6- Microbial Nutrition & Growth
Bacterial growth
electron source lithotrophs (inorganic)
organotrophs (organic)
CHNOPS
Singlet oxygen
Toxic forms of oxygen
contains O2 2- (peroxide anion)
Detoxification enzymes
Other antioxidants: carotenoids, glutathione, Vit E & C, etc.
Micro-aerophiles
Nitrogen cycle
Fig 26.17
acidophiles
basophiles/ alkalinophiles
Vibrio
Ferroplasma
neutrophiles
Fig. 2.14
Halophiles
obligate
tolerant
Barophiles
Extremophiles
Ecological associations
Antagonistic
Synergistic
Symbiotic
Biofilms
Quorum sensing
Other kinds of samples:
food, water, air, soil, etc.
Streak plate
Pour plate
Spread plate
Inoculating needle & loop
pipet & pipetters
micropipette = micropipet = micropipetter
Disposable tip
tubes
Petri plate
flask
containers
For beer
agar broth agar
slant deep
agar
(for Nutrient Broth: just leave out the agar)
General medium Selective medium
(nutrient agar) (MacConkey agar)
Differential medium
(MacConkey agar)
Differential media (newer)
candle jar
capnophilics
Anaerobic jar Glove box
Laminar flow hood
(for aerobes)
Standard freezer -20oC
Ultra deep freezer -70 to -86oC
Liquid nitrogen -196oC
refrigerator
Lyophilization = freeze-drying
Other: dessication, crystallization
binary fission
budding
fragmentation
Growth curve
Plate count using serial dilutions on spread plates
Colony counter
Cell counter
MPN
26 cells /100 ml
(from T6.6)
Most Probable Number
dry weight
Indirect methods
metabolic activity
Turbidity
spectrophotometer