don ingle pond profile 2007 limnology 415 zooplankton & littoral macroinvertebrates brent beller...

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Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

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Page 1: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Don Ingle Pond Profile 2007Limnology 415

Zooplankton & Littoral Macroinvertebrates

Brent BellerHeather GangKaren JohnsonRandy Johnson

Page 2: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Outline

Introduction: Heather Methods: Brent Results: Karen Interpretation: Randy

Page 3: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Objectives

To determine fish sources of food present in pond Zooplankton

Identify zooplankton species present Determine densities of zooplankton at different depths and

locations Macroinvertebrates

Identify species present Compare population densities between East and West sides

of the pond. Fish

Analyze stomach contents to determine fish diet

Page 4: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Background Mr. Don Ingle’s pond, located south of Kendrick,

was built in 1995 and filled in 1996 Originally 14-16 feet (~4m) deep, currently

approximately 8 feet deep (2.5m) Deepest point near dam on Eastern side

Original area estimated at 2-3 acres Western third area of pond is shallower to attract

waterfowl

Page 5: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Background Continued… Original Fish Stocking

1500 Trout (500 in three class sizes) 50 breeding Bluegill 50 Largemouth Bass

Excellent trout fishing for next 4-5 years Successive Fish Stocking

500 more Trout 50 Smallmouth Bass 250 Catfish 250 Bullfrog tadpoles

Page 6: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Current Status Cattails have invaded

~100 dump truck loads have been removed to deepen the shoreline and remove cattails

Fishing has declined Bluegills have white worms

Page 7: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Methods

Yellow = Zooplankton

Red = Macroinvertebrate

Page 8: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Methods Macroinvertebrate

Samples Used D-net Two locations

Midway between East end and dock

Far West end Three 1.0 m passes Depth of approximately

0.3 m at each location Just above substrate

Page 9: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson
Page 10: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Methods

Macrovertebrate Identification Used Dissecting microscope Either counted entire sample or split sample into ¼ and

estimated total

Page 11: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Methods

Fish Samples Used stick seine net Three areas

East of dock At dock West of dock

Page 12: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Methods

Zooplankton Samples Used Schindler Trap Two locations

East End (Deep) 0.5, 1.5, 2.5 m

West End (Shallow) 0.5 m

Page 13: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Methods Zooplankton Identification/Counting

Added sample to dilution (50-85 ml) 2 ml sample from dilution added to counting plate Rotifers

Counted 2 squares Average #/square then multiply by 59 (# squares/plate)

Counted entire plate for other zooplankton Density (#/L) calculated using equations from previous

lab

Page 14: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Macroinvertebrate Results

Total Macroinvertebrate Taxa

0

100

200

300

400

500

600

700

800

900

Baetid

ae

Caenid

ae

Chiron

omida

e

Cerato

pogo

nidae

Chaob

orus

sp.

Nemato

da

Oligoc

haet

a

Ostroc

oda

Mea

n #

of

org

anis

ms

East

West

Page 15: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Macroinvertebrate Results There were more macroinvertebrates in the West than the East Oligochaeta were the dominant taxa with 846 counted Ostrocoda was second with 440 counted Little, if any, Baetidae, Caenidae, and Chaoborus sp. were found High variance between replicates

Page 16: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Fish Sampling Results

Fish sampling Only Young of the Year bluegill were found No fish were caught which were large enough to analyze

stomach contents

Page 17: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Zooplankton Results

Overview West 0.5 m had the highest mean zooplankton

density while East 2.5 m had the lowest Rotifers were the most numerous zooplankton Other zooplankton found included: Cyclopoids,

Ceriodaphnia, and Daphnia

Page 18: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Zooplankton Results

Mean Zooplankton Density

0

20

40

60

80

100

120

West 0.5m East 0.5m East 1.5m East 2.5m

Me

an

De

ns

ity

Page 19: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Zooplankton Results

There was approximately the same density of cyclopoids in all the East sites with East 2.5m having the lowest density

Daphnia and Ceriodaphnia had very low densities

Zooplankton Density (excluding Rotifers)

0

10

20

30

40

50

60

West 0.5m East 0.5m East 1.5m East 2.5m

Zo

op

lan

kto

n D

ensi

ty (

#/L

)

Cyclopoids

Daphnia

Ceriodaphnia

Page 20: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Zooplankton Results

Rotifers were found in the highest concentration at the deepest location (East 2.5m)

Rotifer Density

0

5000

10000

15000

20000

25000

West 0.5m East 0.5m East 1.5m East 2.5m

Ro

tife

r D

en

sit

y (

#/L

)

Page 21: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Interpretation

No adult fish were captured Small seine net Poor footing Inability to access deeper, preferred habitat Other methods possibly more effective

Electroshocking Gillnetting

Page 22: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Interpretation Macroinvetebrate Samples

Possible errors in sampling (dist. above substrate) and identification lead to very high variances

Time constraints Resulted in the inability to generate densities Oligochaeta were the most abundant within the

samples Low species diversity Most were found in the sediment and algae

Page 23: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Interpretation Zooplankton

Rotifers Very high densities observed within pond After dilution were still too numerous to accurately count Inaccurate estimation may have occurred due to uneven

dispersal on the counting plate Daphnia

Low densities observed <10/L

Page 24: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Interpretation High rotifer densities may be explained by a

trophic cascade effect Bluegill prey on daphnia Daphnia prey on rotifers High density of bluegill depresses daphnia,

allowing rotifer densities to increase

Page 25: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Interpretation

No significant differences of mean zooplankton density found between locations or depths at an alpha of 0.05

Page 26: Don Ingle Pond Profile 2007 Limnology 415 Zooplankton & Littoral Macroinvertebrates Brent Beller Heather Gang Karen Johnson Randy Johnson

Questions?