Download - Av-mb-sop-010 (Bis) Sop for Isolation and Identification of Coliforms by Pour Plate Method
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7/27/2019 Av-mb-sop-010 (Bis) Sop for Isolation and Identification of Coliforms by Pour Plate Method
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Department: Quality Assurance DOC. No. AV/MB/SOP/010
Page No. : Page 1 of 4
Effective Date: 01.06.06 Revision No. : 00
Subect SOP for isolation ! i"entification of Coli forms
RE#$S$ONS
Revision % Revise" Pages Description Date
00 Original issue &'.&(.&(
Prepare" )y Approve" )y Aut*ori+e" by
Microbiologist Management Representative actor! Manager
"#$ASS%%&'( %nternal )se Onl! "
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7/27/2019 Av-mb-sop-010 (Bis) Sop for Isolation and Identification of Coliforms by Pour Plate Method
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Department: Quality Assurance DOC. No. AV/MB/SOP/010
Page No. : Page * of 4
Effective Date: 01.06.06 Revision No. : 00
Subect SOP for isolation ! i"entification of Coli forms
'.& Purpose
+o ,efine proce,-re for #oli forms b! po-r plate meto,.
,.& Scope
+is proce,-re is applicable to ,etermine #oli forms in a given sample.
-.& Responsibilities
Microbiologist
AM-alit!
.& Definition :
SOP( Stan,ar, operating proce,-re.
/.& Proce"ure
/.' +est portion initial s-spension an, ,il-tions(2%SO 6335
/., $noculation an" incubation:0
/.,.' +ae t7o sterile Petri ,ises. )sing a sterile pipettetransfer to eac ,is 1 ml of test sample if te pro,-ct is
li8-i, or 1 ml of te initial s-spension in case of oter
pro,-cts.
/.,., +ae t7o oter sterile Petri ,ises. )sing a fres sterile
pipette transfer to eac ,is 1 ml of te first ,ecimal
,il-tion 21015 of te test sample if te pro,-ct is li8-i,
or 1 ml of te first ,ecimal ,il-tion 210 *5 of te initial
s-spension in case of oter pro,-cts.
/.,.- Repeat te proce,-re 7it te f-rter ,il-tions.
/.,. Po-r abo-t 19 ml of te VRB$ me,i-m at 49:# ; 0.9:#
into eac Petri ,is./.,./ +e time elapsing bet7een te en, of te preparation of
te initial s-spension an, te moment 7en te me,i-m
is po-re, into te ,ises sall not e
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7/27/2019 Av-mb-sop-010 (Bis) Sop for Isolation and Identification of Coliforms by Pour Plate Method
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Department: Quality Assurance DOC. No. AV/MB/SOP/010
Page No. : Page > of 4
Effective Date: 01.06.06 Revision No. : 00
Subect SOP for isolation ! i"entification of Coli forms
/.,.3 %nvert te prepare, ,ises an, inc-bate tem in inc-bator
set at >0:#>9:# or >:# for *4 o-rs ; * o-rs.
/.- Counting of t*e colonies
After te specifie, perio, of inc-bation co-nt -sing colon!
co-nting e8-ipment te colonies in eac ,is containing not
more tan 190 colonies.
E4pression of results:0
/. 5et*o" of calculation
/..' ?eneral case @ 'ises containing bet7een 19190
colonies
/..'.' Retain ,ises containing not more tan 190
colonies at t7o consec-tive ,il-tions. %t is
necessar! tat one of tese ,ises contains at least
19 caracteristic colonies.
/..'., #alc-late te n-mber N of #oli forms per
milliliter or per gram of pro,-ct ,epen,ing onte case -sing te follo7ing e8-ation(
6 C N 7 00000000000000000000 8n'9 &.' n, d
ere
6 C is te s-m of colonies co-nte, on all te ,ises retaine,
n' is te n-mber of ,ises retaine, in te first ,il-tions n, is te n-mber of ,ises retaine, in te secon, ,il-tions
d is te ,il-tion factor correspon,ing to te first ,il-tion.
Ro-n, te res-lt calc-late, to t7o significant fig-res.
/..'.- +ae as res-lt te n-mber of microorganisms per
milliliter or per gram of pro,-ct e
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7/27/2019 Av-mb-sop-010 (Bis) Sop for Isolation and Identification of Coliforms by Pour Plate Method
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Department: Quality Assurance DOC. No. AV/MB/SOP/010
Page No. : Page 4 of 4
Effective Date: 01.06.06 Revision No. : 00
Subect SOP for isolation ! i"entification of Coli forms
/..,.' %f te t7o ,ises correspon,ing to te test sample2li8-i, pro,-cts5 or te initial s-spension 2oter
pro,-cts5 contain less tan 19 colonies calc-late
te aritmetic mean mof te colonies co-nte, on
bot ,ises.
/..,., Report te res-lts as follo7s(
estimate, n-mber of #oli forms per milliliter(
NE 7 m8li;ui" pro"ucts5
estimate, n-mber of #oli forms per gram(
NE 7 m4 d0'2oter pro,-cts5 7ere , is te
,il-tion factor of te initial
s-spension./..- No c*aracteristic colonies
/..-.' %f te t7o ,ises correspon,ing to te test sample
2li8-i, pro,-cts5 or te initial s-spension2oter
pro,-cts5 contain no colonies report te res-lts as
follo7s(
less tan 1 #oliform per milliliter2li8-i,
pro,-cts5
less tan 1 < ,1 #oliform per gram2oter
pro,-cts5 7ere , is te ,il-tion factor of te
initial s-spension.
(.& Documentation :
Microbiological +est Report
2.& References:%S 9401 2PAR+ 15(*00*
Prepare" )y Approve" )y Aut*ori+e" by
Microbiologist Management Representative actor! Manager
"#$ASS%%&'( %nternal )se Onl! "