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About me…
Major: Environmental Science (B.S.)
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About Dr. Nienow… He’s great!
Exper:se: Ecophysiology and systema:cs of algae Affilia:on: Valdosta State University, Biology Department BA/MA Mathema:cs, UC San Diego Ph.D. (Microbial Ecology) – Florida State University
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Diatoms � Generally symmetrical � Silicious frustules � Phytoplankton (algae) � Producers � 45% global oceanic produc:on
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‘Norm’
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‘Norm’
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‘Norm’
Models
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Methods of sampling…
Discrete whole water samples (using CTD))
Vertical Plankton Tows
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Light microscope
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Pigment chromatography (for HPLC)
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Imaging flow cytometry (FlowCAM)
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Scanning Electron Microscope
SEM
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What I did… � A little bit of everything! � Preparing samples � Analyzing samples, mainly FlowCAM � Synthesizing data � Capturing samples (cruise!)
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December 2011
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Metadata
Lots of data! CTD backlog
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• How to prepare samples for SEM analysis • How to navigate and take photos on SEM • How to prepare samples for FlowCAM analysis • How to use FlowCAM • How to prepare slides for light microscope • Experience on light microscope • How to prepare samples for HPLC analysis • How to use the HPLC machine • Experience on the field (9 days at sea) • Learned phytoplankton taxonomy (diatoms at the genera level) • Always keep a journal of what you’re doing
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Acknowledgements NOAA-‐NGI Diver$ity Intern$hip Program
• Dr. Nienow • Courtney M. Bryller • Dr. Miller-‐Way • Natalie Ortell Cumbaa
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Ques:ons?