![Page 1: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/1.jpg)
Agricultural By-Products for Shelf
Life Extension
Prof Graham Bonwick
Institute for Agri-Food Research & Innovation
Newcastle University
![Page 2: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/2.jpg)
Packaging – Safety, Security & Sustainability
• Population growth and resource pressures – waste reduction
• Supply chains longer and more complex – integrity & fraud
• Consumers time-poor, disconnected and less ‘food safety aware’ - information
![Page 3: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/3.jpg)
Packaging in the Media
Macro, micro and nano scale plastic impacts
![Page 4: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/4.jpg)
Packaging & Consumers
• Wide range of industry responses: -‘Plastic free aisles’ /
• X% reduction by 20?? / single polymer use / recycled polymers /
• No plastic
• Packaging seen by the consumer as a problem, not a solution
• Perceptions influence purchasing activities
![Page 5: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/5.jpg)
Drivers - Legislation
EUROPEAN COMMISSIONBrussels, 2.12.2015COM(2015) 614 finalCOMMUNICATION FROM THE COMMISSION TO THE EUROPEAN PARLIAMENT, THECOUNCIL, THE EUROPEAN ECONOMIC AND SOCIAL COMMITTEE AND THE COMMITTEE OF THE REGIONSClosing the loop - An EU action plan for the Circular Economy
![Page 6: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/6.jpg)
Natural Solutions – Agri-Food By-Products
Tomato leaf and recycled cardboard pulp
![Page 7: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/7.jpg)
Natural Materials - Chitosan
• Traditionally derived from processing crustacean by-products
• Has various applications due to its biodegradable and nontoxic properties
• Chitosan nanoparticles are found to be more effective against pathogens
• High surface charge density – microbial membrane damage
![Page 8: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/8.jpg)
Circular Bioeconomy – Growth Opportunities
![Page 9: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/9.jpg)
Electrospinning
Polystyrene nanofibres – 85% open space, antimicrobial
• A simple and versatile manufacturing procedure compared with more complex nanostructure assembly methods.
• Applications to sensors, surface coatings, films
![Page 10: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/10.jpg)
Structured Nanofibres
• Can be produced with co-spun encapsulated materials for controlled release
Nisin – polycyclic peptide
from Lactococcus lactis.
Effective against a wide range
of Gram positive species
including the food pathogen
Listeria monocytogenes
![Page 11: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/11.jpg)
Agri-Food By-Product Valorisation
• Agri-food by-products for production of nanofibers for food films and
packaging coatings
• Chemical and enzyme treatment of by-products to enhance solubility for
electrospinning
• Treatment to enhance porosity for bioactive release e.g. polyphenols
Milled tomato by-product treated to increase porosity
![Page 12: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/12.jpg)
Agri-food waste for nanofiber films
Nanofiber base materials - alginate, chitosan, PEO
![Page 13: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/13.jpg)
Control – uncoated Al foil diskNo inhibition of E. coli growth
Agri-food waste for nanofiber films
Active packaging film –E. coli growth inhibition by tomato material suitable for bioactive release embedded in a food grade edible alginate film.
![Page 14: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/14.jpg)
E. coli growth inhibition – tomato skin processing
Inhibition of E.coli K12 growth on Mueller-Hinton agar inoculated at 106 CFU/ml by
PEO (6.8% w/w) / chitosan (0.8% w/w) nanofiber film with added tomato. Values are
means ± s.d., n=4. T1b: undigested dry tomato skin; T2b: Dry digested (24h) tomato skin;
T3: Dry digested (48h) tomato skin; T4b: undigested wet tomato skin; T5b: wet digested
(24h) tomato skin; T6b: wet digested (48h) tomato skin; DIb: (PEO 6.8% w/w, chitosan
0.8% w/w and distilled water).
0
5
10
15
20
25
30
35
40
T1b T2b T3 T4b T5b T6b DIb
Inhib
iton Z
one (
mm
^2)
Gram Negative
![Page 15: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/15.jpg)
B. subtilis growth inhibition – tomato skin processing
0
2
4
6
8
10
12
14
T1b T2b T3b T4b T5b T6b DIb
Inhib
iton Z
one (
mm
^2)
Inhibition of B. subtilis growth on Mueller-Hinton agar inoculated at 106 CFU/ml by PEO (6%
w/w) / alginate (1.5% w/w) nanofiber film with added tomato. Values are means ± s.d., n=4.
T1b: undigested dry tomato skin; T2b: Dry digested (24h) tomato skin; T3b: Dry digested
(48h) tomato skin; T4b: undigested wet tomato skin; T5b: wet digested (24h) tomato skin;
T6b: wet digested (48h) tomato skin; DIb: (PEO 6.8% w/w, chitosan 0.8% w/w and distilled
water).
Gram Positive
![Page 16: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/16.jpg)
B. subtilis growth inhibition – blend and inclusion
0
2
4
6
8
10
12
14
16
18
20
DIb H4b T1b SF8 SF9
Inhib
iton Z
one (
mm
^2)
Inhibition of B. subtilis growth on Mueller-Hinton agar inoculated at 106 CFU/ml. Values are
means ± s.d., n=4. DIb: (PEO 6.8% w/w, chitosan 0.8% w/w and distilled water); H4b:
(PEO 6.8% w/w, chitosan 0.8% w/w and wheat stem); T1b: (PEO 6.8% w /w, chitosan 0.8%
w/w and tomato skin); SF8: (PEO 6% w/w, alginate 1.5% w/w); SF9: (PEO 6% w/w, alginate
1.5% w/w and tomato skin).
Gram Positive
![Page 17: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/17.jpg)
Proof of Concept - Next Steps
• Micronised cereal by-products as nanofiber feedstock
• Greener by-product processing – enzymes instead of chemicals
• Performance testing – anti-microbial and anti-oxidant properties
• Formulation modifications – electrospinning performance and film characteristics
• Shelf life extension – real foods packaged in nanofiber films
![Page 18: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/18.jpg)
Agri-Food Waste Packaging – Safety
• Migration of contaminants and allergens
• Toxicity (in vitro / cytotoxicity)
• Biodegradation
• Consumer acceptance / perceptions
Collaborative research through IAFRI– 3 jointly funded PhD projects selected for 2018
![Page 19: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/19.jpg)
Collaboration with Fera Science Ltd
The UK National Reference Laboratory (NRL) for Materials and
Articles in Contact with Food
Agri-Food Waste Packaging Safety
Collaborative research through IAFRI– 3 jointly funded PhD projects selected for 2018
![Page 20: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/20.jpg)
Non-targeted analysis
Non-targeted analysis
Volatile substances
Trace elements
Non-volatile
and polar substances
Semi-volatile
substances
![Page 21: Agricultural By-Products for Shelf Life ... - Packaged Summit](https://reader034.vdocument.in/reader034/viewer/2022042421/6260685efecca41d5b4b1311/html5/thumbnails/21.jpg)
Biopolymer Packaging
Another opportunity to positively influence the customer?
Thank you for your attention!
September 2018
Postgraduate Certificate Food Packaging
FuturePack meeting – Fera Science Ltd, York