Download - 8 connective tissue
![Page 1: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/1.jpg)
Connective Tissue
DR. KIRTI SOLANKE
![Page 2: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/2.jpg)
Connective Tissue• Found everywhere
• Most abundant
• Development
• Functions– Protection– Support– Bind other tissues– Energy storage/insulation– Hormone production
![Page 3: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/3.jpg)
COMPONENTS
LIVING NON - LIVING
CELLS MATRIX
FIXED WANDERING
FIBROBLASTS
FAT CELLS
PERSISTANT MESENCHMAL CELLS
MACROPHAGES
MAST CELLS
PLASMA CELLS
PIGMENT CELLS
NEUTROPHILS
EOSINOPHILS
FIBRES GRD SUBS
COLLAGEN
ELASTIN
RETICULAR
MPS
SO4
NON-SO4
GLYCO PR
![Page 4: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/4.jpg)
Fibers
• Collagen/ white fibers
• Elastic/yellow fibers
• Reticular fibers/Argyrophilic – fine collagen fibers
![Page 5: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/5.jpg)
COLLAGEN FIBRES
• In bundles branch,1-12um in dia, White• H &E and Van Gieson:pink;masson’s
T:blue;• Tensile force,birefringence,swell with weak
alkali,boiling convert it into gelatin.• Synthesis:fibroblast,regulation,degradation(
MMP)• Made of tropocollagen mol;made of 3
polypeptide chains(procollagen)
![Page 6: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/6.jpg)
![Page 7: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/7.jpg)
![Page 8: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/8.jpg)
SYNTHESIS
• AA taken up by cells& linked PROα CHAINS αchain 3chains join to form PROCOLLAGEN MOL such mol leave cell through secretory vacuoles to form TROPOCOLLAGEN MOL aggregate to form COLLAGEN FIBRILS.(vit C,oxy)
• Fibrillogenesis
![Page 9: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/9.jpg)
TYPESTYPE LOCATION
I(250nm dia) SKIN,BONE,TENDON,FASCIA,CAPSULE
II(20 to 100nm) HYALINE CARTILAGE ,NOTOCHORD,INTERVERTEBRAL DISC
III RETICULAR FIBRES,FETAL SKIN,BLOOD VESSEL.
IV BASAL LAMINA,KIDNEY GLOMERULI
![Page 10: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/10.jpg)
![Page 11: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/11.jpg)
RETICULAR FIBRES(Argyrophilic)
• Collagen type III,Striation(68ηm),20ηm diameter,do not bundle,uneven in thickness.
• Form network by branching• Silver impregnation:black but type I:brown • H&E:not identified;• More carbohydrates:PAS• Early mechanicalstrenth,delicate,suporting
stroma in lymphatic T.(not thymus)• Synthesis:reticular cells
![Page 12: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/12.jpg)
RETICULAR FIBRES
![Page 13: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/13.jpg)
![Page 14: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/14.jpg)
ELASTIC FIBRES
• Run singly,branches,0.1-0.2μm in dia• Not well stained H&E;Certain fixative make
them refractile then can be visualised• Composed of:central core of elastin &
surrounding network of fibrillin microfibril• Lacks hydroxylysine,random distribution of
glysine:HYDROPHOBIC & random coiling.• Vertebral ligaments,larynx,elastic A
![Page 15: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/15.jpg)
WEIGERT’S STAIN
![Page 16: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/16.jpg)
![Page 17: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/17.jpg)
![Page 18: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/18.jpg)
GROUND SUBSTANCE
• Glycoprotiens:keratan s
• Multiadhesive glycoproteins:laminin,fibronectin
• Proteoglycans:aggrecan,decorin
Pr + long chain polysaccharide –glycosoaminoglycans (MPS)
» Sulphated» Non sulphated
![Page 19: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/19.jpg)
Ground Substance
• GSG linked with pr.
• Carry sulphate gr(so3-)Carboxyl gr(coo-).
• Thus proteoglycans r in long chain,
• Can retain water thus proteoglycans form
• semi-solid, gel:stiffness
• Molecular arrangement: sieve
• Barrier:kidney;gas exchange:lungs
![Page 20: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/20.jpg)
![Page 21: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/21.jpg)
GLYCOSAMINOGLYCANS
TISSUE CHONDROITIN SULPHATE
DERMATAN SULPHATE
HEPARAN SULPHATE
HEPARIN
KERATAN S.
HYALURNIC ACID
TYPICAL CT
+ +
CARTILAGE
+ + +
BONE +
SKIN + + + +
BASEMENT M
+
OTHERS B/V LUNGS MAST C CORNEAINTER V DISC
SYNOVIAL FLUID
![Page 22: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/22.jpg)
HYALURONIC ACID
• Hyaluronan:free carbohydrate chain
• Polymers r very large
• Synthesized by enzymes ¬ posttranslatioally modified
• No sulfate,proteoglycan aggregates
• So cartilage resist compression without inhibiting flexibility
![Page 23: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/23.jpg)
CELLS
• FIXED TYPE:Fibroblast,Persistant mesenchymal cells,Adipocytes.
• WANDERING CELLS:Lymphocyte,Monocyte,Mast cell,Macrophages,Neutrphil,Plasma cell,Eosinophils.
![Page 24: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/24.jpg)
![Page 25: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/25.jpg)
FIBROBLAST ADIPOCYTE
![Page 26: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/26.jpg)
MACROPHAGE
![Page 27: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/27.jpg)
MAST CELL
![Page 28: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/28.jpg)
LYMPHOCYTE
![Page 29: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/29.jpg)
PLASMA CELL
![Page 30: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/30.jpg)
![Page 31: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/31.jpg)
CLASSFICATION OF C. T.
• Types of cells
• Types of fibres
• Amount of ground subs
![Page 32: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/32.jpg)
CLASSIFICATION
CONNECTIVE TISSUE
ADULT C. T. FETAL C. T.
ORDINARY SPECIALISED
BLOOD
CARTILAGE
BONE
LOOSE C. T. DENSE C.T.
WHITE YELLOWAREOLAR
ADIPOSE
RETICULAR
REGULAR IRREGULAR
TENDON
LIGAMENT
APONEUROSIS
S/C TISSUE
![Page 33: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/33.jpg)
FETAL C.T:WARTON’JELLY
![Page 34: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/34.jpg)
LOOSE AREOLAR T.
![Page 35: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/35.jpg)
![Page 36: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/36.jpg)
![Page 37: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/37.jpg)
Loose Connective• Areolar Tissue
– Gel like matrix – Fibroblasts, mast cells– Collagen, elastic and reticular fibers– Functions to wrap and cushion organs– Found in the lamina propria, around organs,
capillaries
![Page 38: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/38.jpg)
Dense Connective
• Dense Regular– Parallel collagen fibers
with a few fibroblasts and a few elastin fibers
– Attach muscles to bones
– Great tensile strength in one direction
– Tendons and ligaments
![Page 39: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/39.jpg)
DENCE REGULAR C.T.
![Page 40: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/40.jpg)
TENDON
![Page 41: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/41.jpg)
Dense Connective
• Dense irregular– Collagen fibers with a few elastic fibers
haphazardly arranged– Strong in many directions– Dermis, joint capsules, submucosa of
digestive tract
![Page 42: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/42.jpg)
DENCE IRREGULAR C.T.
![Page 43: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/43.jpg)
ADIPOSE TISSUE
![Page 44: 8 connective tissue](https://reader036.vdocument.in/reader036/viewer/2022062616/549660a9b4795920778b4759/html5/thumbnails/44.jpg)
APPLIED
• SCURVY:Vit C DEFICIENCY
• OSEOGENESIS IMPERFECTA:Brittle bone disease,blue sclera hearing loss,TYPE I asso
• EHLERS-DANLOS :hypermobility of joints of digit,TYPE III asso
• MARFAN’S S:FBN1,fibrillin gene