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ODUCTION: Thweeks after yo

aneous remyelined by demyelinas have demonstonal Kurtosis Im

onal information branes, organellestructural compleis metrics provid

opment15 and in dand white matter HODS: A total ofd mouse chow elination. The conystem. A respiratlice thickness=1

ent direction (0.5axial (K//) and ra

k, MA) called Diffs of interest (RO

al (vHP)) were murements betweeLTS & DISCUSS

ogical stain (Solom demyelination.previous reports, ghout the entire Cnd K┴ throughoutstent with the d

ment). The axial dared to that of ed only in the bCstages of patholoaxial diffusion ch

ogical correlation┴ showed signifis cortical demyeexity not only in with demyelinatimalities in grey m

Diffusionah A Helpern, 1, DavUniversity of Sout

United St

he cuprizone mooung adult mice ation occurs2,3.

ation associated trated the patho

maging (DKI) is a beyond that pro

es) and water coexity in the greydes better differedifferent diseaseduring the demyf 22 (8–10 weekscontaining cuprntrol (NC) group tion-gated 4-shot mm, data matr, 1, 1.5, 2 and 2adial (K┴) kurtosifusional KurtosisOIs) at the level manually drawnen CPZ and NC mSION: Illustratedochrome) for NC. Fig.2 shows theCPZ mice show

CC. Also, the nont the entire CC indegree of demydiffusivity λ// wasNC, and the K//

CC. Since axonaogy (before 4 wehanges (at 10 wen. A striking resuicant changes (elination and reawhite matter, buon induced by c

matter.

Fig.2. cuprizoregionand c(dorsaare sta

al Kurtosis Detvid Guilfoyle2, Scoth Carolina (MUSCtates, 3Dementia R

ouse model is a are fed with th

In this model, dwith a microglialogy of the CC idiffusion MRI tec

ovided by DTI12-1

ompartments (exy matter in additntiation of brain

es processes6,18. yelination processs old) C57BL/6 mizone (0.2%), (B(n=10) was maint SE-EPI sequenrix=128×128, im.5 ms/μm2). Fracs were derived f

s Estimator (DKEof corpus callos using ImageJ mice. P < 0.05 w in Fig.1 are rep

C and CPZ moue ROI measuremwed significantlyn-Gaussian diffusn the CPZ mice. yelination seen s increased in th/ showed a trenl damage in this eeks of cuprizoneeks of cuprizonult was that onlydecrease) in the

active glial cells ut also in grey macuprizone, demo

ROI measuremeone treated (CPZ)s: corpus callosum

caudal (pCC)), col (dHP) and ventra

andard deviations.

tects Cortical ott Gerum2, CaixiaC), Charleston, SC

Research, Nathan K

well characterizhe copper chelademylination is pal response3,4. Rein cuprizone mochnique that exte4. Since non-Ga

xtracellular and intion to white matissue type, and The main goal o

s in the cuprizonmice were used Bis(cyclohexanonntained on a nornce was used fo

mage resolution=ctional anisotropfrom the DKI dat

E))20. All parametum (rostral (aCC((http://rsb.info.n

was considered apresentative b0 im

se brain. Solochment of different y reduced FA ansion metrics showThese diffusion at this phase (

he bCC, but decnd for decrease

model is variablne treatment)7, the treatment) can

y the non-Gausse cortex of the accumulation5, catter. In summaronstrating the sig

ents of diffusion ) and control (NC)m (rostral (aCC), mortex (CT) and al (vHP)); * p < 0.0

Demyelinatioa Hu2, John LaFranC, United States, 2MKline Institute, Ora

zed animal modetor cuprizone (b

predominantly foecently, cortical

ouse model6-11. Hends DTI and quaussian diffusionntracellular), the

atter structures. are sensitive to

of this study wase mouse model.in this study. In ne) oxaldihydrazmal diet for 10 wr DKI acquisition234×234 μm2, 4

py (FA), mean (Mta set14 using antric maps were mC), middle (bCC)nih.gov/ij/). Two-s statistically sig

mages, MK mapshrome was perfobrain regions. Cnd increased MDwed significantlychanges are qua(10 weeks of creased in the pC, but it was sigle and more promhe exact interpre

n only be done wian diffusion meCPZ mice, wh

confirming that ry we observed, gnificant advant

metrics for ) mice. Brain

middle (bCC), hippocampus

05. Error bars

on in the Cuprncois3, Xingju NieMedical Physics, Nangeburg, New Yo

el of demyelinatbis-cyclohexanonound in the corp

demyelination hHowever, no coruantifies the non-n is believed to ae measures of D

Indeed, severachanges in brain

s to quantitatively the cuprizone (Czone, Sigma-Ald

weeks. All in vivo n. The sequence4 averages, 30

MD), axial (λ//) ann in-house softwamasked (MD> 1.5), and caudal (pC-tailed t-test wa

gnificant. s and the ormed to onsistent D and λ┴ y reduced alitatively cuprizone CC when gnificantly minent in etation of

with future etrics MK, ich likely kurtosis metricsfor the first time

tage of microstru

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supported by NI34-01.

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3066Proc. Intl. Soc. Mag. Reson. Med. 20 (2012)