Development of Bleaching Reagent Testing by Using Mangosteen Peel Extract Solution

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Members

Nathida Wiriyachoke No.2 M.4/1

Chanon Pinyopawasutti No.11 M.4/1

Nuttanon Jantanapornchai No.13 M.4/1

AdvisorDr. Usa Jeenjenkit 2

Inquiry questions• How the mangosteen peel extract

solution identify the concentration of bleaching reagent ?

• Can mangosteen peel extract identify the concentration of bleaching reagent ?

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Introduction

http://www.vcharkarn.com/varticle/40332 http://www.vcharkarn.com/varticle/42442http://fic.ifrpd.ku.ac.th/fic/index.php/th/simplelist/434-fruit-28-09-2010.html

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Bleaching reagent

Sodium Metabisulphite (Na2S2O5)

Sodium hydrosulfite (NaHSO4)

Sodium hypocloride (NaOCl)

http://www.palanadenterprises.com/sodium-metabisulphite.htm

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The benefit of bleaching reagentDying industry : Making new

colors Food industry : making food

more attractive

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Disadvantage

Frequently use of bleach would effect to the respiratory symptoms (Zock et. al., 2009).

http://www.buzzle.com/articles/lower-abdominal-pain-and-lower-back-pain.html 7

One out of 560 larvae treated with BW [bleach solution] (97.3% of the animals were uninfected) was recovered as an adult worm (Zanini & Graeff-Teixeira, 2001).

http://www.sciencephoto.com/images/download_lo_res.html?id=903410120 8

Bleach causes a chemical reaction in the gill tissue which blocks the transfer of oxygen and causes the lobster to slowly asphyxiate

( T. Wilson, Vaughan, K. Wilson,Simon & Lockhart , 2007).

http://members.cox.net/jjschnebel/lobsnewb.html 9

Scope

Bleaching reagent : sodium

metabisulphite Bleaching reagent

concentration :1, 5, 50 and 100 ppm

Dilution ratio of mangosteen extract solution :1:120, 1:160 and 1:200

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Mixture ratio of bleaching reagent and mangosteen extract : 1:2

Wavelength of spectrophotometer : 410 nm

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Materials & Method

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Prepare bleaching reagent solution.

Na2S2O5 0.1 gH2O 1 liter

Na2S2O5(aq) 100 ppm

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Dilute bleach solution

Na2S2O5(aq) 5 ml 100 ppm

H2O 5 ml

Na2S2O5(aq) 50 ppm

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Mix the extract solution and different concentration of bleaching solution by 2:1 (v/v).

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Measure the absorbance by using spectrophotometer at wavelength 410 nm.

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Results1:120 of Mangosteen peel extract : water Concentra

tion of bleach

solution %T Abs Conc.

0 ppm 0.5 N/A1147.

0

1 ppm 2.9 1.524 745.6

10 ppm 4.7 1.331 651.5

50 ppm 6.0 1.225 600.3

100 ppm 6.7 1.178 574.3

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1:160 of Mangosteen peel extract : water (First time)

Concentration

of bleach

solution %T Abs Conc.

0 ppm 0.4 N/A N/A

1 ppm 2.2 1.651 809.6

10 ppm 8.7 1.072 516.4

50 ppm 7.1 1.001 491.8100 ppm 8.4 1.072 530.3

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1:160 of Mangosteen peel extract : water ( Second time)

Concentration

of bleach solutio

n %T Abs Conc.

0 ppm 1.3 1.865 916.9

1 ppm 5.9 1.229 601.9

10 ppm 6.4 1.195 585.4

50 ppm 6.5 1.187 582.5100 ppm 6.6 1.178 577.3

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1:200 of Mangosteen peel extract : water

Concentration

of bleach solutio

n %T Abs Conc.

0 ppm 3.4 1.469 718.2

1 ppm 10.4 0.983 482.0

10 ppm 10.9 0.964 471.6

50 ppm 12.0 0.922 451.5100 ppm 12.7 0.898 439.6

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The effect of bleach solution with mangosteen extract

Concentration of bleach solution (ppm)

Absorbance value

0 20 40 60 80 100 1200

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

2

1:120 1:160 (1) 1:160 (2) 1:20021

Conclusion

Mangosteen peel extract solution can identify the concentration of bleaching reagent.

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Discussion The color of mangosteen

extract solutions were clearer when we put the bleaching solutions.

The higher concentration of bleaching solutions the lower absorbance.

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0 ppm

1 ppm

10 ppm50 ppm

100 ppm

Picture shown the mixture of bleach solution and mangosteen peel extract

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The plots of all ratios between mangosteen extract solution and bleaching solution have the same trend.

We use the absorbance value for drawing graph instead of the others because it is normally use.

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Benefit We can use mangosteen extract

solution to identify the concentration of bleach reagent by natural substance.

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Future research Testing other kinds of bleach

reagent such as sodium hydrosulphite instead of sodium metabisulphite.

Develop paper testing for bleaching reagent like Garnier paper.

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ReferencesWilson, D. T., Vaughan, D., Wilson, S. K., Simon, C. N. &

Lockhart, K. ( April 2008). Efficacy of a starch-iodine swab technique to detect the illegal use of bleach in a Spiny Lobster (Panulirus argus) fishery. Fisheries Research. Volume 90, Issues 1-3, Pages 86-91. Retrieved from http://www.sciencedirect.com.ejournal.mahidol.ac.th/science/article/pii/S0165783607002494.

Zanini, G.M., & Teixeira, C. G.(15 January 2001). Inactivation of infective larvae of Angiostrongylus costaricensis with short time incubations in 1.5% bleach solution, vinegar or saturated cooking salt solution. Acta Tropic. Volume 78, Issue 1, Pages 17-21.Retrieved from http://www.sciencedirect.com.ejournal.mahidol.ac.th/science/article/pii/S0001706X00001650

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Zock, J.P. et. al. (October 2009). Domestic use of hypochlorite bleach, atopic sensitization, and respiratory symptoms in adults. Journal of Allergy and Clinical Immunology. Volume 124, Issus 4, Page 731-738. Retrieved from http://www.sciencedirect.com.ejournal.mahidol.ac.th/science/article/pii/S0091674909008744

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Acknowledgements Dr. Usa Jeenjenkit and our

SINOS teachers Chemistry Department and

Biology Department of Mahidol Wittayanusorn School

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Thank youfor your attention

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