dimensional stability of impression materials disinfected ... · siloxane impression material. •...

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1 PhD Program in Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA 2 Division of Operative Dentistry, Department of General Dentistry, University of Maryland School of Dentistry, Baltimore, MD 21201, USA 3 Division of Pediatric Dentistry, Department of Orthodontics and Pediatric Dentistry, University of Maryland School of Dentistry, Baltimore, MD 21201, USA 4 Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA Dimensional Stability of Impression Materials Disinfected by COEfect TM MinuteSpray Kuei-Ling C. Hsu 1,3 , Louis DePaola 4, Mary Anne S. Melo 1,2 Disinfection of clinical infected materials is a part of important and necessary steps for proper infection control protocol in dentistry. Spray surface disinfectant is commonly used to disinfect impression of fixed and removable prosthesis. A few commercial surface disinfectants are available to use in dental impression. However, the effect of these surface disinfectant in dimensional and morphological changes are yet to be determined. Example of the content in these disinfectants is listed below. The purpose of this study was to determine the effect of commercially available disinfectant solutions on the dimensional stability and surface quality of two types of impression materials available to clinicians: dental alginate and polyvinyl siloxane impression materials. INTRODUCTION MATERIALS AND METHODS RESULTS CONCLUSION Two types of impression materials were evaluated: alginate and polyvinyl siloxane (PVS). Four one-step spray antimicrobial disinfectants (BirexSE®, Opti-Cide3®, COEfect™ MinuteSpray, CaviCide ) along with control (distilled water) were used to disinfect the edentulous impression by both materials according to the manufacture instruction. Procedures were repeated in triplicates. To evaluate the dimensional stability, the impression was scanned before and after disinfectant spray by a CBCT scanner (Carestream 9300, Atlanta, GA) and revered to a 3D positive image using related Kodak software. Linear measurement were obtained on the digital model using 3 fixed reference points. ANOVA one-way and Tukey test were used to analyze the difference of linear changes for each disinfectant. Significant level was set at p<0.05. To evaluate the surface quality of the impression, a representative specimen of each group were prepared and examined with electronic scanning microscopy (SEM). All specimens were examined under an FEI Quanta 200 operating at 25kV and X500 magnification. Fig. 1: Localization and demarcation of reference metallic points on maxillary complete edentulous template. Disinfection of the impressions by the tested commercially available disinfectant solutions did not yield significant linear dimensional changes in dimensions and morphological alteration of the used dental alginate and polyvinyl siloxane impression material. The disinfection of impression materials frequently used in the dental clinical procedures such alginate and polyvinyl siloxane impression material can be performed without detrimental to the dimensional stability of the material. Fig. 5: Mean and standard deviation of dimensional differences of dental alginate subjected to disinfection with different disinfecting solutions. The horizontal line indicates that all five groups had a similar dimensional difference (p > 0.5). Fig. 6: Mean and standard deviation of dimensional differences of polyvinyl siloxane impression material subjected to disinfection with different disinfecting solutions. The horizontal line indicates that all five groups had a similar dimensional difference (p > 0.5). Fig. 7: SEM micrograph of dental alginate and polyvinyl siloxane impression material subjected to water (control group) and COEfect™ MinuteSpray , respectively, presenting no morphological alterations (×500). ACKNOWLEDGEMENTS This project is supported by GC America Inc. USA. Fig. 2: Trays loaded with dental alginate impression before the disinfection procedures. Fig. 3: Impressions were scanned immediately after impression-taking with a CBCT scanner (Carestream 9300, Atlanta, GA) Fig. 4: Fabrication of a digital dental model from the CBCT scan of impressions: A: the impression was scanned by CBCT; the negative form of the image was changed into positive form via software 3D image reconstruction. B: linear measurements were made on the digital models in the A-B, A-C and B-C dimensions. Dimensional Stability Surface Quality #3721

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Page 1: Dimensional Stability of Impression Materials Disinfected ... · siloxane impression material. • The disinfection of impression materials frequently used in the dental clinical

1 PhD Program in Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA 2 Division of Operative Dentistry, Department of General Dentistry, University of Maryland School of Dentistry, Baltimore, MD 21201, USA 3 Division of Pediatric Dentistry, Department of Orthodontics and Pediatric Dentistry, University of Maryland School of Dentistry, Baltimore, MD 21201, USA 4 Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA

Dimensional Stability of Impression Materials Disinfected by COEfectTM MinuteSpray

Kuei-Ling C. Hsu1,3, Louis DePaola4, Mary Anne S. Melo1,2

• Disinfection of clinical infected materials is a part of important and necessary

steps for proper infection control protocol in dentistry.

• Spray surface disinfectant is commonly used to disinfect impression of fixed

and removable prosthesis. A few commercial surface disinfectants are

available to use in dental impression. However, the effect of these surface

disinfectant in dimensional and morphological changes are yet to be

determined. Example of the content in these disinfectants is listed below.

• The purpose of this study was to determine the effect of commercially available

disinfectant solutions on the dimensional stability and surface quality of two

types of impression materials available to clinicians: dental alginate and

polyvinyl siloxane impression materials.

INTRODUCTION

MATERIALS AND METHODS

RESULTS

CONCLUSION

• Two types of impression materials were evaluated: alginate and polyvinyl

siloxane (PVS).

• Four one-step spray antimicrobial disinfectants (BirexSE®, Opti-Cide3®, COEfect™ MinuteSpray, CaviCide ) along with control (distilled water) were used

to disinfect the edentulous impression by both materials according to the

manufacture instruction. Procedures were repeated in triplicates.

• To evaluate the dimensional stability, the impression was scanned before and

after disinfectant spray by a CBCT scanner (Carestream 9300, Atlanta, GA)

and revered to a 3D positive image using related Kodak software. Linear

measurement were obtained on the digital model using 3 fixed reference

points. ANOVA one-way and Tukey test were used to analyze the difference

of linear changes for each disinfectant. Significant level was set at p<0.05.

• To evaluate the surface quality of the impression, a representative specimen

of each group were prepared and examined with electronic scanning

microscopy (SEM). All specimens were examined under an FEI Quanta 200

operating at 25kV and X500 magnification.

Fig. 1: Localization and demarcation ofreference metallic points on maxillarycomplete edentulous template.

• Disinfection of the impressions by the tested commercially available disinfectant solutions did not yield significantlinear dimensional changes in dimensions and morphological alteration of the used dental alginate and polyvinylsiloxane impression material.

• The disinfection of impression materials frequently used in the dental clinical procedures such alginate and polyvinylsiloxane impression material can be performed without detrimental to the dimensional stability of the material.

Fig. 5: Mean and standarddeviation of dimensional differencesof dental alginate subjected todisinfection with differentdisinfecting solutions. The horizontalline indicates that all five groups hada similar dimensional difference (p >0.5).

Fig. 6: Mean and standarddeviation of dimensional differencesof polyvinyl siloxane impressionmaterial subjected to disinfectionwith different disinfecting solutions.The horizontal line indicates that allfive groups had a similardimensional difference (p > 0.5).

Fig. 7: SEM micrograph ofdental alginate and polyvinylsiloxane impression materialsubjected to water (control group)and COEfect™ MinuteSpray ,respectively, presenting nomorphological alterations(×500).

ACKNOWLEDGEMENTS

• This project is supported by GC America Inc. USA.

Fig. 2: Trays loaded with dental alginate impression before the disinfectionprocedures.

Fig. 3: Impressions were scannedimmediately after impression-takingwith a CBCT scanner (Carestream 9300,Atlanta, GA)

Fig. 4: Fabrication of a digital dental model from the CBCT scan ofimpressions: A: the impression was scanned by CBCT; the negative form ofthe image was changed into positive form via software 3D imagereconstruction. B: linear measurements were made on the digital models inthe A-B, A-C and B-C dimensions.

Dimensional Stability

Surface Quality

#3721