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Antigen PresentationFundamentals I for Dentistry & Optometry
Textbook:Medical Microbiology, 6th Edition, Murray
Chapter 11
Jim Collawn, MCLM [email protected]
934-1002
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Study Objectives1. Compare and contrast class I and class II
MHC-restricted responses with regard toA. source of antigensB. antigen processing requirementsC. role of chaperonesD. types of T cell involved
2. Discuss the invariant chain’s role in the demarcation between class I and class II MHC-restricted responses.
3. Discuss HLA-DM’s possible role in antigen processing.
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Humoral and Cell-
mediated Immune
Responses
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Major Players in T cell Responses
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T lymphocyte and Macrophage (right)
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Types of T cells1. Cytotoxic T cells (CTLs)
A. Kill virally infected cellsB. Kill cells containing cytosolic
bacteriaC. Kill tumor cells
2. Inflammatory T cells (TH1)A. Activate macrophages to kill intracellular bacteria
3. Helper T cells (TH2)A. Activate B cells to make antibody
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Schematic Diagrams of MHC class I and class II Molecules
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MHC class I (top view)
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Major Histocompatibility Complex
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HLA-DR1 (blue) and HLA-A2
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MHC class I
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MHC class II
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Anchor Residues for MHC class I peptides
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Conformation of Peptides Bound to MHC class I
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Solvent-accessible Area of H-2Kb
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Anchor Residues for MHC class II peptides
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Antigen Processing is Necessary for Helper T cell Activation
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Antigen-presenting Cells(MHC Class II-positive cells)
HLA-DR, HLA-DP, HLA-DQ
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Target Cells(MHC Class I-positive cells)
HLA-A, HLA-B, HLA-C
All nucleated cells
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Degradation of Intracellular Proteins
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Transporter associated with Antigen Processing
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Generation of Peptide-class I MHC Complexes
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Demarcation between MHC
class I and class II
Processing Pathways
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Cytosolic and Endocytic Pathways for Antigen Processing
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Assembly of MHC class II Molecules
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Study Objectives1. Compare and contrast class I and class II
MHC-restrictedresponses with regard to
A. source of antigensB. antigen processing requirementsC. role of chaperonesD. types of T cell involved
2. Discuss the invariant chain’s role in the demarcation between class I and class II MHC-restricted responses.
3. Discuss HLA-DM’s possible role in antigen processing.
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Sample Questions• The MHC class II molecule
– is associated with ß-2 microglobulin.– binds peptide antigens from the endogenous pathway.– is associated with the invariant chain.– is expressed on all cell types.
• • Exogenous antigens
– are taken up by cells through endocytosis or phagocytosis.– are bound to MHC class I molecules.– are processed by the proteasome.– become associated with MHC class I molecules in the endoplasmic
reticulum.•
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Sample Questions• HLA-DR
– presents antigens to cytotoxic T cells.– is a chaperone that facilitates CLIP removal and peptide binding.– is an MHC class II molecule.– binds to endogenous antigens.
• • The proteolytic activity necessary for the generation of MHC class II-
associated peptides is provided by– the proteasome.– the lysosome.– furin.– the endoplasmic reticulum.