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Blocking C-Raf alleviated high-dose small-volume radiation-induced epithelial mesenchymal transition in mice lung
Zhen-Yu Honga,b#, Sanke Lic#, Xiaomei Liua, Xiao-Min Lengd, Zhanhui Miaoa, Xiaohong Kanga, Hongrui Niua, Ming-Qing Gaoe*, Ping Lua*
a Department of Medical Oncology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China. b Department of Radiation
Oncology, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, South Korea. c Department of Hematology, the 3rd Peo
ple’s Hospital of Zhengzhou, China. d Henan Key Laboratory of Neural Regeneration, Department of Neurology, The First Affiliated Hospital of Xinxia
ng Medical University, Weihui, Henan, China. e School of Medicine, Northwest University, Shaanxi, China
# Zhen-Yu Hong and San-Ke Li contributed equally to the manuscript and share first authorship
*Reprint requests to:
1. Ping Lu, MD, PhD.
Professor and Head
Department of Oncology,
The First Affiliated Hospital of Xinxiang Medical University,
88 Jiangkang Road, Weihui, Henan, P.R. China
Zip 453100, Office) +86-373-440-2543
Mobile) +86-135-9872-2864 E-mail) [email protected]
&
2. Ming-Qing Gao, PhD
Professor
School of Medicine, Northwest University.
Taibai North Road 229, Xi’an 710069, Shaanxi, P.R. China
E-mail) [email protected]
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Supplementary Figure S1. Full unedited gel for Figure 2 A
Control 70Gy Xray
2W 4W 2W 4W
p-C-Raf C-Raf
Control 70Gy Xray
2W 4W 2W 4W
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Supplementary Figure S2. Full unedited gel for E-cadherin in Figure 3 B, and repeated experiment with different exposure time
24h 48h 72h
8 Gy
control
repeated experiment with different exposure time
Blots in main Figure 3B Original Blots of E-cadherin
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Supplementary Figure S3. Full unedited gel for α-SMA in Figure 3 B, and repeated experiment with different exposure time
24h 48h 72h control
8 Gy
Original Blots of α-SMA
Blots in main Figure 3B
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24h 48h 72h control
8 Gy
24h 48h 72h control
8 Gy
Supplementary Figure S4. Full unedited gel for GAPDH in Figure 3 B, and repeated experiment with different exposure time
Original Blots of GAPDH
repeated experiments with different exposure time
Blots in main Figure 3B
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Supplementary Figure S5. Full unedited gel for p-C-Raf in Figure 4 A
p-C-Raf
Original Blots of p-C-Raf
Blots in main Figure 4A
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Supplementary Figure S6. Full unedited gel C-raf In for Figure 4 A
C-raf
Original Blots of C-Raf
Blots in main Figure 4A
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Supplementary Figure S7. Full unedited gel for GAPDH in Figure 4 A
Original Blots of GAPDH
Blots in main Figure 4A
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Supplementary Figure S8. Full unedited gel for p-C-Raf in Figure 4 B
p-C-Raf
Original Blots of p-C-Raf
Blots in main Figure 4B
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Supplementary Figure S9. Full unedited gel for GAPDH in Figure 4 B
GAPDH
Original Blots of GAPDH
Blots in main Figure 4B
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Supplementary Figure S10. Full unedited gel for E-cadherin in Figure 4 C
Control 8 Gy GW5074 8 Gy +
GW5074
Original Blots of E-cadherin
Blots in main Figure 4C
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Supplementary Figure S11. Full unedited gel for α-SMA in Figure 4 C
Control 8 Gy GW5074 8 Gy +
GW5074
Original Blots of α-SMA
Blots in main Figure 4C
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Supplementary Figure S12. Full unedited gel for GAPDH in Figure 4 C
Control 8 Gy GW5074 8 Gy +
GW5074
Original Blots of GAPDH
Blots in main Figure 4C
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Supplementary Figure S13. Full unedited gel for Twist1 in Figure 5 A, and repeated experiment with different exposure time
repeated experiments with
different exposure time
Blots in main Figure 5A
Original Blots of Twist1
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Supplementary Figure S14. Full unedited gel for GAPDH in Figure 5 A, and repeated experiment with different exposure time
Blots in main Figure 5A
Original Blots of GAPDH
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Supplementary Figure S15. Full unedited gel for α-SMA in Figure 5 B, and repeated experiment with different exposure time
8Gy - + + +
GW5074 - - + -
sh-Twist1 - - - +
Original Blots of α-SMA
repeated experiments with different exposure time
Blots in main Figure 5B
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Supplementary Figure S16. Full unedited gel for GAPDH in Figure 5 B, and repeated experiment with different exposure time
8Gy - + + +
GW5074 - - + -
sh-Twist1 - - - +
Blots in main Figure 5B Original Blots of GAPDH
repeated experiments with different exposure time
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8Gy - + + +
GW5074 - - + -
sh-Twist1 - - - +
Supplementary Figure S17. Full unedited gel for p-C-Raf in Figure 5 B, and repeated experiment with different exposure time
Original Blots of p-C-Raf Blots in main Figure 5B
repeated experiments with different exposure time
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8Gy - + + +
GW5074 - - + -
sh-Twist1 - - - +
Supplementary Figure S18. Full unedited gel for E-cadherin in Figure 5 B, and repeated experiment with different exposure time
repeated experiments with different exposure time
Original Blots of E-cadherin Blots in main Figure 5B
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8Gy - + + +
GW5074 - - + -
sh-Twist1 - - - +
Supplementary Figure S19. Full unedited gel for Twisr1 in Figure 5 B, and repeated experiment with different exposure time
Original Blots of Twist1
Blots in main Figure 5B
repeated experiments with different exposure time