Download - Arabidopsis Thaliana Gene AT5G58610
Arabidopsis Thaliana Gene
AT5G58610
Nancy Gallardo
HC70AL
What Protein Does AT5G58610 Code for?
• Regulates gene expression
• 1065 amino acids
PHD-Type Zinc Finger Transcription Factor
What's Around Gene AT5G58610 ?
AT5G8610 AT5G58620AT5G58600
AT5G58600
3' 5' 3' 5' 5' 3'
5' 3' 5' 3' 3' 5'
1045bp 2286bp
23703997-23708279
AT5G58620
5'
5'3'
3'
What is the Structure of AT5G58610 ?
Exon Exon Exon Exon Exon Exon Exon Exon
3'
5'
5'
3'
Chromosome 5
4283 base pairs
Reverse Orientation
9 Exons and 8 Introns
Exon
Expected T-DNA InsertionSalk_041367
Intron 2 Intron 1
T-DNA
546bp 362bp
Exon 1 Fw- 20bp
Rv- 20bp
LBb1-150bpExpected Sizes:WT= 908bpT-DNA= 696bp
3' 5'
What Were the Genotyping Results ?
PCR Genotyping AT5G58610
WT= 908 T-DNA=696
700bp
1% agarose, 105 Volts, 04/15/2008, 1.5Hour Run
Do these results agree with the RT-PCR results?
Where is AT5G58610 Active?
RT-PCR AT5G58610
1% Agarose 1 Hour Run 70 Volts 04/24/2008
Arabidopsis Thaliana Gene
AT4G33280
What Protein Does My Gene Code for?
B3-Domain Transcription Factor Families
Regulate gene expression 3 families in Arabidopsis Thaliana 337 amino acids
What's Around Gene AT4G33280 ?
AT4G33280 AT4G33290AT4G33270
3' 3' 5'
5' 5' 3'
561bp 217bp
16047358-16049359
5'
3' 5'
3'
What is the Structure of AT4G33280?
Exon Exon Exon Exon Exon Intron Intron Intron Intron
UTR UTR
•Chromosome 4
•2002 Base pairs
• Reverse Orientation
•5 Exons, 4 Introns, 2 UTRs
3'
5'
5'
3'
Expected T-DNA Insertion
Exon 5
AT4G32270 Intron 4
T-DNA
260Bp 142bp
LBb1-150bp
Fw-23bp
Rv-20bp
3'
Salk_072801
Expected Sizes:WT= 402bpT-DNA= 292bp
5'
What Were the Plants' Genotypes ?
Expected T-DNA=292bp Expected Wild Type=402bp
PCR Genotyping AT4G33280
Wild Type= 25
Homo T-DNA= 0
Hemi T-DNA= 0
1% agarose1 Hour 05/15/2008105 Volts
Carrying Out More Genotyping
PCR Genotyping AT4G33280
1% agarose05/29/081Hr. Run105 Volts
Genotyping With Controls
PCR Genotyping of Plants: 7, 17, 26, 33
1% Gel 05/30/08 1 Hr.Run 105 Volts
AT4G33280's Activity
RT-PCR AT4G33280
Expected Product= 254bp
5:34-7:12 pm1.5% Agarose05/20/2008105 Volts
Cloning the Upstream Region
Colony Digestion
pENTR+ Gene Vector
Promoter
Non-Recombinant 2580bp 262bp
Recombinant 2842bp 1% Agarose, 1 Hour Run, 1% agarose, 105
Volts
Nomarski
THANKS TO:
Dr. Goldberg
Anhthu Bui
Bekah Charney
Daisy Robinton
Brandon Le
Tomokazu Kawashima