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DEPARTMENT OF PATHOLOGY AND LABORATORY MEDICINE
2010-2011 ANNUAL REPORT
TABLE OF CONTENTS
Faculty Roster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Research and Scholarly Accomplishments . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Teaching . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Medical Teaching . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Dental Teaching . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Molecular and Cellular Pathology Graduate Program . . . . . . . . . . . . . . . . . . . . 43
Residency Training Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Subspecialty Fellowship Training Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Clinical Chemistry Fellowship . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Clinical Microbiology Fellowship . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Clinical Molecular Genetics Fellowship . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Clinical Molecular Pathology Fellowship. . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Cytogenetics Fellowship. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Cytopathology Fellowship. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Forensic Pathology Fellowship. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Hematopathology Fellowship. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Nephropathology Fellowship. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Neuropathology Fellowship. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Surgical Pathology Fellowship. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Transfusion Medicine Fellowship. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Grand Rounds Seminars . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Environmental Pathology Training Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Clinical Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Background. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
McLendon Clinical Laboratories
Herbert Whinna, M.D. Ph.D., Director
Surgical Pathology (Histology/Special Procedures) . . . . . . . . . . . . . . . . . . . . 57
William K. Funkhouser, M.D., Ph.D., Director
Cytopathology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Susan J. Maygarden, M.D., Director
Autopsy Pathology. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Leigh B. Thorne, M.D., Director
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Molecular Pathology. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Margaret L. Gulley, M.D., Director
Transfusion Medicine, Apheresis, Transplant Services. . . . . . . . . . . . . . . . . . 60
Herbert C. Whinna, M.D., Ph.D., Director
Clinical Microbiology, Immunology. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Peter H. Gilligan, Ph.D., Director
Phlebotomy. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Peter H. Gilligan, Ph.D., Director
Core Laboratory (Chem./UA/Coag./Hem/Tox/Endo) . . . . . . . . . . . . . . . . . . . 64
Catherine Hammett-Stabler, Ph.D., Director
Hematopathology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Cherie H. Dunphy, M.D., Director
Special Coagulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Herbert C. Whinna, M.D., Ph.D., Director
Cytogenetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Kathleen W. Rao, Ph.D., Director
Laboratory Information Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Herbert C. Whinna, M.D., Ph.D., Director
Nephropathology Laboratory. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Volker R. Nickeleit, M.D., Director
Quality Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Herbert C. Whinna, M.D., Ph.D., Director
Neuropathology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Thomas Bouldin, M.D., Director
Outreach Laboratory Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Herbert C. Whinna, M.D., Ph.D., Director
Transplant Laboratories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
John L. Schmitz, Ph.D., Director
Muscle Pathology Laboratory. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Leigh B. Thorne, M.D., Director
Human Progenitor Cell Laboratory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Yara A. Park, M.D., Director
Core and Service Laboratories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Microscopy Services Laboratory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Laser Capture Microdissection Core Facility. . . . . . . . . . . . . . . . . . . . . . . . . 71
Translational Pathology Laboratory (TPL). . …………. . . . . . . . . . . . . . . . . 71
Animal Clinical Laboratory Facility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Gene Expression Facility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
DNA Synthesizing Facility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
ADME Mass Spectrometry Facility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Faculty and Senior Staff Changes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Special Honors and Awards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
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Elected Leadership Positions . . . . . . . . . . . .. . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . .. 77
Leadership Positions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Member of Board of Directors of National/International Accreditation Agency . . . . 82
Member of FDA, CDC, or Comparable Committee. . . . . . . . . . . . . . . . . . . . . . . . . . 83
Member of NIH or Comparable Study Sections . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Service as Editor or on Editorial Boards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Invited Lectures at State, National or International Meetings . . . . . . . . . . . . . . . . . . 90
Director of Continuing Education Courses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Service on UNC and UNCH Committees . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Departmental Faculty Handbook . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Departmental Web Site . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Publications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
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DEPARTMENT OF PATHOLOGY AND LABORATORY MEDICINE
FACULTY AND TRAINEE ROSTER
2010-2011
Chair
J. Charles Jennette, M.D., Brinkhous Distinguished Professor and Chair
Vice Chairs
Thomas W. Bouldin, M.D., Professor and Vice Chair for Faculty and Trainee Development
William K. Funkhouser, M.D., Ph.D., Professor and Director of Anatomic Pathology and
Associate Director of McLendon Clinical Laboratories
Herbert C. Whinna, M.D., Ph.D., Associate Professor and Director of McLendon Clinical
Laboratories and Vice Chair for Clinical Services
David G. Kaufman, M.D., Ph.D., Professor and Vice Chair for Research Development
Associate Chair for Administration
Nancy H. Nye
Distinguished Professors
Joe W. Grisham, M.D. (Kenan Distinguished Professor, Emeritus)
Nobuyo N. Maeda, Ph.D. (Robert H. Wagner Distinguished Professor)
Marjorie S. Read, Ph.D. (Fred C. & Lelia Owen Prof., Emeritus)
Oliver Smithies, D.Phil. (Kay M. & Van L. Weatherspoon Eminent Distinguished Professor)
Richard R. Tidwell, Ph.D. (Kenan Distinguished Professor)
Professors
C. Robert Bagnell, Jr., Ph.D.
Dwight A. Bellinger, D.V.M., Ph.D.
Debra A. Budwit, M.D.
John F. Chapman, Dr.P.H.
Frank C. Church, Ph.D.
William B. Coleman, Ph.D.
Marila Cordeiro-Stone, Ph.D.
Cherie H. Dunphy, M.D.
Rosann A. Farber, Ph.D.
Peter H. Gilligan, Ph.D.
Virginia L. Godfrey, D.V.M., Ph.D.
M. David Goodman, M.D.
Pamela A. Groben, M.D.
Margaret L. Gulley, M.D.
J. Ed. Hall, Ph.D. (12/31/10)
Catherine A. Hammett-Stabler, Ph.D.
H. Michael Jones, M.D.
Kathleen Kaiser-Rogers, Ph.D.
William K. Kaufmann, Ph.D.
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Hyung-Suk Kim, Ph.D.
Joe N. Kornegay, D.V.M., Ph.D.
Susan T. Lord, Ph.D.
Nadia N. Malouf, M.D. (6/30/11)
Susan J. Maygarden, M.D.
Volker R. Nickeleit, M.D.
Judith N. Nielsen, D.V.M.
Howard M. Reisner, Ph.D.
John L. Schmitz, Ph.D.
Harsharan K. Singh, M.D.
Scott V. Smith, M.D.
Michael D. Topal, Ph.D.
Karen E. Weck, M.D.
Bernard E. Weissman, Ph.D.
John T. Woosley, M.D., Ph.D.
Associate Professors
Jessica K. Booker, Ph.D.
Arlene S. Bridges, Ph.D.
Georgette A. Dent, M.D.
David Eberhard, M.D., Ph.D.
Thomas H. Fischer, Ph.D. (3/12/11)
Craig A. Fletcher, D.V.M., Ph.D.
Susan C. Hadler, M.D.
Tracy M. Heenan, D.V.M.
Ruth A. Lininger, M.D.
Christopher P. Mack, Ph.D.
Melissa B. Miller, Ph.D.
Joan M. Taylor, Ph.D.
Leigh B. Thorne, M.D.
Cyrus Vaziri, Ph.D.
Maimoona B. Zariwala, Ph.D.
Assistant Professors
Araba N. Afenyi-Annan, M.D.
Claudia M. Brady, M.H.S.
Megan J. DiFurio, M.D.
George Fedoriw, M.D.
Adil Hussein Gasim, M.D.
Oleg V. Gorkun, Ph.D.
Kevin E. Greene, M.D.
Jonathon W. Homeister, M.D., Ph.D.
Peiqi Hu, M.D.
Heike Hunt, M.D.
John P. Hunt, M.D.
Karou Inoue, Ph.D. (7/31/11)
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Masao Kakoki, M.D., Ph.D.
Stephanie P. Mathews, M.D.
Christopher R. McCudden, Ph.D. (6/29/11)
C. Ryan Miller, M.D., Ph.D.
Siobhan M. O’Connor, M.D.
Kumar R. Pandya, Ph.D.
Yara A. Park, M.D.
Arlin B. Rogers, D.V.M., Ph.D.
Lori R. Scanga, M.D., Ph.D.
Dennis A. Simpson, Ph.D.
Dimitri G. Trembath, M.D., Ph.D.
Julia W. Whitaker, D.V.M.
Monte S. Willis, M.D., Ph.D.
Alisa S. Wolberg, Ph.D.
Hong Xiao, M.D.
Xianwen Yi, M.D., Ph.D.
Lecturer
Gayle C. McGhee
Instructor
Steven Holmes, B.S., M.H.S.
April E. Kemper, M.S., M.H.S.
Vincent J. Moylan, M.S., P.A. (ASCP)
Tracie L. Wagner, P.A.
Clinical Faculty (Medical Examiners)
Clay Nichols, M.D.
Deborah L. Radisch, M.D.
Samuel Simmons, M.D.
Ruth E. Winecker, Ph.D.
Faculty Emeritus
Stuart Bentley, M.D.
John D. Butts, M.D.
Myra L.Collins, M.D., Ph.D.
Robert E. Cross, Ph.D.
Frederic G. Dalldorf, M.D.
Cora-Jean S. Edgell, Ph.D.
James D. Folds, Ph.D.
Donald T. Forman, Ph.D.
Joe W. Grisham, M.D.
John E. Hammond, Ph.D.
William D. Huffines, M.D.
William W. McLendon, M.D.
James Pick, D.V.M.
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Katherine Pryzwansky, Ph.D. (Deceased 6/8/11)
Marjorie S. Read, Ph.D.
Kinuko I. Suzuki, M.D.
Jointly Appointed Faculty
Claire M. Doerschuk, M.D. (Medicine)
Ronald J. Falk, M.D. (Medicine)
Susan A. Fiscus, Ph.D. (Microbiology)
Thomas R. Griggs, M.D. (Medicine)
Nigel Mackman, Ph.D. (Medicine)
Valerie Murrah, D.M.D., M.S. (Dental)
Timothy C. Nichols, M.D. (Medicine)
Charles M. Perou, Ph.D. (Genetics)
Gloria A. Preston, Ph.D. (Medicine)
Kathleen W. Rao, Ph.D. (Pediatrics)
Allen C. Rinas, M.S. (Medical Allied Health)
Harold R. Roberts, M.D. (Medicine)
Darrel W. Stafford, Ph.D. (Biology)
James Swenberg, D.V.M., Ph.D. (Environmental Sciences and Engineering)
Young E. Whang, M.D., Ph.D. (Medicine)
Elizabeth Wilson, Ph.D. (Pediatrics)
Adjunct Faculty
William A. Ahrens, M.D. (Carolina Pathology Group)
Peter M. Banks, M.D. (Carolinas Medical Center)
Gary A. Boorman, D.V.M., Ph.D. (NIEHS)
Mark E. Brecher, M.D. (Laboratory Corporation of American)
Robert C. Brown, M.D. (Emeritus)
Shu Huey Chaing, Ph.D. (State Dept of Health and Human Services)
Jeffrey Everitt, D.V.M. (GlaxoSmithKline)
Dana M. Fowlkes, M.D., Ph.D. (Green Spring Technology)
Delores J. Grant, Ph.D. (North Carolina Central University)
Christopher Gregory, Ph.D. (Voyager Pharmaceutical)
Wendell D. Jones, Ph.D. (Constella Health Sciences/Expression Analysis)
Scott Kilpatrick, M.D. (Forsyth Medical Center)
Suzanne L. Kirby, M.D., Ph.D.
Myla Lai-Goldman, M.D. (Laboratory Corporation of America, Retired)
Chad A. Livasy, M.D. (Carolinas Pathology Group)
Roger L. Lundblad, Ph.D.
Amil E. Mandal, M.D. (Medical Specialists of St. Augustine)
Keith V. Nance, M.D. (Rex Hospital)
William R. Oliver, M.D. (East Carolina University)
Richard S. Paules, Ph.D. (NIEHS)
Dennis W. Ross, M.D., Ph.D. (Forsyth Medical Center, Retired)
Tara C. Rubinas, M.D. (Laboratory Corporation of America)
W. Eugene Sanders, M.D.
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Gary J. Smith, Ph.D. (Roswell Park Cancer Institute)
Nobuyuki Takahashi, M.D., Ph.D. (Tohoku University, Sendai. Japan)
Charles H. Wallas, M.D.
Ruth F. Walters, M.D. (Laboratory Corporation of America)
Douglas C. Wolf, Ph.D., D.V.M. (EPA)
Clinical Fellows
Edward P. Ager, Ph.D. (Microbiology)
Laura Bender, Ph.D. (Clinical Chemistry)
Dana L. Cairo Tunnell, M.D. (Hematopathology)
Eric Campenot, M.D. (Nephropathology)
Steven W. Cotten, Ph.D. (Clinical Chemistry)
Mohamed Elrefael, M.D., Ph.D. (Microbiology)
Daniel T. Kleven, M.D. (Surgical Pathology)
Yasmin Lutterbie, M.D. (Cytopathology)
Norris J. Nolan, M.D. (Surgical Pathology)
Poulomi J. Pai, M.D. (TMS)
Kristin A. Pierce, M.D. (Cytopathology)
Jonathan Privette, M.D. (Forensic)
Charles Sailey, M.D. (Molecular Genetic Pathology)
Ferrin Wheeler, Ph.D. (Cytogenetics)
Co-Chief Residents
Christopher Gordon, M.D. (PGY IV) Co-Chief Resident
Andrew Laramore, M.D. (PGY IV) Co-Chief Resident
Stacey O’Neill, M.D., Ph.D, (PGY IV) Co-Chief Resident
Residents
Dana D. Baker, M.D. (PGY III)
Natalie O. Banet, M.D. (PGY III)
Lea L. Bardy, M.D. (PGY II)
Gregory D. Bianchi, M.D. (PGY II)
Shannon A. Covey, M.D. (PGY I)
Daniel L. Duncan, M.D. (PGY I)
Christopher J. Gordon, M.D. (PGY IV)
Andrew P. Laramore, M.D. (PGY IV)
Jayson R. Miedema, M.D. (PGY II)
Stacey S. O’Neill, M.D. (PGY IV)
Jessica L. Poisson, M.D. (PGY III)
Brooke Rambally, M.D. (PGY I)
Olga Speck, M.D. (PGY II)
Sara E. Wobker, M.D. (PGY I)
Kimberly J. Woodward, M.D. (PGY III)
Research Associates
Stanislav A. Bakunov, Ph.D. (Dr. Tidwell)
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Svetlana M. Bakunova, Ph.D. (Dr Tidwell)
Paul D. Chastain, Ph.D. (Dr. Kaufman)
Stephanie M. Cohen, Ph.D. (Dr. Kaufman)
Feng Li, Ph.D. (Dr. Smithies)
Donald A. Patrick, Ph.D. (Dr. Tidwell)
Weihua Tang, M.D. (Dr. Gulley)
Postdoctoral Research Fellows
Barbara Cardinali, Ph.D. – (Postdoctoral Research Associate) – Dr. Susan Lord
Zhaokang Cheng, Ph.D. – (Postdoctoral Research Associate) – Dr. Joan Taylor
Lihong Huang, Ph.D. – (Postdoctoral Research Associate) – Dr. Susan Lord
Yukako Kayashima, Ph.D. – (Postdoctoral Research Associate) – Dr. Nobuyo Maeda
Dana Le Vine, D.V.M. – (Postdoctoral Research Associate) – Dr. Thomas Fischer
Qiang Lui, Ph.D. – (Postdoctoral Research Associate) – Dr. Richard Tidwell
Rita Marchi, Ph.D. – (Postdoctoral Research Associate) – Dr. Alisa Wolberg
John McNulty, Ph.D. – (Postdoctoral Trainee) – Dr. Cordeiro-Stone
Hind Muallem, Ph.D. – (Postdoctoral Trainee) – Dr. Margaret Gulley
Komaraiah Palle, Ph.D. – (Postdoctoral Research Associate) – Dr. Cyrus Vaziri
Noh Jin Park, Ph.D. – (Postdoctoral Research Associate) – Dr. Susan Lord
Yuliy Rozenberg, Ph.D. – (Postdoctoral Research Associate) – Dr. Christopher Mack
Maria Sambade, Ph.D. – (Postdoctoral Trainee) – Dr. Janiel Shields
Delisha Stewart, Ph.D. – (Postdoctoral Trainee) – Dr. Melissa Troester
Hirofumi Tomita, Ph.D. – (Postdoctoral Research Associate) – Dr. Nobuyo Maeda
Huili Wang, Ph.D. – (Postdoctoral Trainee) – Dr. Jonathon Homeister
Hui Yang, Ph.D. – (Postdoctoral Research Associate) – Dr. Volker Nickeleit
Xuebin Yang, Ph.D., M.D. – (Postdoctoral Trainee) – Dr. William Coleman
Yang Yang, Ph.D. – (Postdoctoral Research Associate) – Dr. Cyrus Vaziri
Zhigang Zhou, Ph.D. – (Postdoctoral Research Associate) – Dr. Joan Taylor
Graduate Students
Maria M. Aleman – (Predoctoral Trainee) – Dr. Alisa Wolberg
Jessica C. Cardenas – (Fellow Trainee) – Dr. Frank Church
Patricia Casbas-Hernandes – Dr. Melissa Troester
Dinuka M. DeSilva – (Predoctoral Trainee) – Dr. Young Whang
David A. Detwiler – Dr. Joe Kornegay
Michael L. Durando – (Predoctoral Trainee) – Dr. Cyrus Vaziri
Meghan Free – Dr. Ronald Falk
Rachel E. Goldsmith – Dr. Richard Tidwell (3/18/11)
Lance A. Johnson – Dr. Nobuyo Maeda
Kaitlin C. Lenhart – Dr. Joan Taylor
Kellie Rae Machlus – (Fellow Trainee) – Dr. Alisa Wolberg
Lantz C. Mackey – Dr. Jonathon Homeister
Troy A. McEachron – Dr. Frank Church and Dr. Nigel Mackman (1/11/11)
Matthew D. Medlin – Dr. Christopher Mack (10/25/10)
Avani A. Pendse – Dr. Nobuyo Maeda (11/9/10)
Adam D. Pfefferle – (Predoctoral Trainee) – Dr. Charles Perou
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Amanda L. Rinkenbaugh – (Fellow Trainee) – Dr. Albert Baldwin
Jessica Rodriguez – (Fellow Trainee) – Dr. Monte Willis
Aleeza J. Roth – (Fellow Trainee) – Dr. Ronald Falk
Lisa L. Samuelson – Dr. David Gerber (3/25/11)
Rupninder Sandhu – Dr. William Coleman
Kristine M. Wadosky – Dr. Monte Willis
Chih-Hong Wang – Dr. Nobuyuki Takahashi (12/31/10)
Laura Weise Cross – Dr. Christopher Mack
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RESEARCH AND SCHOLARLY ACCOMPLISHMENTS
Over the past year an excellent record of achievement in research has resulted in 254
publications of original papers and book chapters (abstracts not included). Excellence in
research and training has attracted outstanding faculty, residents, postdoctoral fellows, and
graduate students, has advanced the understanding of disease, and has enhanced the reputation of
the department and institution.
ARABA N. AFENYI-ANNAN, M.D.
Dr. Afenyi-Annan’s primary research is focused on improving transfusion care for patients with
sickle cell disease through 1) improving uniformity of blood bank practices, 2) identifying
patients at risk for red cell immunization, and 3) developing a matched- patient-donor red cell
program for pediatric sickle cell patients using African American donors from local Historically
Black Colleges and Universities (HBCUs). Currently, Dr. Afenyi-Annan is in the process of
producing manuscripts from several projects which include: a survey of blood bank practices in
North Carolina; a descriptive study of the characteristics and motivations of African American
donors from a HBCU; and a study of alloimmunization among sickle cell patients at two
Comprehensive Sickle Cell Centers. Over the next year, she will strive to complete publications
from these previous studies and seek NIH funding to support a matched patient-donor red cell
program for pediatric sickle cell patients.
ROBERT C. BAGNELL, Ph.D.
The Bagnell laboratory - Microscopy Services Laboratory - is a UNC core facility for electron
and light microscopy. The laboratory is also the Light Microscopy Core facility for the
Lineberger Comprehensive Cancer Center. Additionally, it provides clinical electron microscopy
services. During this reporting period the laboratory supported research by 341 principal
investigators from 42 departments centers and programs at UNC-CH, 2 departments at Duke, 1
department at UNC-G, 1 investigator form NIEHS, and 1 investigator from Urogenix Inc. The
total number of active laboratory clients now stands at 957. In the past 12 months the light
microscope facilities logged 11,033 hours of use, electron microscope facilities logged 2,048
hours of use and the laboratory has performed 533 electron microscopy specimen preparations.
In addition to its research role, the laboratory serves as the primary electron microscope facility
for ultrastructural clinical diagnosis for UNC Hospitals and for Dr. Charles Jennette’s renal
pathology referral service. The laboratory also serves as an alternate for UNC Hospitals clinical
electron microscopy specimen preparation service. An NIH Shared Instrumentation Grant for a
new transmission electron microscope was re-submitted with a requested amount of $422,000.
This grant was not funded. The laboratory received $100,000 in funding from the Department of
Pathology & Lab Med. to purchase an Andor FRAP system for the Olympus IX81 microscope.
The laboratory received $3,000 in funding form the Department of Pathology and Lab Med to
purchase two TMC vibration isolation tables.
Checklist of significant projects during this reporting period:
Added Klaus Haun’s Biosensor imaging technology to the lab services
Added EDAX XES to FESEM
9
Added Andor FRAP system to IX81
Created ImageJ analysis program for Biosensor FRET analysis
Created ImageJ analysis program for fibrin orientation and anisotropy
Created ImageJ plugins to streamline sensitized emission wide field FRET analysis
Presented poster of lab services at UNC Core Lab Day
Presented photographs by clients at CHANL scientific Photo Contest
Acknowledged (Steve Ray) in Cell Metabolism, Vol 13, Issue 5, pg. 517-526 “The
Microbiome and Butyrate Regulate Energy Metabolism and Autophagy in the
Mammalian Color” Scott Bultman Lab
Image analysis project: Colocalization of synaptic markers (Cendra Agulhon Ken
McCarthy Lab)
Image analysis project: Cell tracking from cardiac explants (Panna Tandon – Conlon
Lab)
Image analysis project: Measurement of FISH labeling and proximity to methyl-histone
(Mauro Calabrese – Teryy Magnuson Lab)
Image analysis project: Analysis of AAV distribution in infected cells (PH Xiao –
Richard Samulski Lab)
Image analysis project: Visualizing particle internalization by cells (Chris Luft – Joe
DeSimone Lab)
Image analysis project: Analysis of drug localization in kidney (Rachel Goldsmith – Rick
Tidwell Lab)
Image analysis project: Cell tracking in scratch assays and culture (Zhigang Zhou – Joan
Taylor Lab)
DWIGHT A. BELLINGER, D.V.M., Ph.D.
Dr. Bellinger’s research interests remain in the area of hematology and cardiovascular disease.
They have used a swine model for studying atherosclerosis for many years. They are using their
colony of familial hypercholesterolemic pigs to study the role of hyperlipidemia and insulin
resistance on atherosclerosis, wound healing and renal disease. Grant funds continue for the
maintenance of the hemophilia A and B and von Willebrand disease dogs at the FOBRL as a
National Resource. These dogs continue to be an effective model to test various gene therapies
and other strategies to correct these inherited bleeding disorders. Studies using this model have
resulted in human trials. Recent literature with a mouse KO model of factor XII indicates this
coagulation protein has importance in arterial thrombosis. They are using a naturally occurring
cat model deficient in factor XII to further investigate the role of factor XII in thrombosis and
inflammation.
JESSICA K. BOOKER, Ph.D.
Dr. Booker’s research draws from the pursuit of unusual results in clinical testing as well as the
use of her expertise in clinical molecular genetics and instrumentation to collaborate with
colleagues on a diverse range of projects. Current projects include the identification and
characterization of novel BRCA1 and BRCA2 mutations, including silent and missense sequence
variants that result in truncated proteins. A collaboration with Drs. Coleman and Funkhouser
10
utilizing DNA fingerprinting to help distinguish new primary tumors from metastases in lung
and neck cancers. As Scientific Director of the Clinical Molecular Genetics Laboratory, Dr.
Booker works closely with the research analysts and clinical fellows as they develop new assays
for acquired and inherited diseases. New assays currently under development include PML-
RARα, MLH1 promoter methylation and custom sequencing as requested by clinicians.
Assays being redesigned for improved efficiency or sensitivity include automated nucleic acid
extraction, EBV, and a Fragile X assay that will ultimately eliminate the need for Southern
blotting.
THOMAS W. BOULDIN, M.D.
For the coming year, Dr. Bouldin will continue to be very heavily involved in all aspects of
diagnostic neuropathology, providing service for surgical neuropathology, autopsy
neuropathology, the nerve biopsy service, and ophthalmic pathology. As program director of the
training program in anatomic and clinical pathology, Dr. Bouldin will also be heavily involved in
the oversight and direction of the Department’s residency program.
CLAUDIA M. BRADY, M.H.S.
Claudia Brady’s current clinical activities include instructing PGY1 through PGY4 pathology
residents and second year Pathologists’ Assistant students from Duke University in the Gross
Room. Training includes preparation of biopsy specimens through dissection, examination, and
dictation of larger and more complex surgical excisions. Emphasis is placed on thoroughness
including acquiring all relevant clinical information about the case prior to dissection, proper
triage, prioritization of caseload, and efficiency without compromising quality. Ms. Brady also
trains all levels of PGY pathology residents and medical students on all benches in Surgical
Pathology. She believes with the current faculty PAs providing all the training for the residents,
they will develop good habits and mentality with a methodical approach to their patients; the
surgical pathology specimens that they lay their hands on.
ARLENE S. BRIDGES, Ph.D.
Dr. Bridges’ current research activities involve translational drug development. Primary
research activities involve analysis of antiparasitic agents (in collaboration with Dr. Richard R.
Tidwell, Director of the UNC Consortium for Parasitic Drug Development), anti-HIV agents (in
collaboration with Dr. Ron Swanstrom, Director of the UNC Center for AIDS Research), and
anticancer nanoparticles (in collaboration with Dr. Joseph DeSimone, Director of the Carolina
Center for Cancer Nanotechnology Excellence). As Director of the ADME Mass Spectrometry
Center, her role is to provide study design assistance, bioanalytical support and data
interpretation to preclinical and clinical studies conducted by not only these three research
groups, but to other scientists at UNC and beyond.
Last year, one goal was to pay-off the amount owed on the lease-purchase of one of the mass
spectrometers in Brinkhous-Bullitt. She is proud to say that the Center has paid off all debt.
Another goal was to acquire new mass spectrometers to meet the growing demand. She recently
submitted a Shared Instrumentation Grant to the NIH and is awaiting her score. Finally, her goal
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of increasing interest in the ADME Mass Spectrometry Center is ongoing. The recent
publication of a marketing flyer in conjunction with NC TraCS and the updates to the Center
website have already helped generate new users. Dr. Bridges’ goals for the coming year are
many. First, she hopes to continue to increase interest in the ADME Mass Spectrometry Center
with continued marketing. Second, she hopes to continue to acquire new equipment, either by
donation, lease-purchase, or instrumentation grants. Third, she hopes to encourage more users to
cite the use of the Core in their publications and grant proposals. Fourth, she also hopes to use
her positions as a member of the TraCS Core Directors Committee and as Chair of the Health
Sciences Library Advisory Committee to bridge the common efforts of these two campus
organizations. Fifth, and finally, she hopes to use her position as a member of the Committee to
Develop HR Tracks for Core Facility Personnel to create a meaningful and fulfilling career path
for herself and other Core facility personnel.
DEBRA A. BUDWIT, M.D.
Dr. Debra Budwit participates in clinicopathologic studies in the areas of gynecologic pathology,
breast pathology, and cytopathology. A prior study of precursors of metaplastic breast carcinoma
has been completed, data previously presented at a national meeting, with manuscript in progress, to
be completed this year. Other ongoing collaborative projects in which she participates includes
evaluation of current medical management protocols for patients with endometrial hyperplasia/well
differentiated carcinoma, follow-up of and evaluation of management strategies for young patients
with cervical intraepithelial neoplasia 2 (CIN 2), the utility of sentinel lymph node biopsy in
patients with breast cancer status post neoadjuvant chemotherapy, and the utility of sentinel lymph
node biopsy in patients with endometrial cancer.
JOHN F. CHAPMAN, Dr. P.H.
Dr. Chapman’s responsibilities and activities at UNC continue to be primarily associated with
service functions in Point of Care Testing at UNC Healthcare and the Core Laboratory section of
McLendon Clinical Laboratories. Point of Care Testing remains an active and expanding
activity throughout UNC Healthcare. Since beginning phased retirement, Dr. Chapman has also
remained an active investigator in clinical trials sponsored by in vitro diagnostic (IVD)
manufacturers, recently completing an external validation trial for automated HIV and Hepatitis
applications. In general, he plans to remain actively engaged in laboratory and point of care
service functions, as well as any translational research activities that may arise, during the
remainder of his appointment here at UNC.
FRANK C. CHURCH, Ph.D.
The research area of Frank Church, Ph.D. is concerned with proteases and their inhibitors in
human biology and in various disease processes (including thrombosis/vascular biology and
tumor cell migration/invasion/signal transduction). His laboratory has an extensive interest in
the biological principles of proteolysis and they take a two-pronged approach to research: In the
first approach, Dr. Church’s lab performs structure to activity studies with heparin-binding
serpins (serine protease inhibitors; heparin cofactor II, protein C inhibitor, antithrombin, and
plasminogen activator inhibitor-1) and the serine protease thrombin and activated protein C.
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They have made substantial progress in identifying specific residues in these serpins that are
important for glycosaminoglycan binding and for protease recognition. In the second approach,
they are applying basic biological techniques (in vivo, ex vivo, and in vitro) to investigate newly
emerging principles of proteases in biological processes, especially in venous thrombosis and
breast cancer. Part of this research is directed at using mouse models of cancer and thrombosis,
in an attempt to better understand Trousseau’s Syndrome, the link between venous thrombosis
and cancer by understanding the roles of plasminogen activator inhibitor-1 (PAI-1), urokinase,
PAR-1 and PAR-2, and factor VIIa/tissue factor. Part of this work is trying to use mice
genetically altered in their expression of p16INK4a
and its relationship to senescence/aging and
venous thromboembolism. Finally, they are focused on understanding the role of PAI-1 in breast
cancer and the microenvironment (breast adipocytes) and the signaling systems supported by
PAI-1 and by PAI-1-urokinase complex.
WILLIAM B. COLEMAN, Ph.D.
Dr. Coleman’s laboratory is focused on elucidation of epigenetic mechanisms underlying human
breast cancer development by examining breast cancers that exhibit high rates of gene silencing
due to hypermethylation defects (which are ER-negative) and those that lack methylation-
dependent gene silencing (which are ER-positive). They found that ER-negative breast cancers
exhibit a hypermethylation defect characterized by overexpression of DNMT3b protein and
elevated DNMT activity leading to concurrent aberrant methylation of numerous genes, and that
this group significantly corresponds (~70%) to the basal subtype of breast cancer. Recent efforts
have characterized the role of several microRNAs in the post-transcriptional regulation of
DNMT3b. Ongoing efforts will continue to examine the molecular basis for the
hypermethylation defect and investigate strategies for targeting the epigenome in the treatment of
these breast cancers.
MARILA CORDIERO-STONE, Ph.D.
Dr. Cordeiro-Stone’s research program is focused on molecular mechanisms underlying the
responses of human cells to DNA damage induced by solar radiation. Normal human fibroblasts
and melanocytes, as well as various melanoma cell lines, are used as experimental model
systems. Cultured cells are exposed to UVB and UVA wavelengths represented in ambient
sunlight and their responses compared at the levels of DNA replication and repair, the intra-S
checkpoint, and induced mutagenesis. Such comparison is possible through the determination of
actual densities of induced DNA lesions as more accurate indices of molecular dose than the
incident fluence of radiation. The etiology of skin melanomas and carcinomas are strongly
connected to sun exposure, but the mechanistic roles of short (UVB) or longer (UVA)
wavelengths are not clearly understood. This information is essential for the development of
guidelines aiming at skin cancer prevention. Studying the function(s) of proteins involved in the
regulation of DNA replication and the network of pathways that protect the human genome from
genotoxic effects of DNA damage is essential for a better understanding of the pathogenesis of
skin cancers with an environmental etiology.
13
GEORGETTE A. DENT, M.D.
Dr. Dent’s research is focused on the role that enrichment activities play in the career
development of medical students. She is particularly interested in the impact of international
programs and graduate study in public health on educational outcomes. She is collaborating with
the recently established School of Medicine Office of International Activities to investigate the
impact of international service and research programs on medical student education. A
manuscript describing some of this work was published in Academic Medicine last year. In
collaboration with the School of Public Health, she is investigating the impact of training in
public health on physician practice patterns and career satisfaction. A preliminary report of this
work was published in the April 2008 volume of Academic Medicine. Goals for the future
include extending the physician survey to additional alumni using social media.
MEGAN J. DiFURIO, M.D.
Dr. DiFurio’s areas of subspecialty interest include cytopathology, and gynecologic and breast
surgical pathology. She has provided clinical services as well as participated in clinicopathologic
and translational research projects in these areas over the past year. Dr. DiFurio presented an
abstract on a strategy for improving Pap test unsatisfactory rates at the annual American Society
of Cytopathology meeting in November 2010 and participated with the Division of Gynecologic
Oncology at UNC in a round table journal club discussion which has recently been accepted for
publication in the American Journal of Obstetrics and Gynecology. Other current research
projects include the following: (1) collaboration with Dr. Bae-Jump in Division of Gynecologic
Oncology in the study of the effects of obesity and anti-diabetic medications (e.g., Metformin)
on development of ovarian carcinoma in a mouse gene knock-out model, (2) collaboration with
Dr. Van Le in Division of Gynecologic Oncology in the study of the optimal size of margins in
women with squamous cell carcinoma of the vulva, (3) collaboration with Dr. Rahangdale in the
Department of OB/GYN and Dr. Smith in the School of Public Health in the collection and
archiving of cytology and biopsy materials from women with cervical dysplasia with the future
hope of looking for factors to help predict which women will go on to develop invasive cervical
carcinoma, (4) collaboration with Dr. Brewster on the Division of Gynecologic Oncology in the
study of the possible presence of microbiotic organisms ion the upper GYN tract and the possible
relationship to carcinogenesis, and (5) collaboration with Dr. Hackman in the Department of
Otolaryngology/Head and Neck Surgery in the study of the accuracy of ultrasound guided FNA
in detecting persistent disease in lymph nodes of patients with head and neck squamous cell
carcinoma after definitive chemotherapy. In the next year, Dr. DiFurio hopes to continue with
these research interests especially expanding her work in the area of cervical and vulvar
neoplasia. She is also looking forward to continuing to help Dr. Lininger to expand the
gynecologic and breast pathology outside consultation service. Other goals include continuing to
provide at least 4 lectures/slide teaching sessions for the pathology residents and fellows and
becoming more involved in the Reproductive Medicine course for the UNC Medical Students.
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CHERIE H. DUNPHY, M.D.
Dr. Dunphy’s research involves the development of distinguishing markers of diffuse large B-
cell lymphoma, double-hit lymphomas, and Burkitt lymphoma by full gene expression profiling
with extrapolation of immunohistochemical markers and correlation with clinical outcomes. She
has established several collaborations:
1. with Dr. Ken Young, Wisconsin, regarding gene expression profiling of diffuse large B-
cell lymphoma.
2. with Dr. Sandeep Dave, Duke University, regarding gene expression profiling of diffuse
large B-cell lymphoma and Burkitt lymphoma.
3. with Dr. Kristy Richards, UNC, regarding diffuse large B-cell lymphoma.
4. with Dr. Kristy Richards, UNC, and Matthew Breen, NC State University, regarding
FISH of dog lymphomas.
Dr. Dunphy will continue to work as an author/editor on several projects:
1. Editorship/Authorship of E-Medicine Pathology textbook for liquid and solid
Hematopathology/Hermatology;
2. Authoring and editing textbook entitled Frozen Section: Lymph Node;
3. Authoring Chapter regarding Hematopathology for Medical School textbook;
4. Authoring and editing textbook entitled An Atlas of Neoplastic Hematopathology;
5. Analysis of the Significance of CD200 Expression in Plasma Cell Dyscrasias; and
6. Authoring and editing Mini-Series Topic of Myeloproliferative Neoplasms and
Myelodysplastic/Myeloproliferative Overlap Neoplasms: Current Diagnosis and Therapy
Authoring and Editing of Topics recently presented at the Society for Hematopathology
Symposium at USCAP, 2010 entitled: Atypical and Reactive Lymphoid Proliferations
Mimicking Malignant Lymphoma to be published in Seminars of Diagnostic Pathology.
DAVID EBERHARD, M.D., Ph.D.
Dr. Eberhard’s laboratory space has been under renovation since his 4/17/2011 start date (He has
a temporary office in the interim). The lab should be ready for use in September 2011. He has
been purchasing equipment for the new lab and is posting job requirements for a Research
Associate laboratory manager and a Research Technician. His ongoing activities include:
“Director of Pre-Clinical Molecular Pathology” for the Cancer Genomics efforts in the
Lineberger Cancer Center, develop pathology QA and tumor sample workflow (LIMS and
physical sample workflow) within the LCCC, participate in clinical trial protocol development,
advise in clinical development of new assays, and nanostring analysis core service. His plans and
goals include: continuing and expand ongoing activities described above. Implement BSP LIMS
and digital pathology for sample QA, initiate original and collaborative research focusing on
solid tumor heterogeneity and how it relates to response and recurrence after therapy, digital
pathology analytic approaches to define and quantitative heterogeneity, methods to facilitate
molecular and genomic analyses of tumor cell subpopulations.
15
ROSANN A. FARBER, Ph.D.
Dr. Farber plans to write and submit a manuscript on work previously completed by members of
her lab on instability of CG- and AT-repeat sequences in normal fibroblasts and mismatch-
repair-deficient cancer cell lines.
GEORGE FEDORIW, M.D.
Dr. Fedoriw’s research, in collaboration with Dr. Sarantopoulos (Department of Medicine), is
primarily focused on further defining the role of the B cell activating factor (BAFF) in chronic
graft versus host disease (cGVHD) after allogeneic bone marrow transplantation. Findings from
his work were recently presented a Keystone meeting in spring of 2011. He has also investigated
pathways of B cell activation in HIV associated lymphomas (funded through the UNC Center for
AIDS Research), and is working to identify relevant B cell subsets in human cGVHD (funded
through the NC TraCS Institute). Dr. Fedoriw also actively provides research support for
collaborators in the Lineberger Cancer Center and the School of Pharmacy. His goals for the
upcoming year include applying for additional funding for independent research work.
CRAIG A. FLETCHER, D.V.M., Ph.D.
Dr. Fletcher will continue to provide management and veterinary clinical care for the research
animals on campus as his primary function. With the new animal facilities opened on campus,
the mouse census will more than double, which will significantly increase the case load. It is
important for DLAM to be prepared to negotiate resources to meet current and future demands.
He would like to enhance his negotiation building skills and sharpen his political acumen.
Strategic planning is a tool for the university to find its competitive advantage and place within
this dynamic environment. He would like to further enhance his skills in thorough strategic
planning and plan execution to meet the needs of the DLAM. His acceptance into the ULEAD
program will continue to develop these skills. His collaborative research programs involving
study of pathogenesis of cardiovascular diseases and platelet biology will continue.
WILLIAM K. FUNKHOUSER, M.D., Ph.D.
Dr. Funkhouser collaborates as a funded Pathologist with the Baric research group on lung
morphologic changes in SARS respiratory virus vaccination models in mice.
Dr. Funkhouser collaborates with Dr. Olshan in Public Health Epidemiology on followup studies
derived from the 5 year funded CHANCE study on risk factors for head and neck carcinoma in
the state of North Carolina. Dr. Funkhouser collaborates with Dr. Hayes at the LCCC on
projects related to inter-observer reproducibility of morphologic diagnosis of non-small cell lung
carcinoma (NSCLC) and molecular subsets of the different types of NSCLC. These projects are
attempting to define more accurate criteria for making the diagnoses of the different types of
NSCLC, and identifying molecular subsets with statistically different natural histories or
responses to therapies. Dr. Funkhouser collaborates with Dr. Coleman of the DPLM on two
projects. The first is to define molecular methods for determination of neoplastic clonalityunique
to each neoplasm in a given individual. Such a method would allow distinction of two
morphologically similar neoplasms from one another, e.g. distinguishing a solitary pulmonary
16
metastasis from a new lung carcinoma. The second is a technical project, with a goal of creating
a durable, reusable solid phase cDNA library in a microscale bioreactor.
PETER H. GILLIGAN, Ph.D.
There are two areas that will be pursued in the coming year. Studies to understand the
microbiome of chronic lung disease in cystic fibrosis patients are ongoing. In the coming year,
the focus of the work will be the role of anaerobic bacteria and on viruses in this process. The
second area of investigation will be in the development of novel diagnostics for the detection of
Clostridium difficile infections. Novel amplification technologies to detect C. difficile toxin
genes will be evaluated.
VIRGINIA L. GODFREY, D.V.M., Ph.D.
Dr. Godfrey will continue to provide collaborative pathology evaluations for colleagues in the
Medical School faculty, particularly members of the Lineberger Comprehensive Cancer Center.
Recent and continuing projects include morphologic evaluations of: 1) pig models of
atherosclerosis and Type II diabetes (Nichols), 2) Brg 1 mutant mice (Bultman), 3) dog models
of hemophilia (Nichols), and 4) mouse models of choline deficiency (Ziesel). She will assist in
characterization of new mouse models through the interactions with the National Gnotobiotic
Rodent Resource (B Sartor), the Mutant Mouse Regional Resource Center (MMRRC) at UNC
(Magnuson), and the Collaborative Cross (Pardo Manuel de Villena).
MICHAEL DAVID GOODMAN, M.D.
Dr. Goodman’s primary project: Perou Breast Case Database – review of breast pathology with
tissue sample selection for TMA and DNA acquisition. He is also serves as consultant in the
Translational Pathology Laboratory: Pathologist assigned to project case review. He provides
additional sporadic case review as needed.
OLEG V. GORKUN, Ph.D.
Dr. Gorkun is a fibrinogen expert and protein chemist. He has been using biochemistry and
biophysics approaches to study fibrinogen structure and molecular mechanisms of its
functioning. His current research interests lies in the area of biomechanical properties of
proteins. He has developed close partnership with labs of Dr. Schoenfisch (Department of
Chemistry) and Dr. Falvo (Department of Physics). With the Schoenfisch lab he pursues studies
of fibrinogen mechanical behaviors on the single molecule level. He also expanded his research
into the area of bacteria adhesion to plasma protein including fibrinogen. Together with
Schoenfisch lab they developed an analytical method based on Atomic Force Microscopy to
study interactions of plasma proteins and protein-adhesins expressed in bacteria wall. The two
advantages to this method: (1) they can study protein interaction on the single molecule level,
and (2) protein interactions are studied under conditions similar to dynamic environment of
blood flow, where interactions are establish and survive under continuous stress of mechanical
forces, generated by flow shear. Together with Dr. Falvo he investigates the molecular
mechanism by which fibrin polymers (fibers in the fibrin clot) maintain their extremely high
17
extensibility and strain hardening. In course of studying permeability of fibrin fibers they
unexpectedly discovered that fibrin fibers are permeable to such large protein as activated FXIII
(6x18 nm ellipsoid). These findings imply that the fibrin fiber structure may be dynamic with
large pores constantly shifting in the fiber.
KEVIN G. GREENE, M.D.
Dr. Greene is currently a subinvestigator in a clinical trial, and associated extension study,
evaluating the efficacy and safety of everolimus in liver transplant recipients. He is also currently
collaborating with a radiation oncologist to create digital 3D reconstructions of liver tissue to
evaluate the relationship between tumor and radioactive isotope beads. Additional similar
projects are anticipated for next year.
PAMELA A. GROBEN, M.D.
Dr. Groben enjoys collaborating with colleagues on translational research projects and projects
that require her surgical pathology skills. She is currently working with Nancy Thomas from
Dermatology looking at cutaneous melanoma. She reviews slides on melanomas and other
melanocytic lesions, record the histologic features and use laser capture micro-dissection to
acquire melanoma cells in small lesions so that mutations for BRAF mutations and DNA-
methylation profiles can be determined. (See Thomas, “Tandem BRAF mutations in primary
invasive melanoma”, 2007; Thomas, “Number of nevi and early life UV exposure are associated
with BRAF-mutant melanoma”, 2007; Thomas, “Relationship between germline MC1R variants
and BRAF-mutant melanoma in a North Carolina population based study”, 2010; and Conway,
“DNA-methylation profiling distinguishes malignant melanoma from benign nevi”, 2011 in the
publication section). Several studies are ongoing and histologic review and laser capture to
acquire DNA continues to be a requirement.
MARGARET L. GULLEY, M.D.
Dr. Margaret L. Gulley’s research is aimed at 1) understanding the molecular basis of Epstein-
Barr virus (EBV)-related malignancies, and 2) developing novel laboratory tests to assist in
diagnosis and management of affected patients. In the past year there has been substantial
progress towards these goals. They are validating two gene expression profiling systems to
apply in paraffin embedded tissue, blood, plasma, serum and other specimen types. They used
well-characterized paraffin embedded cell lines for analytic validation, and they are currently
collecting data on primary specimens from patients with cancer, precursor lesions, and non-
neoplastic controls to demonstrate clinical utility in categorizing disease using patterns of RNAs
(coding and non-coding). In work of a more general nature, they teamed with researchers
campus-wide to improve biobanking services for local investigators. They validated novel
molecular assays for use in clinical trials and in routine patient care. This work builds on basic
science discoveries and translates them to the clinical realm, reinforcing the important role of
pathologists in advancing medical practice using modern molecular tools. In the coming year,
they seek more funding for assay validation projects and clinical trials to gather clinical evidence
of efficacy. They will continue to maximize productivity of local clinical investigators by
making tissue/lab/pathologist resources available for team science.
18
SUSAN C. HADLER, M.D., M.S.
Dr. Susan Hadler’s efforts in the Medical School are centered around teaching and curriculum.
She is involved in teaching 1st, 2
nd and 4
th year medical students in multiple courses, as well as
Pathology and Toxicology graduate students and Physical Therapy graduate students. She
serves on a number of medical school curriculum related committees. Her efforts in the Dental
School are also centered on teaching; she teaches 1st year dental students in multiple courses.
She also serves on the Dental School’s admissions committee.
CATHERINE A. HAMMETT-STABLER, Ph.D.
Dr. Hammett-Stabler’s focus is in the improvement of clinical laboratory support of pain
management and addiction. During the past year, she has systematically determined previously
undocumented cross-reactivity of the immunoassay-based drug screens used by the MCL Core
Laboratory for commonly prescribed or abused drugs (or their metabolites). In addition, she
continues collaborations with Drs. Robert Aris and Margaret Gourlay. Her work with Dr. Aris
investigates the uptake, metabolism, and mechanism of action of immunosuppressant drugs by
and within human tracheobronchial epithelial cells. The work with Dr. Gourlay continues
assessing the relationship between osteoporosis and various biomarkers. Work conducted this
past year suggests the previously reported association between higher concentrations of follicle
stimulating hormone (FSH) and lower bone mineral density (BMD) reflects an indirect effect of
body composition (lean vs fat mass) on FSH, not a direct effect of FSH on BMD.
TRACY M. HEENAN, D.V.M.
Dr. Heenan continues to provide excellent service to animal research community, ensuring
humane animal care and use, facilitating the application review process, providing exemplary
training of research personnel, and conducting fair and thorough investigations of animal welfare
concerns and noncompliance while still working to establish rapport with researchers and
fostering animal research. The necessity of providing fair and thorough customer service is one
of Office of Animal Care and Use (OACU) guiding principles. With escalating compliance-
related responsibilities, such as increased investigator-managed animal facilities as well as offsite
facilities, it will most likely be necessary to add an additional T/C Coordinator position in the
next five years. OACU, like everyone on campus, has had to plan for budgetary cuts. The office
has made monumental strides in reducing the amount of paper as well as the associated costs by
moving to an electronic review process. They have eliminated the voluminous paper packets and
members now review applications and the 1000 page semi-annual facility report electronically.
During the next several years, the office will fully implement the position responsible for Grant
Application/IACUC Application congruency, which has recently been approved. This will be a
process requiring cooperation and buy-in from research faculty. During the next five years the
office will continue to educate and advise faculty, students, research personnel, IACUC, Division
of Laboratory Animal Medicine (DLAM) personnel, and Department of Environment Health and
Safety (EHS) representatives regarding proper animal care and use policies and practices. Dr.
Heehan will continue to serve as an integral link between the IACUC and the Office of the Vice
19
Chancellor for Research (VCR), DLAM, EHS, and the University Employee Occupational
Health Clinic and will work to enhance all levels of communication between these groups.
STEVEN HOLMES, M.H.S.
Steven Holmes plans to continue to instruct residents, Duke PA students and the occasional
medical student in pathology and gross anatomy. He will help the residents formulate how to
construct a concise/coherent gross dictation. Moreover, these dictations are legal documents;
therefore, it is important to handle every specimen (simple or complex) with the same
professionalism. He will delve into other aspects of the laboratory such as frozen section room
coverage, ordering of supplies, and communication with appropriate personnel with respect to
the troubleshooting of laboratory equipment.
JONATHON W. HOMEISTER, M.D., Ph.D.
The research of Jonathon Homeister, M.D., Ph.D. has two major goals. The first is to utilize
leukocyte lineage-specific transgenic gene expression and leukocyte lineage-specific gene
targeting in murine experimental models to investigate α(1,3)-fucosyltransferase (FUT) gene
function in the development of atherosclerotic cardiovascular disease. They are using these mice
and other mice made deficient in FUT-IV and FUT-VII in all tissues to define a role for the
selectin adhesion molecules and their fucosylated ligands in the development and progression of
atherosclerosis. These mouse strains will be used to continue their studies that define the
selectin-dependent contribution of several leukocyte lineages to the atherosclerotic disease
process. The second goal is to determine the mechanisms whereby the FUTs regulate hemostasis
and thrombosis. These studies are determining the mechanisms whereby fucosylation of selectin
ligands and/or other blood molecules alters coagulation and thrombosis. These studies also
utilize the mouse strains described above to modulate generalized and leukocyte lineage-specific
FUT expression.
PEIQI HU, M.D.
Dr. Hu’s current research, in collaboration with Dr. Jennette, is focused on 1) investigating
involvement of genetic factors in pathogenesis of ANCA induced glomerulonephritis. They
recently found that anti-MPO IgG caused different severity of ANCA disease in different strains
of mice, which mimics disease variation in ANCA patients. Next, they will test the ANCA-
mediated disease induction in eight different founder strains and try to explore genetic basis
responsible for variations in severity of disease, and hopefully to find out candidate genes and
their protein products that involved in pathogenesis of the disease. 2) trying to identify specific
epitopes that are targeted by pathogenic anti-MPO IgG. They have already created recombinant
mouse/human MPO chimeric molecules and will use them to detect the portion of mouse MPO
that is responsible for the disease induction. 3) generating PR3-ANCA disease mouse model.
Success with this model would advance their understanding of mechanism of PR3-ANCA
disease.
20
HEIKE HUNT, M.D.
Even though she was on leave for half year, Dr. Hunt has continued her research efforts and has
been active teaching graduate student labs and lectures, and advancing her microRNA
investigations. Unfortunately, her small grant application with the University Research Council
entitled “MicroRNA expression profiles associated with prognosis and therapeutic outcome in
human Hepatitis B-associated Hepatocellular Carcinoma” was not funded. Nevertheless, she has
started a project investigating the microRNA expression in those hepatocellular carcinomas that
arise in hemochromatosis patients. This has not previously been reported in the literature, so it
should yield exciting new data. She is collaborating with a lab in Florida and they have started to
extract the RNA and will hopefully have initial data on the microRNA content in the next 2
weeks. She is also continuing to work with Derek Chiang, PhD, whose specialty at UNC is gene
sequencing, especially in liver diseases and liver tumors. Currently, she is scoring
immunohistochemical stains of his mice livers for beta-catenin and 5-hmc. A manuscript based
on their work from about a year ago has been submitted for publication. She is looking forward
to returning to the office every day, so she can increase the velocity of her projects and be more
accessible for her collaborators. Dr. Hunt’s research goals for the next half year include
preparing a Grand Rounds talk entitled “HepPar and the deathly hairpins”, which will summarize
the research results of her career thus far, spanning work performed in 3 continents, and
preparing the manuscript of the microRNA expression profile in hemochromatosis-related HCC.
JOHN HUNT, M.D.
Although occasionally consulting on evaluation of immunohistochemical stains by various
groups (e.g., Dr. Coleman’s laboratory, or Dr. DeMore), there are no current formal ongoing
clinical, translational, or basic research activities. Goals include completion of two or three book
chapters (frozen section pathology of lymph nodes (2 chapters), molecular basis of lymphoma
(one chapter) but no other research activities are planned.
ADIL HUSSEIN GASIM, M.D.
Dr. Hussein Gasim’s activities include, research & clinical nephropathology cases, specially
electron microscopy and immunofluorescence of the renal biopsies. He also provides instruction
of medical students in UNC School of Medicine in nephrology and nephropathology. He is also
involved in the following ongoing research activities:
1. C4d staining in the glomerular capillaries of renal allograft biopsies.
2. The significance of peritubular capillaries lamination, in renal allograft biopsies.
3. Virtual versus conventional light microscopy in renal pathology: Comparative survey.
The plan for the coming year is to focus more on research activities.
J. CHARLES JENNETTE, M.D.
A major portion of Dr. Jennette’s recent basic research has utilized an animal model of ANCA
disease discovered in his laboratory that is induced by i.v. injection of mouse anti-
myeloperoxidase (anti-MPO) IgG antibodies or anti-MPO lymphocytes into mice that is
mediated primarily by activation of neutrophils. Activation of the alternative complement
21
pathway is critically involved in the pathogenesis of disease in this model. ANCA-activated
neutrophils release factors that activate complement, which in turn primes neutrophils for further
activation by ANCA. These effects and other ANCA-mediated pathogenic events depend on
generation of C5a by alternative pathway activation and on engagement of C5a receptors on
neutrophils. Blockade of this critical pathogenic step abrogates disease induction, which suggests
a possible novel therapeutic strategy in humans. Recent observations show that another receptor
for C5a in addition to C5aR, i.e. C5L2, is an inhibitory rather than an activating receptor for
inflammation. Knock out of this receptor worsened anti-MPO induced disease. Ongoing studies
using this mouse model as well as patient samples indicate that Fc gamma receptors are involved
in pathogenesis and in the modulation of disease phenotype. Genetic variations among mouse
strains have a dramatic influence on disease severity. Genomic studies are underway to identify
the genes responsible for these differences in disease severity. Candidate genes or genetic
polymorphisms are being studied in parallel in patients with ANCA disease. Bone marrow
transplant studies of anti-MPO disease have demonstrated that these genetic influences act
primarily on and through bone marrow derived cells. Experiments are underway to assess the
induction of disease by antibodies against specific MPO sense and anti-sense peptides.
Pathogenic epitopes are being mapped using human-mouse chimeric molecules. In the mouse
model, antibodies against recombinant mouse MPO are pathogenic but antibodies against
recombinant human MPO are not. The Lab is preparing chimeric molecules from clones that
have various segments of the murine MPO gene mixed with segments of the human MPO gene.
These studies are demonstrating that some but not all of these chimeric molecules will induce
antibodies that cause disease, thus identifying the portion of the MPO molecule that is the target
of pathogenic antibodies.
HARVEY MICHAEL JONES, M.D.
Dr. H. Michael Jones’ research activities are primarily in the history of medicine. He has
submitted a chapter for an upcoming publication to be entitled The Persisting Osler, Vol. 4. The
work is scheduled for publication in the fall of 2011. This work relates some of the interactions
between Sir William Osler (the first chief of medicine at the Johns Hopkins Hospital and School
of Medicine), North Carolina physician E. J. Wood of Wilmington, and Joseph Goldberger (who
defined the cause of pellagra). He is chair of the local arrangements committee for the American
Osler Society, the annual meeting of which will be held in Chapel Hill in April 2012. He is
participating in the preparation of a video for that event emphasizing the role of mentoring in
medical education. It will highlight some of UNC’s best teachers and feature an interview with
Dr. William McLendon about the role of W. B. MacNider at UNC.
Dr. Jones also functions as a research support pathologist with the Tissue Procurement
Laboratory, assisting in the selection and quality assurance for pathologic materials that form the
basis of molecular and genetic studies for other principal investigators across the campus.
KATHLEEN A. KAISER-ROGERS, Ph.D.
Research in the clinical cytogenetics laboratory involves the use of both traditional and
molecular cytogenetic techniques including both fluorescence in situ hybridization (FISH) and
single nucleotide polymorphism (SNP) microarray analysis to identify and characterize
22
rearrangements in their patient population. In addition to identifying copy number gains and
losses in the human genome, the SNP array technology also enables them to identify long
continuous stretches of homozygosity associated with uniparental disomy (the inheritance of
both copies of a chromosome, or chromosome part, from a single parent) and consanguinity
(matings between closely related individuals), both of which can result in genetic disease. While
the microarray technology is currently being used to detect constitutional chromosome
abnormalities, during the next year they hope to validate the SNP microarray technology for the
detection of acquired chromosome changes in some of their oncology specimens. The laboratory
also continues to serve as a resource for researchers on campus who are interested in applying
cytogenetic techniques to their research projects
MASAO KAKOKI, M.D., Ph.D.
Dr. Kakoki has recently generated mice with low expression (approximately 10% mRNA of
wildtype) of TGFβ1 by the targeted replacement of the 3’ untranslated region (UTR) of Tgfb1
with that of Fos on a pure C57BL/6 genetic background. He has found that the TGFβ1
hypomorphic mice develop hypertension, impaired diuresis/natriuresis with normal creatinine
clearance, primary aldosteronism, aneurysm in the ascending aorta and dilated cardiomyopathy.
All of these unexpected results elucidate important novel roles of TGFβ1 in the cardiovascular
homeostasis (manuscript in preparation). He plans to study the mechanisms whereby low TGFβ
levels cause these cardiovascular diseases and is now asking grant supports for this purpose.
Next, he studied that the effect of the genetically low levels of TGFβ1 on diabetic nephropathy,
using Akita male mice, a mouse model of type I diabetes. The TGFβ1 insufficiency does not
significantly change glucose or insulin levels in the plasma, but it reverses urinary output of
albumin and renal fibrotic changes in Akita mice. The low levels of TGFβ1 also decrease urinary
excretion of water and glucose in Akita diabetes, which in turn suppress the intake of food and
water as well. These results suggest that TGFβ1 plays a major role in developing many features
of diabetes including albuminuria, polyuria, glucosuria, polydipsia and bullimia. He plans to
study the mechanisms for these findings, hypothesizing that the uptake of sodium, glucose,
albumin is increased by activation of Na+,K
+-ATPase by low levels of TGFβ1 in proximal
tubules. Since the targeted Tgfb1low
allele is convertible into high expressing Tgfb1high
allele, he
plans to phenotype TGFβ1 globally and conditionally hypermorphic mice as well.
DAVID G. KAUFMAN, M.D., Ph.D.
He is studying the temporal order of replication during the S phase using high throughput DNA
sequencing. Initial studies are aimed at validating the methodology by verifying the location of
the DNA replicated in the first hour of the S phase as found in his earlier studies involving the
cloning of the early replicating DNA. He is also attempting to use this methodology to identify
the sites of the origins of DNA replication from throughout the genome. He is also using studies
of extended single DNA fibers to discover the locations of origins of DNA replication in early
replicating regions. The single DNA fiber technique has also been applied to the quantification
and location of sites of DNA damage in DNA and the alterations of DNA replication resulting
from DNA damage. Efforts are ongoing to develop automated techniques for analysis of
fluorescently-labeled newly-replicated DNA fibers and his collaborators and he are patenting this
23
process and seeking commercial partners of other funding for its further development. There are
studies planned to evaluate the translational application of this process to assess “cell stress” in
cells obtained from patients. In parallel studies, techniques have been developed to assess
extended chromatin fibers and demonstrate sites of DNA replication or DNA damage together
with the ability to localize multiple proteins simultaneously at these sites of replication or repair.
These studies have been shown to provide the temporal pattern of association of these proteins in
addition to their localization
His other studies of reconstructed human endometrial tissue in co-culture have a more
clinical/translational focus. Co-cultures of endometrial cells structured to resemble normal
endometrium and endometrial intraepithelial neoplasia (EIN) have been used to assess
differences in gene expression in the two states using cDNA microarrays. Immunohistochemical
studies using antibodies selected based on the findings from microarray analysis are being
assessed on tissue microarrays of normal, hyperplastic, EIN and invasive neoplastic
endometrium. The goal of these studies is to develop an immunohistochemical test that can aid
in the distinction between benign endometrial hyperplasias and EIN, which can be a difficult
diagnosis to make based only on H&E stained slides. He has plans for preclinical studies to
assess methods to modulate the chemotherapeutic effectiveness of arsenic trioxide (As2O3) in
advanced endometrial cancer. These studies will use co-cultures to assess the status of a
pathway providing protection against the effects of As2O3 and determine whether this pathway
can be inhibited to enhance the effects of As2O3. He also has plans to determine whether the
process of implantation of the human embryo in the endometrium can be simulated by adjusting
the hormonal status of the endometrial co-cultures and determining whether they provide an
appropriate surface for the attachment of embryonic human trophoblastic cells. If this model can
be validated it may prove useful for studies of certain forms of female sterility and aid in
development of strategies for their correction.
WILLIAM K. KAUFMANN, Ph.D.
Dr. Kaufmann’s research is focused on the mechanisms of chromosomal instability in melanoma.
Studies are monitoring components of the DNA damage response in normal human melanocytes
and melanoma cell lines to identify protective elements of DNA metabolism that function in
normal melanocytes but are degraded in melanomas. The p53-dependent G1 checkpoint response
to DNA damage was found to be degraded in 64% of melanoma cell lines, the p53-independent
G2 checkpoint response was degraded in 33% of melanoma lines, and the intra-S checkpoint
response to UV-induced DNA damage was found to be fully functional in all melanoma lines
tested. This result may speak to the fact that the intra-S checkpoint is indispensible and performs
an essential function during DNA replication to stabilize DNA replication forks that are stalled at
natural replication barriers. Future studies will focus on targeting this checkpoint for selective
killing of cancer cells. Goals in the upcoming year are to renew funding for their work.
APRIL KEMPER, M.H.S.
Currently, Ms. Kemper is providing one-on-one training to 1st year pathology residents and
overseeing grossing by the 2nd
, 3rd
, and 4th
year residents. She believes her love of pathology,
enthusiasm and systematic approach to grossing has helped to provide a great learning
24
environment. This year they (the PAs) are conducting bimonthly and monthly gross conferences.
She hopes that the conferences will provide the residents with the opportunity to learn gross
pathology in a fun interactive environment. She also plans to continue working with her co-
workers to reduce turnaround time, minimize labeling and grossing errors, reduce the number of
cassettes submitted per case and implement new methodologies to aid in physician training and
patient care.
HYUNG-SUK KIM, Ph.D.
Dr. Kim continues to study complex genetic diseases using various animal models that were
generated by gene targeting techniques. The resulting animals show genetic factors to play a key
role in disease. To understand homeostatic response to genetic changes, Dr. Kim developed
molecular phenotyping procedures based on gene expression using high-throughput real time
RT-PCR methods. His results demonstrated the method’s power for recognizing subtle
phenotypic changes in animals even with minimal genetic differences. Using this powerful
technique, Dr Kim (as a core director of gene expression study) has been collaborating with
researchers in many fields, mainly cardiovascular disease with Drs. Smithies and Maeda group,
kidney problems with Drs. Arendshorst, Coffman (Duke Univ.), Williams (Temple Univ.) and
Sharma (UCSD). Heart failure with Dr. Meissner, neurogical disease with Dr. O’Connor
(UCSD). His goal is more development for characterization of animal models, such as
molecular and physiological phenotype.
JOE N. KORNEGAY, D.V.M., Ph.D.
Dr. Kornegay plans to continue his own hypothesis-driven projects, focusing particularly in three
areas: (1) Use of autologous cells that have been corrected using a lentivirus-minidystrophin
construct to treat GMRD dogs. In the context of these studies, they have developed a strong
collaboration with Nancy Allbritton, the chair of biomedical engineering focused on use of a
unique micropallet technology to isolate muscle and other stem cells. This has become the
central thrust of David Detwiler’s PhD project. Another graduate student, Nick Dobes, is now
involved in these studies. Dr. Allbritton focused her R01 renewal in this area, using the dog as a
model for translational studies; however, the grant was not funded. Resubmission is planned.
(2) Genomic-phenotypic correlation in GRMD dogs to better characterize the basis for
phenotypic variation. A graduate student, Peter Nghiem, has made considerable progress on
these studies, with two papers nearing completion/submission. They will continue to collaborate
with Eric Hoffman at Children’s National Medical Center in DC. (3) Mechanisms contributing to
muscle hypertrophy in GRMD dogs. They plan to submit a R01 for these studies this fall.
Because of ongoing concerns about the animal facilities, Dr. Kornegay has accepted a faculty
position at Texas A&M and will be transitioning this fall. The canine colony will probably be
moved to A&M in January 2012.
RUTH A. LININGER, M.D.
Dr. Lininger is a surgical pathologist who is a specialist in gynecologic and breast pathology.
She enjoys teaching residents, fellows, medical students, and graduate students and sharing what
25
she has learned. She works with medical colleagues in multidisciplinary conferences to help to
identify the best treatment and care for patients. Her research interests have become more
clinical during her career. She participates in collaborative studies, primarily in gynecologic
research on molecular markers in endometrial cancer and infertility states. She also has
entrepreneurial interests and has a growing private outside consult service focusing in
gynecologic and breast pathology and is a consultant for difficult gynecologic and breast
pathology cases for two regional reference laboratories. She enjoys helping to bring new
diagnostic tests to the special procedures laboratory, and also enjoys the business and fiscal
aspects inherent of running a surgical pathology laboratory where she provides input on billing,
charging, and other fiscal issues.
SUSAN T. LORD, Ph.D.
Dr. Lord’s research is focused on the coagulation protein fibrinogen. Their studies aim to provide
insight into the molecular mechanisms that mediate fibrinogen’s functions with the long term
goal of providing basic information relevant to the prevention, diagnosis or treatment of disease.
Their current studies examine the mechanisms that control fibrin clot formation. They have
examined an interesting new variant fibrinogen which is a hybrid between the human protein and
chicken fibrinogen. The hybrid fibrinogen is the first to show normal assembly during the first
phase of fibrin clot formation, but nearly complete absence of the second stage. They have
submitted a manuscript describing these findings. They have developed new techniques to
address this question, including dynamic light scattering. These experiments have been
productive. These data were presented at the ASH meeting and the ISTH meeting held in July
2011. They also continued to examine the role of FXIII in controlling clot formation. They have
developed the assays and reagents to monitor the kinetics and thermodynamics of these
reactions. These data were also presented at the ISTH meeting in July 2011. They are currently
writing manuscripts on both these projects.
Their studies on the strength and elasticity of fibrin clots and fibrin fibers continue to be
productive. Michael Falvo, in the Department of Physics at UNC-Chapel Hill, and Dr. Lord
have received NSF support for 3 years starting in August 2010. In these studies they measure the
forces needed to stretch and break individual fibrin fibers and small networks. Their studies
have shown that the remarkable mechanical properties of fibrin likely stem from an unfolded
region of fibrinogen, called the C region. These experiments were described in the publication
in the Biophysical J. They have synthesized variant fibrinogens to test the hypothesis that
changes in specific segments within this region will lead to changes in the elasticity and
extensibility of fibrin fibers. Dr. Falvo, Dr. Gorkun, and Dr. Lord published a review article on
this subject in Biophysical Chemistry. They submitted a multi-PI proposal to NIH in Feb. 2011.
CHRISTOPHER P. MACK, Ph.D.
The overall goal of the Dr. Mack’s lab is to identify the signaling pathways and transcription
mechanisms that regulate smooth muscle cell (SMC) differentiation. They have recently shown
that nuclear localization of the myocardin family SRF co-factors by RhoA signaling is an
important mechanism by which extrinsic factors regulate SMC-specific transcription. Their
current studies are focused on identifying the signaling pathways upstream and downstream of
26
RhoA that regulate SMC transcription with a particular focus on the role of this pathway in the
nucleus. The Mack lab recently identified the histone demethylase, jmjd1a, as an MRTF-A
interacting protein, and a relatively new and exciting research focus is on the epigenetic control
of SMC-specific transcription. The identification of the chromatin modifications that regulate
SMC-specific transcription is a major goal as is the identification of the chromatin modifying
factors that mediate these effects. They hope that their in vitro and in vivo studies will lead to
therapeutic targets for several cardiovascular pathologies that involve altered SMC phenotype.
NOBUYO MAEDA, Ph.D.
Apolipoprotein E plays a central role in lipoprotein metabolism and is required for the efficient
receptor-mediated clearance from plasma of chylomicron remnants and VLDL-remnants by the
liver. ApoE deficient mice as well as mice expressing human apoE isoforms instead of mouse
apoE have provided them tools to develop a deep understanding of the genetic factors underlying
atherosclerosis for some years. The apoE-/- mice in two inbred strains (C57BL and 129) develop
atherosclerotic plaques at different rates at different locations of their aorta. There are also
differences in geometric parameters of aorta between these strains. To test a hypothesis that
genetic factors determining aortic geometry determines susceptibility to plaque development,
they have been conducting Quantitative Trait Loci analyses of F2 mice between 129apoE-/- and
B6apoE-/- mice and between 129apoE-/- and DBAapoE-/- mice. The second project is to
determine the mechanism by which different apoE isoforms in humans influence lipid
metabolism, metabolic syndrome and cardiovascular incidents. They have recently found that the
mice with human apoE4 isoform are resistant to high fat-induced obesity compared to mice with
apoE3. However, adipose tissues in mice with apoE4 show reduced functionality, and mice
develop diet-induced insulin resistance earlier than apoE3 mice. Moreover effect of
rosiglitazone, an insulin sensitizer, on adipocyte differentiation is blunted in mice with apoE4
compared to mice with apoE3. They are currently investigating the isoforms-specific interaction
of apoE with PPAR during adipocyte differentiation and how it impacts to the susceptibility to
atherosclerosis.
NADIA A. MALOUF, M.D.
Dr. Nadia Malouf is ending her academic career with what she believes may be an important
contribution to the understanding of human adult-derived stem cell to transit from a “stemness”
state to a differentiated state. She found that a Ca2+ dependent calmodulin binding transcription
activator, CAMTA1, to be critical for the early commitment of adult-derived stem cells to a
myocardial lineage. She furthermore found that the role of Ca2+ signaling evidenced by
activation of the Ca2+dependent –Calcineurin-NFAT-RCAN1 pathway is involved in the same
process. The latter has recently been reported to be critical for the exit of ES cells from the stem
cell state. As CAMTA1 is a very recently recognized transcription factor in mammalian cells, its
role in ES cell differentiation has not been examined. Dr. Malouf’s research lead her to report
that Ca2+ signals in stem cells cause up-regulation in the expression of CAMTA1 and activation
of the NFAT pathway. Together these transcription factors trigger commitment of adult-derived
stem cells to a myocardial lineage. As it is difficult to discriminate between exit from stemness
and phenotype commitment she hypothesizes that CAMTA1 like NFAT may have an important
role ushering a stem cell out of “stemness”.
27
SUSAN J. MAYGARDEN, M.D.
Susan Maygarden, M.D., continues to be involved in clinical and translational research in
cytopathology and prostate pathology. She works with scientists and clinicians at Roswell Park
Cancer Institute, Buffalo, NY in submitting a prostate SPORE grant that will be collaborative
with UNC, Roswell Park, and Louisiana State University. Her role is working with tissue
procurement at UNC in collecting prostate samples, and assisting in grading and scoring tumors.
In clinical research, she is interested in the role of cytopathology in classifying tumors and
adding to diagnostic yield of operative cases. She has completed studies with Dr. Scanga on
classifying renal tumors by cytology, with Dr. Greene on classifying lung tumors by cytology,
and with Dr. Pierce and Dr. Veermachaneni on immediate cytologic interpretation of needle
biopsies and bronchial brushings during endobronchial ultrasound guided procedures.
CHRISTOPHER R. McCUDDEN, Ph.D.
Although Dr. McCudden will be leaving UNC in June 2011, there is substantial research work to
write up from his years in the Department. The four abstracts will all be submitted as
manuscripts in the short term, two have already been drafted (B2-transferrrin and V8 Capillarys).
He also intends to maintain collaborations with colleagues in the Department regarding serum
protein electrophoresis projects and development of new biomarkers for cachexia.
GAYLE C. McGHEE
Gayle McGhee works closely with the Pathologists to assist with any needed requirements
toward teaching responsibility. This responsibility includes working with the Director of Medical
Student Teaching in the Department to provide materials or computer support for lectures and
labs. Also, she is responsible for providing gross organs and helps schedule laboratory coverage
for labs and gives support to them in preparation for lab or lecture. She provides help for the
Director in preparation of quizzes and exams to be placed in Sakai for testing purposes. She
continues to work closely with autopsy personnel to maintain and gain additional teaching
material for the departments needs. Changes in autopsy volume continue to change so it is
important that the autopsy personnel and Ms. McGhee work more effectively together. All gross
specimens that are saved for teaching are collected and cataloged, inventoried, filed
appropriately, preserved and accessible by log system or computer search. The scanning of
virtual microscopy is now a vital part in teaching. She continues to scan slides and collect more
interesting slide cases for use in teaching. They have made their virtual images available to all
faculty by placing in a spreadsheet with diagnosis and important information as to retrieval of the
virtual images. The volume has increased this year with more scanning for research projects.
C. RYAN MILLER, M.D., Ph.D.
Dr. Miller’s current activities are focused on translational research involving comparative
genomics analysis of glioblastomas (GBM) from both humans and genetically-engineered mice
(GEM). The main goals of this work are to 1) develop a protein-level molecular classification of
human GBM with distinct response to the current standard-of-care therapy (temozolomide +
28
radiation (TMZ-XRT)); 2) define the impact of engineered genetic alterations and secondary
genetic events on astrocytoma subtype-specification in GEM; and 3) determine molecular
signatures of GEM GBM after TMZ-XRT.
The Translational Pathology Laboratory (TPL) continues to grow, both in terms of services
offered and the volume of services provided to UNC investigators. They have acquired several
new pieces of equipment and analysis software packages, including a CRi/Caliper Life Sciences
Nuance multi-spectral imaging camera and associated inForm analysis software, the Definiens
XD Developer and Tissue Studio image analysis software, and upgraded Aperio Spectrum image
and data management software available at https://tpl-spectrum.med.unc.edu. In calendar year
2010, they provided services to 63 investigators (up from 52 in 2009) and 18 clinical trials.
Diagnostic slides and FFPE tissue blocks were pulled from the UNCH Surgical Pathology
archives on 939 patient cases. Over 13,000 unstained tissue sections, 2,400 H&E stained slides,
and 4,400 IHC/IF stained slides were prepared. Over 15,000 slides were scanned on the Aperio
ScanScope XT and ScanScope FL systems and over 3,700 quantitative slide analyses have been
performed. Their services have been included in over 20 grant applications and 12 peer-
reviewed publications.
MELISSA B. MILLER, Ph.D.
Dr. Melissa Miller’s major interests reside in the use of molecular technology to improve clinical
infectious disease testing and, further, to use these technologies to explore the epidemiology of
viral infections and antimicrobial resistance in bacterial infections. During the past year, Dr.
Miller and colleagues completed and published a multi-year study of the prevalence and risk
factors of MRSA carriage in child care centers in North Carolina and Virginia. In addition to the
continued study of the molecular epidemiology of community-associated MRSA, she has begun
researching the prevalence of heteroresistant vancomycin-intermediate S. aureus (hVISA) using
clinical isolates. In collaboration with colleagues in the School of Pharmacy, she has initiated
risk factor analyses and outcome studies for patients identified as having an infection due to
hVISA. Dr. Miller’s laboratory serves as the core laboratory for the molecular characterization
of MRSA isolated from cystic fibrosis patients in two collaborative multi-center studies with Dr.
Muhlebach in the Department of Pediatrics. In addition, she is also is employing and comparing
a variety of molecular technologies, including microarrays and sequencing technologies, in the
clinical diagnosis and epidemiology of respiratory viral infections, the molecular diagnosis of
sepsis, and the detection of drug resistant Mycobacterium tuberculosis.
VINCENT J. MOYLAN, M.S., P.A. (ASCP)
Mr. Moylan is currently involved in three research activities. He is a co-investigator in a recently
funded NCTracs research grant entitled Characterization of Brain White Matter Development
using High Resolution Diffusion Tensor Imaging with Histologic Confirmation. He will be
assisting Drs. Joe Kornegay, Hongyu An, and Diane Armao. The second project is the LCCC
Tumor Donation Program. This is a rapid autopsy program headed up by Drs. Lisa Carey and
Leigh Thorne. This research program involves breast cancer patients that have previously
consented to autopsy upon their death. The third and final project is the CIMA (Comprehensive
Individual Molecular Atlas) project that is being coordinated through the Carolina Center for
29
Genomic Sciences. This project involves harvesting and dissection of all human body organs
from a previously consented donor. Additionally, he is working with Dr. Howard Reisner on
developing a searchable autopsy digital image database for use by appropriate departmental staff.
Also, he continues to work closely with Dr. Nickeleit and the Nephropathology department
handling all of the medical kidney specimens, and assisting the surgical PA’s by processing and
photographing select explant cases (cardiac, hepatic, lungs). He looks forward to continuing
work with Drs. Hadler and Aylsworth and other medical student related teaching projects as they
become available.
VOLKER R. NICKELEIT, M.D.
The research activities of Dr. Nickeleit focus on different aspects of renal allograft pathology. 1)
Adjunct markers (in particular tubular MHC-class II expression and capillary C4d deposition) for
the diagnosis of cellular and humoral graft rejection episodes in kidney and liver grafts are under
investigation. 2) A major research effort addresses polyomavirus infections in kidney allograft
recipients. A new and exciting line of investigation focuses on non-invasive diagnostic strategies
to establish a diagnosis of “polyomavirus nephropathy” without an (invasive) biopsy (in close
cooperation with H. K. Singh, MD). Negative staining electron microscopy on voided urine
samples and the search for three-dimensional polyomavirus clusters, “Haufen”, have proven in
pilot analyses to be highly robust diagnostic methods with negative and positive predictive
values of greater than 90%. Extended prospective studies are currently conducted in order to
validate the initial findings further. Additional in vitro and in vivo (mouse) studies are under way
or in the planning stage to elucidate mechanisms underlying the formation of Haufen and to find
alternative, diagnostically relevant ways of “Haufen” detection.
JUDITH N. NIELSEN, D.V.M.
Dr. Nielsen’s collaboration with Dr. Nancy Raab-Traub from the Lineberger Cancer Center in
her studies of Epstein Barr Virus LMP influence on tumor formation in mice has resulted in a
second paper submitted for publication describing the work demonstrating that the Epstein-Barr
virus LMMP1 and LMP2A function cooperatively to promote carcinoma development in her
mouse carcinogenesis model. She has also continued collaboration with Dr. Kirsten Nielsen, a
faculty member in the Department of Microbiology, School of Medicine at the University of
Minnesota, who is studying pathogenesis of Cryptococcus neoformans in a mouse model. This
collaboration has resulted in the funding of an R01 grant in which she will serve as a
collaborator. In addition, a three-way collaboration with Dr. Beverly Koller at UNC has begun,
using Dr. Koller’s knock-out mice to identify key steps in the establishment and progression of
cryptococcal infection. It is hoped that preliminary studies will result in preparation and
submission of further R01 grants. She has recently collaborated with Dr. Sha Chang in
preparation of an R01 grant to validate development of new imaging and treatment modalities for
cancer, using the pig model to test and validate new state-of-the-art CT imaging equipment.
Dr. Xiaochen Lu, a Research Specialist under the supervision of Dr. Craig Fletcher and Dr.
Nielsen have completed setting up the DLAM Molecular Diagnostic Testing Laboratory and they
are now beginning to use his assays to track outbreaks of mouse parvovirus, mouse hepatitis
virus, helicobacter and pinworms in our mouse colonies at UNC. It is hoped that they will find
30
this more efficient and cost effective than use of referral laboratories. These facilities can also be
used for research activities by their faculty.
SIOBHAN O’CONNOR, M.D.
Dr. Siobhan O’Connor is currently reviewing cases for project LCCC 9830, for which over 800
new breast cancer cases have been collected. Tumor is being cored from paraffin-embedded
tissue to be used for molecular studies and tissue microarrays. As part of this study, genetic
variability in breast cancer is being investigated in relation to how it affects response to various
chemotherapy regimens. This tissue will also be available for Dr. O’Connor to develop her own
projects. Also, she is expecting to receive 20% salary support from the breast SPORE and will
work on additional projects in collaboration with the oncologists and Dr. Charles Perou. Dr.
O’Connor is working with Dr. John Woosley on educational videos for the AAMC, which will
result in publications, the first of which is on acute chorioamnionitis.
KUMAR PANDYA, Ph.D.
Dr. Pandya’s primary interest lies in understanding the molecular networks underlying complex
genetic switches in vivo in response to development and pathological stimulations. During his
postdoctoral tenure, he has focused on investigating the cellular and molecular basis of heart failure,
with an emphasis on changes occurring at the level of individual myocytes. He generated multiple
gene-targeted mouse-models that express multiple fluorescent proteins as indicators of hypertrophy-
responsive genes. His work has demonstrated that multiple pathological features of cardiac
hypertrophy such as the MHC switch, MEF2 activation, and altered beta-adrenergic responsiveness
occur in discrete subsets of, rather than uniformly in all, myocytes. His current work is focused on
uncovering the degree (both qualitative and quantitative) to which various pathological facets manifest
in individual cells, with the long term goal of identifying the in-vivo effectors and attendant
transcriptional pathways of pathological switches. His work is also focused on determining the
epigenomic landscapes in heart failure and investigating the roles of chromatin modifying factors
(brahma and brahma related gene1) in heart development and failure.
He attained his first faculty position in January, 2011, and since then he was awarded an internal
research grant from UNC. He has also submitted an R21, and plans to submit an R01 in fall 2011, and
an AHA research award in January, 2012.
YARA A. PARK, M.D.
Dr. Park’s research focuses on thrombotic thrombocytopenic purpura (TTP), specifically the
causes and exacerbating factors. Currently, she is investigating the role of infection in both the
initial presentation of TTP as well as exacerbations during treatment. She is also looking into the
role of B cell activating factor in the pathogenesis of TTP with Stefanie Sarantopolous and Yuri
Fedoriw. Additionally, she is the co-PI for the UNC site of the Transfusion Medicine and
Hemostasis Clinical Trials Network grant.
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KATHLEEN W. RAO, Ph.D.
In April 2011, Dr. Kathleen Rao was elected Chair of the Children’s Oncology Group
Cytogenetics Committee – term to begin July 1, 2011. Over 100 Cytogenetics laboratories in the
US and foreign countries participate in COG Cytogenetics studies. Non-US labs include
laboratories in Canada, Australia, New Zealand, Ireland, and Switzerland. The Children’s
Oncology Group (COG) is the world’s largest, cooperative children’s cancer research group.
HOWARD M. REISNER, Ph.D.
Dr. Reisner enjoys teaching and the preparation of course related material. The ability to design
and execute a course on one’s own (such as the Dental General Pathology and the Undergraduate
Mechanisms of Disease Class) allows for creativity, some degree of authority along with the
responsibility. One is likely to deserve the student comments one receives (and his continue to
be been quite good). His work with the Aperio Image Analysis platform has led to collaboration
in a project with Dr. Nancy Thomas, Drs. Singh and Nickeleit and others. He has been involved
in a research project with one of Dr. Tidwell’s former graduate students (Rachel Goldsmith)
which led to a number of publications and will continue to undertake projects involving new
educational materials in collaboration with Dr. Woosley. The most recent involves adapting an
Ajax/Seadragon browser for use with pathology images. Dr. Reisner plans to complete two
pathology textbooks during the coming year.
ARLIN B. ROGERS, D.V.M., Ph.D.
Dr. Rogers’ lab is focused in host/environment interactions in chronic liver disease (especially
cancer), with a special interest in the role of gender on disease progression. They are
concentrating their current efforts on the impact of cell signaling and epigenetic remodeling with
a special emphasis on STAT5 in hepatocarcinogenesis using mouse models and cell culture
systems. These studies may reveal the molecular mechanisms accounting for marked gender
disparity in human HCC, and will suggest new biomarkers and interventional targets. Goals for
the coming year include procuring an R01 and initiating animal studies to complement their
investigation of STAT5 and inflammatory signaling in hepatocyte cultures. In addition to his
primary research activities, Dr. Rogers collaborates widely as a veterinary pathologist and co-
investigator within DPLM and SOM on experiments using mouse models to study chronic
inflammatory disease and/or cancer.
LORI R. SCANGA, M.D., Ph.D.
Dr. Scanga’s current clinical service activities include anatomic pathology signout in the areas of
both surgical and cytopathology. She has signed out over three thousand anatomic pathology
cases since she began clinical service as faculty including surgical pathology, outside surgical
pathology consults, gynecologic cytology, fine needle aspirations, exfoliative cytology, and
outside cytology consults. Also, she has covered frozen sections and surgical pathology call.
She started clinical service in the areas of cytology and gynecological surgical pathology, and
has since added service on the ENT surgical pathology bench. Her clinical service on the ENT
bench will surpass her clinical service weeks on gynecologic pathology in the next six months. In
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the first half of the 2011-2012 academic year, she will be on ENT service for six weeks,
gynecologic pathology for five weeks (both benign and oncologic gynecology services), and
cytology for six weeks. The surgical pathology areas of both ENT and gynecologic pathology
are complementary to her cytology service, and plan to continue with signing out all four of
these service benches. Her research goals include publishing her work studying kidney fine
needle aspirations which she recently published as an abstract in Cancer Cytopathology entitled
“Utility of Fine Needle Aspiration and Core Biopsy with Touch Prep in the Diagnosis of Renal
Lesions” and presented as a poster at a national meeting of the American Society of
Cytopathology 58th
Annual Scientific Meeting in Boston, November 12-16, 2010. She received
IRB approval of this project and she is writing a manuscript to publish this work in an
anatomical pathology journal. She enjoyed teaching medical students in the MS2 Reproductive
Medicine Block and will continue to teach in this block. She enjoys teaching around the scope
sessions to residents and will continue to teach residents in formal teaching lectures and at the
microscope during sign out.
JOHN L. SCHMITZ, Ph.D.
Dr. Schmitz’s current and upcoming research activities are focused in the areas of HIV
Immunology and Transplant Immunology. With the successful re-competition of the UNC
CFAR Dr. Schmitz’ CFAR Immunology Core will be funded for 5 years as part of this effort
(2011 – 2016). In addition to the support provided to HIV researchers at UNC and Duke
University, Dr. Schmitz has been awarded 3 contracts with the National Marrow Donor Program
to provide laboratory infrastructure and testing services for clinical trials of bone marrow
transplant in HIV patients. In addition, Dr. Schmitz is conducting 2 vendor sponsored clinical
evaluations of HIV testing method including a rapid diagnostic test and a point of care CD4 test.
In the upcoming year, Dr. Schmitz will have 3 additional contracts with Becton Dickenson to
support HIV related clinical studies of a CD4 testing method and to conduct a pediatric CD4
normal range study in collaboration with Dr. Steiner from Pediatrics. In the context of transplant
immunology Dr. Schmitz continues to collaborate with clinical transplant colleagues on defining
the role of HLA antibody in liver transplant recipients. In addition, Dr. Schmitz’s laboratory is
participating in a multicenter study describing the natural history of alloantibody production
post-transplant in solid organ recipients. This work is being performed as a study for the 2012
International Histocompatibility Workshop. Dr. Schmitz is also planning to collect peripheral
blood lymphocytes from these same patients to assess B cell phenotypes and function with the
goal of applying for TRACS funding to study the regulation of B cell responses in allotransplant
recipients. This work will be done in collaboration with UNC investigators studying the role of
B cell responses in graft versus host disease.
DENNIS A. SIMPSON, Ph.D.
Dr. Simpson’s research, in collaboration with Dr. Kaufmann, is currently focused on
understanding how normal human melanocytes respond to exposure to ultraviolet light and how
this response protects the cells from changes that result in melanoma. To do this he has
developed a novel system to measure the viability of melanocytes after UV exposure and he has
adapted the Tet regulation system for use in melanocytes to allow the controlled expression of
important oncogenes such as mutant B-Raf. Over the next year he hopes to get a measure of the
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UV induced mutation frequency of melanocytes and a determination as to how protective
melanin is to a melanocyte as opposed to a keratinocyte.
HARSHARAN K. SINGH, M. D.
Dr. Singh will continue to work further in the characterization and development of a novel, non-
invasive, diagnostic test to diagnose a major infectious complication post kidney transplantation
known as polyomavirus nephropathy. This new diagnostic technique developed by Dr. Singh and
her colleagues at UNC avoids invasive biopsy procedures, and could potentially have profound
implications for the care of kidney allograft recipients worldwide. Currently, this test requires the
use of electron microsocopy. New methods including ELISA assays and flow imaging
techniques are being evaluated to eliminate the necessity of using electron microscopy for test
performance. The clinical impact of this novel discovery will continue to be confirmed in a
multi-center prospective study with unrestricted funding from Astellas Pharma, US Inc. for
which UNC Nephropathology is the lead investigative center. The Division of Nephropathology
is also the lead investigator with centers participating from the US, Canada, and Europe in
developing an International Consensus Classification of Polyomavirus nephropathy. Initial
results from this work will be presented at the International Banff Meeting, Paris, France, in June
2011.
SCOTT V. SMITH, M.D.
Dr. Smith’s current clinical activities are focused in surgical pathology with broad emphasis in
pediatric, ENT, thoracic, genitourinary, prostate, cardiovascular, pancreaticobiliary, endocrine,
bone, and soft tissue pathology. Dr. Smith’s research interests are clinical translational studies in
solid pediatric tumors. He has undertaken collaborations with Dr. Julie Blatt and Dr. Ian Davis
in the Division of Pediatric Hematology Oncology in the Department of Pediatrics. He is
working with Dr. Ian Davis in Pediatric Oncology on genome-wide identification of active
regulatory elements in fresh and archival human cancers.
OLIVER SMITHIES, Dr. PHIL
Dr. Smithies’ research has been working towards understanding genetic factors that influence
hypertension and kidney damage in diabetics - conditions with strong genetic and environmental
components. Currently, they have shown that genetic changes which affect the level of
expression of the genes coding for angiotensinogen (AGT), or for renin, or the type 1a receptor
for angiotensin II (Atr1a), or the endothelial form of nitric oxide synthase (eNOS), or the atrial
natriuretic factor (ANF) or two of its receptors (NPRA and NPRC), all affect blood pressures in
the mouse. Surprisingly, comparable changes in the gene coding for the angiotensin converting
enzyme (ACE) do not alter blood pressures. This finding led them to their most recent work
which uses animal models to understand the genetic basis for differences in the risk of kidney
damage in diabetic individuals. Additionally, Dr. Smithies is using gold nanoparticles to test his
hypothesis that the glomerular basement membrane is the place where the kidney separates
differently sized macromolecules.
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JOAN M. TAYLOR, Ph.D.
The long-term goal of Dr. Taylor’s research is to identify signaling mechanisms that contribute
to normal and pathophysiological cell growth in muscle (smooth, cardiac, and skeletal). She is
interested in studying cardiac and vascular development as well as mechanisms involved in heart
failure, atherosclerosis, and muscle degenerative diseases. The current directions of the Taylor
lab are to characterize components of the integrin signaling cascade in these specialized cell
types and to target disruption of these regulatory molecules in vivo in an effort to determine their
precise role in cardiovascular growth and development. Plans for 2011/2012 are listed below
Cardiac: One of their lines exhibits sudden death after pressure overload that is associated with
the redistribution of FAK to cell-cell junctions. They are using a proteomics approach coupled
with telemetry to uncover the mechanisms by which alterations in integrin signaling may
promote arrhythmia.
Skeletal: They have uncovered a novel pathway that is regulated by beta dystroglycan and likely
plays a critical role in the pathogenesis of muscular dystrophy. They are exploring new concepts
with respect to how muscle cells differentiate and fuse in the hopes of creating new compounds
that will be effective in blocking muscle wasting that occurs in a multitude of diseases.
Smooth: They are particularly interested in defining the mechanisms that regulate the induction
and recruitment of smooth muscle cells from the epicardium to the coronary vasculature. They
have data that implicate LIM domain containing proteins in this process and will continue to
develop animal models to precisely define their role(s) in this important developmental process.
LEIGH B. THORNE, M.D.
Dr. Thorne’s clinical duties currently include attending for the Molecular Genetics Lab and the
Autopsy service as well as Director of Autopsy Services. Translational research activities
continue with her involvement in the LCCC Tissue Procurement Facility. They continue working
towards development of a universal/global consent in conjunction with the UNC Health Registry
with the goal of consenting all potential cancer patients who visit the NC Cancer Hospital. New
in the upcoming year is the revival of the breast cancer rapid autopsy program. She hopes that
this will be successful and eventually expand to include other tumor types. Dr. Thorne will also
continue to focus on raising the standards of the LCCC TPF by bringing the facility into
compliance with the NCI Best Practices Guidelines as they expand to include smaller institutions
in North Carolina.
RICHARD R. TIDWELL, Ph.D.
Dr. Tidwell will continue the collaboration with the Genomics Institute of Novartis Research
Foundation (GNF). This collaboration has allowed the Tidwell led Consortium for Parasitic Drug
Development (CPDD) to access to a library of over 300,000 small molecules to screen and
optimize for development as treatments for late stage human African trypanosomiasis (HAT).
This developmental program is being funded under the current Gates Foundation Grant and
supplemented by GNF scientist and facilities. In addition, work continues on another Gates
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funded grant to determine mechanism of toxicity for new drug candidates to treat HAT. This
grant is a collaborative study with the Hamner Institute for Drug Safely. This grant’s ultimate
goal is to uncover specific markers to predict renal and liver toxicity of new classes of
molecules. An R01 grant proposal entitled “Novel Approaches to Ensure Safety of Promising
New Drugs for Sleeping Sickness” was submitted to NIH during the past year. Although the
grant received mixed reviews ranging from all ones by one review to twos and threes by another
review it was not funded. This proposal is being rewritten in response to reviewers’ comments
and will be resubmitted in 2012. At the invitation of the NIH, Dr. Tidwell submitted a P01
proposal entitled “Development of New Molecular Scaffolds to Treat Stage 2 Sleeping Sickness”
in the spring of 2011. This proposal is in collaboration with the University of Washington with a
requested direct cost budget of $3,657,354 spanning a five year period. A joint venture is current
being negotiated between the University of North Carolina (for the CPDD) and Developing
World Health (DWH). Once this JV is completed with this Scottish based non-profit, DWH will
assume the role of raising money for the CPDD. Finally, they continue their collaboration with
Bayer Animal Health to jointly research new drugs to treat animal diseases.
MICHAEL D. TOPAL, Ph.D.
Michael Topal’s current research involves 1) helping The Cancer Genome Atlas project in its
goal to characterize gene expression in many types of tumor samples, and 2) bioinformatics
analysis of gene organization in human viruses. The rest of his time is spent on administrative
initiatives associated with basic and translational science core facilities at UNC, as described in
Section E, and as Faculty Director of the Genomics and Mammalian Genotyping core facilities.
The coming year will see discussions to build a regional genomics institute concentrating on high
throughput sequencing, to reform HR policies concerning core directors and staff, to centralize
core facilities on campus and to provide centralized management of the facilities, and to build a
web portal that will be transformative in its ability to educate clinical researchers about research
infrastructure applicable to clinical samples.
DIMITRI G. TREMBATH, M.D., Ph.D.
Dr. Trembath is currently investigating novel markers for the diagnosis and prognosis of brain
tumors, in collaboration with Dr. Ryan Miller. Dr. Trembath also wants to develop
collaborations with researchers in the neurosciences investigating animal models of
developmental neurologic disorders such as autism.
CYRUS VAZIRI, Ph.D.
Dr. Vaziri’s major goal is to publish results of ongoing research projects in high quality journals
in order to maintain existing grants and to provide additional funding opportunities. Another
goal is to identify novel areas for future research and to initiate new projects that will provide
vehicles for extramural funding. To this end, he has initiated trans-disciplinary studies with
several colleagues at UNC including Dr. Bill Janzen (School of Pharmacy), Dr. Jim Swenberg
(CEHS), and Dr. Monte Willis (Pathology). The collaborative drug discovery project with Dr.
Janzen has resulted in an award from the UCRF ($187K) and a graduate student working on this
project (Alicia Greenwalt) has procured support from the Program in Translational Medicine.
36
Two grant applications to support the collaborative projects with the Swenberg lab have been
submitted to the NIEHS and a collaborative proposal with Dr. Willis for submission to the NIH
is in preparation.
TRACIE WAGNER, B.S., P.A.
Ms. Wagner is primarily responsible for triaging and banking specimens for the Tissue
Procurement Facility. She has increased the amount of specimens banked from about 20% to 60-
80%. Her goal is to have 95-98% of the cases consented banked. She has also implemented the
banking of prostate cancer from prostatectomy specimens, which before she came, were not
getting banked at all. She has become the clinical instructor of the Frozen Section Room. She
has standardized the work flow and implemented the lean concept. She is now the sole instructor
responsible for training all first year residents as well as assisting/training 2nd
-4th
year residents
and fellows in the frozen section room.
KAREN E. WECK, M.D.
Dr. Weck’s recent research efforts are focused on developing pharmacogenetic testing to predict
response to drug therapy. Her laboratory is collaborating in several clinical trials at UNC to
study the clinical utility of pharmacogenomic guided therapy. One ongoing clinical trial is a
prospective randomized study to determine the utility of pharmacogenomic guided dosing of
warfarin, incorporating genotyping for variants in the VKORC1 and CYP2C9 genes associated
with altered warfarin response. Dr. Weck is a member of the International Warfarin
Pharmacogenomics Consortium, whose goals are to study the effect of clinical and genetic
factors on warfarin response and to devise a pharmacogenomic dosing algorithm for warfarin.
This work has resulted in two recent publications. Plans are underway to further analyze
genomic and clinical factors associated with warfarin response in different ethnic populations
including African Americans. In addition, Dr. Weck has collaborated with a group in Brazil to
identify VKORC1 mutations associated with warfarin resistance. Dr. Weck is also a co-
investigator in a multicenter collaborative clinical trial to study the efficacy of CYP2D6
genotype-guided dosing for tamoxifen in breast cancer that includes UNC and several other sites
across North Carolina. The preliminary results of this trial indicated that CYP2D6 genotype-
guided dosing of tamoxifen resulted in normalization of plasma concentration of endoxifen, the
active metabolite of tamoxifen, in women who are CYP2D6 intermediate metabolizers. The
tamoxifen trial has been expanded to include 500 women across North Carolina, with escalated
recruitment of African American and Hispanic women. Dr. Weck is also collaborating with
investigators in the Departments of Cardiology to conduct a clinical trial on the efficacy of
CYP2C19 genotype-guided dosing for clopidogrel.
Another major effort is translation of new knowledge of the genetic causes of disease into
diagnostic testing. Dr. Weck’s laboratory has developed mutation testing for genes associated
with primary ciliary dyskinesia, X-linked Alport syndrome, and focal segmental
glomerulosclerosis (FSGS). The goal is to better characterize the spectrum, incidence and
genotype-phenotype correlation of mutations associated with disease and to develop clinical
testing in those genes with clinical utility. The UNC Molecular Genetics Laboratory is now one
of the only laboratories in the country that offers clinical genetic testing for mutations associated
37
with these diseases. Finally, efforts are underway to incorporate whole exome sequencing
technology for clinical diagnosis. Dr. Weck collaborated on a study to evaluate the accuracy of
massively parallel sequencing for detection of genetic variants associated with primary ciliary
dyskinesia, published recently in the journal Genetics in Medicine. She is co-investigator on two
new NIH grant submissions to continue this work.
BERNARD E. WEISSMAN, Ph.D.
Dr. Weissman's current research focuses upon the role of aberrant chromatin remodeling in
cancer development. Specifically, his laboratory concentrates upon loss of activity of the
SWI/SNF chromatin remodeling complex in the development of non-small lung carcinoma, a
cancer strongly associated with environment pollution, small particle exposure and smoking and
malignant rhabdoid tumor, a rare pediatric cancer. Previous studies from Dr. Weissman's
laboratory have shown that inactivation of individual components of the complex alter gene
expression through changes in chromatin organization. Furthermore, the loss of SWI/SNF
complex may induce epigenetic instability in cancer cells leading to gene silencing via a
mechanism independent of DNA methylation. Current studies focus upon understanding how
loss of SWI/SNF complex remodeling activity alters signaling of major signaling pathways
associated with cancer development including the WNT, NFkB, and KEAP/NRF2 pathways.
The laboratory employs cell culture and genetically engineered mouse models combined with the
latest molecular genetic techniques including ChIP-seq and MNase-seq to address these
questions.
HERBERT C. WHINNA, M.D., Ph.D.
Dr. Whinna’s research interest is in how the normal process of hemostasis occurs in time and
space to plug an injury site without causing pathologic thrombosis in the entire vasculature.
First, this involved basic protein chemistry studies on the structure-function aspects of thrombin
inhibition by the serine protease inhibitors antithrombin III and heparin cofactor II (PhD thesis
work). Next, he began engineering and testing of chimeric antithrombin molecules that combine
favorable properties of sometimes diverse naturally occurring molecules that can act when and
where it is most desirable (postdoctoral and early faculty work). As part of the testing of these
engineered antithrombins he established and refined thrombosis models in mice and has been
using these models to study the effects of not only the molecules he has engineered, but also
(through collaborations) other naturally occurring and synthetic molecules. He has shown
previously unreported pathophysiologic differences in two of the most widely used models of
thrombosis in mice, which have important implications for studies utilizing these models and the
conclusions drawn from them. Most recently, he has developed improved murine hemostasis
models that can be correlated with know phenotype in human disease. These models are being
used to test the effects of both pro- and anti-coagulant compounds in order to delineate both
normal and pathologic hemostasis. Additionally, these models are being applied in mouse
models of human disease to investigate both pathophysiologic mechanism and possible
treatments.
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JULIA W. WHITAKER, M.S., D.V.M.
Dr. Whitaker will continue to provide veterinary clinical care for the research animals on campus
as her primary function. With the new animal facilities opened on campus, the mouse census
will more than double, which will significantly increase the case load. She will also continue to
pursue research on the effect of caging environment on mouse reproduction and behavior, in
collaboration with Dr. Sheryl Moy in the Department of Psychiatry, for which Dr. Whitaker has
submitted grant applications this year. Her interest and specialty training in aquatic animal
medicine will continue to be used to support the aquatic research species on campus. She will
also continue to be involved in teaching and training of laboratory animal residents in the
Research Triangle area through the new Research Triangle Laboratory Animal Training Program
seminar, Pathology graduate students on animal models, and investigators and laboratory staff on
the use of animals in research. She will continue to co-chair the Southeastern location of the
International Mock Board Exam Coalition for the ACLAM board exam and to serve as Interim
Associate Director of Veterinary Services.
MONTE S. WILLIS, M.D., Ph.D.
The Willis laboratory investigates the role of the ubiquitin proteasome system in the
pathophysiology of cardiac hypertrophy and heart failure. Cardiac hypertrophy develops in
response to biomechanical stress most commonly from extrinsic pressures such as hypertension,
valvular heart disease, or myocardial infarction. The development of cardiac hypertrophy results
in metabolic and structural changes in cardiomyocytes. One metabolic change prominent during
adaptive cardiac hypertrophy involves a switch from utilization of fatty acids as an energy
source; while fatty acid oxidation is not detrimental to the healthy heart, during prolonged
periods of stress fatty acid intermediates contribute to free radical stress. Instead, the stressed
heart relies on glucose utilization, allowing reduced oxidative stress, a metabolic switch widely
believed to preserve cardiac function in cardiac hypertrophy and heart failure. Recently, the
peroxisome proliferator-activated receptor (PPAR) family of nuclear receptor transcription
factors has been shown to regulate the cardiac switch from fatty acid to glucose energy at the
level of gene transcription. As such, decreasing PPAR- levels in the setting of cardiac
hypertrophy reduces expression of “bad” or “lipotoxic” fatty acid oxidative enzymes while
simultaneously enhancing those responsible for “good” glucose oxidation. Identification of the
factors that regulate PPAR mediated metabolic adaptation during the development of cardiac
hypertrophy and heart failure would open up new therapeutic options for preserving heart
function. The Willis laboratory has recently identified that the cardiac ubiquitin ligase Muscle
Ring Finger-1 (MuRF1) is an essential regulator of the adaptive changes in metabolism that
accompany cardiac hypertrophy and heart failure. They have further identified that MuRF1
specifically interacts with and inhibits PPAR- activity, by changing its nuclear localization via
mono-ubiquitination and nuclear export, which is consistent with PPAR inhibition
accompanying adaptive cardiac hypertrophy. In the next year, the focus of the laboratory will be
on determining the how MuRF1 regulates nuclear export of nuclear receptors as well as how its
own expression is regulated transcriptionally through external forces. These studies will
establish the molecular mechanisms by which characteristic shifts in metabolism occur in
response to cardiac stress and lead to heart failure.
39
RUTH E. WINECKER, Ph.D.
The laboratory is currently researching the implications of post mortem redistribution of SSRI’s
in death investigations. There is one planned publication and one planned abstract from this
research for 2011-2012. The laboratory recently submitted an abstract on metaxalone involved
deaths for presentation at AAFS in Feb 2012. A manuscript of this research is currently being
prepared. Also, manuscripts for research already conducted and presented at scientific meetings
in 2010-2011 are currently in preparation. Topics include levetiracetem and buprenorphine.
ALISA S. WOLBERG, Ph.D.
The major goals of Alisa Wolberg, PhD, are to: 1) examine cellular, biochemical, and
biophysical features that modulate thrombin generation, and 2) determine how the pattern of
thrombin generation dictates clot formation, structure, and stability. Dr. Wolberg’s group has
made substantial progress towards both goals during this year. They have used in vitro assays
and developed novel in vivo models of thrombosis and thrombolysis to examine how plasma
hypercoagulability and vessel injury promotes thrombus formation. Their studies suggest
pathogenic roles for cell-derived microvesicles in clot formation, and correlate thrombus
formation and stability with extent of vessel injury. Their techniques for measuring fibrin
formation and stability may provide important information on the therapeutic dosing window of
novel thrombolytic and hemostatic agents.
JOHN T. WOOSLEY, M.D., Ph.D.
Dr. Woosley has continued and expanded his research in GI and Liver pathology. He is the study
pathologist in a large (~800 cases) population-based study of colon cancer in 33-counties of
North Carolina examining traditional dietary and lifestyle risk factors, access and utilization of
health services; and polymorphisms of carcinogen metabolizing enzymes. As a companion study,
they have enrolled some of these subjects in a national colorectal cancer family registry that will
have the potential to identify genetic markers for colon cancer risk. A major objective of this
study is to determine why African-Americans with colon cancer fare more poorly than Caucasian
Americans. A follow-up proposal dealing with the same issues for rectal cancer has also been
funded. In addition, Dr. Woosley continues his scholarly activity on the technology of pathology
education.
HONG XIAO, M.D.
Dr. Xiao’s major research goal, in collaboration with Dr. Jennette, is by using their innovative
mouse models of antineutrophil cytoplasmic autoantibody (ANCA) induced glomerulonephritis
(ANCA disease), to advance the understanding of mechanism of the ANCA mediated
autoimmune disease. Her current focus on studies: (1) testing involvement of Fcγ receptors and
alternative complement pathway in pathogenesis and therapeutic interventions in ANCA disease
mouse model, such as by blockage of Fcγ receptors or C5a receptors with specific antibodies or
small molecule inhibitors, which may have important implications for therapies of human
diseases; (2) inducing experimental ANCA disease models by neutrophil antigens in addition to
MPO protein, such as Proteinase-3 (PR-3) and lysosomal membrane protein-2( LAMP-2), or
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different portions of MPO for identifying specific epitopes that are targeted by pathogenic anti-
MPO antibodies; (3) investigating genetic basis for variations in severity of ANCA disease
among different strains of mice, which mimics disease variations in ANCA patients and trying to
identify candidate genes and their protein products responsible for the differences in disease
severity, which might be new markers for disease activity and potential targets for novel
therapeutic strategy in humans.
XIANWEN YI, M.D., Ph.D.
Dr. Yi’s studies in collaboration with Dr. Maeda, focused on antioxidant animal models for
effective treatment of diabetic nephropathy and inflammation. After successfully creating these
mouse models, putting them through initial tests and conducting other research in vitro, his
research is becoming fruitful. He published two articles within the last year and another
manuscript has been accepted. His work is gradually being recognized in the diabetic research
field. He has received five manuscript review requests from different journals and an invited talk
for a national meeting in the past few months. He successfully received an innovative grant from
American Juvenile Diabetes Research Foundation recently. He also submitted a R01 grant
application last month and currently, he is preparing another R01 application in a different
research project. He hopes he can make more contributions to research in their department in the
coming year.
MAIMOONA B. ZARIWALA, Ph.D.
Dr. Zariwala’s research is focused in several areas: (1) Test for new candidate genes for primary
ciliary dyskinesia, (2) To test new patients for known gene mutations, (3) Continue to expand the
CLIA approved clinical genetic test panel for Primary Ciliary Dyskinesia, (4) Provide
consultation and ongoing support to the Molecular Pathology Lab for clinical genetics test panel
for Primary Ciliary Dyskinesia, and (5) Decipher possible genetic causes of idiopathic
bronchiectasis that is not related to the CF or environmental causes. Dr. Zariwala’s laboratory
has made significant progress towards each of these goals in the last year. The work on
DNAH11 mutation profiling is complete and manuscript is under revision. Collaboration is
ongoing with Dr. Omran in Germany on the newly identified PCD-causing genes CCDC39,
CDC40 and previously known DNAH5 mutation profiling. The replication work on DNAH5 and
CCDC40 is near complete and additional mutations have been identified from our patient cohort.
Ongoing collaboration with the national and international laboratories through the Primary
Ciliary Dyskinesia consortia, additional patient material is acquired and tested for known genes
and mutations, as well as for defining novel mutations. Additionally, Dr. Zariwala is involved
with Drs. Evans, Weck and Berg to test the possible usage of the next generation sequencing
technology in the clinical setting and manuscript is already published. Through the collaboration
formed with Drs. Shendure, Nickerson and Bamshad at Seattle Genomic Sequencing Center,
work on Whole-Exome sequencing of 24 unrelated PCD patients is continued. Of these 24
samples, they identified a novel founder mutation in 3 families with Ashkenazi Jewish ethnicity
in the previously known DNAI2 gene. Additionally, they found mutations in the known DNAH5
gene in two families and RSPH9 in one family. Furthermore, Dr. Zariwala identified TWO
NOVEL PCD causing genes from this cohort. Mutation Profiling of novel genes identified
additional patients harboring mutations, thus these are indeed PCD-causing genes. Functional
41
analysis of splice mutations in the genes revealed that mutations were affecting splicing
machinery. Further work on novel genes is underway. Additionally, with the group in Seattle,
Dr. Zariwala carried out Whole-exome sequencing in 24 samples from 17 families with non-CF
bronchiectasis. Many of these patients have some overlap with PCD phenotype and/or idiopathic
bronchiectasis. The initial analysis revealed TWO NOVEL cilia related genes in two families
that had non-classic PCD phenotype. Large scale mutation profiling and further characterization
work is underway. Additionally, the data analysis and validation studies of whole-exome
sequencing for PCD as well as non-CF bronchiectasis group is ongoing in patients where genetic
causes are not yet identified. Moreover, Dr. Zariwala has formed collaboration with Dr.
Hildebrandt (University of Michigan) and Dr. Cecilia Lo (University of Pittsburg) to carry out
whole-exome sequencing in additional ~50 PCD families, and that work is underway. In a
nutshell, 4 novel cilia related genes have been identified in the past year and additional work is
ongoing to find new targets for PCD and non-CF bronchiectasis. The success of this test will
open the door for expanded clinical tests as early diagnosis will allow early intervention and will
improve clinical outcome of classic and non-classic PCD as well as idiopathic bronchiectasis.
This study will also represent a significant step forward in the application of new approaches to
genetically heterogeneous disorders in humans.
TEACHING
HOWARD M. REISNER, Ph.D.
MEDICAL:
Second Year Medical School Involvement: Pathology content provided by our department is
incorporated into 10 of the 11 blocks which comprise the second year curriculum. The blocks are
predominantly organ system based however two blocks, an introductory "Tools" block and a
Clinical Medicine Cases Block serve special functions to be discussed. The only organ system in
which the department does not play a strong role is the Musculoskeletal/Dermatology block
which supplies its own expertise. However, we support the block in providing virtual scanned
images for use. Each organ system block is represented by a member of this department serving
on a "block committee". Several committees are chaired by departmental faculty members
including the Tools Block. Each block attempts to integrate pathology and abnormal
physiology/medicine into a single course with a single syllabus (all presented on-line). Different
blocks have taken somewhat different approaches but, in general, independent pathology lectures
remain relatively intact and are usually broken into small units. The tendency for "independent"
pathology laboratory sessions to be used in several of the blocks (including respiratory, GI,
endocrine, female reproductive and renal/gu) has continued and receives excellent student
comments. These "mini-pathology" lab sessions are most successful when presented before the
more medical sections of the laboratory (when such exist) and are designed so as to complement
other material presented. The availability of laboratory staff that participate in multiple blocks
(particularly Dr. Hadler) allows students to get to know our faculty members across several
organ system blocks and student attendance in laboratories although somewhat reduced remains
acceptable. Several blocks incent attendance via a variety of grading schemes. In addition, an
introduction to Pathology as a medical career is now part of the Tools block. This allows an
additional venue for interested students to meet our faculty. In addition, several of our more
junior faculty have used this and laboratory/lecture participation as an opportunity to meet
students. Twelve video podcasts presenting overviews of introductory laboratory are available to
42
the first block and were noted as helpful by students. The availability of gross organ specimens
in the small group sessions continues to an extremely positive development in laboratory/small
group sessions and the department is pleased that such specimens were available for and heavily
used this academic year. The revised on-line web-based quiz system (MedStars) was used
pathology evaluations and was a tremendous improvement over the older system.
The Tools Block (Block 1) now includes the entire Introduction to Pathology (General
Pathology) sequence and is taught in conjunction with Radiology. We have added to additional
small group sessions (vascular and developmental pathology) and have integrated radiology
material in all of the “workshops”; a process which will be continued this year. An autopsy
experience run through the hard work of the Office of the Chief Medical Examiner and
coordinated by Dr. Reisner is now part of the Clinical Case Block.
Dr. Reisner has attempted to aid in preparation of teaching material with the assistance of Ms.
McGhee and they have concentrated on making virtual microscopy slides easily available as part
of the syllabi. All blocks used computer based virtual microscopy rather than glass slides and
microscopes to present histopathological material and the availability of a new Aperio scanner
with 40X capabilities has allowed the extension of VM technology to the area of
hematopathology. Images are provided online via a specialized image server which also serves as
the repository for image files. Student acceptance continues to be excellent and a far greater
interest in histopathology was noted to be present during laboratory sessions. The Aperio viewer
(Imagescope) continues to be preferred by students to a virtual slide viewer used in histology.
We have recently updated the image server to allow viewing of images on alternative devices
such as Mac computers and iPads. In addition limited use has been made of Silverlight
technology to allow integrated use of “zoomable” images on Sakai pages.
General Pathology Sequence (in Block 1): The course consists of ten lecture sessions covering
general pathology and six workshop sessions using virtual microscopy and gross organ
demonstrations·. Laboratories were staffed by both Ph.D. and M.D. faculty so as to afford
students the opportunity to meet both research and clinical faculty. Virtual microscopy images
were presented using the image server. It is that the additional workshop and lectures presented
this year provided a more coherent introduction to aspects of pathology necessary for an
understanding of subsequent material. Each laboratory session included a short at home open-
book examto help reinforce major points in the lecture and laboratory.
DENTAL:
First Year Dental School Teaching: Pathology 127: Dr. Reisner (Course Director) provided a
series of nine one hour lectures which cover all essential aspects of general pathology. Because
much of this material is not reviewed in subsequent courses in systemic medical and dental
pathology, a good deal of attention to details and use of the textbook (Rubin's Essentials of
Pathology 5th
Edition) was encouraged. All lecture material was presented as Powerpoints which
are made available to students before the lecture. There are seven laboratories covering general
aspects of histopathology which are supervised by Drs. Hadler (who comments on gross organ
pathology) and Reisner and the expanded use of introductory laboratory "podcasts" has proven
both useful and popular. Two multiple choice exams were used as evaluation tools along with
short "extra credit" exercises added this year to a surprising degree of enthusiasm. In general,
course comments and ratings have continued to be excellent.
43
Second Year Dental School Teaching (Pathology 214): The course is currently a series of eleven
lectures designed to cover most areas of systemic pathology by invited Pathology Clinical
Faculty with Dr. Reisner filling in where necessary. Because of this format we continue to
reduce the variability between sessions. The lack of a laboratory de-emphasizes histopathology
and the use of fixed organ material. Lectures are now much more standardized and apropos the
needs of the Dental students. Given the availability of virtual microscopy short self-directed
laboratory modules may also be included in the future. One sample podcast (in pulmonary
pathology) has been produced for testing purposes.
MOLECULAR AND CELLULAR PATHOLOGY GRADUATE PROGRAM
William B. Coleman, Ph.D., Director of Graduate Studies
Jonathan W. Homeister, M.D., Ph.D., Associate Director of Graduate Studies
The graduate student body of the Molecular and Cellular Pathology Graduate Program
individually and collectively accumulated a number of significant accomplishments during the
past year. Eight students successfully completed the Ph.D. program (Jason Doherty, Rachel
Goldsmith, Mark Gramling, Mehmet Karaca, Troy McEachron, Matt Medlin, Avani Pendse, and
Chih-Hong Wang), and one student completed the M.S. degree (Lisa Samuelson). With these
graduates, the Molecular and Cellular Pathology Graduate Program has produced 166 total
graduates and 119 Ph.D. graduates since 1954. For the most part, the recent Ph.D. graduates
have immediate plans to continue their professional development through postdoctoral research
(Rachel Goldsmith – NIEHS; Mark Gramling – Vanderbilt University; Mehmet Karaca –
NIEHS; Troy McEachron – St. Jude Children’s Research Hospital; Chih-Hong Wang –
University of California at San Diego), additional training (Avani Pendse – Pathology Residency
Program at UNC Hospital), or industrial science positions (Jason Doherty – New Orleans
BioInnovation Center; Matt Medlin – Vascular Pharmaceuticals Inc.). The Biological and
Biomedical Sciences Program (BBSP) continues to admit excellent graduate students, many of
whom are interested in the Molecular and Cellular Pathology Graduate Program. During
Summer 2010, Fall 2010, and Spring 2011, faculty members associated with the Molecular and
Cellular Pathology Ph.D. Program hosted 18 laboratory rotation experiences for 11 individual
students (among 9 faculty laboratories). This was comparable to the success of the 2009-2010
rotations (with 17 laboratory rotation experiences for 12 individual students) and the 2008-2009
rotations (with 18 laboratory rotation experiences for 15 individual students). In June 2011, two
rising second year students officially joined the Molecular and Cellular Pathology Ph.D.
Program, including Pamela Lockyer, and Bethany Walton. These new students will work with
Drs. Cam Patterson, and Alisa Wolberg, respectively. Over the initial three years of the BBSP
the Molecular and Cellular Pathology graduate program has recruited a total of 14 students. In
addition, Julie Gambone (MD, PhD students) joined our program to work with Dr. Nigel
Mackman.
In the period spanning 2010-2011, graduate students contributed to numerous publications in
peer-reviewed journals and published abstracts, many with a graduate student as first author, and
several with multiple graduate students as co-authors. In addition, several graduate students
were recognized for their research excellence with awards. At the 2010 Molecular and Cellular
Pathology Annual Research Symposium (September 2010), Amanda Rinkenbaugh received the
44
award for best poster presentation by a graduate student and Jessica Cardenas received the award
for best oral presentation by a graduate student. Lantz Mackey and Jessica Rodriguez received
Student Travel Awards from the American Society for Investigative Pathology (to attend
Experimental Biology 2011). Research support for students in Molecular and Cellular Pathology
was provided by several sources. Maria Aleman, Adam Phefferle, Amanda Rinkenbaugh, and
Aleeza Roth were supported by the Environmental Pathology Training Program. Dinuka De
Silva was supported by the Cancer Biology Training Program. Kaitlin Lenhart, Jessica
Cardenas, and Jessica Rodriguez were supported by the Integrative Vascular Biology Training
Program. In addition, several students applied for extramural predoctoral fellowships from the
American Heart Association, the Department of Defense, the NIH, or other funding agencies.
Lance Johnson and Kellie Machlus were supported by predoctoral fellowships from the
American Heart Association, and Jessica Cardenas has recently been awarded a predoctoral
fellowship from the American Heart Association. Michael Durando was supported by a
predoctoral fellowship from the NIEHS. Lantz Mackey was supported by a GEM Fellowship.
In addition, several students were supported by funds from the Department of Pathology and
Laboratory Medicine or other units of the UNC Graduate School or School of Medicine. Jessica
Rodriguez was partially supported by the William R. Kenan Jr. Fellowship. During 2009-2010,
two students were recognized as Robert H. Wagner Scholars in Molecular and Cellular
Pathology: Amanda Rinkenbaugh and Mark Gramling. Six Molecular and Cellular Pathology
Ph.D. students (Patricia Casbas-Hernandez, Meghan Free, Amanda Rinkenbaugh, Jessica
Rodriguez, Aleeza Roth, Rupan Sandhu) are HHMI Fellows participating in the Program in
Translational Medicine.
During the last year, the Graduate Student Seminar Series (that began in fall of 2001) continued
to showcase the excellent research of the graduate trainees. During spring 2007, the seminar
series was moved to Tuesday at noon and became a luncheon seminar to enhance attendance.
This modification of seminar schedule has been very successful. The spring 2011 Seminar
Series featured presentations by ten Molecular and Cellular Pathology Ph.D. students, one
Masters student, and one postdoctoral fellow from the Department. Beyond our Tuesday
seminar series, graduate students from our program participated in numerous other research
symposia on campus. Graduate students were also featured in a Pathology Grand Rounds
session in Spring 2010. Jessica Cardenas (from Dr. Frank Church’s laboratory) gave a
presentation entitled “The Role of p16INK4a-Mediated Cellular Senescence in Venous
Thromboembolism,” Maria Aleman (from Dr. Alisa Wolberg’s laboratory) gave a presentation
entitled “Differential Contributions of Monocyte- and Platelet-derived Microparticles Towards
Thrombin Generation and Fibrin Formation and Stability,” and Michael Durando (from Dr.
Cyrus Vaziri’s laboratory) gave a presentation entitled “Pol eta and Rad18 Cooperate to
Facilitate Repair and Replication of Environmentally-induced DNA Damage.” This series
provides a valuable opportunity for students, faculty, and staff to learn more about graduate
student research that is ongoing in the department. In September of 2010, the seventh Marc J.
Mass, Ph.D., Memorial Distinguished Lecture was held, featuring Dr. Tyler Jacks (David H.
Koch Professor of Biology, Director, Koch Institute for Integrative Cancer Research,
Investigator, Howard Hughes Medical Institute, Massachusetts Institute of Technology). Dr.
Jack’s lecture was entitled “Molecular Analysis of Lung Cancer Progression.” In the summer
of 2010, the graduate students selected Dr. Jonathon W. Homeister as the 2010 recipient of the
Joe W. Grisham Award for Excellence in Graduate Student Teaching. The award was
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presented in September 2010 at the home of Dr. J. Charles Jennette. In other activities, the
graduate students have continued to have regular outings to local restaurants for informal
discussions related to the graduate program and their research.
RESIDENCY TRAINING PROGRAM IN PATHOLOGY
Thomas W. Bouldin, M.D., Director
The Department of Pathology and Laboratory Medicine currently sponsors a residency training
program in anatomic and clinical pathology. The Program is fully accredited by the American
Council on Graduate Medical Education (ACGME). A full description of the Program, including
the curriculum and current trainees, is on the departmental web site
(http://www.med.unc.edu/pathology/residency-program-in-pathology /).
The educational goals and philosophy of the residency program are to (1) Provide a flexible,
broad-based training program for physicians that includes training in anatomic, clinical, and
experimental pathology; (2) Encourage trainees to participate in research; and (3) Provide an
educational experience sufficient to ensure that all residents develop skill levels expected of a
new practitioner in the six ACGME-defined competencies (patient care, medical knowledge,
practice-based learning and improvement, interpersonal and communication skills,
professionalism, and systems-based practice.
The Program offers a four-year, combined anatomic and clinical pathology residency, with
ample opportunities for research and post-residency fellowship training in a wide range of
subspecialty areas in pathology. The Program was reviewed by the Residency Review
Committee of the American Council for Graduate Medical Education and was awarded
“Continued Full Accreditation” in October, 2009.
The Program requires that all residents take combined training in anatomic pathology and
clinical pathology. The first three years of the Program are focused on core training in anatomic
pathology (AP) and clinical pathology (CP). The curriculum is organized to intermingle AP and
CP core rotations within each of the first three years of training The fourth year of the Program
permits the trainee great flexibility. The fourth year of the training program includes six months
of elective rotations in AP, CP or pathology research, so that the resident can concentrate on his
or her particular interests. Overall, there are nine months of elective rotations interspersed within
the four-year training program.
The Department of Pathology and Laboratory Medicine has a strong commitment to providing
funding and faculty support for resident research. Funding for resident research projects comes
from a variety of sources, including NIH research or training grants, NIH Individual Research
Service Awards, and other fellowships. To encourage basic research, the Department offers a
one-year research fellowship, available on a competitive basis, to post-residency trainees in the
Department of Pathology and Laboratory Medicine. The fellowship pays a stipend to the resident
commensurate with the fellow’s level of training; and will also provide a $5,000 bench fee to the
laboratory of the trainee’s research advisor. The research must be focused on discovering or
elucidating basic mechanisms of disease. More applied/correlative/clinical research experiences
46
can be obtained in the various clinical fellowships available to pathology residents. Dr. William
Funkhouser is the director of this research fellowship.
The Department provides all residents in the training program with an individual study carrel, a
light microscope, and a computer. The computer is fully loaded with appropriate software,
connected to the internet, and fully supported by the UNC Hospitals’ computer-support staff.
The residency program currently accepts four new residents per year into the four-year general
residency training program. There were 15 residents in the residency program in 2010–11, and
there will be 16 residents in the Program in 2011–12. Additionally, there were two trainees in our
post-residency surgical pathology fellowship program, six trainees in ACGME-accredited
clinical subspecialty residencies (fellowships), and five postdoctoral trainees in clinical
laboratory medicine fellowships (accredited by other agencies) in 2010–11. UNC Hospitals
funded 14 of the training positions in the general residency program in 2010–11, with the
remaining residency and fellowship funding coming from the Department. As of July 1, 2011,
UNC Hospitals will fund 15 of the residency positions.
In 2010–11, 324 applicants applied through the Electronic Residency Application Service
(ERAS®) for the four PGY1 training positions offered. The Department invited 54 of these 324
applicants for an interview, and 43 came to Chapel Hill for an interview. Thirty-nine of these
interviewees were listed in the 2011 National Resident Match, which is conducted by the
National Resident Matching Program. The Department filled all four PGY1 positions in the 2011
Match from within the group of top-listed applicants on the Program’s Match list.
SUBSPECIALTY FELLOWSHIP TRAINING PROGRAM
CLINICAL CHEMISTRY FELLOWSHIP
CATHERINE A. HAMMETT-STABLER, Ph.D., DIRECTOR
LAURA M. BENDER, Ph.D., FELLOW, 2010-2011
STEVEN W. COTTEN, Ph.D., FELLOW, 2010-2011
(http:www.pathology.unc.edu/fellowship/clinchem.htm)
Begun in 1972, this COMACC-accredited postdoctoral training program has a rich history of
producing leaders within the field of Clinical Chemistry. Fellows receive two-years of intensive
training in both the analytical and clinical aspects of clinical chemistry and are prepared to enter
laboratory medicine in clinical service, educational, or research roles. Two fellows began their
training July 2010. Steven Cotton, PhD (Pharmaceutical Sciences, Eshelman School of
Pharmacy, UNC, 2010) was recruited to the UNCH funded position, while Laura Bender, PhD
(Department of Cancer Biology, Wake Forest University, 2005) joined the program as the
recipient of the Past-President’s Scholarship (Van Slyke Foundation of the American
Association of Clinical Chemistry). During this year they have contributed to 4 abstracts, 4
chapters, and 6 manuscripts. The Clinical Chemistry Fellowship is directed by Catherine
Hammett-Stabler, Ph.D., DABCC.
47
CLINICAL MICROBIOLOGY FELLOWSHIP
PETER H. GILLIGAN, Ph.D., DIRECTOR
EDWARD P. AGER, Ph.D., FELLOW, 2010-2011
MOHAMED ELREFAEI, M.D., Ph.D., FELLOW, 2010-2011
The Department of Pathology and Laboratory Medicine and UNC Hospitals sponsors the
Clinical Microbiology Training Fellowship, which is a two-year training program accredited by
the committee on Post-doctoral Education Programs of the American College of Microbiology.
The major objective of this program is to train individuals to direct clinical and public-health-
microbiology laboratories. The fellows’ training includes five areas: (1) Technical training to
become proficient at performing and interpreting the laboratory procedures offered in the clinical
microbiology laboratory; (2) Administrative training in the various aspects of laboratory
management and administration, including budgeting, personnel, quality control, protocol
preparation, safety regulations, and CLIA and OSHA requirements; (3) Clinical training enabling
the trainee to interface effectively with infectious-disease clinicians; (4) Research in clinical
microbiology; and (5) A three week external rotation at the State Laboratory of Public Health.
The Clinical Microbiology Fellowship is directed by Peter H. Gilligan, Ph.D.
CLINICAL MOLECULAR GENETICS FELLOWSHIP
JESSICA K. BOOKER, Ph.D., DIRECTOR
FERRIN WHEELER, Ph.D., FELLOW, 2010-2011
The Department of Pathology and Laboratory Medicine and UNC Hospitals sponsors a Clinical
Molecular Genetics fellowship, which is a one- or two-year training program in laboratory
aspects of clinical molecular genetics. The program is accredited by the American Board of
Medical Genetics. The Molecular Diagnostic Laboratory at UNC Hospitals provides experience
with tests including cystic fibrosis, fragile X mental retardation, hemochromatosis, factor V
Leiden and prothrombin, α1-antitrypsin deficiency, MCAD deficiency, connexin 26 and 30
mutations, Prader-Willi and Angelman syndromes, primary ciliary dyskinesia, EBV, CMV, and
BK viral loads, hereditary cancers, acquired mutations in cancer, chromosomal breakpoints in
leukemias, pharmacogenetics, and monitoring of bone marrow transplants with polymorphic
microsatellite markers. State-of-the-art technologies and instrumentation are used in all of these
tests. The clinical aspects of the training program are complemented by a strong research
foundation. The Clinical Molecular Genetics Fellowship is directed by Jessica Booker, Ph.D.
There was one fellow in the training program in 2010-2011.
CLINICAL MOLECULAR PATHOLOGY FELLOWSHIP
MARGARET L. GULLEY, M.D., DIRECTOR
CHARLES SAILEY, M.D., FELLOW 2010-2011
(http://www.pathology.unc.edu/fellowsp/molecular_path.htm)
The Department of Pathology and Laboratory Medicine and University of North Carolina
Hospitals sponsors a one-year fellowship in Molecular Genetic Pathology. The training program
is accredited by the ACGME to train one fellow annually. Trainees gain a working knowledge
of molecular procedures including Southern blot, in situ hybridization/FISH, DNA sequencing,
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protein truncation test, DNA amplification, tissue macrodissection and other cell enrichment
procedures, and array technologies including gene expression profiling and single nucleotide
polymorphism (SNP) chips. These modern molecular technologies are applied in a wide
spectrum of clinical settings including cancer, inherited disease, infectious disease, HLA-typing,
identification, and pharmacogenetics. The fellow analyzes and interprets molecular data from
clinical cases and composes reports that are relied on for patient management. The fellow learns
to design and carry out research aimed at understanding the molecular basis of disease and
translating fundamental discoveries into improved patient care. Ethical issues, quality assurance,
and lab administration are discussed as they relate to clinical practice. UNC has the longest track
record of board certifications among all ACGME-accredited molecular genetic pathology
training programs. The program is directed by Margaret L. Gulley, MD with support from many
other faculty and staff. More information is found at, (http://www.med.unc.edu/pathology/clinical-fellowships/molecular-genetic-pathology-fellowship).
CYTOGENETICS FELLOWSHIP
KATHLEEN W. RAO, Ph.D., DIRECTOR
The McLendon Clinical Laboratories of UNC Hospitals and the Department of Pathology and
Laboratory Medicine sponsor a fully accredited training program in Clinical Cytogenetics, which
leads to eligibility for certification by the American Board of Medical Genetics (ABMG). The
usual training period is two years. Upon successful completion of the program and ABMG
Certification, the fellow will be qualified to direct a clinical cytogenetics laboratory. The UNC
Cytogenetics laboratory is a full service laboratory, processing over 3600 specimens annually,
for both constitutional and oncology cytogenetic analysis, including CVS, amniocentesis,
peripheral blood, bone marrow, tumors, tissue biopsies, and paraffin sections. Fellows are trained
in a variety of techniques, including tissue culture, chromosome banding and analysis, FISH, and
high resolution chromosomal microarray. The Clinical Cytogenetics Fellowship is directed by
Kathleen W. Rao, Ph.D.
CYTOPATHOLOGY FELLOWSHIP
SUSAN J. MAYGARDEN, M.D., DIRECTOR
YASMIN I. LUTTERBIE, M.D., FELLOW, 2010-2011
KRISTIN A PIERCE, M.D., FELLOW 2010-2011
Cytopathology was fortunate to have two excellent fellows, Dr. Yasmin Lutterbie, M.D. and Dr.
Kristen Pierce, M.D. The fellowship this year was expanded to include immediate interpretations
by fellows for adequacy for most FNAs after November. This provided an opportunity for
graduated responsibility, and freed up the faculty to assist on simultaneous FNAs and other
duties in the laboratory.
FORENSIC PATHOLOGY FELLOWSHIP
DEBORAH L. RADISCH, M.D., MPH, DIRECTOR
JONATHAN D. PRIVETTE, M.D., FELLOW, 2010-2011
The Office of the Chief Medical Examiner (OCME) in conjunction with the Department of
Pathology and Laboratory Medicine and UNC Hospitals, offers a one-year fellowship in forensic
pathology. The program is fully accreditation Council for Graduate Medical Education
49
(ACGME) and is under the direction of the Chief Medical Examiner for the State if North
Carolina. The trainee in forensic pathology performs approximately 250 forensic autopsies
during the course of the one-year fellowship. Consultations in subspecialty areas, including
neuropathology, pediatric pathology, forensic odontology, and forensic radiology, are readily
available within the Department of Pathology and Laboratory Medicine and the School of
Medicine and Dentistry. Ancillary laboratory studies, including clinical chemistry,
microbiology, and special histology, are provided by the Department of Laboratory Medicine.
Forensic anthropology, crime lab technology, and other training experiences are also provided.
The Forensic Pathology Fellowship is directed by Deborah L. Radisch, M.D., MPH. There was
one fellow in the training program in 2010-2011.
HEMATOPATHOLOGY FELLOWSHIP
CHERIE H. DUNPHY, M.D., DIRECTOR
DANA L. CAIRO TUNNELL, M.D., FELLOW, 2010-2011
The Department of Pathology and Laboratory Medicine (McLendon Clinical Laboratories) and
UNC Hospitals sponsor a broadly based, one-year training program in hematopathology. The
program is directed by full-time hematopathologists and is fully accredited by the ACGME. The
program has been highly successful in attracting high-quality applicants with a broad range of
backgrounds, interests, and career goals. As part of a large, prestigious academic department of
pathology, there is a tendency to favor applicants with academic backgrounds and/or
inclinations, although the program itself is primarily training oriented. The departmental
philosophy, which is shared by the Hematopathology program, is that pathology is a diverse field
of endeavor, and that individual contributions may be made in many ways. Hematopathology is a
diverse specialty, employing a wide variety of technologies, and our Fellowship program is
organized in such a way as to provide appropriate training in all of these areas, while providing
flexibility to address personal needs, interests, and objectives of the individual fellows. Trainees
gain experience in the management and medical supervision of a high volume hematology
laboratory (1,000 CBCs/day), the evaluation of peripheral blood smears, bone marrow, and
lymph node biopsies, and involvement in the various procedures, conventionally grouped
together as special hematology.
The following represent specific written competency-based goals and objectives of the
Hematopathology Fellowship at UNC-Chapel Hill: (a) development of proficiency in normal and
abnormal peripheral blood cell morphology, body fluid examination, and bone marrow and
lymph node diagnostics, (b) familiarity with applications of flow cytometric
immunophenotyping to Diagnostic Hematopathology (c) familiarity with procedures, principles,
and quality assurance in analytical hematology, (d) development of familiarity with cytogenetic
and molecular abnormalities in hematolymphoid malignancies, and (e) development of an
approach to clinical and laboratory evaluation of patients with disorders of thrombosis or
hemostasis. The Division of Hematopathology prepares comprehensive diagnostic reports on all
hematolymphoid samples, incorporating morphological, special stains, immunohistochemical,
flow cytometric, cytogenetic, and molecular genetic data. The Hematopathology Fellowship
Program works closely with other laboratory areas to provide practical training in diagnostic
flow cytometry, cytogenetics, blood coagulation, and molecular hematopathology. Fellows
attend regularly scheduled didactic sessions (including “around the scope” didactic sessions and
50
faculty lectures). Fellows also present at and participate in a wide range of regularly scheduled
multidisciplinary, clinicopathologic conferences and are actively involved in interactions with
clinicians, including frequent consultations. They also play a crucial role in teaching
hematopathology to medical students, as well as to pathology and hematology and oncology
residents. The fellow is responsible for the smooth running of the Diagnostic Hematopathology
Service, when assigned to this service, and is actively involved in all specimen work-ups and
diagnostic reports of this service. The fellow is given graduated responsibilities on the
Hematopathology Diagnostic service, depending on their individual level of competence, so that
he/she may practice at the level of junior faculty by the end of the fellowship training.
Supervision is provided on a daily basis by the attending hematopathologist with the Program
Director also continually available and on-site. Competence is measured objectively by the
fellow’s participation in two Hematopathology in-service examinations administered by the
American Society of Clinical Pathology- one at the beginning of the training period (September)
and one at the end of the training period (May). While the fellowship is geared primarily to the
diagnosis of hematolymphoid disorders, research activities are strongly encouraged, with
numerous research opportunities available within the Division of Hematopathology and the
Pathology Department as a whole. The Hematopathology fellows have been very active in
presenting at national meetings and in scholarly activities with resultant journal publications. The
goals and objectives of the Hematopathology Fellowship program are distributed to the fellows.
The Hematopathology Fellowship is directed by Cherie H. Dunphy, M.D. There was 1 fellow in
the training program in 2010-2011. The Hematopathology Fellowship Program currently has
permanent approval from the ACGME for 2 fellowship positions annually.
NEPHROPATHOLOGY FELLOWSHIP
VOLKER R. NICKELEIT, M.D., DIRECTOR
ERIC CAMPENOT, M.D., FELLOW, 2010-2011
The Department of Pathology and Laboratory Medicine sponsors a one-year fellowship in renal
pathology. One or two fellows are accepted into the program. The fellows are directly involved
in the diagnostic evaluation of over 1700 renal biopsies/nephrectomies (both native and
transplant cases) examined annually. All fellows are integrative members of the
nephropathology team and receive intensive training. They prepare cases for sign out by the
faculty using all standard techniques (light microscopy, immunofluorescence microscopy,
immunohistochemistry and electron microscopy). Part of the fellows’ responsibility is to
organize clinico-pathologic and biopsy review conferences for medical faculty and housestaff,
and to teach renal pathology to medical students, residents and fellows. Teaching conferences
and continuous education series offered by the nephrology and transplant divisions at UNC
provide additional ample learning opportunities. Although emphasis is placed on the
development of diagnostic skills, fellows are expected to carry out clinico-pathological and/or
basic research projects and to present their data at national meetings, such as the ASN or
USCAP. Research projects focus on the pathogenesis of glomerulonephritides, allograft
rejection and polyomavirus infections. All state-of-the-art facilities (including laser
microdissection) are available in the department. Appropriate research studies are funded by
intramural support for the division of nephropathology. Clinico-pathological studies are
facilitated by the Glomerular Disease Collaborative Network, which is a well established
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network of over 200 nephrologists participating in clinical data collection. The division of
nephropathology and the fellowship training program is directed by V. Nickeleit, M.D.
NEUROPATHOLOGY FELLOWSHIP
THOMAS W. BOULDIN, M.D., DIRECTOR through December 31, 2010
(http://www.pathology.unc.edu/fellowsp/neuropath.htm)
The Department of Pathology and Laboratory Medicine and UNC Hospitals has sponsored a
broadly based, two-year fellowship in diagnostic and experimental neuropathology since 1980.
The training program has been under the direction of Dr. Bouldin and was fully accredited by the
Accreditation Council for Graduate Medical Education (ACGME). Due to a waning interest in
subspecialty training in Neuropathology by Pathology residents in recent years, the Department
voluntarily closed the fellowship program on December 31, 2010.
SURGICAL PATHOLOGY FELLOWSHIP
WILLIAM K. FUNKHOUSER, M.D., Ph.D., DIRECTOR
DANIEL T. KLEVEN, M.D., FELLOW, 2010-2011
NORRIS J. NOLAN, M.D., FELLOW, 2010-2011
The Department of Pathology and Laboratory Medicine sponsors a one-year fellowship in
diagnostic Surgical Pathology. The training program focuses on surgical pathology, with
correlative exposure to cytopathology, cytogenetics, electron microscopy,
immunohistochemistry, and molecular genetic pathology. During the first 6 months, the fellow
reviews and dictates inside cases on all service benches for 4 months, reviews /dictates outside
cases and gives associated conferences for 1 month, and has 1 month of elective time. The fellow
is credentialed by the hospital during the fall, and repeats the 6 month cycle above as a faculty
instructor, now with independent signout responsibilities. Therefore, 2 months of elective time
are available during the year for completion of a relevant research project. Finally, there is an
option for integration of the Surgical Pathology and Molecular Genetic Pathology Fellowships in
serial order, for the benefit of fellows interested in an academic career in molecular surgical
pathology.
TRANSFUSION MEDICINE FELLOWSHIP
ARABA N. AFENYI-ANNAN, M.D., M.P.H., DIRECTOR
YARA A. PARK, M.D.
POULOMI PAI, M.D., FELLOW, 2010-2011
The Department of Pathology and Laboratory Medicine and McLendon Clinical Laboratories of
UNC Hospitals sponsor a comprehensive one-year fellowship program in Blood
Banking/Transfusion Medicine that is fully accredited by the Accreditation Council of Graduate
Medical Education (ACGME). The training program provides didactic and practical training in
advanced immunohematology, therapeutic and donor apheresis, blood component donation,
testing, preparation and storage, clinical coagulation, histocompatibility, hematopoietic
progenitor cell collections and processing, and clinical support for an academic tertiary care
hospital. Supported clinical programs include transplant programs in marrow/stem cells, liver,
heart, lung and kidney; a Level I trauma program; and a neonatal intensive care unit. Ongoing
52
projects include epidemiology and pathogenesis of thrombotic thrombocytopenic purpura (TTP)
and multiple studies within the NIH funded Transfusion Medicine/Hemostasis Clinical Trials
Network, of which we are one of 17 participating sites. The Transfusion Medicine fellowship is
directed by Araba Afenyi-Annan, M.D., M.P.H. The fellow during 2010-2011 was Poulomi Pai,
M.D. During her fellowship, she conducted a research project aimed at improving
comprehensive care for sickle cell patients in a chronic exchange program. The abstract from her
project was accepted to the annual meeting of the American Society for Apheresis. The abstract
entitled, “Comprehensive Review of Sickle Cell Disease Patients on Chronic Exchange
Transfusion Therapy Improves Patient Management”, was presented as an oral abstract
presentation.
GRAND ROUNDS SEMINARS 2010-2011
GRAND ROUNDS ORGANIZING COMMITTEE: MARGARET L. GULLEY (CHAIR),
MEMBERS: THOMAS W. BOULDIN, M.D. and JOE N. KORNEGAY, M.D.
As has been the case in years past, the Department of Pathology and Laboratory Medicine Grand
Rounds seminar series was well attended during the academic year 2010-11. The primary goals
of this series is twofold: 1) to provide a venue for the dissemination of current basic science and
clinical research information relevant to departmental academic activities and 2) to promote
interaction and the opportunity for collaboration between Pathology faculty, residents,
postdoctoral fellows, graduate students, and clinical fellows, and other members of the UNC
community. Additionally, we use Grand Rounds as a venue for faculty presentations needed as
part of promotion and post-tenure reviews and as a forum for announcements and discussion of
items of interest and importance to faculty and trainees.
To accommodate speaker and audience needs, Grand Rounds follows a flexible format. The
presenters may choose a traditional format in which there is a single presenter; or when
appropriate, as when integrating basic and clinical research or two or more disciplines, some
choose to share the time with a collaborator or trainee. Presentations are usually 45 minutes,
followed by a question-and-answer session. The committee strives to assure a range of
experimental, clinical and surgical pathology subjects are appropriated and evenly covered. The
topics are dependent upon speaker availability and while many presentations are usually related
to the presenter’s research interests, some include scientific reviews of pertinent areas in clinical
medicine, translational research, and/or basic science. The following list of 2010-11 presenters,
their affiliations and topics demonstrate that both internal and external speakers are sought.
Category 1 CME credit is offered for seminar participation. We provide an opportunity for the
speakers to have their presentation formally evaluated, as required of all CME activities. Written
comments and questions concerning the quality of the presentations are requested. Prior to each
Grand Rounds seminar, refreshments are provided. This encourages a collegial atmosphere, and
it also provides an opportunity for the attendees to visit and discuss science, medicine, and
research.
FALL 2010
DATE SPEAKER/AFFILIATION TITLE
08/26/2010 Harsharan K. Singh, MD
Associate Professor, Dept. of Pathology
“Haufen are Novel Urinary Biomarkers
of Polyomavirus Nephropathy: The
Rocky Road from Bench to Bedside”
53
and Laboratory Medicine, UNC-CH
09/16/2010 Kathleen A. Kaiser-Rogers, PhD
and Laboratory of Medicine, of Pediatrics,
and of Genetics, UNC-CH
“The Impact of Microarray Testing on
The Field of Clinical Cytogenetics”.
09/23/2010 Masao Kakoki, MD, PhD
Assistant Professor, Dept. of Pathology
and Laboratory Medicine, UNC-CH
“Bradykinin, Diabetic Complications
and Senescence”
09/30/2010 Thomas H. Fischer, PhD
Associate Professor, Dept. of Pathology
and Laboratory Medicine, UNC-CH
“Rehydrated, Lyophilized Platelets for
Hemostasis”
10/21/2010 Joe N. Kornegay, DVM, PhD
Professor, Dept. of Pathology and
Laboratory Medicine, UNC-CH
“Translational Lessons Learned from a
Canine Model of Duchenne Muscular
Dystrophy”
10/28/2010 Christopher R. McCudden, PhD, DABCC,
NRCC, FACB
Assistant Professor, Dept. of Pathology
and Laboratory Medicine, UNC-CH
“Monoclonal Gammopathies: New
Treatments, New Challenges”
11/11/2010 Nancy L. Allbritton, MD, PhD
Professor and Chair, Joint Dept. of
Biomedical Engineering, UNC-CH and
North Carolina State University
“UNC/NCSU Biomedical Engineering
& Microfabricated Analysis Systems”
11/18/2010 Fernando Pardo-Manuel de Villena, PhD
Associate Professor, Dept. of Genetics
and Director, Program in Systems
Genetics, UNC-CH
“Genetic Analysis of Complex Traits in
the Emerging Collaborative Cross”
12/09/2010 James A. Swenberg, DVM, PhD
Professor, Depts. of Environmental
Sciences and Engineering, of Pathology
and Laboratory Medicine, and of
Nutrition, UNC-CH
“Inhalation-specific and Endogenous
Formaldehyde DNA Adducts: Testing
the Biological Plausibility of Leukemia”
SPRING 2011
DATE SPEAKER/AFFILIATION TITLE
01/06/2011 Susan S. Smyth, MD, PhD
Professor of Cardio Vascular Medicine,
Depts. of Internal Medicine, of
Physiology, and of Molecular
Pharmacology, The University of
“Lysophospholipid Signaling in the
Vasculature: Implications for Health
and Disease”
54
Kentucky and Lexington VA Medical
Center
01/20/2011 William K. Kaufmann, PhD
Professor, Dept. of Pathology and
Laboratory Medicine, UNC-CH
“Cancer, Genetic Instability and the
DNA Damage Response”
01/27/2011 Gregg A. Dean, DVM, PhD, DACVP
Professor and Director, Center for
Comparative Medicine and Translational
Research, College of Veterinary
Medicine, North Carolina State University
“Innate Immune Defects and AIDS-
Related Opportunistic Infections: What
Can we Learn from the FIV/Cat
Model?”
02/10/2011 George (Yuri) Fedoriw, MD
Assistant Professor, Dept. of Pathology
and Laboratory Medicine, UNC-CH
“BAFF and B-cells in Hematolymphoid
Disorders”
02/17/2011 Nizar Chahin, MD
Clinical Assistant Professor, Dept. of
Neurology, UNC-CH
“Pathological Classification of
Inflammatory Myopathy”
02/24/2011 Monte S. Willis, MD, PhD
Assistant Professor, Dept. of Pathology
and Laboratory Medicine, UNC-CH
“Muscle Ring Finger-1 Regulation of
Cardiomyocyte Size and Oxidative
Metabolism by Its Interactions with
Nuclear Receptors”
03/17/2011 Kristy L. Richards, MD, PhD
Assistant Professor, Depts. of Medicine
and of Genetics, UNC-CH &
Steven E. Suter, VMD, PhD, DACVIM
Assistant Professor, Dept. of Oncology
and Medical Director, Canine Bone
Marrow Transplant Unit, North Carolina
State University
“Teaching the Old Dogs New Tricks:
Comparative Oncology in the Genomic
Era”
03/24/2011 Arlin B. Rogers, DVM, PhD
Assistant Professor, Dept. of Pathology
and Laboratory Medicine, UNC-CH
“Biology of Sex-Dependent Liver
Cancer”
03/31/2011 Kevin Gardner, MD, PhD
Senior Investigator, National Cancer
Institute, Bethesda, MD
“Transcriptional Regulation of the
BRCA1 Early Onset Breast Cancer
Gene by a Metabolic Switch”
04/14/2011 Jessica C. Cardenas, PhD, Candidate
Pathology and Laboratory Medicine
Graduate Student Research Day
“The Role of p16INK4a-Mediated
Cellular Senescence in Venous
Thromboembolism”
55
Frank C. Church, Advisor
Maria M. Aleman, PhD Candidate,
Alisa Wolberg, Advisor
“Differential Contributions of
Monocyte- and Platelet-derived
Microparticles Towards Thrombin
Generation and Fibrin Formation and
Stability”
Michael L. Durando, PhD Candidate,
Cyrus Vaziri, Advisor
“Poleta and Rad18 Cooperate to
Facilitate Repair and Replication of
Environmentally-induced DNA
Damage”
04/21/2011 Pathology and Laboratory Medicine
Residents and Fellows Research Day
“Expression Profiling of Paraffin-
embedded Lymphoepithelioma-like
Natalie Banet, MD
AP/CP Resident, PGY3
Carcinoma of the Uterine Cervix using
Nanostring Arrays Reveals no Evidence
of Epstein-Barr Virus Infection”
Jayson R. Miedema, MD
AP/CP Resident, PGY2
“Image and Statistical Analysis of
Melanocytic Histology: A Novel
Technique in a Challenging Area”
Poulomi J. Pai, MD
Transfusion Medicine Fellow, PGY5
“Optimizing Care in Sickle Cell
Patients on Chronic Exchange
Transfusion Therapy”
05/12/2011 Ethan J. Anderson, PhD
Assistant Professor, Depts. of
Pharmacology & Toxicology &
Cardiovascular Sciences, Brody School of
Medicine, East Carolina Heart Institute
“PUFAs, H202 and Ca2+: Mediators of
Cardiac Health and Disease that
Converge on the Mitochondria”
05/19/2011 Caterina M. Gallippi, PhD
Assistant Professor, Joint Dept. of
Biomedical Engineering, UNC-CH and
North Carolina State University
“Pre-Clinical and Clinical Acoustic
Radiation Force (ART) Ultrasound
Imaging”
ENVIRONMENTAL PATHOLOGY TRAINING PROGRAM
The Environmental Pathology Training Program seeks to develop scientists who discover
mechanisms by which environmental substances affect cellular processes to cause disease. The
program trains scientists to combine an understanding of the pathogenesis of human diseases and
expertise in appropriate research methods to study these diseases. The research focus of the
program is on the mechanisms of pathogenesis of diseases for which environmental exposures
56
are critical factors. Traditional program strengths included the role of environmental factors in
the pathogenesis of cancer and their role in DNA damage and repair, reflecting the expertise of
the faculty mentors. This program, which is currently in year 35 of support from the National
Institute of Environmental Health Sciences, has 6 slots for postdoctoral fellows and 6 slots for
predoctoral trainees. During this past year all training slots on the Environmental Pathology
Training Program were filled.
Some postdoctoral candidates learn about our training program by viewing our website, by
reading our advertisements in national journals, or through interviews at job placement services
of national societies. Others candidates have been referred to the program by prominent
investigators in environmental pathology. The program gained a national recognition as one of
the premier postdoctoral training programs in this field. Trainees accepted by the program
typically come from fine graduate programs in related fields from around the country. Unique
features of this Program were that a number of our trainees have had M.D. or D.V.M. degrees or
were members of underrepresented minority groups. Several trainees supported by this
institutional grant have subsequently obtained individual postdoctoral research training grants.
In recent years three postdoctoral trainees have received individual training grants from the NIH
and the DOD, and another has received a K08 Award from the NIH. Trainees typically have
found appropriate transitional positions and permanent jobs. One of the trainees recently
completing support from the program went directly to a faculty position in environmental
sciences. Predoctoral trainees are chosen from among the applicants accepted into the
IBMS/BBSP graduate program who select a Program faculty member as their mentor. Most of
them have chosen to seek their Ph.D. in Molecular and Cellular Pathology. Predoctoral trainees
are selected from among those expressing an interest in environmental research projects of the
training program mentors. Typically, one or two predoctoral students are chosen per year,
usually the best among the candidates interested in the work of training grant preceptors.
In May 2009, a competitive renewal application was submitted for the Environmental Pathology
Training Grant. That application did not receive a good enough priority score to be funded, but
an additional year of trainee support was provided to continue the Program while a revised
application was prepared. An extensively revised application attempting to address the
reviewers’ criticism was prepared for submission in May 2010. Because the prior criticism
thought that there was too much emphasis on environmental carcinogenesis the membership of
the Training Program was broadened to include virtually all NIEHS awardees on campus whose
work on environmental studies focused on pathogenesis. In the 2010 application many new
NIEHS-funded investigators were added so that only 25% of the faculty members in the 2010
application had been participants in the 2005 application. Several new areas of environmental
research had been incorporated (pulmonary, renal, and cardiovascular diseases influenced by
environmental factors) to dilute the focus on environmental carcinogenesis. Despite vast
changes in the revised grant application the review did not get a good enough priority score to be
funded. We believe that the reviewers thought that the research being pursued by the selected
postdoctoral, and more importantly, predoctoral trainees was not sufficiently focused on the role
of environmental factors in the pathogenesis of diseases. Consequently, the Training Grant will
be terminated.
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The NIEHS appears to be inclined to support trainees with further eligibility for NRSA training
for at least one year beyond their previous appointment date if it falls before June 30, 2011. We
will not, however, be able to appoint new trainees to fill slots that become open. It remains
unclear whether it will be possible to renew support for trainees after June 30, 2011. A budget to
support the eligible trainees has been submitted to the NIEHS and we are awaiting the official
approval of the plan.
CLINICAL SERVICES
BACKGROUND McLENDON CLINCAL LABORATORIES
HERBERT C. WHINNA, M.D., Ph.D., DIRECTOR
McLendon Clinical Laboratories provides laboratory and pathology services to physicians in
support of excellent patient care at UNC Hospital. Each laboratory section maintains fiscal
accountability for revenue generated and expense required to produce clinical test results. The
revenue contribution from the laboratory has continued to grow, despite the difficult financial
climate facing health care as a whole. The directors of each laboratory, working closely with the
assistant administrative directors, develop short and long range plans to assure that the
laboratories are supporting the testing needs of the hospital, while continuing to provide the
medical staff with cutting edge technologies. For FY 10-11, the laboratory is projected to
contribute 84 million dollars to UNC Hospital’s operating margin.
Outreach has created a Client Services area that began operation in April. This consolidated area
provides customers with one point of contact for McLendon Laboratories. This has been a great
benefit to the Core laboratory and the Microbiology Laboratory as it frees technologists to focus
on testing rather than answering the telephone. Outreach has also brought the courier activity in
house and has branded the vehicles and provided uniforms with the McLendon Laboratory logo
for the drivers. Service to customers has improved and customers are now aware of the entity
providing the service. A new marketing position has been added and marketing efforts will begin
in June.
McLendon Laboratories is in the process of implementing several IT initiatives that, when
completed, will greatly enhance service by decreasing turnaround time and automating
processes. In Surgical Pathology, the implementation of Advanced Bar Coding and Tracking
software will provide the last component of a completely bar coded system, from specimen
grossing through the cutting and staining process to the sign out of the report. Autoverification
has been completed in the Core Laboratory for Hematology and Blood Gases reducing reporting
time and decreasing the opportunity for transcription errors.
SURGICAL PATHOLOGY (Histology/Special Procedures Labs)
WILLIAM K. FUNKHOUSER, Jr., M.D., Ph.D., DIRECTOR
UNC Surgical Pathology generates diagnoses on UNCH specimens, on specimens to be
reviewed because of patient referral to UNC hospitals, and on outside expert consultations
specimens. In 2010, 28,438 cases were diagnosed, including 2819 outside cases, representing an
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unchanged year-over-year faculty caseload. Increasing case complexities make it challenging to
sign out all cases with residents each day, so we expanded 5 non-Derm SP benches to 7 non-
Derm SP benches in Fall 2010, as follows: The Breast/Benign GYN bench was split into
separate Breast and Benign GYN benches. The GI/Liver bench was split into separate GI/Liver
small biopsies and GI/Liver resections. The separate Dermpath bench (the 6th
SP bench), mostly
melanoma re-excisions, was folded into the Dept. of Dermatology.
New faculty members joined us in 2010, including sign-out faculty (Drs. Greene, Scanga,
O’Connor, and DiFurio) and PA faculty (Ms. Kemper, Mr. Holmes). Dr. Greene signs out GI
pathology and Cytopathology. Dr. Scanga signs out GYN, ENT, and Cytopathology. Drs.
O’Connor and DiFurio sign out Breast, GYN, and Cytopathology. We said farewell to Ms.
Boland in May 2010, when she changed to a PA job at Duke, and hired experienced gross room
PAs from Rex (Ms. Kemper) and Duke (Mr. Holmes). We continue our system for credentialing
of Surgical Pathology Fellows in the fall, with transition to final signout responsibility as
Instructors in the spring. This gives us flexibility in the spring to cover faculty extended leave or
departure.
Goals for 2011 include validation of a remote-controlled microscope for doing off-site frozen
section diagnosis, installation of an X-ray machine for selective sampling of breast specimens,
and evolution to seamless barcoding of cases from accessioning to case signout.
The histology laboratory is commensurately busy. We are fortunate that the Laboratory is well-
led by Ms. Maglione, and that it is well-managed by Ms. Deloney. This laboratory and its
upstream accessioning personnel are critical to an efficient, error-free service. Block volume
increases have been met with increased productivity, Lean analysis, improved instrumentation,
and budget approval for seamless barcoding of specimens from accessioning to case signout.
Lean analysis of immunohistochemistry workflow has reduced turnaround time for receipt of
immunohistochemical stains. Challenges for 2011 are to automatically measure block volumes,
case TATs, and error rates, and to correlate these data with staffing type and levels, in order to
define optimal technical staffing.
Overall, continuing increases in workload have been met by continuing increases in effort,
ingenuity, and efficiency. The management and leadership skills of Dr. Whinna, the Director of
the McLendon Clinical Laboratories, and of Dr. Jennette, Chair of the Department of Pathology
and Laboratory Medicine, are perceived as critical to the improvements and successes described
above.
CYTOPATHOLOGY
SUSAN J. MAYGARDEN, M.D., DIRECTOR
The cytopathology laboratory workload and cytotechnology staffing has remained stable.
Cytotechnologists continue to assist in adequacy assessments for fine needle aspirations
performed by radiologists and surgeons, and during this year have gained sufficient experience
and confidence to make adequacy assessments for axillary lymph nodes and thyroid cases
independently. This has improved turn-around time for multiple concurrent procedures. Our two
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cytology fellows, Dr. Kristin Pierce and Dr. Yasmin Lutterbie, have performed in an exemplary
manner. Both will complete the fellowship on June 30, 2011.
The UNC cytotechnology school has been closed by the university because of budgetary issues,
and the director, Mr. Allen Rinas, is retiring June 30, 2011. The cytopathology laboratory is in
the process of moving part of its operations into the cytotechnology school space and expanding
the prep portion of the lab to accommodate new equipment.
We were fortunate to add four new faculty members in cytology (all of whom participate in the
surgical pathology service as well): Drs. Lori Scanga, Kevin Greene, Siobhan O’Connor and
Megan DiFurio. They join Drs. Susan Maygarden and Debra Budwit.
AUTOPSY PATHOLOGY
LEIGH B. THORNE, M.D., DIRECTOR
We recognize the autopsy as a valuable medical procedure for assessing quality of patient care,
evaluating clinical diagnostic accuracy, determining therapeutic effectiveness, increasing
understanding of pathobiology, augmenting clinical and basic research, and for medical
education. The autopsy is used as a critical investigative tool to discern the cause and natural
history of disease, to educate medical students and residents, and as an integral component of
patient care. The large variety of cases encountered provides a rich learning environment.
UNCH Autopsy Service continues to provide valuable information to clinicians and families of
patients. In 2010, a total of 121 autopsies were performed including 35 pediatric cases. A
multidisciplinary committee was formed in 2009-2010 to address issues with decedent care in
general. The mission is to improve not only the autopsy services provided to families of
deceased patients but to improve the process from the time the patient passes to release of the
body to the funeral home. Ultimately, the goal is to create a decedent care coordinator to
facilitate the entire process. The committee continues to advocate the development of this
program which has not yet been funded. In addition to our clinical mission, the service continues
to serve as an important resource for researchers at UNC. In this fiscal year, the breast cancer
rapid autopsy program will be revived, with support provided through grants from Dr. Lisa
Carey.
MOLECULAR PATHOLOGY
MARGARET L. GULLEY, M.D., DIRECTOR
The Molecular Genetics Laboratory performs assays on DNA or RNA to aid physicians in
diagnosis, monitoring, and treatment of infectious disease, cancer, and heritable conditions. A
test menu with description of each clinical service is found on our website:
http://labs.unchealthcare.org/directory/molecular_pathology/index_html. Research and
development is an important component of our clinical and academic mission to advance
healthcare using modern molecular technologies. Our training programs educate physicians,
medical students, post-doctoral fellows, genetic counseling students, and clinical laboratory
science students enrolled in the Masters in Molecular Diagnostic Sciences program. Our
fellowship training program in Molecular Genetic Pathology was the first in the nation to educate
a board-certified physician in this subspecialty. We offer a month-long course in Molecular
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Diagnostics and Cytogenetics that is targeted at pathology residents and also accepts a wide
range of interested medical professionals. Further information on our clinical, educational and
research efforts in molecular pathology is found at:
http://www.med.unc.edu/pathology/faculty/biosketch-of-dr-margaret-gulley
Molecular pathology is growing rapidly as clinicians increasingly use molecular tools for
diagnosis and management. Clinical assays added to our test menu in the past year include:
1. IDH1 and IDH2 mutations to 1) assess prognosis in glioma (all grades), 2) assist in
differential diagnosis of glioma and other neoplastic or reactive lesions in brain tissue,
and 3) assess prognosis of AML in conjunction with other clinicopathologic variables.
2. A novel cytomegalovirus (CMV) DNA viral load assay to measure the virus in plasma
specimens.
3. MGMT P450 2C19 (CYP2C19) gene promoter methylation to predict outcome in a high
grade glioma (anaplastic astrocytoma or glioblastoma) patient being considered for
therapy with an alkylating agent, or glioma patient being considered for temozolamide
therapy.
4. Gene expression profiling, and gene copy number measurement using ultradense arrays
targeting every human gene (Affymetrix or Agilent platforms) or medium density Q-PCR
panels measuring up to 384 genes or transcripts at once (Roche LC480 platform),
including the potential to target human and pathogen genes in the same test panel. These
assays are used in clinical trials and, once validated to be analytically sound and clinical
useful, they may be implemented in CLIA-certified labs to help make medical decisions.
We thank UNC Hospitals, the TraCS Institute, the University Cancer Research Fund, and the
Department of Pathology and Laboratory Medicine for making available the resources to
implement modern molecular technologies and to validate these novel assays. Major Equipment
in the clinical molecular genetics lab: Roche LightCycler 2.0 and 480 real-time PCR
instruments, Roche MagnaPure extractor and MagnaLyser, Perkin Elmer Janus Robotic Pipettor;
Qiagen EZ1, Qiacube, and QiaSymphony extractors; Applied Biosystems 9700, 9800, 7500, and
7900 PCR instruments; two ABI Veriti thermocyclers, Idaho Technologies LightScanner, three
ABI 3130xl capillary gel electrophoresis instruments, Biotage Pyromark MD pyrosequencer,
Agilent array scanner, Affymetrix array scanner, RoboSep, and UVP gel documentation system.
Faculty are Margaret L. Gulley MD, Karen Weck MD, Bill Funkhouser MD PhD, Leigh Thorne
MD, Jessica Booker PhD, Maimoona Zariwala PhD and Rosann Farber PhD. The fellows as of
July 1 2010 are Chuck Sailey MD and Ferrin Wheeler PhD. Our excellent staff includes six
medical technologists, three research scientists, a supervisor, and an office support assistant.
TRANSFUSION MEDICINE, APHERESIS, TRANSPLANT SERVICES
Transfusion Medicine (Blood Bank, Platelet Donor Program, Apheresis)
YARA A. PARK, M.D., DIRECTOR
The Transfusion Medicine Service (TMS) was inspected and reaccredited by CAP, AABB, FDA,
and FACT within the past year. Dr. Yara Park assumed the role of Medical Director of TMS.
Using data from our performance improvement process, TMS added myomectomies as a type
and screen procedure to the Standard Surgical Blood Ordering Schedule. TMS recently
completed a comprehensive computer upgrade which included an operating platform change.
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The Apheresis unit saw increased procedure volumes, particularly in hematopoietic progenitor
cell (HPC) collection which were increased ~30%. Additionally, Apheresis, in conjunction with
the Bone Marrow Transplant program, has been approved to be a National Marrow Donor
Program apheresis collection site to collect HPC products from volunteer donors. All physician
apheresis procedure notes are now being done in WebCIS to allow easier access to the
information. The Blood Donation Program began to incorporate patients in the recognition of the
donation program by having the t-shirt design be one that was created by a patient or family
member and plan on this being an annual event. Currently, the Blood Donation Program is
preparing for a major computer upgrade.
CLINICAL MICROBIOLOGY, IMMUNOLOGY LABORATORIES
PETER H. GILLIGAN, Ph.D., DIRECTOR
The Clinical Microbiology/Immunology Laboratory has continued to expand its test menu and
test volumes through the addition of new assays and new instrumentation. We have been able to
enhance service to our clinicians and patients while maintaining our training mission. One of the
major accomplishments of Dr. John Schmitz, in the Immunology section, was the modification
of our HIV testing algorithm with the implementation of the new HIV Ag/Ab Combo test. This
test detects both HIV antibody and p24 antigen simultaneously allowing the laboratory to better
detect acute HIV infection while maintaining excellent detection of chronic infection. This new
algorithm eliminated the tedious task of “pooling” HIV negative sera for HIV quantitative PCR
testing to detect acute infection significantly improving turnaround times. This same technology
has also replaced the standard rapid HIV test for blood/body fluid exposures to provide the
benefit of detecting acutely infected source patients.
The Immunology section also expanded our allergy testing menu by establishing a new
childhood allergy panel. This panel is marketed to General Practitioners to improve diagnosis
and treatment of patients that present to primary care clinicians with allergy-like symptoms.
Before the implementation of this new panel, we have seen a 24% increase in test volume from
this time last year.
The Immunology section added new instrumentation, the Diasorin Liaison. This system, our
third random access platform enables us to run 12 assays that were previously performed on an
outdated, batch mode instrument, or were done manually. This system also allowed us to bring
some testing in-house that was previously sent to a Reference Laboratory.
Under the guidance of Dr. Melissa Miller in the Molecular Microbiology section, another major
accomplishment was the implementation of a molecular test for qualitative detection of
Mycobacterium tuberculosis complex DNA by PCR. Rapid identification of Mycobacterium
tuberculosis complex DNA in smear positive samples is useful in both beginning appropriate
therapy for an individual patient and for instituting appropriate infection control measures on a
hospital and community basis.
They were also able to offer a new, rapid PCR test for the laboratory diagnosis that detects both
Influenza A and B viruses. Results are available 7 days/week, 24 hrs/day with a targeted
turnaround time of 90 minutes upon receipt of the specimen in the laboratory. We were also able
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to offer a “traditional” Influenza PCR test for inpatients and those locations that did not require a
rapid turnaround time. During the 2010-11 “flu season” (Dec-April), the laboratory staff
performed 1,025 Rapid Flu tests, 1,385 traditional flu tests and 2,262 Respiratory Viral Panel
molecular tests.
The Molecular Microbiology section brought in new instrumentation, the Abbott M2000 system.
This improved platform replaced the Roche Ampliprep system. This system fully automates
HIV, HCV, HBV improving workflow and turnaround times.
The PNA FISH Yeast Traffic Light test was implemented in our Specimen Processing/Rapid
Procedures area for rapid identification of Candida species from positive blood culture bottles.
The test is performed on slides using specimens taken directly from positive blood culture
bottles. The ability to rapidly report Candida albicans by PNA FISH allows clinicians to safely
treat patients with fluconazole instead of with one of the more expensive anti-fungals. The
estimated institutional cost savings is $1,800 per candidemia patient. When comparing results of
positive patients before and after implementation of PNA-FISH, Candida species were identified
4 days sooner with PNA-FISH and antimicrobials changes, if needed, could be made
approximately 2 days sooner.
They worked very closely with the Emergency Department physicians to review data from
patients that had a urine sample submitted for both urinalysis and urine culture. Their goal was to
see if we could reliably use urinalysis results to limit unnecessary urine cultures (i.e. screen out
the “negatives”). As a result of these efforts, Urinalysis with Culture Reflex testing began in the
Emergency Department on April 11, 2011. In patients meeting certain criteria, a clinician in the
ED may order a “urinalysis with culture reflex” test. The urinalysis is performed first and only
set up for culture if defined parameters are positive. This has been met with great enthusiasm by
the clinicians in that setting.
Finally, they continue their extensive training and educational mission within the laboratory.
They taught a full complement of Clinical Laboratory Science students at both the BS and MS
levels. They continue to train our clinical pathology residents and fellows in both Medical
Microbiology and Medical Laboratory Immunology. They had the largest number of students
ever in the Clinical Pathology elective, PATY 417. Their daily infectious disease teaching
rounds are a popular component of the training of fellows in that discipline. Finally they continue
our clinical microbiology training of the pediatric residents twice weekly.
PHLEBOTOMY SERVICES
PETER H. GILLIGAN, Ph.D., DIRECTOR
In July of 2010, Phlebotomy Services began the new fiscal year having completed the previous
fiscal year under budget. With the arrival of the new house staff we saw some increase in
utilization of the 4AM draw causing delays. This was fairly quickly remediated by contacting
the chief residents and nurse managers and asking them to “coach” the interns on how best to
utilize our service. They have noticed greater utilization on the 10PM draw. Currently, they
average 58.3% of test results from the 4AM draw available by 6AM and 96.3% available by
8AM. On May 1, 2011 we adjusted their staffing to improve turn-around-time of the 4AM
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collections. They determined that their overnight shift (10PM-7AM) only averaged 2-3
collections between Midnight and 3AM. They eliminated the overnight shift and redirected
those employees to the 3 AM shift.
Phlebotomy Services worked successfully with ISD to get the linear barcode that is available on
the patient armband formatted for use with our phlebotomy scanners. Previously, they were
using the 2D barcode containing the billing number on the armband and unable to use the patient
MR for unique patient identification. They are now able to use the MR number. Also, the issue
of the 2D barcode being too small for scanning has been resolved. The linear barcodes are larger
and more clearly printed on the armbands. This increases patient safety and decreases the time
spent by the phlebotomist in automating the identification process.
Blood Culture contamination rates continue to be a source of pride for Phlebotomy Services.
Currently, they average a total BC contamination rate for Phlebotomy Services of 0.67%. Total
contamination rate house wide is currently averaging well below 2%, our institutional bellmark.
Phlebotomy Services was recognized at the March 15, 2011 Department Heads Meeting as
having the lowest Blood Culture contamination in the nation. In addition, to low contamination
rates for Blood Cultures, they have also decreased our average of unjustified delays in collecting
blood cultures to 0.5%. Therefore, only 0.5% of the time is a blood culture delayed due to a
reason within the control of Phlebotomy Services.
Since December 2010, Phlebotomy Services has participated on a Carolina Care team focused on
improving patient responses for the question “courtesy of the person who took blood” on the
Press-Ganey patient survey. The team has focused on identifying the percentage of nurse
collections vs. phlebotomy collections, and identifying strategies such as allowing the unit NA’s
to wake the patients and perform vitals along with the blood collection so that patients are not
disrupted multiple times. Also, by created Words that Work, or phrases that can standardize
communications and responses to patients in order to help improve the scores.
Phlebotomy Services participated in the design and pilot training of a new “Customer Service
101 and 201” course, which will soon be required for all staff. They were happy to be asked to
assist in the design of the program and its beta testing.
Since September 2011, Phlebotomy Services has been involved with the Materials Procurement
Committee and Infection Control in evaluating the current butterfly wing-set devices for safety.
In December 2009, they switched to the SMITHS device, it being “in vein” safety activated.
Unfortunately, the device is not easy to use and has resulted in increased needle sticks among
nursing staff that do not use these devices as frequently as Phlebotomy Services. They have
participated in the evaluation of another device and have agreed to change our device to
accommodate nursing despite the fact that their staff have had no trouble adapting to the
SMITHS device and have not experienced increased needle sticks with it.
They just completed training the UNC second year medical students on how to obtain a blood
specimen by venipuncture during their clinical cases course which is the capstone of their 2nd
year. They have engaged in this program since 2005. The students appreciate the opportunity to
have a phlebotomist give them the tools of the trade and to observe their practice session. They
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also continue to provide phlebotomy clinical training for students from four local community
colleges, and for any clinical personnel including nurses or residents who request it.
Phlebotomy Services is also involved in several projects that are just getting underway to
improve service to our outpatients. The first, is working with the staff in the Oncology Clinics
on patient throughput. Infusion patients will now have their blood drawn by nurses from
indwelling ports in the phlebotomy lab. Phlebotomy will assist with the paperwork to facilitate
moving the patient through the system. Second, they are working with the HUB registration
staff to design a more patient friendly flow at the ACC. Finally they will be working with ISD
and the Transplant Clinic to improve the throughput of their patients by piloting advance
outpatient orders in the hospital computer.
CORE LABORATORY (Chem/UA/Coag/Hem/Tox/Endo)
CATHERINE A. HAMMETT-STABLER, Ph.D., DIRECTOR
All sections of the Core Laboratory services (clinical chemistry, hematology, coagulation,
urinalysis, special coagulation, and referral testing) have seen continued growth in the 2010-2011
fiscal year. The area receives 4500 samples per day, and expects to report >5 million test results
this year. The introduction of the enGen Laboratory Automation System Quality at the end of
the last fiscal year provided opportunities to focus on quality improvement initiatives. These
include Six Sigma projects to reduce in-processing time for all samples and streamline the
processes involved in electrophoresis interpretation and reporting. The introduction of genetic-
based screening for cystic fibrosis as part of the newborn screening program in North Carolina
resulted in the expected increase in sweat chloride confirmation testing. A quality initiative
undertaken with phlebotomy demonstrated a 97.5% success rate in the collection of an adequate
sample from this population. The laboratory is completing the validation of the new IRIS
urinalysis system with enhanced reporting software that will allow for more efficient results
reporting. Additionally, a study streamlining urine culture workflow has been initiated between
the divisions of infectious disease, microbiology, and the emergency department.
Autoverification was introduced into the hematology area to improved efficiency and reduce
turn-around-times. The section has completed evaluation of the Cellavision automated digital
cell morphology system and plans to introduce the system in the following months. Special
chemistry has developed and introduced several new LC-tandem MS-based methods including
vitamin D and buprenorphine and metabolite. Methods for opiate confirmations and free T3’s
are in the final stages of development and evaluation. The Laboratory expanded the ambassador
program with the selection and training of 6 new ambassadors to service units within the
hospital. Twenty technologists began participating in the new mentoring program. The
continuing education program was expanded to provide 2-3 opportunities each month. Marsha
Owens received the first CARE Award for Excellence.
HEMATOPATHOLOGY
CHERIE H. DUNPHY, M.D., DIRECTOR
The volume and complexity of cases has continued to increase in this Division since the recent
move into the recently completely, state-of-the-art North Carolina Cancer Hospital. The annual
in-house bone marrow volume is >2,000 and the lymphomas-evaluation case volume is
approximately 750. Outside review of Hematopathology diagnostic cases has also continued to
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increase as these reviews are necessary for patients being referred to UNC for therapy.
Additional immunohistochemical and flow cytometric markers are continuously being added to
the diagnostic repertoire for this Division, which remains on the cutting-edge of diagnostic
Hematopathology. The Division looks forward to incorporating 6-color flow cytometry into
clinical practice, which will allow better definitions of hematolymphoid neoplasms on
conceivably smaller numbers of neoplastic cells. The Division has benefitted from the new
faculty additions, including Drs. George (Yuri) Fedoriw, John Hunt, and Stephanie Mathews.
SPECIAL COAGULATION LABORATORY
HERBERT C. WHINNA, M.D., Ph.D., DIRECTOR
The laboratory has completed a move to new space in the UNC Cancer Hospital to allow for
departmental growth in both our and other areas. We continue to perform special studies testing
for UNC researchers as well as pharmaceutical and equipment companies generating additional
revenue for UNCH with little increased cost/overhead. Faculty and staff regularly participate in
interdepartmental conferences, allowing us to optimize patient care and safety as well as plan for
the development and implementation of new testing in the future.
CYTOGENETICS
KATHLEEN W. RAO, Ph.D., DIRECTOR
KATHLEEN A. KAISER-ROGERS, Ph.D., CO-DIRECTOR
The caseload for the Cytogenetics Laboratory continued to grow in FY10-11 with increases seen
primarily in our oncology FISH case load. At the current time, the laboratory offers over 30
different interphase FISH assays, most of which are designed to diagnose or monitor specific
genetic abnormalities associated with cancer. Most recently we have validated the ALK break
apart assay for the identification of the ALK inversions and variant rearrangements that are seen
in some patients with non-small cell lung cancer.
On June 1st of 2010 the Cytogenetics laboratory replaced the 105,000 probe oligonucleotide
microarray with a high resolution 1.8 million probe whole genome SNP microarray for
evaluation of patients with developmental disabilities, dysmorphic features and congenital
anomalies. The SNP array enables us to detect not only copy number changes, but also long
continuous stretches of homozygosity associated with uniparental disomy (the inheritance of
both copies of a chromosome, or chromosome part, from a single parent) and consanguinity
(matings between closely related individuals), both of which can result in genetic disease. In
response to the American College of Medical Genetics practice guidelines published in
November of 2010 recommending microarray testing be performed as a first tier postnatal test
for patients developmental disabilities and/or congenital anomalies, the laboratory now offers the
option of a 5 cell or a 20 cell karyotype in conjunction with microarray testing.
We continue to characterize the chromosome rearrangements of some of our more interesting
patients using both traditional and molecular cytogenetic techniques including both fluorescence
in situ hybridization (FISH) and array CGH. The rearrangements and corresponding phenotypes
observed in four of our patients were reported in poster form at the March 2011 American
College of Medical Genetics Meeting.
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The Cytogenetics Laboratory continues to actively participate in the cancer cooperative groups
(CALGB and COG). During the past year, the UNC Cytogenetics Laboratory was a top
performer among the Children’s Oncology Group approved laboratories, with two consecutive 6
month periods of 100% abnormality detection and submission acceptance. Dr. Rao continues as
a member of the CALGB (Cancer and Leukemia Group B) Cytogenetics Committee and was
recently elected Chair of the Cytogenetics Committee for the Children’s Oncology Group (term
to begin July 1, 2011). She also continues in her roles as a member of the Board of Directors of
the American College of Medical Genetics and of the ISCN (International Standing Committee
for Cytogenetic Nomenclature).
LABORATORY INFORMATION SERVICES
HERBERT C. WHINNA, M.D., Ph.D., DIRECTOR
Major Projects from the past fiscal year for LIS included ACC lab interface implementation, Rex
Hospital HPV interface, several new instrument interfaces and auto-verification implementation
for the Coag and Hematology areas in Core Lab. The Softlab upgrade is nearing completion and
CoPath was upgraded to version 3.2 in preparation for Advanced Barcoding & Tracking
(AB&T). Synoptic Reporting implementation was completed for CoPath, with synoptic
worksheet phase-in to begin after AB&T implementation is completed.
Upcoming projects include CoPath AB&T (currently underway), Softlab migration to Oracle
database and Softlab hardware upgrade. We will also begin our partnership with RCM e-
Services for Outreach billing which will include implementation of SoftA/R, SoftWeb and
SoftExpress modules for the SoftLab system. Also scheduled is implementation of a computer
system for the HLA lab.
NEPHROPATHOLOGY LABORATORY
VOLKER R. NICKELEIT, M.D., DIRECTOR
The Division of Nephropathology in the Department of Pathology and Laboratory Medicine is
one of few highly specialized centers in the U.S. that provides expert diagnostic evaluation of
medical renal diseases and transplant related disorders. More than 1,700 renal specimens (native
& transplant biopsies and nephrectomies) from over 200 nephrologists throughout the state,
region and the world are analyzed annually. During the 2010 calendar year, the Division
evaluated close to 500 cases from UNC Hospitals, and the remainder from outside institutions.
Over 90% of specimens are routinely evaluated not only by light microscopy at multiple levels
of section with different stains, but also by immunofluorescence microscopy utilizing a panel of
antibodies, electron microscopy, and occasionally additionally by immunohistochemistry. Thus,
the actual number of procedures that are performed on renal specimens by far exceeds 5000 per
year. The Division of Nephropathology is involved in clinical, translational and basic research on
renal diseases, especially glomerulonephritides and diseases seen in renal allografts. The
research activities are supported by extramural grants and are facilitated by an extensive database
and archival system that currently includes data from approximately 30,000 renal specimens,
15,000 serum samples, and 1000 urine samples. Currently, one US pathologist and one
pathology post doctoral research associate from Sudan are being trained on how to manage and
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organize a nephropathology laboratory. The UNC nephropathology faculty is also heavily
engaged in continuous education series enhancing the diagnostic skills of pathologists and
nephrologists, such as short courses at the annual USCAP meetings, the Columbia Presbyterian
post graduate course on nephropathology in New York, or the 'Nephropathologiekurs Volhard-
Fahr' in Mannheim, Germany. The Division of Nephropathology is sponsoring and co-
organizing the upcoming 15th
International Vasculitis & ANCA Workshop in Chapel Hill. It is
closely allied with the UNC Kidney Center and the Glomerular Disease Collaborative Network
(GDCN). The GDCN has been in operation for over two decades and is a consortium of
academic and community nephrologists; it has the goal to enhance knowledge of renal diseases
and treatment strategies.
QUALITY MANAGEMENT GROUP
HERBERT C. WHINNA, M.D., Ph.D., DIRECTOR
The Quality Management Group has supported several new laboratory initiatives this year
including the development and implementation of Vitamin D testing by Mass Spec, opiate
confirmations, the addition of HE4 tumor markers. In addition they continue their six sigma,
Lean and kaizen activities including the addition of 5 new green belts who have completed
training and one new black belt. In addition, all employees in the area have completed yellow
belt training. This has equipped the area to complete a green belt project that is saving the
hospital $15,000 per month in previously lost revenue, a kaizen event in the Molecular area that
has improved work flow and space utilization, a green belt project in Core Laboratory to refine
the work flow in the processing area. Members of the group have also participated on a hospital
wide committee to evaluate and purchase new software for accessing MSDS information. The
new product will benefit and improve information for the entire hospital.
CLINICAL NEUROPATHOLOGY SERVICE AT UNC HOSPITALS
THOMAS W. BOULDIN, M.D., DIRECTOR
Diagnostic services in neuropathology are provided at UNC Hospitals by C. Ryan Miller, MD,
PhD; Dimitri G. Trembath, MD, PhD; and Thomas W. Bouldin, MD. Dr. Bouldin is the director
of the Division of Neuropathology. Neuropathology services include diagnostic surgical
neuropathology, autopsy neuropathology, forensic neuropathology, nerve biopsy interpretation,
and ophthalmic pathology. The surgical neuropathology service and autopsy service provide
sufficient neuropathology specimens to allow the Department of Pathology and Laboratory
Medicine to provide a rich training experience in diagnostic neuropathology for the
Department’s residents in anatomical and clinical pathology. The volume of surgical
neuropathology cases has continued to increase and become more complex over the last five
years, due in part to the growth of the clinical neurosurgical service, the expansion of the Neuro-
Oncology programs at UNC Hospitals, and the opening of the North Carolina Cancer Hospital.
The Neuropathology faculty members attend and are active participants in the weekly Neuro-
Oncology Multidisciplinary Conference at UNC Hospitals. A complete listing of the clinical
conferences conducted by the neuropathology faculty is as follows:
Brain Cutting Conference (Autopsy Service) Weekly
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Muscle and Nerve Biopsy Conference Weekly
Clinical Neurosciences Conference Monthly
Ophthalmic Pathology Signout Conference Weekly
Multidisciplinary Neuro-oncology Conference Weekly
OUTREACH LABORATORY SERVICES
HERBERT C. WHINNA, M.D., Ph.D., DIRECTOR
McLendon Laboratory’s Outreach Service operates as the primary interface between the
diagnostic testing services of the hospital laboratory and community. The service has grown to
serve over 70 clients in the research triangle area including hospitals, UNCH hospital clinics,
Triangle Physician Network practices, UNCH P&A clinics, skilled nursing facilities, private
physician practices and home health agencies. Twenty-six of the serviced providers perform
some level of point of care testing (from waived to moderately complex) and four of the sites are
CAP accredited. Last year Outreach continued serving over 100,000 patients and processed over
570,000 tests.
Outreach continued with the implementation of the administratively approved business plan.
Four vehicles were obtained through the Medical Foundation. Five couriers were hired (four
full-time and one part time), routes developed and MCL begin performing pickups in December
of 2010 expanding to the current service level of all routine pickups. Additional leadership
positions were filled to provide expanded management support (Supervisor) and business
development (Physician Liaison). A planned call center staffed with four employees that was
started. The call center centralizes incoming calls to core laboratory, Microbiology and Outreach
and is open from 7 am – 8 pm, M-F.
Outreach completed its Green Belt project focusing on one physician’s ordering/coding practices
and in working with that physician dramatically reducing write-offs. Based on this experience
Outreach will begin to expand these learned best practices to other providers and clinics. The
ambulatory care center laboratory opened in April of 2011 and provides an extended menu of
tests to support the operating room expansion. The moderately complex laboratory located at
Carolina Point 2 expanded its test menu to include chemistry, urinalysis and additional point of
care tests to support the Urgent Care clinic that opened May of 2011. Planning is continuing for
a physician office building in Hillsborough. The facility will have laboratory support for both
the clinics and an urgent care clinic.
TRANSPLANT LABORATORIES (HLA and Flow Cytometry)
JOHN L. SCHMITZ, Ph.D., DIRECTOR
The HLA and Flow Cytometry (FCL) Laboratories have undertaken steps to enhance the
efficiency of laboratory operations to enhance service to our clinicians and patients with
continued maintenance of our teaching mission.
The FCL completed validations and has implemented an in house developed test for oxidative
burst for use in the diagnosis of Chronic Granulomatous Disease as well as for Paroxysmal
Nocturnal Hemoglobinuria. The implementation of these tests has improved patient care by
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providing more robust and timely analyses. The FCL, working with Dr. Dunphy, is also
beginning validations of 6-color flow analysis for immunophenotyping of hematologic
malignancies. The anticipated implementation in the next fiscal year will enhance capabilities of
analyzing samples with limited cellularity as well a providing a more detailed analysis of cellular
phenotypes.
The HLA laboratory has taken several steps to enhance efficiency, customer service and quality
of laboratory testing activities. The laboratory has contracted with an outside organization (Path-
Tech) that provides sample collection kits (blood and buccal cell) to transplant donors around the
country for shipment to the laboratory for HLA testing. Transplant coordinators can now order
these kits to be sent to individuals via an online interface instead of working through laboratory
staff. This service provides a previously unavailable tracking mechanism for follow up on
samples that have not been received in the expected time frame, improves timeliness of sample
acquisition and saves laboratory technologist time. Along with this improvement, the laboratory
has implemented a modification to its buccal cell extraction protocol to improve the rate of
successful collections from 70 to 85% in terms of DNA concentration. In addition, the
laboratory has completed validations of a saliva collection kit that will replace the buccal kits in
the next fiscal year. The rate of recovery of adequate DNA is improved with these kits. The
laboratory has evaluated a new technology for HLA typing of deceased donors using real time
PCR. This method will be implemented in the next fiscal year to replace the current SSP method
because of the significantly shorter run time of the real time assay which will shorten the number
of hours needed for on call HLA typing services. Finally, the laboratory is working with the
Bone Marrow Transplant Service to implement a database program, Transchart, for reporting
HLA typing results. This program, which will interface with the Soft LIS, will improve data
reporting for BMT patients and provide a computerized final report mechanism to replace the
current manually generated BMT reports by the laboratory.
Finally, the Transplant Laboratories continue their extensive training and educational mission
within the Hospital. The FCL and HLA laboratories provided rotations for CLS students, and the
Immunology, Allergy/Immunology and Molecular Pathology Fellows. In addition, transplant
coordinators receive didactic training on laboratory technologies as do TMS, Nephrology and
Surgery Fellows. Finally, the laboratories provided educational services to a large number of
Medical Students in the PATY417 elective.
MUSCLE PATHOLOGY LABORATORY
LEIGH B. THORNE, M.D., DIRECTOR
For several years, this core provided processing and histochemical staining for frozen muscle
biopsies received in the UNCH Dept of Surgical Pathology. Approximately 80-100 muscle
biopsies were performed each year at UNCH. Beginning July 2010, the muscle pathology
services were transferred from the Pathology department to McLendon Clinical Laboratories.
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HUMAN PROGENITOR CELL LABORATORY
YARA A. PARK, M.D., DIRECTOR
Due to rapid growth of the Bone Marrow Transplant program, the Hematopoietic Progenitor Cell
lab processed a record number of products this year. The lab was inspected and re-accredited by
CAP, AABB, FDA, and FACT within the past year. Renovations are almost complete on an
expanded work area which will house the liquid nitrogen freezers. In conjunction with the Bone
Marrow Transplant program, the lab developed a new process and form to ensure the correct and
prompt determination of allogeneic donor eligibility by both the clinical program and the
laboratory.
CORE AND SERVICE LABORATORIES
MICROSCOPY SERVICES LABORATORY
C. ROBERT BAGNELL, Jr., Ph.D., DIRECTOR
The Bagnell laboratory - Microscopy Services Laboratory - is a UNC core facility for electron
and light microscopy. The laboratory is also the Light Microscopy Core facility for the
Lineberger Comprehensive Cancer Center. Additionally, it provides clinical electron microscopy
services. During this reporting period the laboratory supported research by 341 principal
investigators from 42 departments centers and programs at UNC-CH, 2 departments at Duke, 1
department at UNC-G, 1 investigator form NIEHS, and 1 investigator from Urogenix Inc. The
total number of active laboratory clients now stands at 957. In the past 12 months the light
microscope facilities logged 11,033 hours of use, electron microscope facilities logged 2,048
hours of use and the laboratory has performed 533 electron microscopy specimen preparations.
In addition to its research role, the laboratory serves as the primary electron microscope facility
for ultrastructural clinical diagnosis for UNC Hospitals and for Dr. Charles Jennette’s renal
pathology referral service. The laboratory also serves as an alternate for UNC Hospitals clinical
electron microscopy specimen preparation service. An NIH Shared Instrumentation Grant for a
new transmission electron microscope was re-submitted with a requested amount of $422,000.
This grant was not funded. The laboratory received $100,000 in funding from the Department of
Pathology & Lab Med. to purchase an Andor FRAP system for the Olympus IX81 microscope.
The laboratory received $3,000 in funding form the Department of Pathology & Lab Med to
purchase two TMC vibration isolation tables.
Checklist of significant projects during this reporting period:
Added Klaus Haun’s Biosensor imaging technology to the lab services
Added EDAX XES to FESEM
Added Andor FRAP system to IX81
Created ImageJ analysis program for Biosensor FRET analysis
Created ImageJ analysis program for fibrin orientation and anisotropy
Created ImageJ plugins to streamline sensitized emission wide field FRET analysis
Presented poster of lab services at UNC Core Lab Day
Presented photographs by clients at CHANL scientific Photo Contest
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Acknowledged (Steve Ray) in Cell Metabolism, Vol 13, Issue 5, pg. 517-526 “The
Microbiome and Butyrate Regulate Energy Metabolism and Autophagy in the
Mammalian Color” Scott Bultman Lab
Image analysis project Colocalization of synaptic markers (Cendra Agulhon Ken
McCarthy Lab)
Image analysis project Cell tracking from cardiac explants (Panna Tandon – Conlon Lab)
Image analysis project Measurement of FISH labeling and proximity to methyl-histone
(Mauro Calabrese – Teryy Magnuson Lab)
Image analysis project Analysis of AAV distribution in infected cells (PH Xiao – Richard
Samulski Lab)
Image analysis project Visualizing particle internalization by cells (Chris Luft – Joe
DeSimone Lab)
Image analysis project Analysis of drug localization in kidney (Rachel Goldsmith – Rick
Tidwell Lab)
Image analysis project Cell tracking in scratch assays and culture (Zhigang Zhou – Joan
Taylor Lab)
LASER CAPTURE MICRODISSECTION CORE FACILITY
C. ROBERT BAGNELL, Jr., Ph.D., DIRECTOR
This facility is part of the Microscopy Services Laboratory. LCM is a method for collecting very
small regions of tissue or specific cells for use in “omic” analyses. The facility houses a Zeiss
PALM LCM and an Arcturus PIX-Cell II LCM, a Leica CM 1850 cryostat, and a ventilation
hood for staining and dehydration. Over the past 12 months, the LCM systems were utilized by
eight investigators (Joan Taylor, John Wright, Yi Zhang, Lesley Marson (now with Urogenicx),
David Clemmons, Silviana Barros, Kristina Abel, and Pam Groben) for a total of 254 hours.
TRANSLATIONAL PATHOLOGY LABORATORY (TPL)
C. RYAN MILLER, M.D., Ph.D., FACULTY DIRECTOR
The Translational Pathology Laboratory (TPL) continues to grow, both in terms of services
offered and the volume of services provided to UNC investigators. We have acquired several
new pieces of equipment and analysis software packages, including a CRi/Caliper Life Sciences
Nuance multi-spectral imaging camera and associated inForm analysis software, the Definiens
XD Developer and Tissue Studio image analysis software, and upgraded Aperio Spectrum image
and data management software available at https://tpl-spectrum.med.unc.edu. In calendar year
2010, we provided services to 63 investigators (up from 52 in 2009) and 18 clinical trials.
Diagnostic slides and FFPE tissue blocks were pulled from the UNCH Surgical Pathology
archives on 939 patient cases. Over 13,000 unstained tissue sections, 2,400 H&E stained slides,
and 4,400 IHC/IF stained slides were prepared. Over 15,000 slides were scanned on the Aperio
ScanScope XT and ScanScope FL systems and over 3,700 quantitative slide analyses have been
performed. Our services have been included in over 20 grant applications and 12 peer-reviewed
publications.
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ANIMAL CLINICAL LABORATORY FACILITY
HYUNG-SUK KIM, Ph.D., DIRECTOR
The facility performs blood chemistry tests, urinalysis and hematological tests in animal samples,
to characterize physiological and clinical phenotypes in animal models. For clinical tests, 44
different chemicals including general health tests, liver function tests and kidney function tests
are currently available with an automated chemical analyzer, Ortho-Clinical Diagnostics Johnson
& Johnson’s VT350 (purchased in 2008), which can measure one test with 5 - 10 microliter
sample volume. For hematological tests, the animal blood counter (HESKA’s CBC Diff,
Veterinary Hematology System) can measure WBC#, Lym%, Lym#, Mon%, Mon#, Gra%,
Gra#, RBC#, HGB, HCT, MCV, MCH, MCHC, RDW, PLT, MPV, and 3 distribution curves of
WBC, RBC, and PLT with 20l whole blood sample. Since we have various data accumulated for
long period from normal or abnormal values, discussion with us will help to interpret clinical
results. More than thirty principal investigators from the UNC-CH campus use these services for
their research. From June 2011, the facility will provide the new service of multiplexed
biomarker immunoassays for cancer biomarkers, cell signaling phosphoprotein detection,
endocrine diseases, cardiovascular diseases, cytokine/chemokine detection. Recently, the new
MAGPIX instrument having development of Luminex technology with magnetic beads has been
purchased.
GENE EXPRESSION FACILITY
HYUNG-SUK KIM, Ph.D., DIRECTOR
The facility provides services for gene expression studies using quantitative real time RT-PCR
by ABI 7500 and 7300 Sequence Detection Systems and high throughput preparation of total
RNA and genomic DNA by ABI Prism 6100. Currently, more than 1,000 disease-related gene
assays have been developed to detect their expression levels in mice, human, and rat, including
various house-keeping genes. In addition, a service for mouse genotyping analysis has been well
established with a high throughput performance based on detecting differences of gene copy
number, with a less than two-day turn-around time. This genotyping process can exclude many
laborious procedures, such as preparation of genomic DNA, PCR, gel running, Southern blot
analysis. Currently, we are genotyping more than three thousand mice monthly. We can also
provide a full service which includes all the steps necessary for designing and synthesizing
Taqman probes and primers, preparing RNA samples, and quantitative analysis. Through full
service, we are collaborating with many PIs for gene expression research. More than thirty
principal investigators from ten different departments are currently using this research core
facility.
DNA SYNTHESIZING FACILITY
HYUNG-SUK KIM, Ph.D., DIRECTOR
The facility serves more than 50 investigators from a variety of campus-wide departments in its
function of producing oligonucleotides for use in genetic research. Three DNA Synthesizers can
produce ten oligonucleotides simultaneously. During this fiscal year, about three thousand
oligonucleotides have been synthesized. The fluorescent oligonucleotide TaqMan probes with 5’
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fluorescein (6-FAM) and 3’ quencher tetramethyl rhodamine (TAMRA) are successfully
prepared for users of real time RT-PCR.
ADME MASS SPECTROMETRY CENTER
ARLENE S. BRIDGES, Ph.D., DIRECTOR
RICHARD R. TIDWELL, Ph.D., CHAIR, ADVISORY BOARD
As Director of the ADME Mass Spectrometry Center, Dr. Bridges’ role is to provide
study design assistance, bioanalytical support, and date interpretation to preclinical and
clinical studies conducted by investigators at UNC and beyond. Center capabilities
include quantitation by triple quadrupole mass spectrometry, molecular weight
determination by ion trap mass spectrometry, and identification of novel metabolites by
both types of equipment.
With regards the equipment, the Center maintains:
1. an Applied Biosystems API4000 triple quadrupole mass spectrometer
2. an Applied Biosystems API3000 triple quadrupole mass spectrometer
3. a Thermo-Scientific Quantum Ultra triple quadruple mass spectrometer
4. an Agilent 1100 MSD ion-trap mass spectrometer
5. five Agilent HPLCs, each with diode array (UV) and fluorescence detectors
The Center supports the work of UNC principal investigators in the Schools of Medicine,
Pharmacy, and Public Health and in the College of Arts and Sciences. Primary research
activities involve analysis of antiparasitic agents (in collaboration with Dr. Richard
Tidwell), antibiotic and antiviral agents (in collaboration with Dr. Angela Kashuba), and
anticancer nanoparticles (in collaboration with Drs. Joseph DeSimone and William
Zamboni). In addition, the Center has collaborates long-distance with researchers from
Duke University, East Carolina University, North Carolina Central University, and the
University of Puerto Rico. Work conducted by the Center varies from simultaneously
quantifying seventeen different antiretrovirals in human plasma/semen/breast
milk/cervicovaginal fluid, to determining the kinetics of enzymatic reactions, to
identifying novel metabolites in complex biological matrices, to determining novel
bioactive compounds in dietary and herbal mixtures. Overall, users log over 200,000
hours of instrument time annually.
Over 30 students, post-doctoral fellows and faculty from across the UNC campus
have been trained in the safe and effective use of the analytical equipment in the Center.
More than 50 peer-reviewed publications have been written using data generated in the
Center. Dr. Bridges is waiting for the results of an NIH Shared Instrumentation Grant
that, if successful, will enable the Center to purchase a much needed quantitative, high resolution
mass spectrometer.
FACULTY AND SENIOR STAFF CHANGES
ARABA AFENI-ANNAN, M.D., was on leave December 8, 2010 through June 30, 2011.
74
CLAUDIA M. BRADY, M.H.S., was promoted to Assistant Professor effective May 1, 2011.
MEGAN J. DIFURIO, M.D., was appointed Assistant Professor effective August 18, 2010.
She serves as attending pathologist and focuses on diagnostic pathology, cytopathology and
surgical pathology.
DAVID A. EBERHARD, M.D., Ph.D., was appointed Associate Professor and Member,
Lineberger Comprehensive Cancer Center effective April 15, 2011. Using pathology and genetic
techniques, he performs translational research studies for transfer of research from the bench to
the bedside.
THOMAS H. FISCHER, Ph.D., changed to Adjunct Associate Professor effective March 12,
2011.
ADIL HUSSEIN GASIM, M.D., was promoted to Assistant Professor effective June 1, 2011.
He participates in pathologic and clinical research on kidney diseases.
PETER H. GILLIGAN, Ph.D., changed his primary academic appointment to the Department
of Pathology and Laboratory Medicine effective April 1, 2011. He holds a joint appointment
(secondary) as Professor of Microbiology and Immunology.
KEVIN E. GREENE, M.D., was appointed Assistant Professor effective July 1, 2010. He
serves as attending pathologist in surgical pathology and focuses on gastrointestinal and liver
pathology.
PAMELA A. GROBEN, M.D., was appointed Professor, 25% FTE, effective July 1, 2010, to
serve as attending pathologist in surgical pathology and provide pathologic interpretation of skin
samples as a board certified dermatopathologist. She will also collaborate in research studies.
J. EDWIN HALL, Ph.D., retired from State service on December 31, 2010.
STEVEN HOLMES, B.S., M.H.S., was appointed Assistant Professor effective July 1, 2010.
He serves as Pathologists’ Assistant.
HEIKE HUNT, M.D., was on leave July 1, 2010 through September 26, 2010 and January 1,
2011 through June 30, 2011.
JOHN HUNT, M.D., was on leave September 28, 2010 through October 18, 2010
KATHLEEN KAISER-ROGERS, Ph.D., was promoted to Professor effective May 1, 2011.
75
APRIL E. KEMPER, M.S., M.H.S., was appointed Assistant Professor effective July 1, 2010.
She serves as Pathologists’ Assistant.
SARA KOENIG, M.D., resigned December 2010, to accept a position at the University of New
Mexico.
ROMMEL P. LU, M.D., was appointed Assistant Professor effective July 8, 2011. He serves as
attending pathologist in Transfusion Medicine.
STEPHANIE P. MATHEWS, M.D., was appointed Assistant Professor, 20% FTE, effective
November 1, 2010. She serves as attending pathologist in hematopatology.
CHRISTOPHER R. MCCUDDEN, Ph.D., resigned his position effective June 29, 2011 to
return to his home country, Canada.
CLAY NICHOLS, M.D., was appointed Professor effective July 1, 2011. He is Deputy Chief
Medical Examiner, Office of the Chief Medical Examiner for the State of North Carolina.
SIOBHAN M. O’CONNOR, M.D., was appointed Assistant Professor effective July 1, 2010.
She serves as attending pathologist in surgical pathologist and cytopathology with a focus on
breast pathology.
KUMAR R. PANDYA, Ph.D., was appointed Assistant Professor effective January 1, 2011 for
collaborative research with Oliver Smithies, D.Phil.
LORI R. SCANGA, MD., Ph.D., was appointed Assistant Professor effective July 1, 2010. She
serves as attending pathologist in surgical pathology and cytopathology with focus on
gynecologic diseases.
JOHN SCHMITZ, Ph.D., was promoted to Professor effective August 1, 2010.
HARSHARAN K. SINGH, M.D., was promoted to Professor effective June 30, 2011.
LEIGH B. THORNE, M.D., was promoted to Associate Professor effective May 1, 2011.
RUTH F. WALTERS, M.D., was appointed Adjunct Assistant Professor effective July 1, 2011.
KAREN E. WECK, M.D., was promoted to Professor effective July 1, 2010.
ALISA S. WOLBERG, Ph.D., was promoted to Associate Professor effective July 1, 2011.
MAIMOONA B. ZARIWALA, Ph.D., was promoted to Associate Professor effective February
1, 2011.
76
SPECIAL HONORS AND AWARDS
WILLIAM B. COLEMAN, Ph.D.
2011 Blue Faery Award for Excellence in Liver Cancer Research from Blue Faery – The
Adrienne Wilson Liver Cancer Association, April 2011
MARILA CORDEIRO-STONE, Ph.D.
Dr. Cordeiro-Stone was asked by two graduates, one from the Curriculum in Toxicology and
another from the Department of Biochemistry and Biophysics, to participate in hooding them at
the May 7th
2011 Commencement Ceremony.
SUSAN C. HADLER, M.D., M.S.
Sophomore Basic Sciences Teaching Award, Awarded by the UNC Medical Class of 2013
2011 White Coat Ceremony UNC Medical School: Coater
CATHERINE A. HAMMETT-STABLER, Ph.D.
AACC Outstanding Speaker Award, 2010
CRAIG A. FLETCHER, D.V.M., Ph.D.
Dr. Fletcher was accepted into the Office of Human Resources, University Leadership Education
and Development (ULEAD) program that provides an opportunity to develop highly skilled and
motivated leaders prepared to meet the challenges of the University’s changing environment.
The program’s goals include: (1) increasing awareness of your professional strengths and
developmental needs through assessment and coaching, (2) expanding the knowledge, skills, and
abilities critical to those in a changing University environment through a range of experiential
and classroom sessions.
JUDITH NIELSEN, D.V.M.
2010 Citation in Faculty of 1000 Biology: evaluations for Okagaki LH, Strain AK, Nielsen JN,
Charlier C, Baltes NJ, Chretien F, Heitman J, Dromer F, Nielsen K, (2010) Cryptococcal Cell
Morphology Affects Host Cell Interactions and Pathogenicity. PLoS Pathog 6 (6): e1000953.
Doi:10.1371/journal.ppat.1000953: e1000953
http://f1000biology.com/article/id/3866963/evaluation
KUMAR R. PANDYA, Ph.D.
Dr. Pandya received the 2010 Commitment to Service Award for Unique Contributions in the
areas of mentoring, service, or leadership – UNC Office of Post Doctoral Affairs.
77
JOAN M. TAYLOR, Ph.D.
2011 Star Heel Award
ELECTED LEADERSHIP POSITIONS
WILLIAM B. COLEMAN, Ph.D.
Secretary-Treasurer, The American Society for Investigative Pathology, July 2007-Present
CHERIE H. DUNPHY, M.D.
President, North Carolina Pathology Society (through April 9, 2011)
WILLIAM K. FUNKHOUSER, M.D., Ph.D.
Council Member, Associate Director Anatomy Surgical Pathology (ADASP)
PETER H. GILLIGAN, Ph.D.
Dean, American College of Microbiology
Board of Goverors, American Academy of Microbiology
CATHERINE A. HAMMETT-STABLER, Ph.D.
President, Americal Association of Clinical Chemistry
President, National Registry of Certified Chemists
J. CHARLES JENNETTE, M.D.
Past-President, Association of Pathology Chairs
HARVEY MICHAEL JONES, M.D.
Board of Governors, American Osler Society
SUSAN T. LORD, Ph.D.
Board of Councilors, International Fibrinogen Research Society
CHRISTOPHER P. McCUDDEN, Ph.D.
Executive Committee: North Carolina Section of the American Association for Clinical
Chemistry
78
JUDITH NIELSEN, D.V.M.
North Carolina Academy of Laboratory Animal Medicine, President-Elect 2 yrs.
VOLKER R. NICKELEIT, M.D.
Councilor, Executive Committee, Renal Pathology Society
KATHLEEN W. RAO, Ph.D.
Executive Committee, International Standing Committee and Human Cytogenetic Nomenclature
Executive Committee, Board of Directors, American College of Medical Genetics (ACMG)
HOWARD M. REISNER, Ph.D.
Councilor, UMED
JOHN L. SCHMITZ, Ph.D.
President, Association of Medical Laboratory Immunologists
HARSHARAN K. SINGH, M.D.
Vice Secretary, Renal Pathology Society, January-December 2010
Secretary, Renal Pathology Society, January 2011 – June 30, 2011
KAREN E. WECK, M.D.
Chair, Training and Education Committee, Association for Molecular Pathology
JULIA W. WHITAKER, D.V.M.
Board of Directors, North Carolina Academy of Laboratory Animal Medicine
RUTH E. WINECKER, Ph.D.
Chair, AAFS, Toxicology Section Program Chair
Secretary, AAFS, Toxicology Section Secretary
Board of Directors, ABFT, Board of Directors
JOHN T. WOOSLEY, M.D., Ph.D.
Member at Large, Association of Pathology Chairs
79
LEADERSHIP POSITIONS
JOHN F. CHAPMAN, Dr.P.H.
Chair, CCT Committee, NRCC
Member (Advisor), CLSI Subcommittee on Serem Indices
WILLIAM B. COLEMAN, Ph.D.
Council, The American Society for Investigative Pathology
Finance Committee Chair, The American Society for Investigative Pathology
Finance Committee, Federation of American Societies for Experimental Biology
Publications Committee, The American Society for Investigative Pathology
Divisional Oversight Committee, The American Society for Investigative Pathology
Membership Committee, The American Society for Investigative Pathology
Education Committee, The American Society for Investigative Pathology
MARILA CORDEIRO-STONE, Ph.D.
Member, Society of Toxicology, Career Resource and Development (CRAD) Committee (May1,
2009 to April 30, 2012)
Interim Director, Curriculum in Toxicology
Director of Graduate Studies, Curriculum in Toxicology
CHERIE H. DUNPHY, M.D.
Chair, College of American Pathologists Diagnostic Immunology Resource Committee
Chair, College of American Pathologists Hematology/Hematopathology Competency Dictionary
Page Working Group, August, 2010-present
ROSANN A. FARBER, Ph.D.
Judge, Annual DNA Day Essays, American Society for Human Genetics
CRAIG A. FLETCHER, D.V.M., Ph.D.
Co-Chair, International Mock Board Exam Coalition for the American College of Laboratory
Animal Medicine Board Exam, 2010
Interim Associate Director, Division of Laboratory Animal Medicine:
Senior Clinical Veterinarian
MARGARET L. GULLEY, M.D.
Chair, Topics Committee, Evaluation of Genomic Applications in Practice and Prevention
(EGAPP) Stakeholder’s Group
80
Chair, Epithelial Cell Section, 14th
International Symposium on Epstein-Barr virus Associated
Diseases, Birmingham, UK September 2010
TRACY HEENAN, D.V.M.
Council Member, Certification of Professional IACUC Administrators (CPIA)
Chair, CCPIA Leadership Committee
Chair, CCPIA Exam Item Review
Ad hoc consultant. Association for the Assessment and Accreditation for Laboratory Animal
Care International (AAALAC)
JONATHON W. HOMEISTER, M.D., Ph.D.
Section Chair, 13th
Biennial Medwest Platelet Conference. Inflammation and Cardiovascular
Disease.
Session Chair, American Society for Investigative Pathology, Symposium Chair
J. CHARLES JENNETTE, M.D.
Chair, APC Nominating Committee
Session Chair, International Pediatric Nephrology Association Renal Pathology Pre-Course for
Pediatric Nephrologists, “Nephritic Syndrome”, New York, NY, August 2010
Session Chair, Renal Pathology Society Satellite Meeting ar the XXVIII International Congress
of the International Academy of Pathology, “Infectious Kedney Diseases”, Sao Paulo, Brazil,
October 2010
HARVEY MICHAEL JONES, M.D.
Chair, American Osler Society, Program Committee
Chair, American Osler Society, Publications Committee
KATHLEEN A. KAISER-ROGERS, Ph.D.
Chair, American College of Medical Genetics Salary Survey Committee
DAVID G. KAUFMAN, M.D., Ph.D.
Chair, Society of Toxicology, Scientific Liaison Task Force
SUSAN T. LORD, Ph.D.
Chair, International Society of Thrombosis and Haemostasis, Scientific Program Organizing
Committee for XXII Congress
81
CHRISTOPHER McCUDDEN, Ph.D.
Round Table Chair, “Career Perspectives for Young Scientists”. Journee Internationales de
Biologie, Paris, France, November 2010
Chair, Society for Young Clinical Laboratorians, American Asociation for Clinical Chemistry
MELISSA B. MILLER, Ph.D.
Session Chair, 50th
Interscience Conference on Antimicrobial Agents and Chemotherapy, “Best
Practices in the Laboratory Diagnosis of Sexually-transmitted infections,” Interactive
Symposium, Boston, MA, September 2010
Session Chair, American Society for Microbiology, 111th
General Meeting, Core Curriculum,
May 23, 2011
C. RYAN MILLER, M.D., Ph.D.
Member, National Cancer Institute, The Cancer Genome Atlas (TCGA), Glioblastoma Disease
Working Group (DWG)
Member, Scientific Advisory Committee, National Functional Genomics Center
JUDITH NIELSEN, D.V.M.
American College of Laboratory Animal Medicine, Exam Review Committee, 3 yrs.
VOLKER R. NICKELEIT, M.D.
Session Chair, ASN 2010: Clinico-Pathologic Correlation Conference, November 2010
Banff- Group: Chair of Working Group / Task Force on “Polyomavirus Nephropathy
Classification”
ARLIN B. ROGERS, D.V.M., Ph.D.
Chair, ACVP, Annual Meeting Plenary Session
Co-Chair, ASIP, Liver Workshop
Faculty Director, Animal Histopathology Core
JOHN L. SCHMITZ, Ph.D.
Chair, Credentials Committee: American Board of Medical Laboratory Immunology
Co-Chair, ASHI Accreditation Review Board
JOAN M. TAYLOR, Ph.D.
Chair, AHA, Early Career Development Committee
82
RICHARD R. TIDWELL, Ph.D.
The Bill and Melinda Gates Foundation, Initiative on Public-Private Partnerships for Health
(IPPPH)
World Health Organization, Drug Development, Preclinical and Clinical Studies; Treatment and
Drug Resistance section for the World Health Organizations Scientific Working Group on
African Trypanosomiasis Product Development Partnerships for Neglected Global Health
Medicines for Malaria Ventures, Expert Scientific Advisory Committee
HERBERT C. WHINNA, M.D., Ph.D.
Chairman, Scientific Subcommittee on Plasma Coagulation Inhibitors of the International
Society for Thrombosis and Hemostasis
JULIA W. WHITAKER, M.S., D.V.M.
Co-Chair, International Mock Board Exam Coalition for the American College of Laboratory
Animal Medicine Board exam review
MONTE S. WILLIS, M.D., Ph.D.
Chair, Nomination Committee, National Sigma Xi Research Society
Chair, Program Committee for Experimental Biology, American Society of Investigative
Pathologists
MEMBER OF BOARD OF DIRECTORS OF NATIONAL/INTERNATIONAL
ACCREDITATION AGENCY
JOHN F. CHAPMAN, Dr.P.H.
Member, National Registry of Certified Chemists
GEORGETTE A. DENT, M.D.
Member, Liaison Committee on Medical Education
CATHERINE A. HAMMETT-STABLER, Ph.D.
Member, BOD, National Registry of Certified Chemists
83
MELISSA B. MILLER, Ph.D.
Member, Board of Governors, American College of Microbiology
JOHN L. SCHMITZ, Ph.D.
Member, American Society of Histocompatibility and Immunogenetics Accreditation Review
Board
Member, American Board of Medical Laboratory Immunology
MEMBER OF FDA, CDC OR COMPARABLE COMMITTEE
JOHN F. CHAPMAN, Jr., Dr.P.H.
Member, (Advisor) CLSI Subcommittee on Serum Indices
FRANK C. CHURCH, Ph.D.
Organizer for the 6th
International Meeting on Serpins, planned for October, 2011, UNC-CH
WILLIAM K. FUNKHOUSER, Jr., M.D., Ph.D.
Member, Medical Devices Advisory Committee
CHRISTOPHER R. McCUDDEN, Ph.D.
Member, Sub-Committee on Serum Indices in the Clinical Laboratory. Clinical Laboratory
Standards Institute (CLSI)
MELISSA B. MILLER, Ph.D.
Member, FDA Microbiology Devices Panel
VOLKER R. NICKELEIT, M.D.
Member: OPTN/UNOS Ad Hoc Disease Transmission Advisory Committee
KATHLEEN W. RAO, Ph.D.
Member, Children’s Oncology Group Cytogenetics Central Review Committee
Member, Children’s Oncology Group, Infant Leukemia Committee
Member, Cancer and Leukemia Group B (CALGB) Cytogenetics Review Committee
84
JOHN L. SCHMITZ, Ph.D.
Member, United Network for Organ Sharing (UNOS) Histocompatibility Committee
KAREN E. WECK, M.D.
Member, Molecular and Clinical Genetics Devices Panel Consultant, FDA Medical Devices
Advisory Committee
ALISA S. WOLBERG, Ph.D.
Member, FDA, Steering Committee, Workshop on IGIV and Thrombosis, “Risk Mitigation
Stratigies to Address Potential Procoagulant Activity in Immune Globulin Products”, 2011
MEMBER OF NIH OR COMPARABLE STUDY SECTION
ARABA N. AFENYI-ANNAN, M.D., M.P.H.
NHLBI Sickle Cell Disease Guidelines Expert Panel
FRANK C. CHURCH, Ph.D.
Member of American Heart Association, Council on Arteriosclerosis, Thrombosis and Vascular
Biology Council Leadership Committee, Mid-Atlantic Region (Vice-Chair, 2006; Chair 2007-
2010)
Clinical and Experimental Therapy Study Section 3, Department of Defense, Federal Breast
Cancer Idea Grants.
WILLAM B. COLEMAN, Ph.D.
ad hoc External Grant Reviewer for the National Cancer Institute, National Institutes of Health,
NCI-F Manpower and Training Study Section, September 2010
ad hoc External Grant Reviewer for the National Institutes of Health, Cancer Diagnostics and
Treatment SBIR/STTR Study Section, October 2010
ad hoc External Grant Reviewer for the National Institutes of Health, Cancer Prevention Special
Emphasis Panel, May 2011
ad hoc External Grant Reviewer for the National Institutes of Health, Clinical and Translational
Imaging Study Section, May 2011
ad hoc External Grant Reviewer for the National Institutes of Health, Cancer Diagnostics and
Treatment SBIR/STTR Study Section, June 2011
ad hoc External Grant Reviewer for the National Institutes of Health, Cancer Biology and
Therapeutics Study Section, June 2011
85
GEORGETTE A. DENT, M.D.
Member, American Society of Hematology Committee on Promoting Diversity
Member, External Reviewer for the Annual Meeting of the Association of American Medical
Colleges
CHERIE H. DUNPHY, M.D.
Active Pathologist Reviewer, Children’s Oncology Group
Active Pathologist Reviewer, Cancer and Leukemia Group B (CALGB)
DAVID EBERHARD, M.D., Ph.D.
NCI, Clinical Assay Development Program, 2011-2012
CRAIG A. FLETCHER, D.V.M., Ph.D.
Member, Special Emphasis Panel Ad Hoc (June 16-18, 2010)
WILLIAM K. FUNKHOUSER, M.D., Ph.D.
Member, UNC TRACS Study Section
DAVID G. KAUFMAN, M.D., Ph.D.
IMAT Study Section, National Cancer Institute, June 2010
JOE N. KORNEGAY, D.V.M., Ph.D.
Ad hoc Reviewer, NIH-NHEB1, K99/R00 NIH Pathway to Independence Applications
Member, Muscular Dystrophy Association, Medical Advisory Committee, Grant Review
Sessions, October 2010 and May 2011
Ad hoc Reviewer, Dutch Parent Project Muscular Dystrophy, April, 2010
SUSAN T. LORD, Ph.D.
Member, College of CSR Reviewers
CHRISTOPHER P. MACK, Ph.D.
Member, NHLBI, Minearlcorticoid receptor, role in cardiovascular disease
HOWARD M. REISNER, Ph.D.
Member, ITRS Final Review panel Louisiana Board of Regents
86
Scientific Auditor for CRDF/NSF grants in the Ukraine and Georgia. On site review/audit.
Reviewer, Twenty-first Century Fund, State of Indiana
ARLIN B. ROGERS, D.V.M., Ph.D.
NCRR, Special Emphasis Panel: Pathobiology of Emerging Pathogens in Laboratory Animals
RICHARD R. TIDWELL, Ph.D.
NIH, NIAID, ICTDR. Human African Trypanosomiasis: Strategic Research Direction
MONTE S. WILLIS, M.D., Ph.D.
Ad hoc Grant Reviewer, South African Medical Research Council
Ad hoc Grant Reviewer, The Wellcome Trust
BERNARD E. WEISSMAN, Ph.D.
Member, NIH/CSR, Cancer Genetics
ALISA S. WOLBERG, Ph.D.
Member, Amer Heart Assn, Thrombosis/Throm BSCT2,
Member, NIH, Special Emph Panel/SRG 2011/05 HLBP S
SERVICE AS EDITOR OR ON EDITORIAL BOARDS
FRANK C. CHURCH, Ph.D.
Editorial Board, J. Biol. Chem.
Editorial Board, J. Thromb. Haemost.
Editorial Board, Thrombosis
Editorial Board, Thrombosis Research, special review series
WILLIAM B. COLEMAN, Ph.D.
Associate Editor, BMC Cancer (M. Norton, Editor-in-Chief)
Editorial Board, Clinica Chimica Acta (C.W. Lam, Editor-in-Chief)
Editorial Board, The American Journal of Pathology (M.P. Lisanti, Editor-in-Chief)
Editorial Board, Experimental and Molecular Pathology (J.M. Cruse, Editor-in-Chief)
Editorial Board, Archives of Pathology and Laboratory Medicine (P.T. Cagle, Editor-in-Chief)
Editorial Board, Laboratory Investigation (G.P. Siegel, Editor-in-Chief)
87
GEORGETTE A. DENT, M.D.
Member, Editorial Advisory Committee, UNC Medical Bulletin
CHERIE H. DUNPHY, M.D.
Chief Editor, E-Medicine, Hematopathology Section, Pathology
Associate Editor, Archives of Pathology and Laboratory Medicine
Associate Editor, Haematologia
Associate Editor, Case Reports in Medicine
Associate Editor, International Journal of Medical and Biological Frontiers
GEORGE FEDORIW, M.D.
Editorial Board, e-Medicine Pathology
WILLIAM K. FUNKHOUSER, M.D., Ph.D.
Section Editor, Molecular Pathology, APLM
PETER H. GILLIGAN, Ph.D.
Editor, Journal of Clinical Microbiology/Clinical Microbiology Reviews
Associate Editor, MBio
Editorial Board, Diagnostic Microbiology and Infectious Diseases
Editorial Board, Journal of Pediatric Infectious Diseases
MARGARET L. GULLEY, M.D.
Editorial Board, American Journal of Surgical Pathology
Editorial Board, Diagnostic Molecular Pathology
Editorial Board, PLOS Currents: Evidence for Genomic Applications
CATHERINE A. HAMMETT-STABLER, Ph.D.
Associate Editor, Clinical Chemistry
JONATHON HOMEISTER, M.D.
Editorial Board, Journal of Molecular and Cellular Cardiology
HEIKE HUNT, M.D.
Editorial Board, Annals of Hepatology
Editorial Board, Digestive Diseases and Science
88
J. CHARLES JENNETTE, M.D.
Section Editor – Immunopathology, American Journal of Clinical Pathology
Section Editor – Pathology, Journal of Nephrology
Editorial Board, American Journal of Kidney Diseases
Editorial Board, Archives of Pathology and Laboratory Medicine
Editorial Board, Clinical and Diagnostic Laboratory Immunology
Editorial Board, Clinical Journal of the American Society of Nephrology
Editorial Board, Journal of Rheumatology
Editorial Board, Kidney International
Editorial Board, Laboratory Investigation
Editorial Board, Pathology Case Reviews
DAVID G. KAUFMAN, M.D.
Editorial Board, Experimental and Molecular Pathology
Editorial Board, Frontiers of Biosciences
Editorial Board, Translational OncoGenomics
Editorial Board, Clinical Medicine: Pathology
Editorial Board, The Open Reproductive Science Journal
CHRISTOPHER R. MCCUDDEN, Ph.D.
Editorial Board, Laboratory Medicine
MELISSA B. MILLER, Ph.D.
Editorial Board, Journal of Clinical Microbiology
Editorial Board, Diagnostic Microbiology and Infectious Disease
C. RYAN MILLER, M.D., Ph.D.
Editorial Board, Brain Pathology
Editorial Board, Brain Research Bulletin
JUDITH NIELSEN, D.V.M.
Ad hoc reviewer: JAALAS, January, 2011
VOLKER R. NICKELEIT, M.D.
Editorial Board, Kidney and Blood Pressure Research
Editorial Board, Nephrology Dialysis Transplantation Educational eTOC
Editorial Board, World Journal of Transplantation
89
ARLIN B. ROGERS, D.V.M, Ph.D.
Editorial Board, Veterinary Pathology
JOHN L. SCHMITZ, Ph.D.
Editorial Board, Clinical and Vaccine Immunology
Editorial Board, Journal of Immunologic Methods
JOAN M. TAYLOR, Ph.D.
Editorial Board, ISRN Cell Biology
DITMITRI G. TREMBATH, M.D., Ph.D.
Editorial Board, Journal of Neuropathology and Experimental Neurology
KAREN E. WECK, M.D.
Associate Editor, Genetics in Medicine
Associate Editor, Molecular Genetics and Pharmacogenomics
Editorial Board, Journal of Molecular Diagnostics
Editorial Board, Expert Review of Molecular Diagnostics
BERNARD E. WEISSMAN, Ph.D.
Member, Editorial Board, Journal of Cellular Physiology
Member, Honorary Editorial Board, Lung Cancer, Targets and Therapy
Member, Editorial Board, Genetics Research International
MONTE S. WILLIS, M.D., Ph.D.
Editorial Advisory Board, Assistant Editor, Laboratory Medicine
Editorial Board, American Journal of Physiology – Heart and Circulatory Physiology
Editorial Board, Journal of Molecular and Cellular Cardiology
Editorial Board, Skeletal Muscle
ALISA S. WOLBERG, Ph.D.
Associate Editor, Arteriosclerosis, Thrombosis, and Vascular Biology Special Issue on Venous
Thromboembolism: 2011
Editorial Board Member, Arteriosclerosis, Thrombosis, and Vascular Biology
Advisory Board Member, Journal of Thrombosis and Haemostasis
90
JOHN T. WOOSLEY, M.D., Ph.D.
Editorial Board, Human Pathology
XIANWEN YI, M.D., Ph.D.
Editorial Board, European Journal of Pharmacology
Editorial Board, The Journal of Nutrition
INVITED LECTURES AT STATE/NATIONAL AND INTERNATIONAL MEETINGS
WILLIAM B. COLEMAN, Ph.D.
COBRE Center for Cancer Research Development and Center for Stem Cell Biology
Symposium, September 2010, Rhode Island Hospital, Providence, RI
Oral Presentation: “Targeting the epigenome for improved treatment of basal-like breast
cancers.” W.B. Coleman (Presenter)
XXX National Congress of the Italian Society of Pathology, October 2010, University of
Salerno, Salerno, Italy
Oral Presentation: “Small hepatocyte-like progenitor cells in liver regeneration.” W.B. Coleman
(Presenter)
American Society for Investigative Pathology, Annual Meeting, April 2011, Washington, DC
Oral Presentation: “Hidden in plain sight: How biology reveals new ways to treat breast cancer.”
W.B. Coleman (Presenter)
MARILA CORDIERO-STONE, Ph.D.
Special scientific symposium in honor of Dr. Rogerio Meneghini at the University of São Paulo
(December 18, 2010, São Paulo, SP, Brazil): Responses to UV-induced DNA damage in human
S phase cells. Invited by Dr. Carlos F. M. Menck
GEORGETTE A. DENT, M.D.
“GSA Hot Topic Session: MSPE/LCME”, AAMC Annual Meeting, Washington, DC
“Go with the Flow: The Use of Flow Cytometry in Diagnostic Hematopathology”, presented at
the University of Missouri of Medicine, November 2010
CHERIE H. DUNPHY, M.D.
“HEMA, Applications of Flow Cytometry to Myeloid Neoplasms”, The University of Texas
Health Science Center, San Antonio, TX, July 2010
91
CRAIG A. FLETCHER, D.V.M., Ph.D.
The C.L. Davis, DVM Foundation and the North Carolina Association of Laboratory Animal
Medicine Workshop in Laboratory Animal Medicine, Thursday, May 13, 2010, North Carolina
State University, College of Veterinary Medicine, Raleigh, NC
PETER H. GILLIGAN, Ph.D.
Molecular Screening for MRSA: Impact on Patient Care, American Association of Clinical
Chemists, July, 2010
The Laboratory Perspection on the Value of In Vitro Synergy Testing ICAAC, Boston, MA,
September, 2010Update on Clinincal Microbiology, MAHEC with video-feed to Area L,
Greensboro, Eastern and Wake AHEC, Ashville, NC, September 2010
The Rode of the Laboratory in the Detection and control of Multi-Drug Resistant Staphylococcus
Aureus, Bioconference Live Videoconference, October 2010
So you THINK you know Microbiology? NYC ASM, New York, NY, November 2010
Infectious Disease Conferences x 3, Jan, Feb (fellows only), June
MARGARET L. GULLEY, M.D.
“Taking advantage of human and viral expression profiles in blood, biopsy, and gastric juices for
early diagnosis and management of gastric cancer”. NCI Gastric and Esophageal Cancee
Meeting. Bethesda, May 16, 2011.
"Molecular Surgical Pathology for the Practicing Pathologist", Educational Course (9 talks),
American Society for Clinical Pathology, San Francisco, April 13-15, 2011.
"Sarcoma” and “EBV Associated Disease”, Association for Molecular Pathology Board Review
Course, Bethesda, April 29-30, 2011.
“Molecular tools to diagnose, monitor and predict outcome in cancer patients: Applications to
Epstein-Barr virus-related malignancy”. Grand Rounds of the East Carolina University
Department of Pathology, March 14, 2011.
CATHERINE A. HAMMETT-STABLER, Ph.D.
Collaboration with the Pain Medicine Clinic, AACCF New Jersey Local Section, November
2010
The State of TDM: A Retrospective Review. AACC Southeast Local Section, November 2010
Osteoporosis in 2010/ Michigan Local Section, November 2010
Laboratory Medicine Support of Pain Management – Beyond Urine Drug Testing. Australasian
Association of Clinical Biochemistry and Australian Institute of Medicial Scientists; Perth,
Australia, October 2010
What’s in this Urine? Australasian Association of Clinincal Biochemists/Australian Institute of
Medical Scientists; Perth, AU, October 2010
Understanding the Needs of the Pain Clinic. AACC Ohio Valley Local Section, Indianapolis,
IN. October 2010
Enhancing Toxicology Support of Pain Management. AACC Capital Local Section, Baltimore,
MD, September 2010
92
Boning Up on the Use of Biological Markers in Osteoporosis. AACC North Carolina Local
Section. Chapel Hill, NC, September 2010
Support Your Local Pain Clinic – the Laboratory’s Role. AACC Pacific North West Local
Section. Portland, OR. August 2010
Therapeutic Drug Monitoring 2010: Where Do We Need to Go? Department of Laboratory
Medicine; Cliniques Universitaires St-Luc; Brussels, Belgium, Spetember 2010
Survivor: Plants vs the World. Kaiser Permanente NW Laboratory. Portland, OR. August 2010
So You Think You Know What Those Results Mean? Palliative and Supportive Care Journal
Club. June 2010
Opportunities and Challenges in Laboratory Support of a Pain Management Program.
Contemporary Issues in Clinical TDM/Tox. Chicago, IL. April 29, 2011
Was it a Poisoning? Toxicology in the Clinical Setting. The Curriculum in Toxicology Seminar
Series; January 31, 2011.
TRACY HEENAN, D.V.M.
Heenan, Tracy, Faculty Workshop A12 (double session), IACUC Administrators Share and
Compare Ideas and Processes (IACUC Administration/Management and Process Track), March
31, 2011, Public Responsibility in Medicine and Research (PRIM&R) IACUC Conference –
Chicago, IL
Heenan, Tracy, Faculty Workshop B18 (workshop), Training Programs for Personnel at Large
Institutions (Qualifications and Training Track), March 31, 2011; PRIM&R IACUC Conference
– Chicago, IL
JONATHON W. HOMEISTER, M.D., Ph.D.
“Applying for Peer Reviewed Funding” Career Development Workshop and Breakfast.
Transition to Principal Investigator. EB2011, Washington D.C., April 10, 2011
HEIKE HUNT, M.D.
“Pancreas Tumors”, Tufts University Medical Center, Baystate Medical Center, Springfield,
MA, October 2010
JOHN HUNT, M.D.
Unexpected presentations of hematolymphoid neoplasms in non-lymphoid surgical specimens,
Thursday, March 10, 2011; Dept of Pathology (St. Michael’s Hospital, Toronto, Ontario)
J. CHARLES JENNETTE, M.D.
American Society of Nephrology Renal Week Postgraduate Education Course: Basic Renal
Pathology – from Bedside to Bench, “IgA Nephropathy”. “Diabetic Glomerulosclerisis”,
“Crescentric Glomerulonephritis”, and “Vasculitis”, Denver, CO, November 2010
93
American Society of Nephrology Renal Beek Postgraduate Education Course:
Glomerulonephritis Update: “Pathology of Rapidly Progressive Glomerulonephritis” and
“Pathology and Classification of Lupus Nephritis and IgA Nephropathy”, Denver, CO,
November 2010
Renal Pathology Society Satellite Meeting at the XXVIII International Congress of the
International Academy of Pathology “Infection and Vasculitis”, Sao Paulo, Brazil, October 2010
Annual Meeting of the International Pediatric Nephrology Association, New York, NY,
“Advances in the Pathologic Classification and Diagnosis of Glomerular Diseases”, August 2010
Annual Meeting of the International Pediatric Nephrology Association Renal Pathology Pre-
Course for Pediatric Nephrologists, “Mini Primer on the Pathology of Pediatric Glomerular
Diseases”, “IgM Mesangial Nephropathy and C1q Nephropathy: Distinct or Not?” and
“Vasculitis”, New York, NY, August, 2010
Columbia University Postgraduate Review Course: Renal Biopsy in Medical Diseases of the
Kidney, “ Rapidly Progressive Glomerulonephritis and ANCA” and IgA Nephropathy and H-S
Purpura”, New York, NY, August 2010
Pathogenesis of Vascular Inflammation Induced by Anti-Neutrophil Cytoplasmic
Autoantibodies: and Incentive Compensation Plan of Academic Faculty”, Boston University,
September 2010
“Vasculitis Caused by Antoneutrophil Cytoplasmic Autoantibodies (ANCA): Diagnosis,
Pathogensis and Rationale for Treatment”, Rheumatology Grand Rounds, Duke University,
Durham, NC, September 2010
HARVEY MICHAEL JONES, M.D.
Osler and the Sanitary Movement, American Osler Society, Annual Meeting, Philadelphia, PA,
May 2, 2011
KATHLEEN KAISER-ROGERS, Ph.D.
Structural Chromosome Rearrangements" UNC-Greensboro Genetic Counseling students, 120
minutes 2/17/11
Problem solving conference, UNC-Greensboro Genetic Counseling students, 60 minutes 2/17/11
"Molecular Cytogenetics" UNC-Greensboro Genetic Counseling students, 120 minutes 2/24/11
Problem solving conference, UNC-Greensboro Genetic Counseling students, 60 minutes 2/24/11
DAVID G. KAUFMAN, M.D., Ph.D.
Advances in Enzyme Regulation Meeting, University of Bologna, Bologna, Italy, October 2010
SARA KOENIG, M.D.
Apheresis and Stem Cell Collection, BMT Nursing Core Curriculum
Transfusion Medicine for Clinicians, Pediatric Resident Introductory Series
94
JOE N. KORNEGAY, D.V.M., Ph.D.
“Translational lessons learned from a canine model of Duchenne muscular dystrophy”, Southeast
Veterinary Neurology Meeting, Athens, Georgia, September 25, 2010
“Translational lessons learned from a canine model of Duchenne muscular dystrophy”, Chester
Hartenstein ’45 Memorial Lecture, College of Veterinary Medicine, Cornell University, Ithaca,
NY, October 19, 2010
“A canine model of muscle hypertrophy”, Johns Hopkins University and Krieger Institute,
Baltimore, MD, November 1, 2010
“Translational lessons learned from a canine model of Duchenne muscular dystrophy”, NHLBI,
Bethesda, MD, March 9, 2011
“A canine model of muscle hypertrophy”, Children;s National Medical Center, Washington, DC,
March 10, 2011
“Translational lessons learned from a canine model of Duchenne muscular dystrophy”, One
Health Exchange Series North Carolina Biotechnology Center, May 19, 2011
“Translational lessons learned from a canine model of Duchenne muscular dystrophy”, American
College of Veterinary Internal Medicine, Denver, Colorado, June 15, 2011
NOBUYO MAEDA, Ph.D.
1st International Diabetes and Obesity Forum, Athens, Greece, Oct 21, 2010
Minisymposium on Lipids, Wake Forest University, April 11, 2011
CHRISTOPHER R. McCUDDEN, Ph.D.
Training and Competency Assessment of Laboratory Professionals in the USA. Journee
Internationales de Biologie, CNIT La Defense, Paris, France, November 2010
Discussion Panel Member “Meeting the Needs of Young Laboratorians”. Journee
Internationales de Biologie, Paris, France, November 2010
Clinical Endocrinology for Laboratory Professionals. North Carolina Society for Clinical
Laboratory Science (NCSCLS) Fall Focus 2010, Wake Tech, Raleigh, NC
Serum Protein Electrophoresis: New Therapies, New Challenges. Department of Pathology and
Laboratory, The Ottawa Hospital, Ottawa ON, Canada, October 2010
MELISSA B. MILLER, Ph.D.
Eastern Pennsylvania Branch of the American Society for Microbiology, 40th
Annual
Symposium, “New Molecular Approaches to Identify 21st Century Microbes Directly from
Patient Specimens,” Philadelphia, PA, November 2010
50th
Interscience Conference on Antimicrobial Agents and Chemotherapy, “Best Practices in the
Laboratory Diagnosis of Neisseria gonarrhoeae and Chlamydia trachomatis,” Interactive
Symposium, Boston, MA, September 2010
Southwestern Association of Clinical Microbiology 29th
Annual Meeting, “Challenging Cases in
Clinical Microbiology,” San Antonio, TX, September 2010
International Association of Forensic Nurses 5th
Annual Educational Retreat, “Sexually
Transmitted Infections: Current Trends,” Atlantic Beach, NC, August 2010
95
Memorial Sloan-Kettering Cancer Center, Infectious Disease Seminar, “Clinical Microbiology in
the 21st Century,” New York, NY, July 2010
11th
Annual North Carolina Tuberculosis Symposium, “Molecular Tuberculosis Diagnostics,”
Durham, NC, March 18, 2011.
27th
Annual Clinical Virology Symposium, “Routine implementation of respiratory viral panels,”
Daytona Beach, FL, May 9, 2011.
American Society for Microbiology, 111th
General Meeting, Core Curriculum, “Best practices in
the laboratory diagnosis of viral respiratory tract infections,” New Orleans, LA, May 23, 2011.
Association for Professionals in Infection Control and Epidemiology, 38th
Annual Educational
Conference and International Meeting, “Molecular Microbiology: Uses and Pitfalls,” Baltimore,
MD, June 27, 2011.
Infectious Disease Grand Rounds (co-presented with John Schmitz and Peter Gilligan), “What’s
new in the Clinical Microbiology-Immunology Laboratories,” June 24, 2011.
VOLKER R. NICKELEIT, M.D.
International Academy of Pathology (XXVII International Congress): “Viral Infections and the
Kidney”. Sao Paulo, Brazil, October 2010
Update on Polymavirus Nephropathy: Astelas Pharmaceutical Research Advisory Group
Meeting, December 2010
11th Banff Conference on Allograft Pathology: “Polyomavirusnephropathy - Banff consensus
classification.” June 2011, Paris, France - [invited guest lecturer]
Course on The Practice of Nephropathology (Nephropathologiekurs Volhard-Fahr), lecturer on
‘Transplant Pathology’, Mannheim Germany, April 2011
YARA A. PARK, M.D.
Invited Lecturer, Transfusion Support for Bone Marrow Transplant Patients, University of North
Carolina Hospitals, Department of Medicine, Hematology/Oncology Fellows, 2011
KATHLEEN W. RAO, Ph.D.
Analysis and Clinical Validation: A Laboratory Perspective, Oversight of Clinical Cytogenetic
Array Technologies: An Educational Workshop (ACMG); Bethesda, MD, July 2010
ARLIN B. ROGERS, D.V.M., Ph.D.
Rogers AB. Sex, bugs and toxin roles in liver cancer. Liver Workshop: Hepatic Fibrosis and
Hepatocellular Cancer: Inevitable Consequences of Chronic Liver Injury; ASIP, Experimental
Biology 2011; Washington, DC, Apr 2011 (invited).
Rogers AB. The influence of gut microbes on extraintestinal cancers. Workshop: Health
Consequences from Xenobiotic - Gut Microbiome - Host Interactions; Michigan State University
Superfund Research Program, National Institute of Environmental Health Sciences; Research
Triangle Park, NC, Nov 2010 (invited).
96
Rogers AB. Liver cancer and gut microbiota. Workshop: Human Microbiome, HIV Infection
and Cancer; AIDS Malignancy Program, Office of HIV AIDS Malignancy, National Cancer
Institute, Bethesda, MD, Sep 2010 (invited).
HARSHARAN K. SINGH, M.D.
Nephrology Grand Rounds: Haufen are novel urinary biomarkers of Polyomavirus Nephropathy,
December 2010
OLIVER SMITHIES, Ph.D.
Animal Models of Diabetic Complications Consortium (NIH/AMDCC) Meeting, Baltimore,
Maryland, August 5, 2010
Marshall Forum on Transatlantic Affairs, Keynote Address, Raleigh, NC, “On Being a
Scientist”, September 18, 2010
Dinner of Champions, Keynote Address, An Annual Celebration of the Health and Life Sciences
Benefiting the National Multiple Sclerosis Society, Umstead Hotel & Spa, Raleigh, NC,
September 23, 2010
UNC Science Expo, Keynote Lecture, The Life and Work of a Nobel Prize Winner, September
25, 2010
University of Kentucky, Lexington, Kentucky, College of Pharmacy Research Day, Symposium
on Drug Discovery and Development, “60 Years of Science”, October 15, 2010
BRFAA Institute, Athens, Greece, “On Being a Scientist for 60 Years”, October 22, 2010
BBC Radio for Moments of Genius, Telephone Interview, November 8, 2010
Keynote address at the Eighth Globalization of Pharmaceutics Education Network (GPEN)
hosted by UNC Eshelman School of Pharmacy, “On Being a Scientist for 60 Years”, November
10, 2010
Morehead Event, Jupiter Ball, November 19, 2010
MD/PhD Lecture, December 13, 2010
Interview with D.G. Martin on “Who’s Talking” on WCHL, February 8, 2011
Washington University in St. Louis, MO, Paul E. Lacy Lecture, “On Being a Scientist for 60
Years”, February 21, 2011
Interview for Carolina Week, UNC-CH, on how mice are used in biomedical research, March 4,
2011
Oliver Smithies Nobel Symposium with guest lecturers Drs. Thomas Steitz and Joan Steitz,
UNC-CH, March 7-9, 2011
HHMI Alumni Event, Keynote Lecture, “60 Years as a Bench Scientist”, Museum of Life and
Science, Durham, NC, March 22, 2011
UNC School of Medicine, Leadership in Community Services, “On Being a Scientist for 60
Years”, March 29, 2011
UNC Graduate Student Recognition Event Lecture, UNC-CH, April 6, 2011
Cell and Developmental Biology Departmental Seminar Series, UNC-CH, “On Being a Scientist
for 60 Years”, April 13, 2011
Interview/Video about the UNC Med School Admissions Office, April 20, 2011
97
Investigators’ Appreciation Event, Keynote Lecture, North Carolina Central University, Durham,
NC, April 29, 2011
University of Louisville, College of Pharmacy Research Day, Louisville, Kentucky, “Some
Thoughts on the Kidney”, May 12, 2011
University of Louisville, College of Pharmacy Research Day, Louisville, Kentucky, “On Being a
Scientist for 60 Years”, May 13, 2011
61st International Meeting of Nobel Laureates, Lindau, Germany, “A Toolmaker’s Story”, June
27, 2011
625th
Anniversary of the Ruprecht-Karls University Meeting, Heidelberg, Germany, Special
Lecture
LEIGH B. THORNE, M.D.
Surgical Oncology Conference, East Carolina University, September 2010
DIMITRI G. TREMBATH, M.D., Ph.D.
Trainee Luncheon Speaker, Association Molecular Pathology Meeting, November 2010
CYRUS VAZIRI, Ph.D.
EMBO workshop on DNA Repair, Oxford, United Kingdom, 2012
Grand Rounds Speaker, Moffitt Cancer Center, Tampa, FL, 2010
KAREN E. WECK, M.D.
“The Multidisciplinary Approach to Personized Medicine: Fitting together the pieces of the ‘P-4’
Puzzle”. Conference on Personalized Medicine in the 21st Century, RTI International and the
North Carolina Biotechnology Center, Durham, NC, June 2010
“CYP2D6 Genotyping to Guide use of Taxixifen in Breast Cancer”, Symposium on
Pharmacogenomics in Clinical Proactice, American Association of Clinical Chemistry Annual
Meeting, Anaheim, CA, July 2010
“Case Studies in Molecular Genetics: Cystic Fibrosis Mutation Analysis and BRCA ½
Analysis”, Association if Molecular Pathology Outreach Course, San Jose, CA November 2010
BERNARD E.WEISSMAN, Ph.D.
October 2010- Invited Speaker, 3rd
International Conference of Tumor Targeted Therapy and
Asia-Pacific Forum on Stem Cell, Xi’an, China.
Invited Speaker- NIH SCORE SC1 and SC2 PI Meeting, December 2010
MONTE S. WILLIS, M.D., Ph.D.
“Muscle Ring Finger 1 (MuRF1)’s regulation of Cardiac Hypertrophy, Energy Metabolism, and
Mitrchondrial Function”. University of Washington School of Medicine, Special Seminar, Seattle,
Washington, October 2010
98
“The Use of Animal Models of Duchenne Muscular Dystrophy Muscular Dystrophy Cardiomyopathy to
Test Novel Experimental Therapies.” Seattle Children’s Research Institute, Seattle, WA, October 2010
“The Role of Ubiqyitin Ligase MuRF1 in Regulating Cardiac Mass and PPARα Mediated /energy
Metabolism”. University of Nebraska Medical Center, Omaha Veteran’s Affairs Medical Center,
Omaha, NE, October 2010
“Regulations of Cardiac Hypertrophy and Metabolism by the Ubiquitin Ligase MuRF1.” University of
South Dakota, Graduate Student Association Invitation, Vermillion, SD, October 2010
“Regulation of Cardiac Hypertrophy and Metabolism by MuRF-family of Ubiquitin Ligases”.
University of Calgary, Department of Physiology & Libin Cardiovascular Institute Seminar, Calgary,
Alberta, Canada, August 2010
RUTH E. WINECKER, Ph.D.
Getting Good Data for Suspected Drug Overdose Cases– Helpful Hints. Presented at Death
Investigation Seminar Fitting the Pieces to the Puzzle. Hosted by the Brody School of Medicine at
East Carolina University. Greenville, NC, December 1, 1010.
ALISA S. WOLBERG, Ph.D.
TG Measurement and Fibrin Formation, 1st Maastricht Summer School on Thrombin Generation
and its Application, Maastricht, Netherlands, June, 2010
Plasma and Cellular Influences on Fibrin Structure and Stability, Gordon Research Conference
on Hemostasis, Waterville Valley, NH, July 27, 1010
Cellular and Soluble Contributions to Clot Formation, Structure, and Stability, 13th
Biannual
Midwest Platelet Conference, Chapel Hill, NC, October 15, 2010
Clot Formation, Structure and Stability: Virchow’s Triad Revisited, Hemostasis, Bleeding and
Therapeutic Approaches in 2010: New Therapies and New Paradigms IV, Atlanta, GA,
December 4, 2010
JOHN WOOSLEY, M.D., Ph.D.
Review of Liver Pathology, Pathology and Imaging Course, American College of
Gastrienterology, San Antonio, TX, October 2010
HONG XIAO, M.D.
Invited lecture titled “The Advances in Pathogenesis and Treatment of ANCA
Disease” 9th
National Conference on Geriatric Nephrology, 9-13 June 2011, Wuhan,
China.
XIANWEN YI, M.D., Ph.D.
Type 2 Diabetes, Insulin Resistance and Metabolic Dysfunction (J1)/Obesity (J2), Keystone
Symposium, A Joint Plenary Session, Jan., 2011:
99
MAIMOONA B. ZARIWALA, Ph.D.
Zariwala M. Whole-Exome Sequencing to Identify Genetic Causes of Primary Ciliary
Dyskinesia., International Conference on Inherited Disorders of Muco-Ciliary Clearance (Focus
on PCD), May 20-22, 2011, Münster, Germany.
Zariwala M. Use of Whole-Exome Sequencing to Define Genetic Causes of Primary Ciliary
Dyskinesia. Gordon Research Conference: Cilia, Mucus & Mucociliary Interaction, Feb. 13-18,
2011, Ventura, CA, USA.
Zariwala M: The emerging genetics of primary ciliary dyskinesia. Primary Ciliary Dyskinesia
and Overlapping Syndrome, Sept. 30-Oct. 1, 2010, St. Louis, MO, USA.
DIRECTOR OF CONTINUING EDUCATION COURSES
ARABA N. AFENYI-ANNAN, M.D., Ph.D.
History of Blood Banking, AABB Annual Meeting, Baltimore, MD. October 2010
Immunopathology of Sickle Cell Disease, AABB Annual Meeting, Baltimore, MD. October
2010
WILLIAM B. COLEMAN, Ph.D.
Experimental Biology 2011, April 2011, Washington D.C., Breast Cancer Workshop: Breast
Cancer Stem Cells. Workshop Organizers and Session Chairs: A.G. Rivenbark and W.B.
Coleman.
CHERIE H. DUNPHY, M.D.
Co-Director, Society of Hematopathology USCAP Symposium, March 2011
DAVID EBERHARD, M.D., Ph.D.
“Challenges In Specimen Quality and Handling”, NCI Workshop on Criteria for Use of 'Omics-
Based Predictors in Clinical Trials, Bethesda, MD, June 23-24, 2011
WILLIAM K. FUNKHOUSER, M.D., Ph.D.
UNC CME course – Pulmonary Pathology, 4/30/11
PETER H. GILLIGAN, Ph.D.
A Case-Based Review of GI Infections, SEACM, Charlotte, NC, November 2010
100
Understanding CF Microbiology American Society for Microbiology New Orleans May 2011
Beyond Enterobactericeae: Isolation, Identification, Antimicrobial Susceptibility, and Clinical
Significance of Glucose Non-fermenters and Fastidious Gram Negative Bacilli, NACMID
Springfield MA June 2011
CATHERINE HAMMETT-STABLER, Ph.D.
Contemporary Issues in Clinical TDM/Tox. Chicago, IL. April 2011. Conference chair and
moderator.
J. CHARLES JENNETTE, M.D.
Presiding Officer and Program Committee Chair, Association of Pathology Chairs Annual Meeting,
Pathology Practice and Management: Sharing Successes, Avoiding Failures and Preparing for the
Future”, Seattle, WA, July 2010
Co-Organizer: 15th International Vasculitis and ANCA Workshop, Chapel Hill, NC, May 15-18,
2011
Co-Organizer: Chapel Hill Consensus Conference on Vasculitis Nomenclature, Chapel Hill, NC,
May 14, 2011
CHRISTOPHER McCUDDEN, Ph.D.
Preparation of Manuscripts for Publication: Improving Your Chances for Success. Moderator. AACC
Annual Meeting, Anaheim, CA, July 2010
MELISSA B. MILLER, Ph.D.
Southwestern Association of Clincal Microbiology 29th
Annual Meeting, “Sequence-based
Identifications in Microbiology,” San Antonio, TX, September 2010
VOLKER R. NICKELEIT, M.D.
Short Course #45, United States and Canadian Academy of Pathology (USCAP), continuous
education series: “Diagnosing Tubulointerstitial and Vascular Diseases of the Kidney: a case
based algorithmic approach using virtual microscopy”, San Antonio, TX (March 2011); with HK
Singh
CME: Nephropath Laboratory Staff, August 2010 & November 2010
HARSHARAN K. SINGH, M.D.
Co-Director - United States and Canadian Academy of Pathology (USCAP), Short Course
entitles: “Diagnosing Tubulointerstitial and Vascular Diseases of the Kidney: a case based
algorithmic approach using virtual microscopy”, USCAP Annual Meeting, San Antonio, TX,
February 26 – March 4, 2011
101
KAREN WECK-TAYLOR, M.D.
Course Director, “Current Applications of Molecular Pathology: Real Time Updates and Case Studies,”
Association of Molecular Pathology Outreach Course (1 Day Course), San Jose, CA, November 2010
ALISA S. WOLBERG, Ph.D.
Co-Chair: 6th
Symposium on Hemostasis with Special Focus on Tissue Factor, Factor VIIa, and
Tissue Factor Pathway Inhibitor: Hemostasis and Beyond, May 3-5, 2012
SERVICE ON UNC AND UNCH COMMITTEE
ROBERT C. BAGNELL, Ph.D.
Member, Microscopy Core Labs Sub-Committee
DWIGHT A. BELLINGER, D.V.M., Ph.D.
Member, Institutional Biosafety Committee
Member, Institutional Animal Care and Use Committee
THOMAS W. BOULDIN, M.D.
Member, Graduate Medical Education Committee
Member, North Carolina Cancer Hospital Executive Committee
Member, Full Professors Appointment, Promotion and Tenure Committee
Member, Health Safety Advisory Committee
Chair, Full Professors Appointment, Promotion and Tenure Committee
ARLENE S. BRIDGES, Ph.D.
Chair, UNC Health Sciences Library Advisory Committee
Member, University Library System Advisory Committee
Member, UNC TraCS Core Directors Committee
Member, Committee to Develop HR Tracks for Core Facility Personnel
JOHN F. CHAPMAN, Dr. P.H.
Chair, UNCH Point of Care Committee
Hospital Infection Control Committee, UNC Health Care
Anti-infective Subcommittee of the Pharmacy & Therapeutics Committee and the Department of
Hospital Epidemiology, UNC Health Care
102
FRANK C. CHURCH, Ph.D.
Member, Morehead-Cain Foundation, Central Selection Committee
Member, University Research Council Grants Review Panel
Member, 2nd
year Course Directors Committee (CC2)
Member, Medical School Student Promotions Committee (SPC)
Member, Medical School Admissions Committee
Member, Academy of Distinguished Teaching Scholars, UNC-CH
Member, Executive Committee of the Carolina Cardiovascular Biology Center
Fellow, Academy of Educators, UNC-CH School of Medicine
WILLIAM B. COLEMAN, Ph.D.
Member, Biological and Biomedical Sciences Program Executive Committee, July 2008-Present
Member, Department of Pathology and Laboratory Medicine Research Advisory Committee
MARILA CORDIERO-STONE, Ph.D.
Member, Executive Committee, Biological and Biomedical Science Program
Member, Mentoring Committee
Chair, Promotion/Tenure Review Committee
Executive Committee, Curriculum in Toxicology
GEORGETTE A. DENT, M.D.
Member, Student Promotions Committee
Member, Curriculum Committee
Member, 1st Year Course Directors Committee
Member, 2nd
Year Directors Committee
3rd
& 4th
Course Directors Committee
Assistant Dean for Admission Search Committee
UNC SOM LCME Committees and Working Groups
Chair, UNCH Hospital Infection Committee
Chair, Associate Vice Chancellor of Student Affairs Search Committee
CHERIE H. DUNPHY, M.D.
Member, UNC Fixed Term Promotions Committee
DAVID EBERHARD, M.D., Ph.D.
Member, UNC Committee for the Communication of Genetic Research Results (CCGR)
ROSANN A. FARBER, Ph.D.
Member, Faculty Hearings Committee
103
Member, Faculty Advisory Committee on Postdoctoral Scholars
Member, Department of Genetics Advisory Committee
CRAIG A. FLETCHER, D.V.M., Ph.D.
Member, Institutional Animal Care and Use Committee
PETER H. GILLIGAN, Ph.D.
Chair, IRB, IACUC, SOM Admissions Committee
VIRGINIA GODFREY, D.V.M., Ph.D.
Member, SOM Fixed Term Promotions
Member, Search Committee for DLAM Director
KEVIN G. GREENE, M.D.
Member, MS2 GI Block Planning Committee
MARGARET L. GULLEY, M.D.
Member, UNC Clinical Tranlational Science Award, Section Leader
Member, UNCH RAM Lab Advisory Group to UCRF
Member, Executive Director’s Advisory Group, Department of Pathology and Laboratory
Medicine
SUSAN C. HADLER, M.D., M.S.
Member, 2nd
Year Curriculum Committee (Medical School)
Member, 4th
Year Clinical Capstone Course (Medical School)
Member, Interview MS 2 Students for Ashville Program (Medical)
Member, Assessment Committee (Medical School)
Member, Research in the Medical Curriculum (Medical School)
Member, Dental School Admissions Committee
Member, Dental School 1st Year Teaching Committee
Member, Assessment Revision Committee (Dental School)
CATHERINE A. HAMMETT-STABLER, Ph.D.
Member, School of Medicine 2nd
Year Course Directors
Member, Clinical Documentation Committee
Member, CDC Documentation Sub-Committee
Member, LCME Self-Study Committee, Educational Program Sub-Committee
Chair, IRB, IACUC, SOM, Admissions Committee
104
TRACY HEENAN, D.V.M.
Member, Faculty Council
Member, Division of Laboratory Animal Medicine Director Search
Member, DLAM Advisory Committee
Chair, IACUC Animal Concern Subcommittee
Chair, IACUC/DLAM Leadership Committee
Chair, Vice Chancellor for Research (VCR) Compliance Task Force
Co-Chair, Network of Laboratory Animal Coordinator [NLAC]
Steering Committee
Chair, VCR Development Leadership Mentoring Committee
J. CHARLES JENNETTE, M.D.
Member, UNC Health Care System Executive Council
Member, Dean’s Advisory Committee of the UNC School of Medicine
Member, UNC Physicians & Associates Board
Member, Medical Staff Executive Committee
Member, Hillsborough Hospital Planning Committee
Member, UNC Physicians & Associates Payor Relations Committee
Co-Chair, SOM Strategic Space Committee
WILLIAM K. KAUFMANN, Ph.D.
Member, Research Advisory Committee, DPLM
JOE N. KORNEGAY, D.V.M., Ph.D.
Member, Institutional Animal Care and Use Committee (IACUC)
Member, Department of Pathology and Laboratory Animal Medicine Grand Rounds Committee
Member, Department of Pathology and Laboratory Animal Medicine Research Advisory
Committee
Member, Department of Pathology and Laboratory Animal Medicine Preliminary Exam
Committee
Co-Chair, Division of Laboratory Animal Medicine (DLAM), Director Search Committee
SUSAN T. LORD, Ph.D.
Member, Research Advisory Committee
Member, Advisory Board, Program in Cellular and Molecular Biophysics
Member, Association of Professional Women in the Medical School Executive Committee
Member, Graduate Education Advancement Board
105
CHRISTOPHER P. MACK, Ph.D.
Chair, Dept of Pathology Faculty Assessment
NOBUYO MAEDA, Ph.D.
Member, DLAM Space Committee
Member, Pathology Research Advisory Committee
Member, DLAM Director Search Committee
Chair, DLAM Advisory Committee
SUSAN J. MAYGARDEN, M.D.
Member, GMEC Committee
MELISSA B. MILLER, Ph.D.
Member, Hospital Infection Control Committee, UNC Health Care
Anti-Infective Subcommittee of the Pharmacy & Therapeutics Committee and The Department
of Epidemiology, UNC Health Care
C. RYAN MILLER, M.D., Ph.D.
Member, Lineberger Comprehensive Cancer Center Clinical Genomics
Member, Lineberger Comprehensive Cancer Center Clinincal Informatics
JUDITH N. NIELSEN, D.V.M.
Member, Network of Laboratory Animal Coordinators Steering Committee
Member, Institutional Animal Care and Use Committee
Member, IACUC subcommittee on Pharmaceuticals for Use in Laboratory Animal Research
Ad Hoc, SOM/DLAM Space committee
Member, DLAM Advisory Committee
Member, University-wide Laboratory Animal Strategic Planning/Stakeholder’s Committee
YARA A. PARK , M.D.
Member, Pharmaceutical and Therapeutic Committee
KATHLEEN W. RAO, Ph.D.
Member, Curriculum Committee
Member, Block 9 Course Committee
Co-Chair, 2nd
Year Curriculum Committee
Co-Chair, Academy of Educators
106
HOWARD M. REISNER, Ph.D.
Member, Student Promotions Committee
Member, Medical School Admissions Committee
Member, Second Year Course Directors Committee
Member, IT Governance Communications Technology Coordinating Committee
Member, University Hearings Board
ARLIN B. ROGERS, D.V.M., Ph.D.
Member, Molecular and Cellular Pathology Graduate Program
Member, Preliminary Examination Committee
HARSHARAN K. SINGH, M.D.
Member, Hospital Credentials Committee
SCOTT V. SMITH, M.D.
Member, UNCH Pediatrid Timor Board
Member, UNCH Multidisciplinary Thoracic Oncology Tumor Board
Chair, UNC SOM Student Appeals Committee
JOAN M. TAYLOR, Ph.D.
Member, School of Medicine Appointments for Tenure and Promotions Committee
Member, Health Sciences Affairs APT Committee
Member, School of Medicine Conflict of Interest Committee
Member, Carolina Cardiovascular Biology Center, Executive Committee
Member, Integrative Vascular Biology Training Program Admissions Committee
Member, Medical Student Research Grant Evaluation Committee
Member, Animal Models Core Oversight Committee
LEIGH B. THORNE, M.D.
Member, Core Facilities Oversight Committee
RICHARD R. TIDWELL, Ph.D.
Member, Biomedical IRB Board
Member, UNC-CH Research Advisory Council
Member, UNC-CH Aids Clinical Trials Group
Member, UNC-CH Advisory Board for the Centers for Infectious Disease
Chair, Carolina Center for Clinical Drug Development Advisory Board
107
MICHAEL D. TOPAL, Ph.D.
Chair, UNC Core Facilities Advocacy Committee, 2008-present (monthly)
Chair, UNC Office of Translational Technologies Core Facilities, 2009-present (weekly)
Member, Vice Dean of Research Management Team, 2010-present (weekly)
Member, UNC UCRF Pilot Project Review Committee, 2008-present
Member, UNC RAC (Dean’s Research Advisory Committee), 2008-2010
Chair, UNC Proteomics Review Committee, 2008-2010
Chair, UNC Regional Genomics Facility Committee, 2011 (monthly)
Chair, Committee to Establish Fixed-Term Faculty Positions for Core Directors (monthly)
CYRUS VAZIRI, Ph.D.
Member, Pathogenesis Graduate Admissions Committee
BERNARD E. WEISSMAN, Ph.D.
Member, Curriculum in Toxicology Executive Committee
Member, Pathology and Laboratory Medicine Graduate Education Committee
Chair, Animal Studies Core Facility Advisory Committee
HERBERT C. WHINNA, M.D., Ph.D.
Member, UNCH POC Committee
Member, UNCH Transfusion Committee
Member, UNCH Quality Council
Member, UNCHMSEC
JULIA W. WHITAKER, M.S., D.V.M.
Member, Institutional Animal Care and Use Committee (IACUC)
ALISA S. WOLBERG, Ph.D.
Member, UNC Thrombosis and Hemostasis Program Seminar Series
Member, Biological/Biomedical Sciences Program (BBSP) Pathogenesis Admissions Committee
Member, UNC School of Medicine Strategic Planning Task Force #7
Member, Liaison Committee on Medical Education (LCME) Steering Committee
108
The Department of Pathology and Laboratory Medicine has established an online faculty
handbook. The handbook is updated regularly as new information becomes available. The
idea for this handbook came from the faculty, who wished to have a centralized, easily
accessible source of information on topics of interest for new and established faculty
members. The Faculty Handbook provides our faculty members with detailed and up-to-
date information on such topics as faculty appointments, promotion policies, School of
Medicine policies, purchasing, grant proposals, human resources, equipment available
within the Department, and core research services available within the Department,
School of Medicine, and University. The handbook also provides an introduction and
overview of the faculty orientation process. The Department of Pathology and Laboratory
Medicine’s Faculty Handbook is accessible though the Departmental web site.
DEPARTMENTAL FACULTY HANDBOOK
109
DEPARTMENTAL WEB SITE
The Departmental web site (http://www.pathology.unc.edu) was inaugurated in 1995 as a means
of making potential applicants more aware of our graduate, postdoctoral, and residency training
programs. Today, the web site is a comprehensive, detail-rich resource for those seeking
information about the educational, research, and clinical training programs of the Department.
The web site includes information on the residency training program, the thirteen fellowship
and research symposia.
The web site is maintained by Dr. Thomas Bouldin. In June, 2010, the web site was moved to a
server maintained by the UNC School of Medicine. Web pages for the graduate program are
authored by Dr. William Coleman and Dr. Jonathon Homeister. Web pages for the residency and
fellowship training programs and for the faculty are maintained by Dr. Bouldin. Individual
faculty members now have the ability to construct and edit their own biographical sketches and
laboratory web pages on the Departmental web site.
111
PUBLICATIONS
Department of Pathology and Laboratory Medicine
School of Medicine
University of North Carolina at Chapel Hill
July 1, 2010 – June 30, 2011
ARABA AFENYI-ANNAN, M.D.
Afenyi-Annan A, Willis M. Transfusion Medicine Overview. In: Willis MS, Wians FH, eds.
ASCP Caseset Laboratory Medicine. Ohio, ASCP Press; 2011: 200-206. In Press.
DWIGHT A BELLINGER, D.V.M., Ph.D.
Nichols TC, Raymer RA, Franck HW, Merricks EP, Bellinger DA, DeFriess N, Margaritis P,
Arruda VR, Kay MA, High KA. Prevention of spontaneous bleeding in dogs with haemophilia A
and haemophilia B. Haemophilia. 2010 May;16 Suppl 3:19-23. Review
JESSICA BOOKER, Ph.D.
Kimani JW, Buchman CA, Booker JK, Huang BY, Castillo M, Powell CM, Weck KE.
Sensorineural hearing loss in a pediatric population: association of congenital cytomegalovirus
infection with intracranial abnormalities. Arch Otolaryngol Head Neck Surg. 2010 Oct;136(10):
999-1004.
Booker JK. Monitoring Engraftment of Bone Marrow Transplant by DNA Fingerprinting in
Dunphy CH (ed), Molecular Pathology of Hematolymphoid Diseases, Springer, New York,
2010, Chapter 12, pp. 173-176.
THOMAS W. BOULDIN, M.D.
Kumar D, Bouldin TW, Berger RG. A case of progressive multifocal leukoencephalopathy in a
patient treated with infliximab. Arthritis Rheum. 2010 Nov;62(11):3191-5.
Bouldin, TW. The Peripheral Nervous System. In: Rubin’s Pathology: Clinicopathologic
Foundations of Medicine, 6th ed., R. Rubin and D.S. Strayer (eds.), Lippincott Williams &
Wilkins, Philadelphia, 2012, pp. 1383–1392.
ARLENE BRIDGES, Ph.D.
Zamboni W, Combest A, Edwards R, Bridges A, Zamboni B, Walko C, Zorn K, Sukumvanich P,
Krivak T, Kelly J, "Pharmacokinetic, pharmacogenetic, and phenotypic study of docetaxel in
patients with ovarian cancer", Gynecologic Oncology, [Epub, 2011].
112
McRae M, Rezk N, Bridges A, Corbett A, Tien H, Brouwer K, Kashuba A, "Plasma bile acid
concentrations in patients with human immunodeficiency virus infection receiving protease
inhibitor therapy: possible implications for hepatotoxicity", Pharmacotherapy, 30:17-24, 2010.
JOHN F. CHAPMAN, Dr.P.H.
McCudden CR, Voorhees PM, Hainsworth SA, Whinna HC, Chapman JF, Hammett-Stabler CA,
Willis MS. Cross-reactivity of monoclonal antibody therapies with serum protein electrophoresis
tests (Letter). Clin Chem. 2010;56(12):1900-1902.
FRANK C. CHURCH, Ph.D.
McEachron TA, Church FC and Mackman N. (2011) Regulation of thrombin-induced
plasminogen activator inhibitor-1 in 4T1 murine breast cancer cells. Blood Coagul Fibrinolysis.
22:576-82, PMID: 21799402.
Rein, CM, Desai UR, and Church FC (2011) Serpin-glycosaminoglycan interactions. Meth.
Enzymol. In press.
Cardenas JC, Owens AP, Krishnamurthy J, Sharpless NE, Whinna HC. and Church FC. (2011)
Overexpression of the cell cycle inhibitor p16INK4a
promotes a prothrombotic phenotype
following vascular injury in mice. Arterioscler. Thromb. Vasc. Biol. 31(4):827-33. Epub 2011
Jan 13. PMID: 21233453
Rau JC, Mitchell, JW, Fortenberry YM, and Church FC. (2011) Heparin cofactor II: Discovery,
properties, and role in controlling vascular homeostasis. Sem. Thromb. Hemost. 37(4):339-348.
Fortenberry YM, Cook AB, and Church FC (2011) Protein C inhibitor inhibits factor VIIa when
bound to tissue factor. J. Thromb. Haemost. Apr;9(4):861-3. PMID: 21251198
Machlus KR, Cardenas JC, Church FC and Wolberg AS. (2011) Causal relationship between
hyperfibrinogenemia, thrombosis, and resistance to thrombolysis in mice. Blood. May
5;117(18):4953-63. Epub 2011 Feb 25. PMID: 21355090.
McEachron TA, Pawlinski R, Richards KL, Church FC and Mackman N.(2010) Protease-
activated receptors mediate crosstalk between coagulation and fibrinolysis. Blood. 2010 Dec
2;116(23): 5037-5044. Epub 2010 Aug 24. PMID: 20736455
Fortenberry YM, Brandal S, Bialas RC, and Church FC. (2010) Protein C inhibitor regulates
both cathepsin L activity and cell-mediated tumor cell migration. Biochimica Biophysica Acta.
Jun;1800(6):580-90. Epub 2010 Mar 15.
WILLIAM B. COLEMAN, Ph.D.
Best DH, and Coleman WB. (2010) Liver regeneration by small hepatocyte-like progenitor
113
cells after necrotic injury by carbon tetrachloride in retrorsine-exposed rats. Exp. Mol. Pathology
89:92-98.
Jahn JE, Best DH and Coleman WB. (2010) Exogenous expression of Synaptogmin XIII
suppresses the neoplastic phenotype of a rat liver tumor cell line through molecular pathways
related to mesenchymal to epithelial transition. Exp. Mol. Pathology 89:209-216.
Ryan JL, Jones RJ, Kenney SC, Rivenbark AG, Tang W, Knight ERW, Coleman WB and Gulley
ML. (2010) Epstein-Barr virus-specific methylation of human genes in gastric cancer cells.
Infectious Agents and Cancer 5:27.
Rivenbark AG and Coleman WB. (2010) Neoplasms: Principles of development and diversity.
Archives Pathol. Lab. Med. 134:1084-1085.
Kuemmerle NB, Rysman E, Lombardo PS, Flanagan AJ, Lipe BC, Wells WA, Pettus JR,
Froehlich HM, Memoli VA, Morganelli PM, Swinnen JV, Timmerman L, Chaychi L, Fricano
CJ, Eisenberg BL, Coleman WB, and Kinlaw WB. (2011) Lipoprotein lipase links dietary fat to
solid tumor cell proliferation. Molecular Cancer Therapeutics 10:427-436.
Sandhu R, Rivenbark AG, and Coleman WB. (2011) Enhancement of chemotherapeutic efficacy
in hypermethylator breast cancer cells through targeted and pharmacologic inhibition of
DNMT3b. Breast Cancer Research and Treatment. 2011Feb 27 (Epub ahead of print P<ID:
21359954)
The Molecular Basis of Human Cancer, Second Edition, Coleman WB and Tsongalis GJ. (eds.)
Humana Press – Springer (New York), 2011 (In Press). (57 Chapters, 75 Contributors).
Hematopathology: Genomic Mechanisms of Neoplastic Diseases, Crisan D(ed.), Molecular and
Translational Medicine, Coleman WB and Tsongalis GJ (Series Editors), Humana Press –
Springer (New York), ISBN 978-1-60761-261-2, c2010.
Pharmacogenomic Testing in Current Clinical Practice: Implementation in the Clinical
Laboratory, Wu AHB and Yeo K-TJ (eds.), Molecular and Translational Medicine, Coleman WB
and Tsongalis GJ (Series Editors), Humana Press – Springer (New York), ISBN 978-1-60761-
283-4, c2011.
Exercise Genomics, Pescatello LS and Roth SM(eds.), Molecular and Translational Medicine,
Coleman WB and Tsongalis GJ. (Series Editors), Humana Press – Springer (New York), ISBN
978-1-60761-354-1, c2011.
Targeted Therapies: Mechanisms of Resistance, Gioeli D(ed.), Molecular and Translational
Medicine, Coleman WB and Tsongalis GJ (Series Editors), Humana Press – Springer (New
York), ISBN 978-1-60761-477-7, c2011.
114
Tsongalis GJ and Coleman WB. (2011) Nucleic acid probes used in the analysis of
deoxyribonucleic acid. In: Contemporary Practice in Clinical Chemistry, Second Edition, W.
Clarke (ed.), AACC Press, Washington, DC, pp. 153-161.
Tsongalis GJ and Coleman WB (2011) The polymerase chain reaction. In: Contemporary
Practice in Clinical Chemistry, Second Edition, W. Clarke (ed.), AACC Press, Washington, DC,
pp. 163-169.
Best DH and Coleman WB (2011) Adult liver stem cells. In: Molecular Pathology of Liver
Diseases, S.P.S. Monga (ed.), Molecular Pathology Library (P.T. Cagle, series editor), Volume
5, Springer Publishing, New York, pp. 243-260.
Coleman WB and Tsongalis GJ (2011) Cancer epidemiology: Incidence and etiology of human
neoplasms. In: The Molecular Basis of Human Cancer, Second Edition, Coleman WB and
Tsongalis GJ (eds.), Humana Press, Totowa, NJ, (In Press).
Coleman WB and Tsongalis GJ (2011) The role of genomic instability in the development of
human cancer. In: The Molecular Basis of Human Cancer, Second Edition, Coleman WB and
Tsongalis GJ (eds.), Humana Press, Totowa, NJ, (In Press).
Coleman WB and Grisham JW (2011) The molecular basis of liver cancer. In: The Molecular
Basis of Human Cancer, Second Edition, Coleman WB and Tsongalis GJ (eds.), Humana Press,
Totowa, NJ, (In Press).
Best DH and Coleman WB (2011) Cytokine-dependent mechanisms of activation of small
hepatocyte-like progenitor cells. In: Vitamins and Hormones, Volume 87 – Stem Cell
Regulators, G. Litwack (ed.), Academic Press – Elsevier, San Diego, (In Press).
MARILA CORDEIRO-STONE, Ph.D.
Smith-Roe SL, Patel SS, Simpson DA, Zhou Y, Rao S, Ibrahim JG, Kaiser-Rogers KA,
Cordeiro-Stone M, Kaufmann WK. Timeless functions independently of the Tim-Tipin complex
to promote sister chromatid cohesion in normal human fibroblasts. Cell Cycle, 2011 May
15;10(10):1618-24. Epub 2011 May 15.
http://www.landesbioscience.com/journals/cc/Smith-RoeCC10-10.pdf
GEORGETTE A. DENT, M.D.
Blatt J, Greenwood R, Weig S, Rao K, Fedoriw GD, Dent G. Isolated central nervous system
relapse in an adolescent with acute myelomonocytic leukemia, Charcot Marie Tooth syndrome,
and paraneoplastic autoantibody. J Pediatr Hematol Oncol. 2010 Oct;32(7):571-3.
Steiner BD, Carlough M, Dent G, Peña R, Morgan DR. International crises and global health
electives: lesions for faculty and institutions. Acad Med. 2010 Oct;85(10):1560-3.
115
Newton WP, Stone K, Dent GA, Shaheen NJ, Byerley J, Gilliland KO, Rao K, Farrell T, Cross
A. The University of North Carolina at Chapel Hill School of Medicine Acad Med. 2010
Sep;85(9 Suppl):S424-9.
Dent GA, Eby CS: Laboratory hematology In: American Society of Hematology Self-
Assessment Program, 4th Edition, Cadmus Communications, PA, 2010, Chapter 10, pp. 263-
289.
MEGAN J. DiFURIO, M.D.
Gonzalez MN, DiFurio MJ, Sundborg MJ, Leath CA. Undifferentiated endometrial sarcoma
presenting as pathologic humerus fracture. Mil Med. 2010 Sep;175(9):691-692.
CHERIE H. DUNPHY, M.D.
Dunphy CH. Comparative Analysis of Detecting Monocytic Cells and Their Aberrancy by Flow
Cytometry and Immunohistochemistry. Applied Immunohistochemistry and Molecular
Morphology. 2011;19:336-40
Jima DD, Zhang J, Jacobs C, Richards KL, Dunphy CH, Choi WW, Au WY, Srivastava G,
Czader MB, Rizzieri DA, Lagoo AS, Lugar PL, Mann KP, Flowers CR, Bernal-Mizrachi L,
Naresh KN, Evens AM, Gordon LI, Luftig M, Friedman DR, Weinberg JB, Thompson MA, Gill
JI, Liu Q, How T, Grubor V, Gao Y, Patel A, Wu H, Zhu J, Blobe GC, Lipsky PE, Chadburn A,
Dave SS. Deep Sequencing of the Small RNA Transcriptome of Normal and Malignant Human
B Cells Identifies Hundreds of Novel MicroRNAs. Blood. 2010;116: e118-27 Aug 23(Epub
ahead of print).
Dunphy CH (ed). Molecular Pathology of Hematolymphoid Diseases. Series: Molecular
Pathology Library, Vol. 4, 1st Edition., May, 2010, XIX, 1837 pages, 58 illus., 29 in color,
Hardcover, ISBN: 978-1-4419-5697-2.
Dunphy CH (ed). Molecular Pathology of Hematolymphoid Diseases. Techniques to Detect
Chromosomal Translocations (Routine Cytogenetics, FISH, SKY, PCR, IHC), 2010, Chapter 24.
Dunphy CH (ed). Molecular Pathology of Hematolymphoid Diseases. Gene Expression
Profiling, 2010, Chapter 28.
Dunphy CH (ed). Molecular Pathology of Hematolymphoid Diseases, Diffuse Large B Cell
Lymphoma, 2010, Chapter 37.
Dunphy CH. Myeloid Proliferations Related to Down Syndrome. eMedicine from WebMD.
Updated May 14, 2010(http://emedicine.medscape.com/article/1644124-overview).
Dunphy CH. Other Myeloid Related Precursor Neoplasms. eMedicine from WebMD. Updated
May 14, 2010 (http://emedicine.medscape.com/article/1644158-overview).
116
Ju E, Gold S, Dunphy C, Morrell D. Anaplastic Large Cell Lymphoma: An Unusual Presentation
in a 7 Year Old Girl. Pediatric Dermatology. Accepted for Publication, January 12, 2011.
Montes-Morenol S, Martinez N, Sanchez-Espiridión B, Saez A, Montalbán C, Rodriguez, H,
Pisano, D Gomez G, Díaz Uriarte R, Sanchez L, Diaz Perez JA, Conde E, Gonzalez-Barca E,
Lopez A, Ruiz-Marcellan MC, Canales M, Mazorra P, Cruz M, Mollejo M, Martinez MA,
Grande C, Dunphy CH, Hsi ED, Rocque GB,Waknitz M, Xu Z, Chang J, Young KH, Piris MA.
MicroRNA expression in Diffuse Large B Cell Lymphoma treated with chemoimmunotherapy.
Blood. In Press (Accepted for Publication, May 13, 2011).
GEORGE FEDORIW, M.D.
McCudden C, Laramore A, Fedoriw Y. Hyperviscosity Syndrome: Analytical Challenges and
Diagnosis. ASCP: Check Sample. 2010 Nov.
Fender J, Willis MS, Fedoriw Y. Urine Crystals in a 1-Year-Old Male. Lab Medicine.
2010;41:388-392.
Meteesatien P, Plevy SE, Fender JD, Fedoriw Y. Circulating Blasts in a Chron’s Patient Treated
with Natalizumab. Lab Medicine. 2010;41:453-456.
Pendse A, Fedoriw Y, Willis MS. Unexpected Cause of Anemia in a 45-Year-Old Patient with
Acute Lymphoblastic Leukemia. Lab Medicine. 2010;41:453-456.
Blatt J, Spencer W, Fedoriw GD, Rao K, Dent G, Greenwood R. Central Nervous System
Relapse in an Adolescent with Acute Myelomonocytic Leukemia, Charcot Marie Tooth
Syndrome, and Paraneoplastic Autoantibody. Journal of Pediatric Hematology/Oncology. 2010
Aug 18.
Fedoriw YD: Hematology/Coagulation Overview in: Willis MS, Wians FH (eds), ASCP Caseset
Laboratory Medicine, ASCP Press, Ohio, 2011, pp. 463-466.
Liu Y, Johnson SM, Fedoriw Y, Rogers A, Yuan H, Krishnamurthy J, Sharpless NE. Expression
of p16INK4a
prevents cancer and promotes aging in lymphocytes. Blood. 2011 Mar 24;
117(12):3257-3267.
Fedoriw Y, Dunphy CH. Anemia of chronic renal insufficiency. In: Krause J, Filicko-O’Hara J,
and Gulati G, eds. Case Studies in Hematology and Coagulation. ASCP Press, Chicago, IL. In
press; 6 pages.
Fedoriw Y, Dunphy CH. Anemia of chronic liver disease. In: Krause J, Filicko-O’Hara J, and
Gulati G, eds. Case Studies in Hematology and Coagulation. ASCP Press, Chicago, IL. In press;
5 pages.
117
Dunphy CH, Fedoriw Y. Anemia associated with Parvovirus infection. Krause J, Filicko-O’Hara
J, and Gulati G, eds. Case Studies in Hematology and Coagulation. ASCP Press, Chicago, IL. In
press; 5 pages.
Fedoriw Y, Gulley ML. HIV-associated Hodgkin Lymphoma: In: Schrijver I ed. Diagnostic
Molecular Pathology in Practice. 1st edition 2011 Springer-Vonderlag, New York, NY In press;
6 pages.
CRAIG A. FLETCHER, D.V.M., Ph.D.
Gabrielson, KL, Fletcher CA, Czoty P, Nader M, Gluckman, T. (2011) In vivo imaging
applications for the nervous system in animal models. In: Fundamentals of Toxicologic
Neuropathology, Bolon B and Butt M., editors 1st Edition, Wiley-Blackwell, Hoboken, NJ; (In
press)
WILLIAM FUNKHOUSER, Jr., M.D., Ph.D.
Sheahan T, Whitmore A, Long K, Ferris M, Rockx B, Funkhouser W, Donaldon E, Gralinski L,
Collier M, Heise M, Davis N, Johnston R, Baric RS. Successful vaccination strategies that
protect aged mice from lethal influenza and lethal heterologous SARS-CoV challenge. J Virol.
PMID: 20980507.
Wilkerson MD, Yin X Hoadley KA, Liu Y, Hayward MC, Cabanski CR, Muldrew KL, Miller
CR, Randell SH, Socinski MA, Parsons AM, Funkhouser WK, Lee CB, Rober PJ, Thorne L,
Bernard PS, Perou CM, Hayes DN. Lung Squamous Cell Carcinoma mRNA Expression
Subtypes Are Reproducible, Clinically Important, and Correspond to Different Normal Cell
Types. 2010 Jul; Clin Cancer Res 16:4864-75
O’Neil BH, Funkhouser WK, Calvo BF, Meyers MO, Kim HJ, Goldberg RM, Bernard SA,
Caskey L, Deal AM, Wright F, Baldwin AS, Tepper JE. Nuclear Factor Kappa-light Chanin-
enhancer of Activated B Cells is Activated by Radiotherapy, and Is Prognostic for Overall
Survival in Patients with Rectal Cancer Treated with Preoperative Fluorouracil-based
Chemoradiotherapy. Int J Radiat Oncol Biol Phys. 2011 80:705-711 Jul. (Epub ahead of print)
PMID: 20630669.
Roche JP, Younes MN, Funkhouser WK, Weissler MC. Branchiogenic Carcinoma of a First
Branchial Cleft Cyst. Otolaryngol Head Neck Surg. 2010 Jul;143(1):167-8. PMID: 20620640.
McKay JD et al., A genome-wide association study of upper aerodigestive tract cancers
conducted with the INHANCE consortium, PLoS Genetics 2011;7:1. PMID: 1000849
ASIP Milestones article, “JFAP Miller and the Thymus”, 2011 Jun.
118
PETER H. GILLIGAN, Ph.D
York MK, Gilligan PH: Blood cultures: General Detection and Interpretation in L Garcia (ed),
Clinical Microbiology Procedure Handbook, 3rd
Edition, ASM Press, Washington, D.C., 2010,
Chapter 3-4.1.
Gilligan PH, York MK, Church DL. Brucella Cultures in L Garcia (ed), Clinical Microbiology
Procedure Handbook, 3rd
Edition, ASM Press, Washington, D.C., 2010, Chapter 3.4.2.
York MK, Gilligan PH, Welch DF. Bartonella Cultures in L Garcia (ed), Clinical Microbiology
Procedure Handbook, 3rd
Edition, ASM Press, Washington, D.C., 2010, Chapter 3.4.3.
Gilligan, PH. Laboratory Procedure for Isolation and Identification of Micro-Organisms from
Patients with Cystic Fibrosis in L Garcia (ed), Clinical Microbiology Procedure Handbook, 3rd
Edition, ASM Press, Washington, D.C., 2010, Chapter 3.11.3.1.
York MK, Gilligan PH. Nasal Sinus Cultures in L Garcia (ed), Clinical Microbiology Procedure
Handbook, 3rd
Edition, ASM Press, Washington, D.C., 2010, Chapter 3.11.9.
York MK, Gilligan PH, Church DL. Lower Respiratory Tract Cultures in L Garcia (ed), Clinical
Microbiology Procedure Handbook, 3rd
Edition, ASM Press, Washington, D.C., 2010, Chapter
3.11.2.
York MK, Gilligan PH. Guidelines for the Performance of Repiratory Tract Cultures in L Garcia
(ed), Clinical Microbiology Procedure Handbook, 3rd
Edition, ASM Press, Washington, D.C.,
2010, Chapter 3-11.1.
Gilligan PH, York MK. Meliodosis (burkholderia pseudomallei) and Glanders (burkholderia
mallei) in L Garcia (ed), Clinical Microbiology Procedure Handbook, 3rd
Edition, ASM Press,
Washington, D.C., 2010, Chapter 16-9.
Gray LD, Gilligan PH, Fowler WC. Cumitech 13b: Laboratory Diagnosis of Ocular Infections
ASM Press, Washington D.C., 2010.
Esther Jr, CR, Hoberman S,Fine J, Allen S, Culbreath K, Rodino K, Kerr A, and Gilligan P.
Detection of rapidly growing mycobacteria in routine cystic fibrosis cultures. J. Clin Microbiol.
2011 Apr;49(4):1421-5.
Banet N, Gordon C, Willis M, Gilligan P, and Thorne L. 2011. Unexpected Death in a Heart
Transplant Recipient. Lab Medicine 2011;42. In press.
Noillat Blanco, Prod’hom G, Connell J, De Gascun CF, Gilligan P, and Greub G. Diagnostic
Microbiology for Infections in Cardiothoracic Transplatns and Mechanical Circulatory Support
Recipients. 2011; ISHLT Mono Series 5: 327-346.ISHLT Monograph Series 5: 327–346
119
OLEG V. GORKUN, Ph.D.
Falvo, MR, Gorkun, OV and Lord, ST. The molecular origins of the mechanical properties of
fibrin. Biophysical Chemistry. 2010; 152:15.
Averett LE, Akhremitchev BB, Schoenfisch MH, Gorkun OV. Calcium dependence of fibrin
nanomechanics: the γ1 calcium mediates the unfolding of fibrinogen induced by force applied to
the "A-a" bond. Langmuir. 2010; 26:14716.
PAMELA GROBEN, M.D.
Mitchell CB, Isenstein A, Burkhart CN, Groben P, Morrell DS. Infection with Mycobacterium
immunogenum following a tattoo. J Am Acad Dermatol. 2011 May;64(5):e70-1.
Wertman R, Miller M, Groben P, Morrell DS, Culton DA. Mycobacterium
bolletii/Mycobacterium massiliense furunculosis associated with pedicure footbaths: a report of 3
cases. Arch Dermatol. 2011 Apr;147(4):454-8.
Conway K, Edminston SN, Khondker ZS, Groben PA, Zhou X, Chu H, Kuan PF, Hao H, Carson
C, Berwick M, Ollila DW, Thomas NE. DNA-methylation profiling distinguishes malignant
melanoma from benign nevi. Pigment Cell Melanoma Res 2011 Apr;24(2):352-60.
Mollet T, Henderson FW, Groben PA, Burkhart CN, Morrell DS. Epidermolysis bullosa nevus-
like lesions in a pediatric patient with pyoderma gangrenosum. Pediatr Dermatol. 2011 Jan-
Feb;28(1):32-4.
Weaver CH, Merritt BG, Groben PA, Morrell DS. A partially regressed, atrophic plaque on a 17-
year old girl; an unusual presentation of myofibromatosis. Pediatr Dermatol. 2010 Sep-
Oct;27(5):481-4.
Nicholas MW, Isetnstein A, Dasher D, Granko RP, Lugo-Somolinos A, Groben P, Morrell D..
Varenicline-induced drug eruption with resulting palmar/plantar hyperhidrosis and dysthesia. J
Am Acad Dermatol. 2010 Jul; 63(1):e5-7.
Peppercorn, AF, Miller MB, Fitzgerald D, Weber DJ, Groben PA, Carins BA. High-level human
herpesvirus-6 viremia associated with onset of Stevens-Johnson syndrome. J Burn Care Res.
2010 Mar-Apr;31(2):365-8.
Thomas NE, Kanetsky PA, Edmiston SN, Alexander A, Begg CB, Groben PA, Hao H, Busam K,
Ollila DW, Berwick M, Conway K. Relationship between Germline MC1R Variants and BRAF-
Mutant Melanoma in a North Carolina Population-Based Study. J Invest Dermatol. 2010
May;130(5):1463-5.
120
MARGARET L. GULLEY, M.D.
Sharma S, Gulley ML. BRAF Mutation Testing in Colorectal Cancer, Arch Pathol Lab Med
2010;134:1225-8.
Ma SD, Hegde S, Young KH, Sullivan R, Rajesh D, Zhou Y, Jankowska-Gan E, Burlingham
WJ, Sun X, Gulley ML, Tang W, Gumperz JE, Kenney SC. A New Model of EBV Infection
Reveals an Important Role for Early Lytic Viral Protein Expression in the Development of
Lymphomas. J Virol. 2010 Oct 27. [Epub ahead of print] PMID: 20980506
Gulley ML. Molecular Pathology of Hematolymphoid Diseases: Post-Transplant
Lymphoproliferative Disorder in C. Dunphy (ed), Springer, New York, 2010, Chapter 28,
pp.359-365.
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Reisner HM (Editor) Pathology, A Modern Case Study. 1st edition. McGraw-Hill Company Inc.
In preparation for target publication January 2012. This book incorporates chapters written by
experts in Pathology from the UNC system. I personally designed the format and sold the
concept to the publisher. This book will be accompanied by a selection of virtual slides available
on a server hosted by Aperio.
Rubin E, and Reisner HM. (Editors) Essentials of Pathology, 6th Edition. Lippincott, Williams
& Wilkins, (In preparation for target publication Fall 2011-Spring 2012) This will be the second
time I have prepared a new version of the Essentials working with Dr Rubin.
ARLIN ROGERS, D.V.M., Ph.D.
Ohtani M, Ge Z, García A, Rogers AB, Muthupalani S, Taylor NS, Xu S, Watanabe K, Feng Y,
Marini RP, Whary MT, Wang TC, Fox JG. 17-estradiol suppresses Helicobacter pylori-induced
gastric pathology in male hypergastrinemic INS-GAS mice. Carcinogenesis. 2011;32:1244-1250
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Liu Y, Johnson SM, Fedoriw Y, Rogers AB, Yuan H, Krishnamurthy J, Sharpless NE.
Expression of p16INK4a prevents cancer and promotes aging in lymphocytes. Blood. 2011 Mar
24;117(12):3257-67. PubMed PMID: 21245485.
Moss SF, Moise L, Lee DS, Kim W, Zhang S, Lee J, Rogers AB, Martin W, De Groot AS.
HelicoVax: Epitope-based therapeutic Helicobacter pylori vaccination in a mouse model.
Vaccine. 2011 Mar 3;29(11):2085-91. PubMed PMID: 21236233.
Rogers AB. Distance burning: how gut microbes promote extraintestinal cancers. Gut Microbes.
2011 January/February;2(1):52-57.
Okumura T, Ericksen RE, Takaishi S, Wang SS, Dubeykovskiy Z, Shibata W, Betz KS,
Muthupalani S, Rogers AB, Fox JG, Rustgi AK, Wang TC. K-ras mutation targeted to gastric
tissue progenitor cells results in chronic inflammation, an altered microenvironment, and
progression to intraepithelial neoplasia. Cancer Res. 2010 Nov 1;70(21):8435-45. PubMed
PMID: 20959488.
Patterson MM, Rogers AB, Fox JG. Experimental Helicobacter marmotae infection in A/J mice
causes enterohepatic disease. J Med Microbiol. 2010 Oct;59(Pt 10):1235-41. PubMed PMID:
20616187.
DENNIS SIMPSON, Ph.D.
Smith-Roe SL, Patel SS, Simpson DA, Zhou YC, Rao S, Ibrahim JG, Kaiser-Rogers KA,
Cordeiro-Stone M, Kaufmann WK. (2011) Cell Cycle 10, 1618-1624
Bower JJ, Zhou Y, Zhou T, Simpson DA, Arlander SJ, Paules RS, Cordeiro-Stone M, Kaufmann
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Bower JJ, Karaca GF, Zhou Y, Simpson DA, Cordeiro-Stone M, Kaufmann WK. (2010)
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HARSHARAN SINGH, M.D.
Singh HK, Kozlowski T, True K, Derebail V, Gasim A, Nickeleit V. Urinary Polyomavirus
Haufen Shedding Accurately Reflects Intrarenal Burden of Polyomavirus Nephropathy (PVN):
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SCOTT V. SMITH, M.D.
Rothlein LR, Shaheen AW, Vavalle JP, Smith SV, Renner JB, Shaheen NJ, Tarrant TK:
Sclerosing mesenteritis successfully treated with a TNF antagonist. BMJ Case Reports.
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OLIVER SMITHIES, Dr.Phil.
Wang CH, Li F, Hiller S, Kim HS, Maeda N, Smithies O, Takahashi N. A modest decrease in
endothelial NOS in mice comparable to that associated with human NOS3 variants exacerbates
diabetic nephropathy. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):2070-5. Epub 2011 Jan 18.
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Hatada S, Walton W, Hatada T, Wofford A, Fox R, Liu N, Lill MC, Fair JH, Kirby SL, Smithies
O. Therapeutic benefits in thalassemic mice transplanted with long-term-cultured bone marrow
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Konno T, Chen D, Wang L, Wakimoto H, Teekakirikul P, Nayor M, Kawana M, Eminaga S,
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Heterogeneous myocyte enhancer factor-2 (Mef2) activation in myocytes predicts focal scarring
in hypertrophic cardiomyopathy. Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):18097-102.
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Messadi E, Vincent MP, Griol-Charhbili V, Mandet C, Colucci J, Krege JH, Bruneval P, Bouby
N, Smithies O, Alhenc-Gelas F, Richer C. Genetically determined angiotensin converting
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2010 Jul 28. PMID: 20667972
Smithies O. Science brick by brick, Nature, 2010 Oct 14; 467(7317)S6, PMID: 20944621
Pandya K, Pulli B, Bultman S, Smithies O. Reversible epigenetic modifications of the two
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133
JOAN TAYLOR, Ph.D.
Staus DP, Taylor JM, and Mack CP. Enhancement of mDia2 activity by Rho-kinase-dependent
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Cheng Z, Sundberg-Smith LJ, Mangiante LE, Sayers RL, Hakim ZS, Musunuri S, Maguire CT,
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muscle cell recruitment to the developing vasculature. Arterioscler Thromb Vasc Biol 2011; In
Press
Doherty JT, Lenhart KC, Cameron MV, Mack CP, Conlon FL, Taylor JM. Skeletal Muscle
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Zajac B, Hakim ZS, Cameron MV, Smithies O, and Taylor JM. Quantification of Myocyte
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Medlin MD, Taylor JM., and Mack CP. Quantifying Sphingosine 1-Phosphate-Dependent
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LEIGH THORNE, M.D.
Watson RG, Muhale G, Thorne LB, Yu J, O’Neil BH, Hoskins JM, Meyers MO, Deal AM,
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Subtypes are Reproducible, Clinically Important, and Correspond to Different, Normal Cell
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RICHARD R. TIDWELL, Ph.D.
Yan GZ, Brouwer KL, Pollack GM, Wang MZ, Tidwell RR, Hall JE, Paine MF. Mechanisms
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Wasan EK, Gershkovich P, Zhao J, Zhu X, Werbovetz K, Tidwell RR, Clement JG, Thornton SJ,
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efficacy against visceral Leishmaniasis in a murine model. PLoS Negl Trop Dis. 2011.
4(12):e913.
Reid CS, Patrick DA, He S, Fotie J, Premalatha K, Tidwell RR, Wang MZ, Liu Q, Gershkovich
P, Wasan KM, Wenzler T, Brun R, Werbovetz KA. Synthesis and antitrypanosomal evaluation
of derivatives of N-benzyl-1,2-dihydroquinolin-6-ols: Effect of core substitutions and salt
formation. Bioorg Med Chem. 2011. Jan 1; 19(1):513-523.
Paine MF, Wang MZ, Generaux CN, Boykin DW, Wilson WD, De Koning HP, Olson CA,
Pohlig G, Burri C, Brun R, Murilla GA, Thuita JK, Barrett MP, Tidwell RR. Diamidines for
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Wang MZ, Zhu X, Srivastava A, Liu Q, Sweat JM, Pandharkar T, Stephens CE, Riccio E,
Parman T, Munde M, Mandal S, Madhubala R, Tidwell RR, Wilson WD, Boykin DW, Hall JE,
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Athri P, Wenzler T, Tidwell R, Bakunova SM, Wilson WD. Pharmacophore model for
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2010;
Goldsmith, RB, Gray DR, Yan Z, Gernerauz CN, Tidwell RR, Reisner HM. Application of
monoclonal antibodies to measure metabolism of an anti-trypanosomal compound in vitro and in
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MICHAEL D. TOPAL, Ph.D.
Cancer Genome Atlas Research Network. Integrated genomic analyses of ovarian carcinoma.
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DIMITRI TREMBATH, M.D., Ph.D.
Trembath, DG. Molecular Testing for Brain Tumors. Advance/Laboratory. 2010 Sept; 19(9):12-
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Terry NG, Zhu Y, Rinehart MT, Brown WJ, Gebhart SC, Bright S, Carretta E, Ziefle CG,
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CYRUS VAZIRI, Ph.D.
Palle K and Vaziri C. (2011) Rad18 E3 Ubiquitin Ligase Activity Mediates Fanconi Anemia
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Song IY, Palle K, Gurkar A, Tateishi S, Kupfer GM, and Vaziri C. (2010) Rad18-mediated
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Wong PG, Glozak MA, Cao TV, Vaziri C, Seto E, and Alexandrow M. (2010) Chromatin
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KAREN WECK, M.D.
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Cytomegalovirus Infection with Intracranial Abnormalities. Arch Otolaryngol Head Neck Surg.
2010;136(10):999-1004.
Orsi FA, Annizzio JM, de Paula EV, Ozelo MC, Langley MR, Weck KE. VKORC1 V66M
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Pratt VM, Zehnbauer B, Wilson JA, et al. Characterization of 107 Genomic DNA Reference
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BERNARD WEISSMAN, Ph.D.
Bartlett C, Stammler T, Rosson G S and Weissman BE. BRG1 mutations found in human cancer
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HERBERT C. WHINNA, M.D., Ph.D.
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Miller AE, Montague D, Rodgers JE, Sanghvi S, Whinna HC, Krumnacher H. Substitution of a
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MONTE S. WILLIS, M.D., Ph.D.
Wysong A, Asher SA, Yin X, Gore MR, Weinstein L, Guttridge D, Baldwin A, Couch M, Willis MS.
Selective Inhibition of NF-kappa-B with NBD Peptide Reduces Tumor-Induced Wasting in a Murine
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Li HH, Du J, Fan YN, Zhang ML, Li L, Lockyer P, Kang EY, Patterson C, Willis MS. The Ubiquitin
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Proteasome-Dependent Degradation of Phospho-c-Jun. Am J Pathol. 2011Mar;178(3):1043-58.
Jensen BC, Willis MS. Edge of the World: Practical Considerations and a Clinical Perspective of Next-
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McCudden CR, Voorhees, Hainsworth SA, Whinna HC, Chapman JF, Hammett-Stabler CA, Willis MS.
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Wilson BA, Willis MS. Luminaries in Laboratory Medicine: Percy Lavon Julian Pioneer of Medicinal
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Pendse A, Fedoriw Y, Willis MS. Unexpected Cause of Anemia in a 45-Year-Old Patient with Acute
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Adamo CM, Dai DF, Percival JM, Minami E, Willis MS, Patrucco E, Froehner SC, Beavo JA. Sildenafil
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Banet N, Gordon C, Willis MS, Gilligan P, Thorne L. Unexplained Death in a Heart Transplant
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Banet N, Lininger RA, Willis MS, McCudden CR. Breast Mass in a 38-Year-Old Woman. Lab Med
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Ren R, Willis MS, Fedoriw Y. Episodic Fever and Neutropenia in a 22-Year-Old Male. Lab Med 2010;
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RUTH WINECKER, Ph.D.
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Meyers MO, Yeh JJ, Deal AM, Byerly FL, Woosley JT, Frank J, Long PK, Amos KD, Ollila
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Kozlowski T, Rubinas T, Nickeleit V, Woosley J, Schmitz J,Collins D, Hayashi P, Passannante
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MAIMOONA ZARIWALA, Ph.D.
Berg JS, Evans JP, Leigh MW, Omran H, Bizon C, Mane K, Knowles MR, Weck KE , Zariwala
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