1. Hossain M.N et al. Bioluminescent Ratiometric Indicator for Analysis of Water Hardness in Household Water. Sensors, 20(11), 3164, 2020 2. Inagaki S, et al. Imaging local brain activity of multiple freely moving mice sharing the same environment. Scientific Reports, 9, 7460, 2019 3. Suzuki K, et al. Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence

Ca2+ microscopy. BMC Research Notes, 11(1),313,2018 4. Arai Y, et al. A spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging. ACS Chemical Biology, 13

(8), 1938-1943, 2018 5. Riani YD, Matsuda T, Takemoto K, Nagai T. Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and

cell ablation. BMC Biology 16, 1, 2018. 6. Kushida Y, Arai Y, SHimono K, Nagai T. Biomimetic chemical sensing by fluorescence signals using a virus-like particle-based platform. ACS

Sensors, 3, 87-92, 2018 7. Maeshima K, Matsuda T, Shindo Y, Imamura H, Tamura S, Imai R, Kawakami S, Nagashima R, Soga T, Noji H, Oka K, Nagai T. Atransient rise

in free Mg2+ ions released from ATP-Mg by its hydrolysis contributes to mitotic chromosome condensation. Current Biology, 28, 444-451, 2018 8. Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T., Acid-tolerant monomeric GFP from Olindias formosa., Cell Chemical Biology,

25, 330-338, 2018 9. Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels M, Okamura Y, Matsuda T, Nagai T. Genetically encoded

bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Scientific Reports, 7, 42398, 201710. Suzuki K, Kimura T, Shinoda H, Bai G, Daniels M, Arai Y, Nakano M, and Nagai T.: Five color variants of bright luminescent protein for real-time

multicolor bioimaging. Nature Communications, 7, 13718, 201611. Tiwari DK, et al. Fast positively-photoswitchable fluorescent protein for ultra-low laser power RESOLFT nanoscopy. Nature Methods, 12, 515-518, 2015.

１）蛍光および発光蛋白質を用いたセンサー開発２）発光センサーを利用した化合物スクリーニングシステムの開発

３）オンサイト生理活性分子計測技術の開発

BiomolecularScience and Engineering

教　授 永井　健治 Professor Takeharu NAGAI 06-6879-8480 ng1@sanken.osaka-u.ac.jp准教授 松田　知己 Associate Professor Tomoki MATSUDA 06-6879-8481 tmatsuda@sanken.osaka-u.ac.jp助　教 服部　　満 Assistant Professor Mitsuru HATTORI 06-6879-8481 8tr@sanken.osaka-u.ac.jp助　教 長部　謙二 Assistant Professor Kenji OSABE 06-6879-8481 o3b@sanken.osaka-u.ac.jp特任教授 宮崎　純一 Specially Appointed Prof. Jun-ichi MIYAZAKI 06-6105-5934 jimiyaza@nutri.med.osaka-u.ac.jp特任准教授 和沢　鉄一 Specially Appointed Assoc. Prof. Tetsuichi WAZAWA 06-6879-8481 wz8@sanken.osaka-u.ac.jp特任助教 圓谷　徹之 Specially Appointed Assis. Prof. Tetsuyuki ENTANI 06-6879-8481 te2@sanken.osaka-u.ac.jp

教　授　永井　健治

図２ 化学発光プローブを用いたオンサイト生理活性分子計測の概念図

図１ 化学発光プローブによるマウス個体を用いた分子活性評価の概念図

37