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Enhance the Capacity for Species Identification and Genetic Analysis on Marine Organisms in
the Coral Reef Ecosystems in the Western Pacific (DRMREEF) - PHILIPPINES
PROJECT UPDATE
Ephrime B. Metillo Project Leader-PHILIPPINES
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PHILIPPINES DNA BARCODING:
2014 review: very few (barcoded marine
species
Biodiversity = societal needs
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Phase 1: 200 Species submitted, 96 species with DNA barcodes
DEPOSITED in Mindanao State University - Iligan Institute of Technology
Natural History Museum
Phase 2: 211 Species submitted without DNA barcodes
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1 2 2
2
2
2
Iligan City
Iligan Bay
Bohol Sea
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PRISM: Premier Research Institute of Science and Mathematics
Marine Biodiversity Research Laboratory
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PCR Machine (MJ Reasearch PTC200 Model
Vortex Centrifuge
Pipettors 1 whole set plus stand
PCR Products microcentrifuge set (with
angle rotor PCR tubes) ...
Refrigerated centrifuge
Reagents: QIAGEN DNEasy Tissue Extraction Kit
(supplies)
DNA bands UV transilluminator (Accuris E3000)
Centrifuge tubes and tips (supplies)
MSU-IIT IOC-WESTPAC
DRMREEF2
Sampling Funds
Other lab equipment
Mupid gel electrophoresis machine
Fume hood
MARINE BIODIVERSITY: DNA BARCORDING LABORATORY
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DRMREEF 2 Fish 45 82 211
DRMREEF 1 Fish 48 162 192
Family Species Specimens Group
DRMREEF 1 Lobster 1 1 1
DRMREEF 1 Corals 2 7 7
LUTJANIDAE SCARIDAE
MULLIIDAE SERRANIDAE
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HOPES:
- Proposal submitted to Philippine
government approved
- Smooth running of the DNA barcoding lab
- Biodiversity research appreciated by the
public and politician
- International funding support for reef
recruitment studies (techno-demo site)
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THANKS TO:
• UNESCO IOC-WESTPAC
• MSU-IIT Administration
• People
FOR SUPPORT AND COOPERATION