Download - Autoclave (Primus)
STLCC-CPLS:Morrison 5/27/2015 Page 1
Prepared by: Bob Morrison
STLCC, Instrumentation Specialist
Dec 08, Last revision May 2015 (microburner)
Primus Model A Sterilizer
W RO water feed
@BRDG
Glasswasher @ BRDG
Tuttnauer Model 5596 SP-1
@ FV
Bacticinerators and Bead
Autoclave & Sterilizers (Detect Instrument Picture to Jump to that section of SOP)
Micro-Burner, Butane
Glasswasher; @BRDG, VWR
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Feature stainless steel construction for long life and corrosion protection.
Multiple cycle selections and microprocessor control ensure that each load is processed
efficiently and with the minimum energy required to meet cleaning requirements.
Operation is simple and loading is easy and flexible. A delayed-start feature allows the
washers to operate automatically unattended during off-peak hours.
Uses a maximum of 15L (3.9gal.) of tap water. Low water requirement and precise
detergent and neutralizer dosing minimize chemical costs
Spindle-ready models use an existing deionized water supply and can accommodate
either one or two direct injection spindle racks to clean up to 44 flasks.
Volume: 170 L (6 cu. ft.)
Exterior Dimensions,
Undercounter Unit: 61W x 62D x 87–93H cm (24 x 241/2 x 341/4–361/2")
Freestanding/Mobile Unit: 61W x 62D x 97.5H cm (24 x 241/2 x 383/8")(minimum height)
Interior Dimensions: 55 x 48 x 64 cm (211/2 x 19 x 251/4")
Clearance,
Standard Upper Open Rack: 25.7 cm (101/8")
Standard Lower Open Rack: 30.5 cm (12")
Spindle Rack-Ready Upper Rack*: 19.7 cm (73/4")
Spindle Rack-Ready Lower Rack*: 26 cm (101/4")
Electrical: 115V, 60Hz, 15A
*Maximum height with spindle clip at lowest position on spindle tube.
Washer with DI Rinse and DI Pump, Spindle-Ready 97021-364
Link to Manf website, Optional equipment, details….
Glasswasher: Control Panel, Detergent, @BRDG
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START; press
Stop; Hold >3 sec
High Heat Rinse
Button and LED High Heat Wash
Button and LED
Delayed Start and
LED (above)
PROG: Press to
set cycles; Max to
Rinse only
LED Displays Time
remaining in cycle
and current Temp.
MASTER ON/OFF
(Open Door to access)
Detergent;
Load main
and prewash
cups if
desired
Glasswasher: Cycles/Phases, Settings
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Autoclave: Sterilizer, BRDG, Primus, Clinical Model A
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Link to Primus Clinical –
Small Size Spec Sheet .pdf
Loading cart/gurney
Install date @BRDG 1/14/10 B-E Hospital Equipment Co, Inc.
Clinical Model A, SN 17413, Primus Sterilizer Co.
www.primus-sterilizer.com
316L Stainless Steel Chamber with Standard PriMirror
Interior Surface , Dimensions: 20”x20”x38”
PSS500 Microcomputer Control
Standard Vacuum, Gravity, Liquid and Test Cycles
Single, Manual, Vertical Sliding Door
Electric Steam Boiler, 208V, #4 ACW, 4 wire, 3ph, 67amps
Cybertech Thermal Printer with Take Up Reel
Drain Water Conservation Quench Includes Air Compressor
Safety Gloves
Emergency
Power OFF
Service:
636-343-8928
Autoclave: BRDG, Primus, MTC Services Sep 2013
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Clinical Model A, SN 17413, Primus Sterilizer Co.
www.primus-sterilizer.com
Alternative Services
CDS or ACE Lab Systems
Autoclave: Primus, BRDG, Control Panel, Defining Cycles
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Press buttons 1-4 to
choose those cycles.
Press up-arrow and
then 1-4 buttons to
access cycles 5-8
Changing Cycle Parameters:
1. Press Cycle to be changed, then press
MENU key, LED will light.
2. To change CycleType, press Enter,
then up arrow to see all types, press
Enter again to select a type.
3. To change other parameters on this
Cycle, use up arrow to scroll to the
next cycle parameter to be modified.
4. To change that value, Press Enter key,
“asterisk” key then lights up.
5. Use up/down arrows to change the
value (must be within allowable range
see pg 14)
6. Press ENTER key when done, then
continue up arrow to next parameter
7. Press ADV key to exit and return to
the idle screen for normal operations.
ADV Key; resets
any operation
(Exit)
OFF Key, press
to stop
operations
Lights: Indicate
current Cycle
phase or activity
Link to Primus Operator Manual .pdf
Displays current
state or cycle
and parameters
Autoclave: Starting a Cycle, Primus, BRDG
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STARTING A CYCLE, be patient
1. Press the keypad for the desired cycle, the
LED for that pad will turn on. For cycles
above 4, press the up arrow and keypad
numbers 1-4 (cycles 5-8)
2. Close the Sterilizer Door. You should see
confirmation with the Door Closed LED.
3. Wait ..…..The “START” button be not be
operative until the jacket temperature is
within 6 F of the cycle operating
temperature.
4. To view the Jacket temperature, press the
Upper left hand heart on the Primus
symbol.
5. When the jacket temperature has been
reached, the READY and START LEDs will
be lit.
6. Press the START button and hold for a few
seconds to begin the cycle. The READY
and START LED indicators will go out,
other cycle indicator LEDs will follow the
Cycle as it runs.
Door button: may
be required to
release door after
a cycle
Autoclave: Primus, Typical Cycle Diagram, Liquids
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Autoclave: Primus, BRDG Modified Cycles
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0
Autoclave: Primus, Gravity Cycle Description
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Autoclave: Primus, Liquids Cycle Description
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Autoclave: Primus, Vacuum Cycle Description
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Autoclave: Primus, Cycle Sequences
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Autoclave: Primus, Parameter Range
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Autoclave: Safety Guidelines, Primus, BRDG
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1. Never overload the Sterilizer, sterilization may never be achieved.
2. LIQUID cycles are only for the sterilization of Liquids, DO NOT USE
ANY OTHER CYCLE for Liquids.
3. When processing LIQUIDS, use containers with vented closures..do not use
ordinary glass bottles or any containers not designed for this purpose.
4. NEVER sterilize sealed containers in a cycle other than a LIQUIDS
cycle, rapid pressure changes in other cycles can cause explosive results.
5. NEVER obstruct the drains in the bottom of the chamber, always load in a
manner where no objects will touch or move to chamber walls.
6. ALWAYS use “hot gloves” when loading or unloading the sterilizer,
before or after a cycle…. ALWAYS assume the door, walls, racks,
and/or gurney are HOT!
7. ALWAYS place “hot gloves” on the ends of a loading cart
to indicate to others that the cart and labware are HOT!
Autoclave: Primus, BRDG,
Maintenance, Blow-down, Weekly (per Wally 11/28/11)
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WEEKLY – Execute this BLOW OUT protocol to
maintain boiler when unit is at operating
condition
1. Turn OFF boiler (toggle switch on rear of boiler)
2. Turn OFF mail 208V power switch (left front wall)
3. Turn OFF autoclave (control panel)
4. Turn OFF Main water supply valve at rear wall
5. Unplug all three 110V power cords at rear
6. Remove Right side Autoclave Stainless Panel
7. Using a protective glove locate Blow-Out Valve
(Green) at lower rear
8. Open valve slowly about 30 degrees
counterclockwise
9. Observe water/steam exiting to drain on wall
behind Millipore…. Wait until steam subsides
10. Open valve another 30 degrees, wait, and repeat
again until valve is fully open (aligned with the
exit pipe)
11. Return Valve to closed (pointing up) position
12. Turn ON main water supply valve
13. Turn ON main 208V switch and boiler toggle
switch (left rear)
14. Re-insert 110V power plugs
15. Watch Glass tube on side of boiler to ensure it
has refilled to acceptable level
16. Turn Autoclave back on
17. If RED Button (low boiler water level) on front
panel is lighted (out), press to reset
18. Resume operations
Blowdown line to
floor drain on other
side of the wall by
Millipore, steam exits
there.
Autoclave: Boiler Blowdown &
Maintenance (from Boiler manual)
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Autoclave: Primus, Boiler Plumbing Diagram
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Drain/Pressure
and Blowdown
release valve
1/4” Water
Supply line
Water level glass
tube
On/Off Master
switch (access
from rear of unit
Autoclave: Primus, PRI-Pure RO System Diagram 1/2
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Autoclave: Primus, PRI-Pure RO System Diagram 2/2
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Autoclave: Primus, Pri-Pure RO System
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Autoclave: Sterilizer, BRDG, Schematic
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Link to Primus Clinical –
Small Size Spec Sheet
110v 110v 110v
208V
Power 3 outlets
110V, 15Amp
For Boiler
208V, 67Amp
See Spec shts
Drain and Overflow Line
Primus
Clinical
Model A
Autoclave
B-E. Installation
Jeremy Jamerson
314-680-4248
Link to Primus Dwg
Millipore DI
water system
(ref only, not
connected)
208V 3-Phase
Power Switch, On/Up position
Bldg Cold Water
Feed Line
Remove Panel to
access Blow-out
Valve (Weekly)
RO Filters and 100L tank
To Boiler
Water Line Filter
non-boiler use
Bldg Cold
Water Feed
to RO/Boiler
Compressed
Air (not
used)
Autoclave: Primus PRI-Pure RO System
(Futuramics Clean Water, www.omahawater.com),
to Boiler and Water Line Filter (added June 2012)
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Autoclave: Primus, Water Line-feed Filter (Grainger; Aqua-Pure)
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Grainger Item # 2P275
Price (ea.) $124.75
Brand AQUA-PURE Mfr.
Model # AP101T
Valve in Head, Single High,
Size 3/4 In FNPT,
Color Clear,
Length 14 1/4 In, Width 4 9/16 In, Height 14 1/4 In,
Max. Temp. 100 F,
125 Max. Pressure (PSI),
Dia. 4 9/16 In.,
Reduces Dirt And Rust,
Plastic Housing Material,
Cartridges Required 1
Includes 1P753 Cartridge
Water Filter Cartridge,
Material of Construction Melt Blown Polypropylene,
Rating 5 Microns,
Application Rust and Dirt,
Flow 8 GPM,
Height 9 3/4 Inches, Diameter 2 5/8 Inches
Grainger Item # 1P753
Price (pk.) $25.55
Package Qty. 2
Brand AQUA-PURE
Mfr. Model # AP110
Recommended by Jeremy of B&E
4/26/12 for non-boiler related water use
http://www.aquapurefilters.com/store/product/00026.200018/ap101t.html
HotLink to Aqua-Pure Manual .pdf
Autoclave: Water Line Feed,
AP101T, Filter Replacement,
every 6mo
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Autoclave: Primus, Print Interval Setting
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Autoclave: Primus, Loading Thermal Paper
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Paper Can be Ordered from Supply
Concepts: Item #: SPR-25347 $2.24
Sparco Co.
Thermal Paper Roll, 2-1/4"x85', 3/PK,
Or OfficeMax:
Item# 20045997 Manf# OM98105
Thermal 2 1/4" x 85' 12rolls/pk $19.99
First : Remove the
Take-up Reel and
SAVE the take-up reel
by pushing it out of
the spool.
Autoclave: Primus, Loading Printer Take-up Spool
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Recommended Method (BobM)
1. Place empty spool in the area of the
hubs
2. Use a screwdriver to open/expand
the width of the open slot to make it
easier to snap over the hub ends
3. When empty spool is in place, fold
paper into a “V”, insert into spool
slot
4. Press FEED to take up some paper
on spool.
Autoclave: Thermometer, Temp Max after cycle
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Fisher Sci: CAT # 15-114-10 Each for $66.95
For use in autoclaves without built-in temperature recording devices
Has constricted capillary to hold temperature after heat is reduced so you can monitor maximum
temperature reached during sterilization.
Thermometer, Autoclave; Fisherbrand; Length: 200mm; Total immersion; Sun division: 0.5°C; Range:
85° to 135°C; Contains mercury
Autoclave: Temperature Loggers (example)
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Omega.com
Autoclave Temperature Data Logger
OM-CP-HITEMP140 , Approx $499
Specifications
Temperature Sensor: 100Ω Platinum RTD
Temperature Range (Body): -40 to 140ºC (-40 to 284ºF)
Temperature Measurement Range (Probe): -200 to 260ºC (-328
to 500ºF)
Temperature Resolution: 0.01ºC (0.02ºF)
Calibrated Accuracy: ±0.1ºC (±0.18ºF) [-20 to 140ºC (-4 to
284ºF)]
Start Modes:
• Software programmable immediate start
• Delay start up to eighteen months in advance
Real Time Recording: May be used with PC to monitor and
record data in real time
Memory: 32,700 readings
Reading Rate: One second up to once every 24 hours
Battery Type: 3.6V high-temperature lithium battery (included)
Battery Life: 1 year typical [1 minute reading rate at 25ºC (77ºF)]
Calibration: Digital calibration through software
Calibration Date: Automatically recorded within device
Data Format: Date and time stamped ºC, ºF, K, ºR
Time Accuracy: ±1 minute/month at 20 to 30ºC (68 to 86ºF)
(RS-232 cable not in use)
Computer Interface: OM-CP-IFC400 USB docking station
required 125,000 baud
Software: XP SP3/Vista/Windows® 7 (32- and 64-bit)
Operating Environment: -40 to 140ºC (-40 to 284ºF), 0 to 100%
RH
Dimensions
Body: 67 H x 25 mm D (2.7 x 0.9")
Probe: 50 L x 3.8 to 3.2 mm transitional dia (2.0 x 0.188 to
0.125")
Weight: 120 g (4.2 oz)
Material: 316 stainless steel
OM-CP-HITEMP140 3 Weeks $499.00
Autoclave temperature data logger qty.
OM-CP-HITEMP140-CERT $559.00
Autoclave temperature data logger and NIST certificate
qty.
OM-CP-IFC400 $120.00
Docking station with USB cable, software and manual
qty.
OM-CP-BAT110 $50.00
Replacement 3.6 V high-temperature lithium battery
Autoclave FV ; General Information
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Description: Tuttnauer 5596 SP -1 Sterilizer Dimensions: 43” W x 56” D x 75” H (External Stainless walls with enclosed boiler and pump. ) Requires: DI and standard cold water input and 1.5” exhaust/vapor line. Clearances: 500mm on sides and rear. Front should have clear access for trays on carts. • Chamber size is 15" dia x 29.5" deep.
Chamber Dimensions WxDxH mm (inc.): 508 x 508 x 970
Chamber Volume (Liters): 250
Total Weight with all components 1000 KG
• High speed, high vacuum, high capacity, hospital grade sterilizer with FDA cleared flash cycle • A built in vacuum pump and steam generator allows for increased load size and dramatically reduces total cycle time. • The closed door drying system provides a faster and more thorough drying cycle. Seven automatic cycles are available. • Five sterilization cycles and two test cycles. • Designed for sterilization of medical and surgical materials such as packed and wrapped solids and instruments.
Link to B-E Hospital Equip Co, Inc .
STL sales/service
Wally Rodemacher tech Spec.
314.241.0855
• Chamber Size: 20x20x38 with Manual Hinged Door
• Chamber Volume: 250 Liters
• External Dimensions: 30”W x 56”D x 75”H
• Carbon Steel Electric Steam Generator (Integral)
• Free-Standing with Stainless Steel Cabinet Enclosure
• Standard Cycles: (4)Gravity, (4)Pre-Vac, (4)Liquid and (2)Test Cycles
• Utilities required (by others): Water (approx. 50Mohm), 3-phase electric, Compressed air, Floor Drain
• Designed for sterilization of porous and non-porous material, liquids, glassware etc.
• Warranty: 1 year parts and labor, 15 years on the pressure vessel
• Accessories: Rack with two shelves
Options not included:
• Integral air compressor (if house air is not available)
• Auto- Vertical Sliding Door (Model 5596 ECP-V)
Autoclave: FV-Tuttnauer Model 5596
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Link to General Notes for
Architects/Contractors .pdf
Link to Installation and Clearance
Drawing .pdf
Autoclave: General Information from: David Morganstern Sales Manager tuttnauer USA
25 Power Drive Hauppauge, NY 11788Ph: 800-624-5836 x112 Fax: 631-737-1034 E-mail:
Autclave FV; Inside Panel Views; Tuttnauer 5596
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Air Compressor and tank; out for
service goes into lower bay
Boiler for Steam/Pressure
RODI Water storage from
Millipore system
Autoclave : Tuttnauer Basic Operating Instructions
1. Open the main steam inlet valve and watch the jacket pressure gauge on the front
panel. When the jacket pressure reaches 2.3 bars, the sterilization cycle can be
started. (condensate may exit through the jacket steam trap during this period)
2. Open the door by turning the control wheel handle counter clockwise to release the
locking arms.
3. Load the material to be sterilized per the guidelines in this protocol
4. Close the door by pushing towards the frame and turning the wheel handle
clockwise until the “Door Closed” lamp is lit.
5. On the control panel, chose the sterilization program according to the materials
loaded. The light for the program should be on with parameters displayed.
6. Press the START key to begin the cycle. Lights on the bottom indicate the stage of
the cycles in progress. – Air is purged from the chamber at the beginning of the sterilization cycle.
– Steam under pressure is introduced into the chamber and the temperature rises to the desired level.
– The autoclave is maintained under pressure at a high temperature for a predetermined time.
– Steam is then exhausted from the chamber at the end of the cycle.
7. A buzzer will sound and the START light will turn off at the end of the cycle. The
printer will print a summary of the cycle.
8. Open the door by turning the wheel handle counter clockwise.
9. Remove sterilized materials carefully using orange or heat resistant gloves
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Autoclave : Tuttnauer Safety Features and Audible Alarms 1. Steel bar Door Locks
2. Safety Vent/Vent Valve
• Safety valve – opens as back-up protection to reduce chamber pressure in the
event of exceeding 262 kPa
• Vent Valve – needed if chamber pressure should exceed 241kPa, the vent
valve will open and the P-2 alarm will display.
3. Always use “HOT GLOVES” (orange) when removing items
from the Autoclave
1. Overheat Protection – chamber temperature is set so as not to exceed 149 C and has
additional overheat protection if temperature reaches 180 C.
2. Electrical power interruption – in case of an electrical power failure during the
sterilization cycle, pressure in the chamber is automatically vented to the atmosphere.
3. Audible Signals
– Each time a switch is depressed one beep occurs (except when depressing the four arrows).
– Five beeps indicates sterilization cycle is complete.
– Five beeps indicates drying cycle is complete.
– Six beeps operational error or alarm.
– Continuous beeps – door is open during fill cycle.
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Autoclave : Tuttenaeur
Control and Monitoring Keys
• Unwrapped Instruments (without drying stage)
• Wrapped Instrumentss (with drying stage)
• Liquids; pressing one of the program keys
determines the stages to be run
• Vacuum test/Bowie Dick test; press once for
Vacuum, twice for Bowie test
• TEMP; shows sterilization temperature. Press
UP/DN keys to permanently change this value
from the nominal setting.
• STE Time; shows sterilization time. Use
UP/DN to permanently alter this setting.
• DRY Time; shows drying time. Alter with
UP/DN keys.
• Clock ; Press to change settings with UP/DN
keys.
• START; begins selected sterilization program.
Will not start if door in not secured.
• STOP; Only key that can be used to stop
operations once a program cycle has begun.
PROGRAM CYCLE or STATUS
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Autoclave: Tuttnauer Sterilizing Media in Flask; Setup
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1. Make sure pressure Jacket
pressure is at or above 18 psi
2: Select Liquids on control
panel
(prepared from: E. Lyons 10/22/08)
Autoclave: Tuttnauer Sterilizing Media in Flask; Run Settings, Door
operations
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3. Check to make sure that the
autoclave is set to run for 20 minutes
at 250F. (The photo shows that this is
program 12, and the temperature and
time are set correctly.)
4. Open the autoclave door by turning
counter clockwise. Place the flasks in
the autoclave with some room
between them. Close the door tightly
with clockwise motion.
Close Door:
Clockwise
CW
Open Door:
Counter
Clockwise
CCW
(prepared from: E. Lyons 10/22/08)
Autoclave: Tuttnauer Sterilizing Media in Flask ; Start and Run
Verify
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5. Press the START button on
the control panel.
6. Check to make sure that the
autoclave has functioned properly
during the heating cycle by checking
the recording tape for errors. (This
tape can be copied for inclusion in
your lab notebook.)
(prepared from: E. Lyons 10/22/08)
DATE 4/13/2009 TIME 4:36:07
PROG Liqud1
TEMP 250F TIME 20 min DRY 00 min EXH MODE 4 END TEMP 203 MN TEMP PRESS V01 231F 15P V02 201F 15I S04 250 17P S05 252 17P S06 252 17P S07 252 17P S08 252 17P S09 252 17P …. … … S23 252 17P S24 251 16P E25 247 13P E28 229 04P E31 218 00P E34 215 00P E37 210 00P E40 210 01I E43 210 01I E46 210 01I E49 204 01I E49 204 01I O.K. Operator Load NO 5 Autoclave No. 12
Autoclave: Tuttnauer Run Print Tape
Examples
PROGS: Liqud1, UnWrp1, UNWrp3
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DATE 4/13/2009 TIME 4:36:07
PROG UnWrp1 TEMP 250F TIME 20 min DRY 10Min EXH MODE 1 MN TEMP PRESS V01 231F 15P V03 191 15I S04 250 17P S05 252 17P S06 252 17P S07 253 17P S08 252 16P …. … … S18 255 18P E20 237 03P D20 183 23I D22 197 22I D28 168 23I D30 162 22I O.K. Operator Load NO 7 Autoclave No. 12
DATE 4/13/2009 TIME 4:36:07
PROG UnWrp3 TEMP 270F TIME 05 Min DRY 00Min EXH MODE 1 MN TEMP PRESS F08 079F 01P F09 079F 01P Operator Load NO. 2 Autoclave No. 12
Autoclave: Sterilizing Media in Flask ; Removing and Cooling
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7a. When the autoclave is finished and the inside
pressure has dissipated, remove your flask of media
using orange hot gloves.
7b. Swirl the media gently because the agar may
settle to the bottom of the flask during autoclaving.
8a. Place the media into a water bath set at 55-60C,
which is the temperature it should cool to for pouring
the plates. (Note: If the solution is not placed in a water
bath, cool until you can just hold your bare hands on
the bottom of the flask.
8b. If it begins to solidify, it will have to be re-melted by
autoclaving again for about 5 minutes.
(prepared from: E. Lyons 10/22/08)
Autoclave: Sterilization Process and Verification
• Sterilization – the highest level of disinfection; the killing of all living
organisms on a surface
• Testing whether sterilization has occurred during autoclaving is done
using spores (dormant microorganisms)
– Spore strips are dried pieces of paper to which large number of
pathogenic , heat-resistant Bacillus thermopholus spores are
adhered.
– After autoclaving the strips are transferred to growth medium, if
successful autoclaving, then the spores will not grow.
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Autoclaves: Cycles, Gravity vs. Vacuum
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Gravity Cycles: Displacement Autoclaves – The Old Workhorse
Traditional models rely upon gravity to displace the air from the sterilizing chamber. Steam is much lighter than air and, as it is
injected into the pressure chamber, it forces air downwards, trapping it in a sink. A system of valves and taps bleed out this excess
air, allowing the steam to sterilize the contents of the chamber.
These autoclaves are the cheapest option and they are very easy to use. As a result, they are common in kitchens and manicurists,
where they are more than adequate. However, for sterilizing porous or wrapped materials, displacement autoclaves are not efficient
enough. Hot air may be trapped inside, allowing pockets of microbes and other pathogens to evade the sterilization cycle.
Vacuum Autoclaves – The Safer Solution to Sterilization
For complex sterilization, or in areas where complete sterility is necessary, a vacuum autoclave is the superior choice. Vacuum
autoclaves evacuate the air, with a vacuum, and steam is then injected into the chamber. This allows the steam to penetrate porous
materials, wiping out any pockets of pathogens in hard-to-reach areas.
After this steam sterilization stage, the pump again evacuates the chamber, drying the autoclave contents thoroughly. This added
stage prevents germs and prions being retransmitted by trapped moisture. Vacuum autoclaves are the preferred option in medical
institutions, as a more effective alternative to the simpler displacement autoclaves.
Many hospitals use disposable medical equipment, to prevent the transmission of CJD variants, but the contaminated waste is still
autoclaved before landfill. Autoclaving is a more environmentally friendly method than traditional incineration, which ejects toxic
gases into the atmosphere.
Autoclave; Tuttnauer 5596 SP Series
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Autoclave : Tuttnauer Utilities Consumption
• 16”x16”x28”
20”x20”x38”
• H44 • D
• W
• H
• D
• W
• H
• D
H55 Series
Autoclave/Sterilizer: BRDG Proposal, STERIS/AMSCO-Lab 250
STLCC-CPLS:Morrison 5/27/2015 Page 47
The Amsco Lab 250 sterilizer was designed with the needs and
the budget of the laboratory research market in mind. The lab
series sterilizer provides the same time-proven, safe, reliable
chamber and stand assembly as the Century® sterilizer that has
been offered to the healthcare and life sciences markets since
1992.
The Amsco Lab 250 features an Allen-Bradley MicroLogix"
control and a full-color operator interface. The unit has a
chamber size of 20 x 20 x 38 inches (508 x 508 x 965 mm) and
is equipped with gravity and liquid type cycles as standard.
Optional prevacuum and isothermal cycles are also available if
required. A manually operated vertical sliding door is combined
with the non-lubricated door seal for reliability and ease of use.
The drain line piping package meets or exceeds the maximum
water temperature requirements of the industry - 60ºC (140ºF).
Link to AMSCO Lab 250 Spec Sheets…. pdf
Link to AMSCO Lab 250 Brochure…. pdf
Link to AMSCO Lab 250 Tech Sheets…. pdf
Autoclave/Sterilizer: BRDG Proposal,
AMSCO Lab 250 Spec Sheet
STLCC-CPLS:Morrison 5/27/2015 Page 48
Autoclave/Sterilizer: BRDG Proposal,
AMSCO Lab 250 Spec Sheet
STLCC-CPLS:Morrison 5/27/2015 Page 49
Sterilizer: Bacti-Cinerator
STLCC-CPLS:Morrison 5/27/2015 Page 50
• Sterilizes loops and needles safely and conveniently.
• Prevents infectious spatter and/or cross contamination.
• Safeguard laboratory personnel -no open flame -no
hazardous gas.
• Ideal for use in anaerobic chambers.
• Core Tube does not contain asbestos
On/off toggle switch
Heat element “on” indicator
Oxford® Bacti-Cinerator® IV (Cole-Palmer)
EW-01850-26
Replacement Heating Element for 01850-22 (120V)
Sterilizer; Bacti Cinerator- Features, Safety
• Allow 6-10 minutes for the Bacti-Cinerator to reach an
optimum sterilizing temperature of 815°C (1500°F) throughout the interior of the core.
• 5-7 seconds of exposure sterilizes loops, needles and culture tubes. Unit is not intended
for use with scalpels, forceps or sharp instruments.
• Use only insulated loop holders when inserting loops into the heater element.
• Suitable for anaerobic procedures in chambers and hoods.
• Yellow Light indicates that the heating element is ON. Note, the Core element may
remain very hot for 30 minutes after it is turned off. Allow 1 hour of cooling before
cleaning or storage.
• Avoid any contact with the heat shield or end caps while the Sterilizer is on.
• Do not immerse the sterilizer in water or other cleaning solutions.
• Periodically inspect the element for cracks (yellow-orange fissures) and wear.
STLCC-CPLS:Morrison 5/27/2015 Page 51
Core will glow red-hot
when approx 815C temp has
been reached
Extremely Hot surface. Do not
touch or allow other items to
come in contact
Do not wrap power cord around
Heat element for storage.
Sterilizer: Bead
STLCC-CPLS:Morrison 5/27/2015 Page 52
Effectively sterilize small, solid metal and glass implements in
seconds
• Heated glass beads destroy all microbes and spores
• Low power consumption allows for all day operation
• Safe to use—no gases, fumes, open flames or harsh liquids
STLCC-CPLS:Morrison 5/27/2015 Page 53
Bead Sterilizer: Setup and Operating Protocol
1. Sterilizer Usage:
• Remove the metal cover cap before turning on the unit
• Allow 15 minutes to reach 240C before use
• Unit can be left on for further use
• Turn off at the end of the day
• Replace metal cap when cooled to prevent contamination
2. Caution: Do not touch beads or sides of container when in use.
3. Small Dish:
• Use for small metal objects
• Cover dish with metal cap for 3 minutes maximum
• Remove dish and cap when finished
4. Glass beads are heated to 250°C to effectively destroy all microbes and spores.
Suitable for sterilizing scissors, forceps, scalpels, inoculating loops and needles.
5. Power consumption is low allowing for all day operation.
6. Dial thermometer on front of sterilizer ensures proper heating and back-up safety
thermostat prevents over-heating.
Sterilizer/Heat Source- Micro Burner Butane
STLCC-CPLS:Morrison 5/27/2015 Page 54
This small butane burner uses clean burning butane and features a
trigger ignition, eliminating the need for wicks, matches or a central
gas line. Avg use rate 2oz/hr, refill about every 45min
Sterilizer/Heat Source- Micro Burner Butane
STLCC-CPLS:Morrison 5/27/2015 Page 55