dna microarray. biology statistics (data analysis) engineering (microfluidic, microfabrication,...
TRANSCRIPT
![Page 1: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/1.jpg)
DNA microarray
![Page 2: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/2.jpg)
BiologyStatistics(data analysis)
Engineering(microfluidic, microfabrication, robotic automation…)
Chemistry(surface modification)
Optics(signal detection)Electronics, physics
Research Integration
![Page 3: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/3.jpg)
Gene Expression
Gene Discovery
Genotyping (SNPs)Drug Discovery
Diagnosis
Sequencing/resequencing
Disease Barcoding, Drug Susceptibility
Crop ImprovementBasic Research
![Page 4: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/4.jpg)
Preparation of DNA solution for microarray
PCR amplification
PCR product purification
96-well plate
Quality control
Collections of ESTs, library clones...
DNA printing solution
384-well plate
Re-racking
![Page 5: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/5.jpg)
Modified from http://darwin.bio.uci.edu/~faculty/wagner/array2.html
Printing of DNA microarray
![Page 6: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/6.jpg)
Modified from http://darwin.bio.uci.edu/~faculty/wagner/array2.html
Each spot represents different gene/clone
![Page 7: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/7.jpg)
Flowers
Leaves
AAAAAAAAAA AAAAA
AAAAA AAAAA
AAAAAAAAAA
AAAAA
AAAAAAAAAA
mRNA isolation
DNA microarray probe preparation
cDNA labeling with fluorescent dyes
![Page 8: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/8.jpg)
Modified from http://darwin.bio.uci.edu/~faculty/wagner/array2.html
Flower
Leaf
DNA microarray hybridization
![Page 9: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/9.jpg)
Modified from http://darwin.bio.uci.edu/~faculty/wagner/array2.html
Labeled DNA hybridizes to corresponding DNA/gene
![Page 10: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/10.jpg)
Laser excitation
Cy5: ~650 nmCy3: ~550 nm
Image overlay
No changes
Flower up-regulated genes
Leaf up-regulated genes
![Page 11: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/11.jpg)
Cy3 labeled cDNA from etiolated seedlingsCy5 labeled cDNA from green leaves
Arabdidopsis 11.5K microarray
![Page 12: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/12.jpg)
Girke et al. 2000
Detection of cross hybridization
![Page 13: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/13.jpg)
Gygi et al. 1999
Correlation between protein and mRNA levels
![Page 14: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/14.jpg)
Plant functional genomics
Basic mechanism of plant growth and developmentBasic mechanism of plant growth and development
Application in agricultural biotechnologyApplication in agricultural biotechnology
增加農作物產量創造高營養價值之農作物創造具高適應力之農作物利用農作物作為生物反應器
![Page 15: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/15.jpg)
DNA microarrayand beyond….
![Page 16: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/16.jpg)
Insertion site identification from knockout population
Mahalingam and Fedoroff, PNAS (2001) 13, 7420-7425
![Page 17: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/17.jpg)
Pollack et al. 1999
DNA copy number detection
![Page 18: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/18.jpg)
Iyer et al. Nature (2001) 409,533-38
DNA-protein interaction
![Page 19: DNA microarray. Biology Statistics (data analysis) Engineering (microfluidic, microfabrication, robotic automation…) Chemistry (surface modification)](https://reader035.vdocument.in/reader035/viewer/2022062722/56649f2b5503460f94c45801/html5/thumbnails/19.jpg)
MacBeath et al. (2000) Science 289, 1760-3
Protein-protein interaction (protein microarray)