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CHAPTER 8 Part 2
Cytoplasmic Membrane Systems:
Structure, Function, and
Membrane Trafficking
Copyright © 2008 by John Wiley & Sons, Inc.
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ER to the Golgi Complex: The first step in vesicular transport ERGIC Region
Visualizing membrane traffic with a fluorescent tag
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Vesicular Transport from the ER to the Golgi
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The Golgi Complex
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Glycosylation in the Golgi Complex
No need to memorize the steps; only the concept is important
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Dynamics of Transport through the Golgi Complex
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COP1 and COPII Coated Vesicles
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Vesicular Transport between membranousCompartments of the biosynthetic/secretorypathway
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Roles of COPII proteins in assembling protein coat and capturing cargo
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Accumulation of COPI- coated Vesicles
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Retrieval of Resident ER Proteins !
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Protein Transport from one Golgi cisternae to another can be assayed in a cell-free system (example: cultured fibroblasts); Fig a & b
Fig. a
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VSV G protein in vitro contains the additional N-acetylglucosamine!!!This modification is carried out by transferase enzyme that is moved by
retrograde transport vesicles from the wild-type medial-Golgi cisternae to the mutant cis-Golgi cisternae in the reaction mixture (Science 276:1212, 1997).
Fig. b
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VERY IMPORTANT DIAGRAM !Read Legend in Text-book; Fig 8.29
Targeting Lysosomal Enzymes To Lysosomes
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Formation and Fusion of a Transport Vesicle
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Formation of Calthrin coated Vesicles at the TGN
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Model for Targeting of Transport Vesicles to Target Membranes
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Model for interaction between v- and t-SNAREs leading to membrane fusion and exocytosis
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LYSOSOMES
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Lysosomes
Liver Cells Neuron of a Human withLysosomal Storage DisorderAccumulation of unidgested glycolipids
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