dietary canola oil reduces breast cancer risk laboratory of lactation north dakota state university
TRANSCRIPT
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Dietary Canola Oil Reduces Breast Cancer Risk
Laboratory of LactationNorth Dakota State University
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Outline Background
Previous Studies
Related Work
Recent Project
Ongoing Research
Future Study
Implications
Public Interest
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Background Since 2007 we have been
studying the role of canola oil on breast cancer risk (supported by the USDA and Northern Canola Growers Association)
The rationale was based on the fatty acid composition of canola oil
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Background Canola oil has an omega-6
to omega-3 ratio of 2:1
61% is oleic acid
Studies have linked this ratio and oleic acid with a reduced breast cancer risk
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Previous Studies
Canola Oil
Cell culture model
Animal model
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Cell Culture Model
Human breast cancer cells were treated with canola oil
Cell growth was measured
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Growth of Human Breast Cancer Cells
**
*
*
0
50
100
150
0 24 48 72 96
Cell
gro
wth
(%
)
Hours after canola oil treatment
Control
CAN
*P < 0.05
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Rat Model
Rats fed a diet containing canola oil or corn oil
Tumor induction by chemical carcinogen
Tumor development and measurement monitored
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Tumor Measurements
Corn Oil Canola Oil
Tumor Incidence 71% 57%
Latency Period 93 days 103 days
Number of Tumors 2 per rat 1.5 per rat
Tumor Volume 2.3 mm3 1.6 mm3
25%
30%
11%
20%
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Publication
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Related Work, Other Labs
Marshall University School of Medicine
South Dakota State University
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Recently Completed Project
Drug resistance is a major obstacle in breast cancer treatment
Effect of canola oil on drug resistance in breast cancer treatment
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Phase 1
Nude mice fed canola oil or soybean oil diet
Mice injected with drug-resistant cancer cells (DOX resistant)
Tumor development and growth monitored
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Phase 2 At 5 x 5 mm, mice were divided into 4 groups:
Soybean oil (SOY)
Canola oil (CAN)
SOY plus DOX treatment
CAN plus DOX treatment
Tumor growth rate was recorded.
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0
0.4
0.8
1.2
1.6
2
0 1 2 3 4 5 6 7Lo
g s
ca
led
tu
mo
r v
olu
mes (
mm
3 )
Weeks after initial tumor detection
SOY
CAN
Tumor Sizes Before Drug Treatment
*
*
*P < 0.05
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0
0.4
0.8
1.2
1.6
2
SOY SOY plus DOX CAN CAN plus DOX
Rela
tive t
um
ore
volu
me
Treatments
SOY
SOY plus DOX
CAN
CAN plus DOX
Tumor Sizes After Drug Treatment
32%
48% 23%
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This work has been submitted to the journal Lipids for publication
Publication
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Lipotropes Research
Our laboratory has been investigating the role of dietary methyl nutrients (lipotropes) on breast cancer risk
Lipotropes include vitamin B12, choline, folic acid, and methionine
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Lipotropes ResearchRecently showed that maternal
lipotropes reduce breast cancer risk in offspring
Supported by department of defense (DOD)
Findings were presented at the Era of Hope conference (one of the premiere breast cancer research meetings)
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Based on the lipotropes and canola oil studies, we then began studying the effect of dietary lipotropes plus canola oil on breast cancer risk
This concept is called Lipocan
Ongoing Research
Lipocan
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This project is sponsored by the NCGA
The purpose of the study is to gather data to apply for a US patent
This patent is aimed at benefiting NDSU research and NCGA
Ongoing Research
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Ongoing Research
We are using cell culture and animal model
Cell culture component is complete
The animal trial will commence on the 21st of Nov 2011
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Lipocan Results
Cell Growth Inhibition at 72 hr (%)
Lipocan vs Control 35
Lipocan vs Canola 14
Lipocan vs Lipotropes 25
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The first part of the animal trial is to test the lipocan concept on a simple animal model
Mice will be fed the lipocan diet and then injected with a carcinogen to induce mammary cancer as previously mentioned
Ongoing Research
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To complete this project, we must include the effect of a maternal lipocan diet on the risk of breast cancer of the babies
Thus, the lipocan project will require 2 years to complete for the US patent application
Ongoing Research
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We included the pregnancy experiment based on:
Researchers are focusing on reducing cancer risk of the babies through proper maternal diet and lifestyle
Also, findings on maternal lipotropes and canola oil suggest they both reduce breast cancer risk of the offspring
Ongoing Research
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Ultimately, the lipocan data from the 2-year project will serve as the basis for a full-scale NIH grant application
Future Study
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Patent:
How does the patent work and how do you stand to benefit from it?
Development of a Lipocan nutraceutical supplement to benefit human health
Direct economic benefits for canola growers
Implications
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NDSU recently organized a Moos, Ewes and More event
We presented our canola oil and breast cancer research
The interest from the public was very encouraging
Public Interest
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Team
From Left: Dr. Chung Park, Lawrence Mabasa, Sajin Bae, Mark Walters, and Kyongshin Cho
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AcknowledgmentsNorthern Canola Growers Association
USDA-NIFA