dept spectra cosy spectra hetcor spectra. dept-90, dept-135 distortionless enhancement by...
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DEPT SpectraCOSY Spectra
HETCOR Spectra
DEPT-90, DEPT-135Distortionless Enhancement by Polarization
Transfer• Preferred procedure for determining # protons
attached to carbons• Variable proton pulse angle is set at 90o and
135o
• In DEPT-90, only CH shows. In DEPT-135, CH2’s are phased down, CH and CH3 are phased up
Adjustment of 1H pulse angle
Avoids overlapping multiplets CH
CH2
CH3
2-Heptanone, CPD and DEPT-35
6-Methyl-5-hepten-2-olStandard CPD Spectrum
DEPT-90DEPT-135
OH
O
CH3
DEPT-135
DEPT-90
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Limonene
Ipsenol CPD, DEPT-135, DEPT 90
76
25 3
4 19
8,10
CO2CH3
CH3CO
O
135
90
Correlated Spectroscopy
FT of the t1 domain, acetone
O c t o b e r 2 0 , 2 0 0 4 J o a n n a R . L o n g 1 5
2 D E x c h a n g e N M R
A . S . E d i s o nU n i v e r s i t y o f F l o r i d a
t 1 t 2
F T o f t 2 FT in t1 w
ill give 2D frequency spectrum
October 20, 2004 Joanna R. Long 16
15 l N•Ndimethylacetamide in 700 l d-chloroform at 29° C
A-B coupling in COSY Spectrum
CH3CH2CCH3
O
CH2CH3
Isobutyl Alcohol
OH
O
O
1H NMR Spectrum of Ipsenol
COSY Spectra of Ipsenol
HO
1
23
4
5 6 7
8
9
HETCOR Spctrum of Ipsenol
N
COCH2CH3
O
H
H
H
H
Exercise 5.6, p. 291
N
COCH2CH3
O
H
H
H
H
Exercise 5.6, p. 292
O
CH3