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DNA Isolation Group 10:

Kim Valenzuela

Janina Villaseran

Angelli Vivas

Kana Watabe

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PrinciplesDNA isolation is a process used to extract DNA from various sources.

Ethanol is used to precipitate out the DNA and then collected through DNA spooling. Click icon to add picture

+ ProceduresDissolve EDTA and

NaCl in 900 ml Distilled H2O.

Add 100 ml Axion liquid detergent.

Blend onion chunks with 100

ml of mixture.

Heat for 30 mins at 50°C.

Cool in an ice bath for 10 mins and

filter.

Collect 5 ml of filtered fluid and place in test tube.

Spool the DNA from the bottom of the test tube using

a stirring rod.

Add 10 ml of 95% chilled ethanol.

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Results

After completing the process of DNA isolation, a white substance should appear at the bottom of the test tube.

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Interpretations

Click icon to add picture

Click icon to add picture

The white stringy substance extracted after DNA spooling is the DNA from the onion.