Disease update 2018

Sabine Banniza

CROP DEVELOPMENT CENTRE

The year that was …..

Early and late season precipitation in SK Based on SK MoA Crop Reports

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Aril - June July - October

* Estimated from Saskatchewan Research Council Graph

2017 Seed Quality Chickpea Collated from 20/20, Discovery, and Prairie Diagnostic by B. Olsen

Ascochyta Botrytis Sclerotinia Crop District # Tests % PFS Mean % % PFS Mean % % PFS Mean % 1A 1 100 0 100 0 100 0 1B 0 nd nd nd nd nd nd 2A 18 100 0 100 0 100 0 2B 15 86.7 1.8 100 0 100 0 3AN 7 100 0 100 0 100 0 3AS 53 98.1 0.3 100 0 100 0 3BN 1 100 0 100 0 100 0 3BS 4 100 0 100 0 100 0 4A 0 nd nd nd nd nd nd 4B 0 nd nd nd nd nd nd 5A 0 nd nd nd nd nd nd 5B 0 nd nd nd nd nd nd 6A 0 nd nd nd nd nd nd 6B 4 100 0 100 0 100 0 7A 0 nd nd nd nd nd nd 7B 0 nd nd nd nd nd nd 8A 0 nd nd nd nd nd nd 8B 0 nd nd nd nd nd nd 9A 0 nd nd nd nd nd nd 9B 0 nd nd nd nd nd nd Total/Mean 103 94.3 1.3 100 0 100 0

2017 Seed Quality Lentil Collated from 20/20, Discovery, and Prairie Diagnostic by B. Olsen

Ascochyta Anthracnose Botrytis Sclerotinia Crop District # Tests % PFS Mean % % PFS Mean % % PFS Mean % % PFS Mean %

1A 7 100 0 100 0 85.7 0.5 100 0 1B 2 100 0 100 0 100 0 100 0 2A 44 100 0 100 0 100 0 100 0 2B 92 98.9 1 100 0 96.7 1.4 98.9 2

3AN 18 100 0 100 0 100 0 94.4 0.3 3AS 93 100 0 98.9 0.3 93.5 2.2 94.6 1.3 3BN 63 100 0 98.4 0.8 93.7 0.8 95.2 0.5 3BS 16 100 0 100 0 100 0 100 0 4A 4 100 0 100 0 100 0 100 0 4B 7 100 0 100 0 100 0 100 0 5A 12 100 0 100 0 100 0 100 0 5B 0 nd nd nd nd nd nd nd nd 6A 37 100 0 91.9 0.6 81.1 0.8 91.9 0.5 6B 87 97.7 0.3 97.7 0.9 88.5 0.5 95.4 0.5 7A 79 98.7 0.3 89.9 0.6 83.5 0.8 91.1 0.5 7B 12 100 0 100 0 100 0 88.9 0.3 8A 1 100 0 100 0 100 0 100 0 8B 2 100 0 100 0 100 0 100 0 9A 1 100 0 100 0 100 0 100 0 9B 2 100 0 100 0 100 0 100 0

Total/Mean 579 99.3 0.5 95.7 0.7 92 0.9 95.7 0.7

2017 Seed Quality Pea Collated from 20/20, Discovery, and Prairie Diagnostic by B. Olsen

Ascochyta Botrytis Sclerotinia Crop District # Tests % PFS Mean % % PFS Mean % % PFS Mean %

1A 14 100 0 100 0 100 0 1B 10 80 0.5 100 0 100 0 2A 9 100 0 100 0 87.5 1.5 2B 39 92.3 0.5 100 0 100 0

3AN 5 100 0 100 0 100 0 3AS 27 88.9 0.5 100 0 100 0 3BN 17 64.7 1 94.1 0.5 100 0 3BS 2 100 0 100 0 100 0 4A 3 33.3 4.8 66.7 1.5 100 0 4B 7 85.7 1.5 100 0 100 0 5A 12 91.7 2 100 0 100 0 5B 12 75 0.7 72.7 0.5 100 0 6A 35 85.7 0.5 100 0 100 0 6B 91 70.3 0.8 100 0 100 0 7A 29 62.1 1.7 96.6 0.5 96.6 0.5 7B 30 53.3 0.8 85.7 0.5 96.4 0.5 8A 10 70 0.6 90 0.5 100 0 8B 31 80.6 1.5 96.8 0.5 100 0 9A 21 57.1 1.4 85 0.6 95 0.5 9B 49 20.4 2.9 20.6 0.6 100 0

Total/Mean 453 70.2 1.6 90 0.6 99.1 0.8

Root rot incidence and severity S. Chatterton, M. Harding, R. Bowness, C. Vucurevich, T. Dubitz, J. Nielsen, B. Gossen,

S. Banniza, B. Ziesman, D. McLaren

No. fields surveyed

Root rot prevalence

(%)

Root rot incidence

Root rot incidence

> 3 (%)

Average severity

Pea east-central AB 35 100 91 36 3.3 west-central AB 89 100 73 31 2.8 southern AB 35 85 54 19 2.2 Alberta (Total) 159 97 73 30 2.8 Saskatchewan 100 100 93 43 3.5 Manitoba 31 100 97 36 3.3

Lentils southern AB 15 100 80 3.7 2.1 Saskatchewan 83 100 78 9.5 2.2

Dry bean southern AB 15 40 40 0 1.5

Aphanomyces Root Rot: Infectivity model for Aphanomyces euteiches in Saskatchewan soils

Syama Chatterton, AAFC Lethbridge

With how many Aphanomyces spore can a pea plant happily live?

Molecular quantification

Disease severity Correlation

Aphanomyces Root Rot: Infectivity model for Aphanomyces euteiches in

Saskatchewan soils Syama Chatterton, AAFC Lethbridge

Research team: Syama Chatterton (AAFC Lethbridge)

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Field Low Medium High 0-20cm GH-CtrlST 3.3 1MC 2.7 1ML 3.4 1EL 4.4 1SV 4.8 1GL 3.0 1LA 1.9 1MJ 3.3 1Ed` 1.6 1MS 3.2 1Ws 1.9 1

Disease Severity Disease Rating

2017 Field testing

Southy (Dark Brown) Maple Creek (Brown) Meadow Lake (Grey) Eldersley (Grey) Slyvania (Grey) Gull Lake (Brown) Lafleche (Brown) Moose Jaw (Dark Brown) Raymore (Black) Maidstone (Black) Watrous (Dark Brown)

DEVELOPING PEAS WITH BETTER APHANOMYCES

Lavaud et al 2015. Theor Appl Genet 128(11),2273-88

French pea map with Aphanomyces resistance QTLs

Lavaud et al 2015. Theor Appl Genet 128(11),2273-88

Developing peas with better Aphanomyces

• Crossing with – USDA material with

partial resistance (QTL Ae-Ps7.6)

– 90-2079 with partial resistance (QTL Ae-Ps4.5)

Parent 1 Parent 2 CDC 2847-21 /PI 660733 CDC 2847-21 CDC 2936-7 CDC 2950-19/PI 660736 CDC 2950-19/PI 660736 CDC 2936-7 CDC 3007-6 CDC 3007-6 /PI 660733 CDC 3422-8/PI 660729 CDC 3422-8 CDC 3422-8/PI 660736 CDC 3422-8 CDC 4053-4/PI 660736 CDC 4053-4 CDC Amarillo/PI 660729 CDC Amarillo

Parent 1 Parent 2 CDC 2847-21/90-2079 CDC 2847-21 CDC 2936-7/90-2079 CDC 2936-7 CDC 2950-19/90-2079 CDC 2950-19 CDC 3007-6/90-2079 CDC 3007-6 CDC 3422-8/90-2079 CDC 3422-8 CDC 4053-4/90-2079 CDC 4053-4

CDC Amarillo (yellow); CDC 2847-21 (CDC Inca – yellow); CDC 2950-19 (yellow); CDC3007-6 (CDC Spruce – green); CDC 3422-8 (green); CDC 3422-8 (green); CDC 4053-4 (green)

QTL Ae-Ps7.6 QTLs Ae-

Ps7.6 + Ae-Ps4.5

QTL Ae-Ps4.5

BC1F2 seeding

Apr 2017

Mar 2017

Parent1 x Parent2

Back crossing

Jul 2017

May 2017

F1 & back-

crossing

BC2F1 seeds

Aug 2017

Aug 2017

BC1F1 seeds,

BC2 crossing

BC2F2 seeds

Sep 2017

Jan 2018

BC2F2 seeds

MAS Dec 2017

Feb 2018 MAS

BC2F3 May 2018

May 2018 BC2F3

Pheno-typing

Jul 2018

Jul 2018

Pheno-typing

MAS Aug 2018

Aug 2018 MAS

Pyramiding Nov 2018 Pyramid

ing MAS Dec 2018 MAS Seed

increase Spring 2019 Seed increase

Seed increase

Summer 2019

Seed increase

Yield testing

Summer 2020

Yield testing

IDENTIFYING LENTIL WITH BETTER APHANOMYCES RESISTANCE

Lavaud et al 2015. Theor Appl Genet 128(11),2273-88

Gene pools in lentil

• Closely related species (primary gene pool) can be easily crossed

• Crossing with distantly related species difficult

Aphanomyces resistance in wild lentil germplasm

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Aphanomyces resistance in wild lentil germplasm

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Pathotypes of Aphanomyces

• French have identified 11 “virulence” types (pathotypes) • Screened for resistance with pathotype I (most common in

France) and pathotype III (Ae109; most common in N-America?)

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BaccaraMN313CDC MeadowPI660736

Canadian USA

A successful 2018!

Agri-Food Canada Agriculture and

CROP DEVELOPMENT CENTRE