DosandDon’tsofRodentAsepticSurgery

MarcelPerret-GentilLaurieLongMitchMoore

Objectives• Toidentify&correctcommonmistakesthatleadtobreaksinaseptictechniquemadewhenperformingsurvivalrodentsurgery• IllustratevariouspracticalusesofPress’nSealtoperformasepticrodentsurgery• AlthoughPress’nSealisagroceryitem,itispackagedsterile• Alwaysplacestickysidetowardssurfaceintendedtobedraped.Non-stickysideshouldbeawayfromthissurface

• Theangelordeviliconsthroughoutthispresentationindicatewhetherthetechniqueiscorrectorwrong• 😇😇 =Correcttechnique• 👿👿 =Poortechnique

Nohaircover👿👿

Missinggownorlabcoat

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Nofacemask

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Non-sterilegloves

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Touchingnon-sterilesurface

Usingnon-sterilegloves👿👿

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Nohaircover👿👿

👿👿Nosurgicalattireatall

AllrequiredPPEisinplace:1. Gown2. Sterilegloves3. Facemask4. Haircover5. Armsleeves(notrequiredbut

highlyrecommended)

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Thisisthesurgerytable

Clippinghairwheresurgeryisgoingto

takeplace

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Thisiswhereyoucliphair,awayfromsurgerytabletoavoiddebrisfromfallinginthesurgicalwound Preparea

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Applyingapetroleumbasedproducttoprotecteyes

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Eyeprotection

Onlyophthalmicointmentshouldbeusedtoprotecteyeswhenananimalisanesthetized

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SterileSide Non-SterileSide

Sterileinstrumentsplacedonnon-sterilesurface

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Allinstrumentsareonsterilefield

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SterileSide Non-SterileSide

Drapingwithcottonmaterialleadstowickingoffluidsandbacteria

intothesurgicalwound

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ImpermeablematerialsuchasPress’nSealas

seenonthisratmakesforanidealdrape

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Overcrowdedhotbeadsterilizer

Toomanyinstrumentswillcooldownbeadsprecludingsterilizationofinstrumenttips

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Onlyafewinstrumentsshouldbeplacedinthe

hotbeadsterilizertoproperlysterilizeinstrumenttips

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Sutureandthumbforcepscrossingoverintonon-sterilefield

SterileSide

Non-SterileSide

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SterileSide

Non-SterileSide

Sutureandallinstrumentsarewithinthesterilefield

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Autoclavedpackageiscompromised– tornpackagerendersitems

non-sterile

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Autoclavedpackagehasnotbeencompromisedandall

contentsareconsideredsterile

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Post-oprecoverycageisdirty

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Post-oprecoverycageisclean,whichdecreasesthepotentialforpost-op

infections

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Therearemanydevilsinthispicture.Seeifyoucanidentifythembeforegoing

tothenextslide

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Herearethedevils:1. Notwearinghaircover2. Touchingcontaminatedobject

(glasses)withgloves3. Usingnon-sterile(nitrile)gloves4. Syringeouterpackageonsterile

field5. Tubeofophthalmicointment

onsterilefield6. Sutureouterpackageonsterile

field7. Anesthesiatube&animalnot

coveredwithsteriledrape8. Sterilegloveouterpackagingon

sterilefield9. Redtopbloodtubeonsterile

field10. Disinfectantbottleonsterile

field

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3👿👿2👿👿

8👿👿7👿👿10👿👿

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4👿👿5👿👿

1👿👿

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TheSoloSurgeon

• Frequentlyinvestigatorsassignsurgerydutiestoamemberoftheirlabwithoutadditionalhelp.Thelabmemberendsupperformingeveryaspectofthesurgeryalone• Sowhathappensifnon-sterileitemssuchastheanesthesiadial,stereotaxicapparatusknobs,lighthandlesandmicroscopedialshavetobemanipulatedduringsurgery?• Thenextslidesprovidepracticalsolutionstothe“SoloSurgeon”

Surgeontouchingnon-sterilepartswithsterilegloves

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CoveringtheanesthesiamachinedialswithPress’nSealorautoclavedaluminumfoilpreventscross-contaminationofthesurgicalwound

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CoveringpartsofthestereotaxicapparatusthatneedtobemanipulatedwithPress’nSealorautoclavedaluminumfoilpreventscross-contaminationofthesurgicalwound

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CoveringknobsofthesurgicalmicroscopewithPress’nSealorautoclavedaluminumfoil

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😇😇CoveringlighthandleswithPress’nSealorautoclavedaluminumfoil

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