Download - 284Determination of suitable method for DNA extraction from leaf tissue of jojoba shrub

7/23/2019 284Determination of suitable method for DNA extraction from leaf tissue of jojoba shrub

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3-Tyagi R.K. and Prakash S. 2004. Genotype-and sex-specific protocols for in vitro micropropagationandmedium-term conservation of jojoba. Biologia Plantarum. 48:19-23.

4-Mills D. Yanqing Z. and Benzioni A. 2004. Improvement of jojoba multiplication in vitro byventilation. In vitro Cell. Dev. Biol. Plant. 40: 396-402.

5-Saghari, Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard R.W. 1984. Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, choromosomal location, and population dynamics.

Proc Natl Acad Sci USA. 81: 8014-8018.

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Determination of suitable method for DNA extraction from leaf tissue of

jojoba shrub

Hosseini, F1*,**. Shahsavand Hassani, H2. Arvin, M. J2,3, Mohammadinejad, Gh2. Baghizade, A3.1Agricultural Biotechnology MS.c student of Shahid Bahonar Univercity of Kerman, Kerman, Iran; 2college of

agriculture, Shahid Bahonar Univercity of Kerman, Kerman, Iran; 3International Center for Science, High Technologyand Environmental Science, Kerman, Iran

AbstractJojoba is an evergreen and dioecious shrub, that commercially valuable for it

,s seed. The seed containing a

characteristic liquid wax of economic importance in industry, nutrition and medicine (e.g. cosmetics,

anticancer compounds). In recent years, the breeding and biotechnological programs were expand on thisshrub. The extraction of DNA is the first step for such programs. In this study, the optimum method

investigated for genomic DNA extraction, among of two modified CTAB and dellaporta methodes, ttest to

be shown that suitable method for genomic DNA extraction from jojoba leaf is CTAB.

Key words:CTAB, Jojoba, Dellaporta method, Ttest, DNA extraction

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Download - 284Determination of suitable method for DNA extraction from leaf tissue of jojoba shrub

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