dna fingerprinting 1.how do we do it ? 2. what do we use it for?
TRANSCRIPT
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DNA Fingerprinting
1. How do we do it ?
2. What do we use it for?
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DNA Fingerprinting – How it’s done...
1. DNA has two complementary strands
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2. Extract DNA from cells – e.g. A blood sample containing white blood
cells
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3. Isolate DNA from cells & cut up DNA using enzymes
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5. Gene probe made = single strand complementary to part of DNA
A fluorescent marker is added to it
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6. Gene probe mixed with DNA sample
Probe binds to complementary base sequence that is being looked for
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7. DNA & probe is separated using gel electrophoresis
Smaller fragments travel further through the gel
50 second animation – gel electrophoresishttp://www.youtube.com/watch?v=PSwlCk_Z02c
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8. UV Light shone on sample – locates fragments with fluorescent marker
Smaller fragments travel further through the gel
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Uses of DNA Fingerprinting
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Uses of DNA Fingerprinting
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DNA Fingerprinting
Naked Science video 5 minshttp://www.youtube.com/watch?v=ZxWXCT9wVoI
Slightly more involved video 6 minshttp://www.youtube.com/watch?NR=1&feature=fvwp&v=DbR9xMXuK7c
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Nanotechnology
1. How small is a nanometre?
2. Size-wise....What could you compare it to?
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1. Use particles around 100 nanometres long/wide. About the same size as some molecules.
2. Human hair is 100 thousand nanometres in diameter!
Nanotechnology
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1. Silver particles embedded in plastic reduce microbial growth
2. Food wrapping films that change colour e.g. when;
Antibodies react with bacteria in food
Nanoparticles react with oxygen – sign of damage
Ripening fruit release gases
Nanotechnology
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1. Used to treat leukaemia – cancer of blood or bone marrow
2. Have potential to treat spinal cord injuries
3. In future, may be used to treat Parkinson’s, Alzheimer’s, Diabetes.
Stem Cells (B1 & B5)
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1. Replace heart valves
Biomedical Engineering
BUPA animation – 3 minshttp://www.youtube.com/watch?v=rkW1smPoXKA
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1. Artificial pacemakers in heart
Biomedical Engineering
http://www.youtube.com/watch?v=TjW5-pU0nBs