dna repair pathways poster
TRANSCRIPT
Polε
Msh2 Msh6 (MutSα)
Msh2 Msh6 (MutSα)
MutLα
endonuclease
activity and
Exo1 remove
mismatch by
5’ to 3’ resection
RFC/PCNA and
endonuclease
activity of MutLαallow Exo1 to
resect DNA 3’
of the
mismatch
Single base pair mismatch
on lagging strand
Single base pair mismatch
on leading strand
≥ 1 base pair insertion or deletionSingle base pair mismatch
Replication Resumes
PCNA
Mlh1 Pms2 (MutLα)
and
Msh2 Msh3 (MutSβ)
Polα
Exo1
Exo1
Polε
PCNA
Polα
Polδ/εPolα
PolεPolα
5’
3’
3’
5’
3’
5’
DNA Pol
DNA PolDNA Pol
Polδ
PolεPolαPolδ
Polδ Polδ
3’
5’
XPE
DNALigase
RPA
RPA
XPG
UV
DNA crosslinkers
Transcription coupled repairGlobal genome repair
Transcription
block
CSA
CSB
PARP1
HR23B
RNA Pol I/II
XPC
HR23BXPC
HR23BXPC
ERCC1
ERCC1
XPF
XPF
TFIID
TFB5XPBXPD
TFIID
TFB5XPBXPD
XPA
XPA
XPG
RPA
Polδ/εRFC
and
PCNA
Ubq
Ubq
Ubq
Ubq
P
PP
P P P P P
P
P
P P P P P
UBE2T
Chk1
DNA crosslinkers
Cisplatin
Mitomycin C
Fork stalling agents
Stalled
replication
fork
FANCD2/I
γ-H2AX
Repaired by NER
Histones
Repaired by HR
Translesion bypass
by Rev1/3
Translocation to
fork stall site
3’
3’
3’
5’
5’
5’
5’
ATRIP
ATR
FANCIFANCD2
ATRIP
ATR
FANCB
FA100
FANCL
FANCM
FANCGFANCA
FAAP24
FANCEFANCF
FANCC
FANCB
FA100
FANCL
FANCM
FANCGFANCA
FAAP24
FANCEFANCF
FANCC
E1 Ligase
REV1/3
REV1/3
Polδ/ε
Polδ/ε
3’
3’
3’
5’
5’
5’
5’
Polδ/ε
Mre11
Rad50
Nbs1
Rad51BCD
XRCC2/3
P
P
P
P
P
P
P
P
P
P
P
P
P
P P
P
P
BRCA2
BRCC36
BRCA2
BRCA1
Bard1
Ubc13Caesin Kinase2
BRCA1
ATM
Rad52
Rad52
Rad51BCD
XRCC2/3
BRCA2
BRCA1
Rad52
ATM
Ionizing radiation
Genotoxic chemicals
Free radicals
Mechanical stress
Homology search
and D-loop formation
Homologous chromosome
DNA synthesis
Rad51 monomers
histones
γ-H2AX
γ-H2AXH2A
Poly ubiquitinon H2A
3’
3’
3’
3’
3’
5’
5’
5’
5’
5’ 5’
5’
5’
3’
Resection
Resection
ATM
SMC1
Mre11
Rad50
Nbs1
ATM
MRN
complex
MRN
complex
Holliday
junction
resolvase
ATM
CtIP
CtIP
Rad54
Rad54
Rad51BCD
XRCC2/3
ATM
P P
P PP
Mdc1
DNA
Pol
DNA
Pol
Ligation and formation
of Holliday Junction
Non-crossover
(cut both junctions
vertically or horizontally)
Crossover
(cut both black or
both red arrows)
DNALigase1
Rap80
Abraxas
Rnf8
P
Mre11
Rad50
Nbs1
PP
P
P
P
P P
XRCC4 XRCC1
Artemis
ATM
DNALigase 3
ATM
Ionizing radiation
Genotoxic chemicals
Free radicals
Mechanical stress
Homologous
recombinations
Strand opening
and processing
by exonuclease
activity of WRN
No deletion
Accurate repair
DNA ligation
Hairpin DNA cut
by Artemis
Flap excision
Microhomology
mediated strand
annealing
Ku / Lig4 independent
pathway
Strand tethering
Hairpin DNA
ends
Ku / Lig4 dependent
pathway
Histones
Histones
γ-H2AX
H4K20
Me2
ATM
XLF
PNKP
Mre11
Rad50
Nbs1
ATM
Ku70
Ku80
Ku70
Ku80
P
P
DNA PKcs
DNA PKcs
Ku70
Ku80
Ku70
Ku80
P
P
DNA PKcs
DNA PKcs
Ku70
Ku80
Ku70
Ku80
DNALigase 4
Rag 1/2
Polβ
53BP1
WRN
Fen1
WRN
Short deletion
Inaccurate repair
PARP1
PARP1V JD
V JD
www.abcam.com/nuclearsignal
Copyright © 2009 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information/detail is correct at time of going to print. Produced in collaboration with James Haber and Farokh Dotiwala, Brandeis University.
DNA Repair PathwaysHomologous Recombination
Mismatch Repair
Featured DNA Repair antibodies
Nucleotide Excision Repair
DNA Strand Crosslink Repair
Nonhomologous End-Joining
ab29 PCNA antibody
Formaldehye fixedmouse tissue sectionsstained with ab29at 1/6000 dilution.
ab32138 PARP antibody
Paraffin-embeddedsections of human brainstained with ab32138 at1/25 dilution.
ab2888 ATM antibody
HeLa cells stained withab2888 at 1/200 dilution.
ab2893 gamma H2A.X
(phospho S139) antibody
Pachytene spermatocytesstained with ab2893at 1/200.
DA
PI
ph
os-A
TM
Merg
e
017-
01/0
9-P
S
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