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Role of AC3 on Geminiviral Replication
-A journey through past.
Kalyan Kumar Pasumarthy
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WT TGMV-M_AC3-1 Normal Elmer et al., NAR (1988), 16: 7043-60
AC3 Mutation in TGMV
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TGMV-AC3 has role in infectivity and symptom
formationElmer et al., NAR (1988), 16: 7043-60
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1. 2AX2B plant
2. AL1AL2AL3X 2B plant
3. 2A plant
4. 2B plant
5. AL1 plant6. AL2 plant
7. AL1 X2B plant
8. AL2 X2B plant
9. AL3 X2B plant
10. AL1AL2 X2B plant
11. AL1AL3 X2B plant
12. ds supercoiled DNAB
Nicotiana tabacum cv. Samsun
AC3 does not have significant effect on replication of DNA B
Southern blot of transgenic plants with TGMV DNAB as probe
Hayes, RJ., et al., (1989), NAR 17:10213-22
Transgenic studies in TGMV
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AC3 C( AC3
Sunter et al: Virology(1990), 179: 69
TGMV-AC3 Mutation study in protoplasts
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Sunter et al: Virology(1990), 179: 69
Replication of DNA-A Replication of DNA-B
AC3 mutation reduces replication of DNA A & B by 50 fold in
N.tabacum protoplasts
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TGMV
AC3
Transgenics Protoplasts
No significant change
in replication
Drastic reduction in
replication (~ 50 fold)
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Etessami et al., JGV (1991), 72:1005-12
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The progeny of mutant pCLVAC 3-2 were investigated by Southern blot analysis (Fig. 5). For the
purpose of this particular experiment, leaf material was selected that showed similar (mild)
symptoms. For this reason the wild-type and mutant samples contained comparableamounts of viral DN A. The ssDNA and scDNA forms of both genomic components were
produced in the same relative abundance in plants infected with the AC3 mutant and
wild-type virus (compare lanes 3 and 4).
W W W W
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AC3 : 134 Aa M t_AC3 : 122Aa
Morris et al: JGV (1991), 72: 1205
ACMV m
t_AC3
ACMV AC3
ACMV m
t_AC3
ACMV
AC3 Mutation in ACMV
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Morris et al: JGV (1991) 72: 1205
DNA-B transgenic plants agro-infiltrated with AC3 mutant DNA-A
ACMV-AC3 mutation lead to delay and amelioration of disease
symptoms and correleated with significant reduction in DNA-B
replication
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AC3 Mutation in BCTV (california strain)
Stanley et al.,. Virology(1992) 191:396-405
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Plants were observed for symptoms after1 week in Beta vulgaris and3days later in Nicotiana benthamiana
Stanley et al.,. Virology(1992), 191:396-405
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Stanley et al.,. Virology(1992), 191:396-405
The data suggests the type of plant system (natural host/
permissive host) governs the role of AC3 on viral genome
replication
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ACMV AC3
ACMV m t_AC3
ACMV AC3
ACMV m t_AC3
Hormuzdi et al, Virology (1995) 206:1044
AC3 : 136 Aa M t_AC3 : 127Aa
AC3 Mutation in BCTV (logan strain)
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Hormuzdi et al, Virology (1995) 206: 1044
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Hormuzdi et al, Virology (1995) 206: 1044
AC3 of BCTV (Logan strain) has no significant effect on
the viral replication even in host plant
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Hormuzdi et al, Virology (1995) 206: 1044
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Hormuzdi et al, Virology (1995) 206: 1044
However, when the similar experiment was performed in
tobacco protoplasts, replication was reduced
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Hormuzdi et al, Virology (1995) 206: 1044
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Role of AC3 in PYMV Replication Leaf Disc Assay
In leaf discs, AC3 mutation reduces the viral DNA
replication
Sung et al., JGV (1995)76:1773
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AC3Heterogeno s
Activity
Vir s to vir sTGMV and PYMV AC3 enhances DNAA
ACMV AC3 has no effect on DNAA
Strain to strainBCTVAC3(california) enhances replication
BCTVAC3(logan) does not effect replication
Host to ostBCTVAC3 (california) enhances viral replicationin S garbeet and has no effect in Tobacco
System to systemAC3 enhances replication in Protoplast
No effect on replication in Transgenic/Agroinfiltration
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