7/27/2019 Diversity of Ampeloviruses in Mealybug and Soft Scale Vectors and in Grapevine Hosts From Leafroll-Affected Vine

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October 2009, Volume 99, Number 10Pages 1177-1184http://dx.doi.org/10.1094/PHYTO-99-10-1177

Virology

Diversity of Ampeloviruses in Mealybug and Soft Scale Vectors andin Grapevine Hosts from Leafroll-Affected Vineyards

M. Fuchs, P. Marsella-Herrick, G. M. Loeb, T. E. Martinson, andH. C. Hoch

First, second, and fifth authors: Department of Plant Pathology and Plant-Microbe Biology; thirdauthor: Department of Entomology; and fourth author: Department of Horticultural Sciences, CornellUniversity, New York State Agricultural Experiment Station, Geneva, NY 14456.PDF Print (243 KB) |PDF with Links (245 KB)

Open Access.

The occurrence and diversity ofGrapevine leafroll-associated virus 1(GLRaV-1) and Grapevine leafroll-associated virus 3 (GLRaV-3) in the soft scales Parthenolecanium corniand Pulvinaria innumerabilis and in themealybug Pseudococcus maritimus was determined in leafroll-affected vineyards in the Finger Lakes region ofNew York. Groups of 1 to 4 specimens were collected under loose grapevine bark and tested by reverse-transcription polymerase chain reaction (RT-PCR) for segments of the second diverged copy of the GLRaV-1coat protein gene or GLRaV-3 heat-shock protein 70-homologue gene. Virus-specific RT-PCR products wereamplified from immature insect vectors and adult mealybugs. Single viral amplicons were obtained mostly fromimmature vectors (35%, 30 of 85) and dual viral amplicons from immature (16%, 10 of 61) and adult (100%,14 of 14) mealybugs, including individuals. These observations suggested a simultaneous uptake of GLRaV-1and GLRaV-3 by individual mealybugs. Furthermore, a comparative nucleotide sequence analysis of viralamplicons from soft scales, mealybugs, and grapevines from which vectors were collected showed identical orhighly similar haplotypes, indicating that uptake of GLRaV-1 and GLRaV-3 likely occurred by direct feeding ofvectors on their host plants.

Additional keywords: Closteroviridae, transmission, Vitis vinifera.

Cited by

Association of a DNA Virus with Grapevines Affected by Red Blotch Disease in CaliforniaMaher Al Rwahnih, Ashita Dave, Michael M. Anderson, Adib Rowhani,Jerry K. Uyemoto, and MysoreR. SudarshanaPhytopathologyOct 2013, Volume 103, Number 10: 1069-1076Abstract|PDF Print (700 KB)|PDF with Links (526 KB)Rapid detection of Grapevine leafroll-associated virus type 3 using a reverse transcriptionloop-mediated amplification methodHelen Ann Walsh and Gerhard Pietersen

Journal of Virological Methods Sep 2013CrossRefStylet penetration behaviour of Pseudococcus longispinus in relation to acquisition of

grapevine leafroll virus 3W. R. M. Sandanayaka, A. G. Blouin, E. Prado, and D. Cohen

Arthropod-Plant Interactions Apr 2013, Volume 7, Number 2: 137-146CrossRefOccurrence of grapevine leafroll-associated virus 5 in Portugal: genetic variability and

population structure in field-grown grapevinesFilipa Esteves, Margarida Teixeira Santos, Jos Eduardo Eiras-Dias, and Filomena Fonseca

Archives of VirologySep 2012, Volume 157, Number 9: 1747-1765CrossRefMacroarray detection of grapevine leafroll-associated virusesJeremy R. Thompson, Marc Fuchs, Kael F. Fischer, and Keith L. Perry

Journal of Virological Methods Aug 2012, Volume 183, Number 2: 161-169CrossRefGrapevine leafroll-associated virus 1 Occurs as Genetically Diverse Populations

Olufemi J. Alabi, Maher Al Rwahnih, Gandhi Karthikeyan, SudarsanaPoojari, Marc Fuchs, Adib Rowhani,and Rayapati A. Naidu

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7/27/2019 Diversity of Ampeloviruses in Mealybug and Soft Scale Vectors and in Grapevine Hosts From Leafroll-Affected Vine

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PhytopathologyDec 2011, Volume 101, Number 12: 1446-1456

Abstract|PDF Print (470 KB)|PDF with Links (373 KB)|Distribution of grapevine leafroll associated virus-3 variants in South African vineyardsAnna E. C. Jooste, Gerhard Pietersen, and Johan T. BurgerEuropean Journal of Plant PathologyMay 2011CrossRef

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