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  • THAI AGRICULTURAL STANDARD

    TAS 10300-2008

    DIAGNOSIS OF AVIAN INFLUENZA

    National Bureau of Agricultural Commodity and Food Standards Ministry of Agriculture and Cooperatives

    ICS 11.220 ISBN 978-974-403-565-3

  • UNOFFICAL TRANSLATION

    THAI AGRICULTURAL STANDARD

    TAS 10300-2008

    DIAGNOSIS OF AVIAN INFLUENZA

    National Bureau of Agricultural Commodity and Food Standards Ministry of Agriculture and Cooperatives

    50 Phaholyothin Road, Ladyao, Chatuchak, Bangkok 10900 Telephone (662) 561 2277 www.acfs.go.th

    Published in the Royal Gazette Vol.125 Special Section 139 D,

    dated 18 August B.E.2551 (2008)

  • (2)

    Ad hoc Sub-Committee on the Elaboration of Standard for Diagnosis of avian influenza

    1. Mr. Thanit Anekwit Chairperson Director General of the Department of Livestock Development

    2. Representative of the Department of Livestock Development

    Ms. Orapan Pasavorakul 3. Representative of the National Bureau of Agricultural Commodity

    and Food Standards Mrs. Orathai Sinraphanaphaphorn Ms. Yupa Laojindapun (alternate) 4. Representative of the Bureau of Disease Control and Veterinary Service Mr. Prasit Chaitaweesub Mrs. Chantanee Buranathai (alternate) 5. Representative of the Faculty of Veterinary Science,

    Chulalongkorn University Associate Professor Roongroje Thanawongnuwech Assistant Professor Thawat Lekdumrongsak (alternate) 6. Representative of the Faculty of Veterinary Science,

    Kasetsart University Associate Professor Thaweesak Songserm 7. Representative of the Faculty of Veterinary Science,

    Mahidol University Mrs. Pacharaporn Anankeatikul 8. Representative of the Faculty of Agriculture, Kasetsart University Associate Professor Supaporn Isariyodom 9. Representative of the Veterinary Council of Thailand Associate Professor Jiroj Sasipreeyajan Assistant Professor Somsak Pakpinyo (alternate) 10. Representative of the Thai Chamber of Commerce Ms. Pornsri Laorujisawat 11. Experts

    Mrs. Arunee Chaisingh Assistant Professor Theerapon Sirinarumitr Mrs. Autumporn Srisatidnarakul

  • (3)

    12. Representatives of the National Bureau of Agricultural Commodity and Food Standards

    Ms. Sakranmanee Krajangwong Secretary Ms. Mintra Lukkana Assistant Secretary

  • (4)

    Thai Agricultural Standard (TAS) on Diagnosis of Avian Influenza prescribes diagnostic methods for approval of avian influenza free and uses for an avian influenza diagnostic manual in laboratory for proper and precise procedures. It includes sampling methods and laboratory diagnostic techniques i.e. virology, immunology and bio-molecular. It promotes poultry production system and poultry products to yield good quality products and safety for consumption both domestically and internationally. It is also essential for notifying country status of avian influenza to Thailands trading partners as well as the World Organization for Animal Health (OIE). The establishment of this standard is based on the information of the following documents: The Study project on the status of important contagious poultry diseases in Thailand conducted by National Bureau of Agricultural Commodity and Food Standards B.E. 2548 (2004) in cooperation with Faculty of Veterinary Medicine, Chulalongkorn University. Word Organization for Animal Health (OIE). 2005. Chapter 2.7.12. Avian Influenza. Manual of Diagnostic Tests and Vaccines for Terrestrial Animal. Paris, France.

    Remark:

    The standard title has been revised from Thai Agricultural Commodity and Food Standard (TACFS) to Thai Agricultural Standard (TAS) in accordance with the enforcement of the Agricultural Standards Act B.E. 2551 (2008)

  • NOTIFICATION OF THE NATIONAL COMMITTEE ON AGRICULTURAL COMMODITY AND FOOD STANDARDS

    SUBJECT: THAI AGRICULTURAL COMMODITY AND FOOD STANDARD: DIAGNOSIS OF AVIAN INFLUENZA

    B.E. 2551 (2008)

    The resolution of the 1/2551 session of the National Committee on Agricultural

    Commodity and Food Standards dated 11 August B.E. 2551 (2008) endorsed the Thai Agricultural Commodity and Food Standard entitled Diagnosis of Avian Influenza. This standard would be of benefits for quality improvement, facilitating trade and protecting consumers.

    By virtue of the Cabinet Resolution on Appointment and Authorization of the National Committee on Agricultural Commodity and Food Standards dated 5 August B.E. 2551 (2008) the Notification on Thai Agricultural Commodity and Food Standard entitled Diagnosis of Avian Influenza is hereby issued as voluntary standard, the details of which are attached herewith.

    Notified on 14 August B.E. 2551 (2008)

    Mr. Somsak Prissana-nanthakul

    Minister of Agriculture and Cooperatives

    Chairperson of the National Committee on Agricultural Commodity and Food Standards

  • TAS 10300-2008

    THAI AGRICULTURAL STANDARD DIAGNOSIS OF AVIAN INFLUENZA

    1 SCOPE

    This Standard applies to defines criteria for diagnosis of avian influenza which is caused by influenza virus type A, subtype H5 and H7.

    2 DEFINITIONS

    For the purpose of this standard: 2.1 Avian Influenza means a severe contagious disease of poultry caused by influenza virus type A, subtype H5 and H7. 2.2 Avian means poultry including birds, chicken, ducks and geese. 2.3 Diagnosis means detection of sick or dead or suspected poultry to be infected with avian influenza virus by using laboratory methodologies together with history inquiries from related persons and clinical signs observation. 2.4 Laboratory Biosafety means a laboratory established with an appropriate system of construction, operation and examination as well as a safety operational procedures. Factors i.e. equipments, knowledge and technology shall be in place to prevent personnel, laboratory and environments from biohazards such as disease organisms, blood tissues, genetic materials and toxin which will enable to safe work with these hazardous substances. 3 DIAGNOSIS Avian Influenza diagnostic methods in laboratory include virology, immunology and bio-molecular which are indicated in the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (OIE).

    Avian Influenza is a severe contagious disease which is a notifiable disease in the Animal Epidemic Act (Annex A) and there is a high risk of virus spreading from the laboratory. In order to reduce such risk, the laboratory shall have a biosafety system covering building, equipments, and operational procedures, as well as training personnel for specific skills related to good laboratory practices, risk group of microorganisms, handling of samples or infected materials, and control of germs spreading. Laboratory biosafety system shall be regulary audited.

    3.1 SPECIMEN COLLECTION AND SENDING SAMPLES Samples collecting shall be appropriate using proper methods and sending to laboratory as soon as possible in order to have a precise and effective disease diagnosis in laboratory. The samples used for avian influenza diagnosis can be collected from sick animals or suspected animals to be infected with avian influenza or freshly dead carcasses before the post mortem autolysis occur. 3.1.1 Specimen collection from live poultry (1) Secretion from respiratory system by using sterile stick with polyester on its tip to swab mucous secretion from choanal slit, trachea and then dip the swab mucous secretion

  • TAS 10300-2008

    2

    from choanal slit into viral transport media such as phosphate buffer saline (PBS) or other kinds of viral transport media which are added with antibiotics as prescribed in Annex B.

    The collected cotton swab samples may be kept in the same viral transport media but not over than five sticks per tube.

    (2) Excretion from cloaca by using sterile cotton swab on the inner wall of cloaca or the collection of fresh faeces at least 1 g. The collected cotton swab samples may be kept in the same viral transport media but not over than five sticks per tube. 3.1.2 Samples taken from dead birds shall be whole bird carcass or collected organs such as trachea, lungs, spleen, liver, pancrease, intestine, kidney, heart and brain, etc. and put into suitable container separately. 3.1.3 Samples shall be sent to the laboratory immediately or within 24 hours by keeping the samples in a tightly closed container to prevent spreading the disease with the controled temperature at 4 C (2 C to 8 C). The samples shall not be frozen. 3.2 Diagnosis of Avian influenza Diagnosis of avian influenza subtypes H5 and H7 shall follow the standard methods which comprises two steps as the followings (details in Annex C): (1) Virus isolation by inoculating the sample into hatching chicken eggs and by haemagglutination test (HA test) for detecting a property of red blood cells precipitation. This step shows the virus is virus influenza type A or other pathogens that have same property. If the HA test is positive then proceed to next step in (2). (2) Virus identification: This step covers both testing for avian virus influenza type A and subtypes by AGID (Agar Gel Immunosorbent Assay) which is a precipitation in agar or by ELISA (Enzyme Linked Immunosorbent Assay) or by other methods recommended by the OIE for confirmation as inf