development of a thermal cooling system for a pmt (photo multiplier tube) summer researcher yasmine...
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![Page 1: Development of a thermal cooling system for a PMT (Photo Multiplier Tube) Summer Researcher Yasmine Salas Colorado State University Advisor: Dr. Julia](https://reader036.vdocument.in/reader036/viewer/2022062714/56649d4d5503460f94a2ce41/html5/thumbnails/1.jpg)
Development of a thermal cooling system
for a PMT(Photo Multiplier Tube)
Development of a thermal cooling system
for a PMT(Photo Multiplier Tube)
Summer Researcher Yasmine SalasColorado State University
Advisor: Dr. Julia LyobuviksyBio-Engineering Department
University of California, Riverside
Summer Researcher Yasmine SalasColorado State University
Advisor: Dr. Julia LyobuviksyBio-Engineering Department
University of California, Riverside
![Page 2: Development of a thermal cooling system for a PMT (Photo Multiplier Tube) Summer Researcher Yasmine Salas Colorado State University Advisor: Dr. Julia](https://reader036.vdocument.in/reader036/viewer/2022062714/56649d4d5503460f94a2ce41/html5/thumbnails/2.jpg)
OutlineOutline
Background of the Multi Photon laser microscopy
Objective How PMT works and why a cooling
system is needed Conclusion Acknowledgements
Background of the Multi Photon laser microscopy
Objective How PMT works and why a cooling
system is needed Conclusion Acknowledgements
![Page 3: Development of a thermal cooling system for a PMT (Photo Multiplier Tube) Summer Researcher Yasmine Salas Colorado State University Advisor: Dr. Julia](https://reader036.vdocument.in/reader036/viewer/2022062714/56649d4d5503460f94a2ce41/html5/thumbnails/3.jpg)
ObjectiveObjective
To develop a cooling system for a PMT (Photo Multiplier Tube)
Engineer effective cooling between 5C-35C for Optimum performance.
Configure prototypes models using AutoCAD
Create an efficient holding container for cooling substance whether liquid nitrogen or ethanol, dry ice mixture.
To develop a cooling system for a PMT (Photo Multiplier Tube)
Engineer effective cooling between 5C-35C for Optimum performance.
Configure prototypes models using AutoCAD
Create an efficient holding container for cooling substance whether liquid nitrogen or ethanol, dry ice mixture.
![Page 4: Development of a thermal cooling system for a PMT (Photo Multiplier Tube) Summer Researcher Yasmine Salas Colorado State University Advisor: Dr. Julia](https://reader036.vdocument.in/reader036/viewer/2022062714/56649d4d5503460f94a2ce41/html5/thumbnails/4.jpg)
Why use this microscope Why use this microscope
Two-photon excitation microscopy is a fluorescence imaging technique that allows imaging living tissue up to a depth of one millimeter.
The two-photon excitation microscope is a special variant of the multiphoton fluorescence microscope. Two-photon excitation may in some cases be a viable alternative to confocal microscopy due to its deeper tissue penetration and reduced phototoxicity.
Two-photon excitation microscopy is a fluorescence imaging technique that allows imaging living tissue up to a depth of one millimeter.
The two-photon excitation microscope is a special variant of the multiphoton fluorescence microscope. Two-photon excitation may in some cases be a viable alternative to confocal microscopy due to its deeper tissue penetration and reduced phototoxicity.
![Page 5: Development of a thermal cooling system for a PMT (Photo Multiplier Tube) Summer Researcher Yasmine Salas Colorado State University Advisor: Dr. Julia](https://reader036.vdocument.in/reader036/viewer/2022062714/56649d4d5503460f94a2ce41/html5/thumbnails/5.jpg)
Multiphoton Microscopy (MPM)-Non-linear optical technique
Z
z
xy
Ti-Sap Laser
scanning
mirrors
spectrograph
CCD
PMT
3D image capabilityand spectroscopy
Backscattering geometry = practical utility for in vivo thick-tissue applications
• Reduced scattering at near- IR wavelengths
• No out of focus absorption
• Very small sample volume
• Non-destructive to samples
• Deeper tissue penetration
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What is a PMT?What is a PMT?
Photo multiplier tube Photomultiplier tubes (PMTs)
convert photons to an electrical signal. They have a high internal gain and are sensitive detectors for low-intensity applications such as fluorescence spectroscopy.
Photo multiplier tube Photomultiplier tubes (PMTs)
convert photons to an electrical signal. They have a high internal gain and are sensitive detectors for low-intensity applications such as fluorescence spectroscopy.
![Page 7: Development of a thermal cooling system for a PMT (Photo Multiplier Tube) Summer Researcher Yasmine Salas Colorado State University Advisor: Dr. Julia](https://reader036.vdocument.in/reader036/viewer/2022062714/56649d4d5503460f94a2ce41/html5/thumbnails/7.jpg)
How a PMT worksHow a PMT works
QuickTime™ and a decompressor
are needed to see this picture.
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Inside the PMTInside the PMT
QuickTime™ and a decompressor
are needed to see this picture.
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Drawing Board Drawing Board
Create a cooling system with aluminum to obtain desired temperature and effective insulation to direct
heat away from the PMT and into the cooling system.
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Prototype 1Prototype 1
The radiator module did not work because there was to much heat flux. Temperatures would vary depending
on area of the container.
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Prototype 2Prototype 2
This model did not work because the container would create to much pressure
within the box if liquid nitrogen was used, ethanol and dry ice mixture did not cool to
desired temperatures.
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Final ProductFinal Product
The final module allowed a constant temperature for all sides it was also insulated with a thick compacted form of Styrofoam and substituted
liquid nitrogen for a ethanol and dry ice mixture. Also a small window of contact was cut in the
Styrofoam to allow a better heat transfer with the PMT.
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ConclusionConclusion
Cooling system works efficiently cooling systems similar to the PMT while holding mixture of ethanol
and dry ice but yet has to be tested with liquid nitrogen on a
PMT, which has not yet been received.
Cooling system works efficiently cooling systems similar to the PMT while holding mixture of ethanol
and dry ice but yet has to be tested with liquid nitrogen on a
PMT, which has not yet been received.
![Page 14: Development of a thermal cooling system for a PMT (Photo Multiplier Tube) Summer Researcher Yasmine Salas Colorado State University Advisor: Dr. Julia](https://reader036.vdocument.in/reader036/viewer/2022062714/56649d4d5503460f94a2ce41/html5/thumbnails/14.jpg)
Acknowledgements Acknowledgements
Advisor Dr. Julia Lyubovitsky Dr Victor Rodgers
(Department of Bioengineering) Jun Wang
BRITE(University of California, Riverside)
Advisor Dr. Julia Lyubovitsky Dr Victor Rodgers
(Department of Bioengineering) Jun Wang
BRITE(University of California, Riverside)