1. Powdered sugar, its not just for doughnuts anymoreLLNL-PRES-642415This work was performed under the auspices of theU.S. Department of Energy by Lawrence LivermoreNational Laboratory under contract DE-AC52-07NA27344.Lawrence Livermore National Security, LLCGeorge Farquar, PhDIMAGE

2. Aerosol: suspension of fine solid particles or liquid droplets ina gas Biosecurity Track airborne contaminants Environmental effects Human health effectsLawrence Livermore National Laboratory LLNL-PRES-642415 3. DNA barcoded particles for aerosol transport studies and sourceattribution The simulant is an inert, non-living, non-viable, non-infectiousmaterial. It consist of two parts:1. Non-biological DNA molecules used for identification2. Sugar (a food-grade carbohydrateencapsulation material, i.e. Maltodextrin) 100 DNA base pairs allows for 1.6x1060 possible barcodes(Unlimited)Lawrence Livermore National Laboratory LLNL-PRES-6424153FDA approved food based material, found insweeteners and other products 4. As a result of the support received from DTRA and LLNL as a part of this testing,PFPA has been able to make marked performance improvements to the Pentagonsbiological detection systemJonathan Cofer, Deputy Director of PFPALawrence Livermore National Laboratory LLNL-PRES-642415 5. Testing occurred on November 2012 and February 2013 Primary objective was to determine the suitability of the new simulant as a tool for validation testing of aerosolsystems. Four releases occurred. Evaluating initial concept and a simultaneous release. PFPA sensors were triggered and all filters collected from DFUs and swabs were positive for the test material. PFPA models were evaluated and compared to experimental data.Lawrence Livermore National Laboratory LLNL-PRES-6424155Data collected with:Dry Filter Units (DFUs)Handheld particle counters(MET one)Aerodynamic Particle Sizer (APS)Spectrometer 6. DNATrax Safemade with sugar! Multiple releases can be conducted in a short timeperiod or simultaneously Post-testing cleanup is minimal Low burden for approval Tunable particle size Traditional Spores Cleanup required Difficulty obtaining approval forreleases Single release Barcoded Spores Difficult to receive approval to releasegenetically modified organisms Tracer chemicals (gases) Single releases Vastly different transport properties from1-10 micron particlesLawrence Livermore National Laboratory LLNL-PRES-642415 7. Catherine Elizondoelizondo1@llnl.govLawrence Livermore National Laboratory LLNL-PRES-642415 7