Download - Cystic Fibrosis From Gene to Protein
![Page 1: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/1.jpg)
Cystic FibrosisFrom Gene to
ProteinClass instructions
![Page 2: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/2.jpg)
In this lesson
• Look at the normal and mutated CFTR gene
• Make messenger RNA (mRNA) from DNA- transcription
• Make protein from mRNA- translation
![Page 3: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/3.jpg)
The devil is in the detail!
• The 5’ prime and 3’ prime ends of the bases must be round the right way!
IMPORTANT: Do not take
bases apart!!!
![Page 4: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/4.jpg)
Correct base pairing is critical!
• Green (Guanine) pairs with yellow (Cytosine)
• Blue (Adenine) pairs with orange (Thymine)
![Page 5: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/5.jpg)
Lesson – part 1Normal CFTR Gene ΔF508 CF Mutation
Sense
Antisense
Sense
Antisense
![Page 6: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/6.jpg)
Transcription – Step 1
![Page 7: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/7.jpg)
Base pairing in mRNA- use brown sugars
• Green (Guanine) pairs with yellow (Cytosine)
• Blue (Adenine) pairs with light blue (Uracil)
![Page 8: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/8.jpg)
Transcription – Step 2• To make an mRNA, you must use the
antisense (red sugars) strand as as the template as base pairing is complimentary.
![Page 9: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/9.jpg)
Resulting mRNA
Normal CFTR mRNA ΔF508 CFTR mRNA
![Page 10: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/10.jpg)
Translation
• Turns mRNA into protein• Use transfer RNA (tRNA) which are
attached to amino acids as intermediate
• Amino acids have a direction too!
N-terminal C-terminal
![Page 11: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/11.jpg)
Transfer RNA
UAAIsoleucine
CAAValine
CCAGlycine
AAAPhenylalanine
UAAIsoleucine
tRNA – holds an anticodon at one end, and an amino acid at the other
![Page 12: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/12.jpg)
Using mRNA in 5’-3’ orientation, translate into
protein.
![Page 13: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/13.jpg)
Separate tRNA & protein from mRNA
![Page 14: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/14.jpg)
Resulting protein
Isoleucine – Phenylalanine - Glycine
Isoleucine – Glycine - Valine
Normal CFTR protein
ΔF508 CFTR protein
![Page 15: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/15.jpg)
Polymerase Chain Reaction
• Using the original chromosomes, separate strands
• Find where primers stick to each strand
![Page 16: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/16.jpg)
Genetic Test • Using the original chromosomes, see what a PCR
product will look like.• Remember: Each individual will have 2 chromosomes
- one inherited from mother and one from father.
![Page 17: Cystic Fibrosis From Gene to Protein](https://reader033.vdocument.in/reader033/viewer/2022052701/56813bc6550346895da4f1d9/html5/thumbnails/17.jpg)
DNA gel genetic test result