal Sahoo, JM Harsoda. Non-

Pharmacological Measures For Control of Hypertension in Geriatric

Population. Journal of Bangladesh Society of Physiologist. 2014;9(2): 94-97.

Abstract

Background: Increased prevalence of hypertension in all over the world is well

recognized. Hypertension affects almost all body organs and systems. It can be managed by

using nonpharmacological measures as well. Objective: To assess knowledge of non-

pharmacological measures to control of hypertension and its application in geriatric

hypertensive population. Methods: The study was conducted in the Department of

Physiology, Sumandeep Vidyapeeth University, Vadodara. Total 108 hypertensive patients

had been included for this study. Subjects were administered pre tested ten questionnaires.

They responded the questionnaires at the same time, without any discussion. Results:

Knowledge about normal level of blood pressure was lacking in 70% of study population.

Conclusion: Knowledge about weight reduction, regular exercise, fruits and vegetables

intake was poor. About half of the study population said that blood pressure can be

controlled by using of drugs only. So, they were not aware of non-pharmacological

measures.

Keywords: Hypertension; knowledge; exercise; geriatric; population



Nirmal Shah, Avinash Seth, Sachin Chauhan, Chintan Aundhia, Ankur

Javia, Girish Sailor. Formulation, design and characterization of

Microemulsion based system for topical delivery of Antipsoriatic drug. World

journal of pharmacy and pharmaceutical sciences. 2014;3(2): 1464-1480.

Abstract

The purpose of this study was to develop a stable methotrexate (MTX) loaded

microemulsion gel (MMG) for topical use in psoriasis to improve cutaneous deposition and

local effect. The pseudo-ternary phase diagrams were developed for various

microemulsion formulations composed of Capmul MCM - C8 as oil phase, Tween 20 as

surfactant and polyethylene glycol 400 (PEG 400 as cosurfactant. Composition of

microemulsion system was optimized using concentration of oil, surfactant/cosurfactant

(1:1) and water as independent variables. The MTX- loaded microemulsion was

characterized by droplet size and zeta potential. Microemulsion gel was prepared by

adding 1% Carbopol 934 as a gelling agent. The transdermal ability of MTX from

microemulsion gel was evaluated by in vitro permeation study. The results shows that

optimized microemulsion formulation was composed Capmul MCM - C8 (7.5% w/w),

Tween 20 (37.5% w/w), PEG 400 (12.5% w/w) and water (42.5% w/w). The optimized

microemulsion was found to be relatively uniform in size of optimized (11.52 ± 0.6 nm).

The MMG showed enhanced in vitro permeation ability with better drug deposition

capacity compared MTX solution, gel and microemulsion. The results suggest that the MMG

is promising formulation for topical delivery of MTX for psoriasis treatment.

Keywords: Methotrexate; Microemulsion; Microemulsion gel; Topical delivery;

Antipsoriatic



Shah N, Patel K, Seth A, Chauhan S, Javia A, Patel J. Design & evaluation of

controlled release floating microspheres for better management of

hypertension. Pharma science monitor. 2014;5(3): 134-144.

Abstract

The objective of the present work was to formulate and evaluate microspheres dosage

form with Ramipril as the model drug. Ramipril is ideally suited to be formulated in an

extended release drug delivery system to improve its patient compliance by bringing down

its frequency of administration. Microspheres were prepared by non-aqueous

emulsification-solvent evaporation technique using different combination of Ethyl cellulose

and/or Eudragit L100 a polymers. The major advantage of the preparation technique

includes short processing time, ambient temperature processing, and high encapsulation

efficiency along with being economical. The mixing ratio of components in the organic

phase affected the size distribution, drug content, percentage yield and release profile of

microspheres. Best results were obtained at the ratio of drug: polymer (1:1). The

microspheres formed were additionally found to be floating over gastric juice for > 8 hours.

The developed Floating microspheres of Ramipril might be clinically used for prolonged

drug release in GIT, for better drug utilization and improved patient compliance.

Keywords: Hypertension; entrapment efficiency; floating time; microspheres; Solvent

evaporation

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