dna structure and analysis activity 4.3: dye electrophoresis
TRANSCRIPT
DNA Structureand Analysis
Activity 4.3: Dye Electrophoresis
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Activity 4.3: Dye Electrophoresis
Research questions:– What charge do dyes have?– Are samples composed of multiple dyes?
Objectives:– Load and run dyes on an agarose gel– Determine the charge of each
electrophoresed dye– Determine the composition of each colored
sample
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Safety Reminders
Follow all laboratory safety procedures– Appropriate PPE should be worn at all times– Attention should be given when using electricity in the
presence of liquids. If there are leaks in equipment, notify teacher immediately
– Turn off power before removing lid and ensure that lid is in place before turning power on
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Skills to Master
Set up a horizontal gel electrophoresis chamber Load an agarose gel submerged in running buffer Perform agarose gel electrophoresis Program and utilize a power supply
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Running an Agarose Gel
Play video: Agarose Gel Electrophoresis
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Student Workstation Materials
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Protocol Highlights/Tips
If you are extracting dyes from candy, extract in 5% glycerol
Extract in the smallest amount of glycerol possible to ensure dyes are concentrated
If using food coloring make a 0.25% v/v solution using 5% glycerol as diluent
Ready-made loading dyes should be used at 1x concentration
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Summary
Make sure to:– Record all steps in your notebook– Ensure all lane contents are
recorded in an organized manner in your notebook
– Record the power supply settings and times in your notebook
– Place the micropipet tip just under the surface of the running buffer and deliver the dye into the well. Do not try to get the tip into the well. It could pierce the bottom of the well or break the side