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NEPHELOMETRY AND
TURBIDIMETRYBYDr. Suman PattanayakAssociate ProfessorDepartment of Pharma Analysis & QA.
Vijaya Institute of Pharmaceutical Sciences for Women
M. Pharm/ I SemAdvance Pharmaceutical Analysis
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Presented By –Mr. Shaise JacobFacultyNirmala College of Pharmacy,MuvattupuzhaKerala, IndiaEmail - [email protected]
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INTRODUCTION
• When electromagnetic radiation (light) strikes a particle in solution, some of the light will be absorbed by the particle, some will be transmitted through the solution and some of the light will be scattered or reflected.
• The amount of light scattered is proportional to the concentration of insoluble particle. We will focus on the concept of light scatter
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THEORY
Scattered light may be measured by• Turbidimetry• Nephelometry• In turbidimetry, the intensity of light transmitted
through the medium, the unscattered light, is measured.
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• Turbidometric measurements are made at 180o from the incident light beam.
• In Nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.
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• The two techniques differs only in the manner of measuring the scattered radiation.
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• Turbidity can be measured on most routine analysers by a spectrophotometer (absorbed light)
• Reduced sensitivity and precision. • Extent of light scattering increases as wavelength
increases • The intensity of scattered light is normally measured
by Nephelometer.
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• Light scattering is the physical phenomenon resulting from the interaction of light with a particles in solution.
Dependent on :• Particle size• Wavelength• Distance of observation,• Concentration of particles• MW of particles
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Tyndall Effect
• Scattering of light- by particles in a colloid or suspension.
• the longer-wavelength light is more transmitted while the shorter-wavelength light is more reflected via scattering.
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Light Scattering Phenomenon
• The blue color of the sky and the red color of the sun at sunset result from scattering of light of small dust particles, H2O molecules and other gases in the atmosphere.
• The efficiency with which light is scattered depends on its wavelength, λ. The sky is blue because violet and blue light are scattered to a greater extent than other longer wavelengths.
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• NEPHELOMETRY• ↓ concentration, uniform scattering, intensity of
scatted light proportional to conc. measured at 900
• TURBIDIMETRY• ↑ concentration, scattering not uniform, intensity of
transmitted light measured at 1800
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Turbidimetry↨
Colorimetry• Measurement of the intensity of light transmitted
through a medium, light intensity is decreased.Nephelometry
↨Fluorimetry
• Measurement of scattered light at 900
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COLORIMETERSimilarity: transmitted light measured measured at 1800
TURBIDIMETERSimilarity:transmitted light measured measured at 1800
Difference: Absorption of radiation
Difference: Scattering of radiation
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FLOURIMETRYSimilarity: Emergent radiation measured at 900
Difference:» emitted radiation measured» emitted radiation – longer WL than incident light
NEPHELOMETRYSimilarity: Emergent radiation measured at 900
Difference:» Scattered radiation measured» emitted radiation – same WL as that of incident light
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CHOICE OF THE METHOD
• depends upon the amount of light scattered by suspended particles present in solution.
• TURBIDIMETRY - high concentrated suspensions• NEPHELOMETRY - low concentrated suspensions - more accurate results
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INSTRUMENTATION
• The basic instrument contains• Light Source:Tungsten lamp, White light - nephelometers • Filters - Turbidimeter (blue filter or 530 nm) Nephelometer (visible filter)• Sample cells• Detectors (photometric)
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• CELLS• cylindrical cells - flat faces to minimize reflections &
multiple scatterings
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FACTORS AFFECTING MEASUREMENTS
• The amount of radiation removed or deviated from the primary radiation beam depends on the following factors
1.Concentration• Turbidimetry: S = log I/Io= kbc• T=Transmittance = I/Io• S = turbidence due to scattering• k = turbidity constant• b= path length• c = concentration of suspended material
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• Nephelometry:• Is = Ks Io C• Is = scattered intensity• Ks= empirical constant• Io = Incident intensity• c = concentration of suspended material
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2. Effect of Particle Size on Scattering• Size and the shape of the particles responsible for
the scattering.• Because most analytical applications involve the
generation of a colloidally dispersed phase in a solution, those variables that influence particle size during precipitation also affect both turbidimetric and nephelometric measurements.
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• Turbidimetry-Practical Considerations• Selecting λ: Important. It is necessary to avoid
radiation that is absorbed by the sample.Sample Preparation• Scattering is related to:• 1.Concentration of the scattering particles• 2.Particle size• 3.Particle shape
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APPLICATIONS
1. Analysis of water clarity, conc. of ions2. Determination of CO2
3. Determination of inorganic substances Sulphate – barium chloride Ammonia – Nesslers reagent Phosphorus – Strychine molybedate4. Biochemical Analysis5. Quantitative Analysis – (ppm level)
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6. Miscellaneous Water treatment plants, sewage work, refineries, paper
industry7. Atmospheric pollution smokes & fogs8. Determination of mole. Wt of high polymers9. Phase titration
NEPHLOTURBIDIMETER Two detectors
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