Quick-DNA™
DNA from any Sample
Tel: +49 (0)761 60068710 www.zymoresearch.de
Quick-DNA™ Overview
Learn more at www.zymoresearch.de/dna/quick-dna
Zymo Research offers a range of genomic DNA isolation kits that are suitable for extracting high molecular weight DNA from a wide variety of sample types including tissue, fresh and paraffi n-embedded tissue sections, cultured cells, saliva, buccal cells, whole blood, plasma, serum, urine, bacteria, fungi, yeast, algae, viruses, and mitochondria. Our genomic DNA isolation kits yield high-quality DNA that is ideal for use in any sensitive downstream applications such as PCR, DNA sequencing, endonuclease digestion, and methylation detection.
Biological Fluids,Cells & Tissues
Quick-DNA™ Plus Kits
Liquid Biopsy Serum, Plasma,
Urine, etc.
Quick-cfDNA™ Serum & Plasma Kit
Quick-DNA™
Urine Kit
FFPE Samples Quick-DNA™ FFPE Kit
Cultures
Environmental(Fecal, soil, water fi ltrate and other)
Plants
Quick-DNA™ Fungal/Bacterial DNA Kits
MicrobialQuick-DNA™ Fecal/Soil
Microbe DNA Kits
For DNA isolation in microbiomic studies, see our ZymoBIOMICS™
product line.
Quick-DNA™ Plant/Seed Kits
www.zymoresearch.deTel: +49 (0)761 60068710
Quick-DNA™ Plus KitsHighlights
• Extract high-quality DNA easily and reliably from any biological fl uids, cultured/monolayer cells, or solid tissues
• Zymo-Spin™ technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
vs.
Supplier Q
2-3 μl Contamination
Zymo Research0 μl
Carryover
Zymo-Spin™ technology ensures no carryover of buffer, salts, or other PCR inhibitors. DNA is ready for all sensitive downstream applications such as qPCR, Next-Generation sequencing arrays, and methylation analysis.
DNA Yields Increase Linearly with Increasing Volumes of Human Whole Blood Using the Quick-DNA™ Miniprep Plus Kit. Six replicates of 25, 50, 100, and 200 µl of human whole blood were processed.
Human Whole Blood (µl)
R2=0.9936
8.00
7.00
6.00
5.00
4.00
3.00
2.00
1.00
0.00
0 25050 200100 150
Tota
l Yie
ld (µ
g)
High-quality DNA obtained from a wide range of biological samples using the Quick-DNA™ Miniprep Plus Kit. DNA purifi ed using the Quick-DNA™ Miniprep Plus Kit is ultra-pure, highly concentrated, and ready for all downstream applications. Input DNA was standardized to 300 ng and analyzed in a 1% (w/v) TAE/agarose/EtBr gel. The size marker “M” is a 1 kb ladder (Zymo Research).
HumanBlood
PorcineBlood
HeLaCells
BuccalSwab
HumanSaliva
MouseTail
MouseKidney
MouseBrain
BovineMuscle
BovineMilk M
Product Cat. No. SizeQuick-DNA™ Microprep Plus Kit D4074 50 preps.
Quick-DNA™ Miniprep Plus Kit
D4068T 10 preps.
D4068 50 preps.
D4069 200 preps.
Quick-DNA™ Miniprep Kit(no Proteinase K)
D3024 50 preps.
D3025 200 preps.
Learn more and view additional Quick-DNA™ Kit formats at www.zymoresearch.de/dna/quick-dna
Purity Redefi ned Reliable & Consistent
High-quality DNA from any sample
www.zymoresearch.deTel: +49 (0)761 60068710
Tel: (949) 679-1190 www.zymoresearch.com
Quick-cfDNA™ Serum & Plasma KitHighlights
• High-quality DNA, including cell-free, is easily and robustly purifi ed from up to 10 ml of serum/plasma or up to 1 ml amniotic fl uid or cerebrospinal fl uid.
• Zymo-Spin™ Column technology enables elution of DNA in as little as 35 µl and ensures it is ready for all sensitive downstream applications such as qPCR and Next-Generation sequencing.
Product Cat. No. Size
Quick-cfDNA™ Serum & Plasma Kit D4076 50 preps.
Total DNA is effi ciently purifi ed from cell-free biological fl uids with the Quick-cfDNA™ Serum & Plasma kit. Total DNA, including both high and low molecular weight species, purifi ed (duplicates) from human maternal plasma, amniotic fl uid and cerebrospinal fl uid was analyzed by 2200 TapeStation® (Agilent).
The Quick-cfDNA™ Serum & Plasma kit robustly recovers cell-free DNA when compared to Supplier Q. The DNA recovered is linearly proportional to the input volume of sample when compared with Supplier Q. Concentration of the smallest nucleosomal fragment (N1, ~180bp) DNA was determined using the 2100 Bioanalyzer (Agilent).
Ladder healthy serum Maternal plasma Amnio4c fluid
Cerebrospinal Fluid (CSF)
48,500bp 15,000bp 7,000bp 4,000bp 4,000bp 3,000bp 2,500bp 2,000bp 1,500bp 1,200bp 900bp 600bp 400bp 250bp 100bp
Fragmented DN
A
Total DNA
A) Maternal plasma
Amniotic fl uid
Cerebrospinal fl uid La
dder healthy serum Maternal plasma Amnio4c fluid Cerebrospinal Fluid (CSF)
48,500bp 15,000bp 7,000bp 4,000bp 4,000bp 3,000bp 2,500bp 2,000bp 1,500bp 1,200bp 900bp 600bp 400bp 250bp 100bp
Fragmented DN
A
Total DNA
A) 800
700
600
500
400
300
200
100
0
0 1 2 3 4 5
Cel
l fre
e D
NA
yie
ld (p
g/µ
l)
Plasma Volume (mL)
ZymoSupplier Q
Cell-free DNA recovery is directly scalable using the Quick-cfDNA™ Serum & Plasma Kit. Graphs and gel image show the linear recovery of cfDNA from human plasma and serum (healthy female donors), as measured by 2200 Tapestation® (Agilent, in duplicates).
Plasma/Serum
cfD
NA
[ng
/µl]
2
01 5 10 ml
R2=0.99 (36yr F serum)
R2=0.97 (58yr F serum)
R2=0.99 (54yr F plasma)
R2=0.99 (36yr F plasma)
R2=0.97 (58yr F plasma)
36 yr F plasma
[bp]
Reliable & Effi cient Recovery of Cell-free DNA (up to 10 ml)
Compatible with Many Body Fluids Superior Performance
Learn more and view additional Quick-DNA™ Kit formats at www.zymoresearch.de/dna/quick-dna
www.zymoresearch.deTel: +49 (0)761 60068710
Tel: (949) 679-1190 www.zymoresearch.com
Quick-DNA™ Urine KitHighlights
• Purify cellular and/or cell-free DNA easily and reliably from up to 40 ml of urine.
• Uniquely formulated urine conditioning reagent allows stabilization of DNA in urine for up to one month at ambient temperature.
• Zymo-Spin™ Column technology ensures DNA is ready for all sensitive downstream applications including qPCR, DNA-sequencing, arrays, and DNA methylation analysis.
Product Cat. No. Size
Quick-DNA™ Urine Kit D3061 50 preps.
DNA yields increase linearly with increasing volumes of urine from healthy subjects extracted using the Quick-DNA™ Urine Kit. DNA was isolated from 1 ml, 10 ml, 25 ml, and 40 ml urine. DNA concentration was quantifi ed using the Femto™ Human DNA Quantifi cation Kit (Zymo Research, E2005).
900
800
700
600
500
400
300
200
100
0
0 10 20 30 40
DN
A (n
g)
Urine (ml)
Total (Cellular and Cell-free)
Cellular only
Cell-free only
Both cellular and cell-free DNA can be effectively purifi ed from urine using the Quick-DNA™ Urine Kit. 5 ml of urine from a healthy female donor was processed and DNA was eluted in 20 µl fi nal volume. 1 µl of the sample was analyzed using a 2200 TapeStation.
[bp]48,50015,000
7,000
4,000
3,0002,5002,000
1,5001,200
900
600
400
250
100
Cellular DNA
Urine Total DNALadder
Cell-free DNA
Get cellular or cell-free DNA, or both!
Streamlined, Innovative Workfl ow
Learn more and view additional Quick-DNA™ Kit formats at www.zymoresearch.de/dna/quick-dna
Precipitation Proteinase K Digestion DNA Purifi cation
BindWashElute
Spin
DNA
www.zymoresearch.deTel: +49 (0)761 60068710
Highlights
• Streamlined purifi cation of high-quality FFPE tissue DNA that is ideal for PCR, Next-Generation library prep, enzymatic manipulations, etc.
• Size selection technology; recover total DNA >50 bp or >500 bp.
Quick-DNA™ FFPE Kit
$
$
Deparaffi nized Tissue
$Proteinase K Digestion
Ultra-pure DNA Ready for PCR, Sequencing, etc.
SpinWashElute
Zymo-Spin™ IIC
Product Cat. No. Size
Quick-DNA™ FFPE Kit D3067 50 preps.
100bp DNA >500bp 1kb DNA >50 bp
Equivalent amounts of DNA resolved in a 1% agarose/TAE/EtBr gel show binding conditions may be adjusted with the Quick-DNA™ FFPE Kit to selectively isolate DNA >50 bp or >500 bp. 100 bp DNA ladder and 1 kb DNA ladder from Zymo Research.
500 bp ◄
Selectable DNA Size
Improved Recovery from FFPE Samples
Streamlined Workfl ow
Learn more and view additional Quick-DNA™ Kit formats at www.zymoresearch.de/dna/quick-dna
Flourescence History
Flou
resc
ence
(465
-510
)
Cycles
Zymo
Supplier Q
1 2 25 28 31 34 37 40 43
Equivalent amounts of DNA isolated using Zymo and Supplier Q procedures were used for real time PCR analysis. DNA isolated using the Quick-DNA™ FFPE Kit consistently yielded lower Ct values values as depicted by the amplifi cation curves above.
www.zymoresearch.deTel: +49 (0)761 60068710
Quick-DNA™ Environmental Kits
Product Cat. No. Size
Quick-DNA™ Fecal/Soil Microbe Miniprep Kit D6010 50 preps.
Quick-DNA™ Fungal/Bacterial Miniprep Kit D6005 50 preps.
Quick-DNA™ Tissue & Insect Miniprep Kit D6016 50 preps.
Quick-DNA™ Plant/Seed Miniprep Kit D6020 50 preps.
Improved Recovery of High-quality DNA from Environmental Samples
Ultra-pure, Inhibitor-free DNA
Learn more and view additional Quick-DNA™ Kit formats at www.zymoresearch.de/dna/quick-dna
Our environmental purifi cation kits feature unique BashingBead™ technology, allowing high-recovery of DNA from a wide variety of tough-to-lyse environmental samples. These products lead to high-yield and quality DNA suitable for sensitive downstream applications.
Our novel inhibitor removal technology, featured in our fecal/soil microbe and plant/seed kits, contains the components needed for effi cient removal of polyphenolic compounds that can inhibit downstream enzymatic reactions.
Comparison of DNA yields from rat feces using the Quick-DNA™ Fecal/Soil Microbe Kit and kits from suppliers A and B. Equivalent amounts of feces were processed using each kit and then equal volumes of eluted DNA were analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate.
Supplier A Supplier BQuick-DNA™ Fecal/
Soil Microbe Kit
Fungal and bacterial DNA purifi cation. DNA isolated from Saccharomyces cerevisiae (spores) and E. coli using the Quick-DNA™ Fungal/Bacterial Kit is high-quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the Quick-DNA™ Fungal/Bacterial Kit or the kit from Supplier A. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. The size marker is a 1 kb ladder (Zymo Research).
1 kb Yeast Spores E. coli
Zymo Research Supplier A
Yeast Spores E. coli
Quick-DNA™ Fungal/Bacterial Kit
No Am
plification
No Am
plification
No Am
plificationN
o Amplification
C T
10% Eluate
35% Eluate
ZymoBIOMICS Supplier M Supplier P Supplier Q
17.57 17.48 17.63 17.82
0.00
5.00
10.00
15.00
20.00
25.00
30.00
35.00
40.00
The Quick-DNA™ Environmental Kits provide inhibitor-free DNA even when challenged with extremely inhibitor-rich samples.
Quick-DNA™
www.zymoresearch.deTel: +49 (0)761 60068710
Innovation. Pure & Simple.™
Since its inception in 1994, Zymo Research has been proudly serving the scientific community by providing innovative, reliable, and high quality research tools at affordable prices. Our vision... “The Beauty of Science is to Make Things Simple” is now truer than ever. Whether it’s epigenetics, DNA, RNA, E. coli, or yeast based research, our philosophy remains the same: To provide the highest quality products in the industry while ensuring they are both simple to use and reliable in their performance.
Zymo Research stands on three pillars which form the foundation of our company: Innovation, Quality, and Customer Service. These pillars are fundamental to our culture and ensure our products meet your needs.
InnovationZymo Research is historically recognized for its innovation of high quality nucleic acid purification technologies. Under the branding DNA Purification Made Simple® and RNA Purification Made Simple®, our technologies are pushing the limits of what is possible with nucleic acid isolation. As The Epigenetics Company™, Zymo Research has also received much attention for its rapidly expanding portfolio of epigenetics products and services. It is our objective to develop and provide the most comprehensive set of tools for DNA, RNA, and epigenetic research and analysis available today. Thousands of peer-reviewed scientific publications from researchers around the world feature our technologies. Through innovation, our scientists have made streamlined DNA methylation detection possible, pioneered the micro-elution column for DNA and RNA purification, developed the simplest and the most sophisticated methods for high-quality plasmid DNA purification, and patented the first RNA purification directly from Trizol® without phase separation among many other leading technologies in the industry.
QualityWe are committed to quality and guarantee that all of our products and service will meet and exceed your expectations. Our products are constantly evaluated by scientists like you to help ensure their reliability and the highest standard of quality.
Customer ServiceWe strive for excellence in how we support your scientific endeavors. We pledge to be honest and responsible for everything we do with you. We will treat you as we would like to be treated. Together, we will build a brighter future.
Tel: +49 (0)761 60068710 www.zymoresearch.com
Cat. No. B1001 Version 01