Download - بكتريا عملي
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Lab Exercise 17:Biochemical Differentiation of some
Medically Important Gram-negative Bacilli
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MacConkey Agar
Left: Shigella
- result
Right: Escherichia
+ result
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Carbohydrate Fermentation TestsGlucose w/ Durham Fermentation Tube
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Carbohydrate Fermentation TestsSucrose Fermentation
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Triple Sugar Iron
-/+/+
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Indole Test
broth with tryptophan
IMViC Series
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Methyl Red TestIMViC Series
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Voges-Proskauer Test
αLeave uncapped for 15-20 minutes……..
IMViC Series
MRVP broth
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Citrate Utilization Test
- +
IMViC Series
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Urease Test
Slow positive
Negative result (acidic)
Uninoculated urea agar
Positive result (alkaline)
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Motility Test
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MacConkey agar
1. Which plate could have E. coli on it?
2. Which plate shows the presence of a lactose-fermenter?
3. Which plate could have Salmonella on it?
4. Which plate shows the presence of a lactose non-fermenter?
Answers:
1. A 3. B
2. A 4. B
A
B
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What is the name of the lab procedure demonstrated here?
Answer: Stabbing the butt!
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Use the chart on p. 181 and or the flow chart on p. 182 of your lab manual to figure out who I am!
I M V
C
U
Answer: Shigella sonnei
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I M V
C
U
Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
Answer: Escherichia coli
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I M V C
U
Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
Answer: Serratia marcesens
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Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
I M V C
Answer: Klebsiella pneumoniae
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Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
I M V C U
Answer: Salmonella typhimurium
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Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
I M V CU
Answer: Proteus vulgaris
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Use the chart on p. 181 and/or the flow chart on p. 182 of your lab manual to figure out who I am!
I M V C
Answer: Enterobacter aerogenes
U