Download - Fermenter-Design
![Page 1: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/1.jpg)
FERMENTER DESIGN FERMENTER DESIGN
BY:
NIKHIL.K.POTDUKHE M.PHARM
![Page 2: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/2.jpg)
INTRODUCTIONINTRODUCTIONFermenterGuidelines for fermenter designRequirements of a fermenterParameters for fermenter designGas transferHeat transferNutrient transferAeration & agitationReferences
![Page 3: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/3.jpg)
FERMENTERFERMENTER Fermenter provide a suitable environment in which an
organism can efficiently produce a target product, that might be:
1.Cell biomass
2.Metabolite
3.Bioconversion product
The sizes of bioreactor can vary over several orders of magnitudes.
1.The microbial cell (few mm³)
2.Shake flask (100-1000ml)
3.Laboratory fermenter (1-50 L)
4.Pilot scale (0.3-10 m³)
5.Plant scale (2-500 m³)
![Page 4: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/4.jpg)
PARTS OF FERMENTER PARTS OF FERMENTER
11..VesselVessel2.Impellers2.Impellers3.Baffle3.Baffle4.Sparger4.Sparger5.Drain point5.Drain point6.Shaft 6.Shaft 7.Aseptic inoculation pipe7.Aseptic inoculation pipe8.Sampling point8.Sampling point
![Page 5: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/5.jpg)
GUIDELINES FOR FERMENTER DESIGN AND OPERATION Material: Stainless steel Height to diameter ratio of the vessel: 2 to 1 or 3 to 1 Impeller Two or three disk turbine impellers Diameter: 0.3 to 0.4 of tank diameter Agitation speed: 50 – 200 rpm Impeller shaft enters either from the top or bottom. Baffle Four equally spaced to prevent vortex formation Width: one tenth of the tank diameter Sparger Ring sparger (Single orifice for a small Fermenter) Heating or cooling coil For sterilization or to control the temperature
![Page 6: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/6.jpg)
Cont……….
-Agitation and aeration
-Cell suspension
-Enhance the aeration (oxygen limitation problem)
-Mixing
-Problem with shear-sensitive cells
-Heating and cooling
-Sensors
-pH Control
![Page 7: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/7.jpg)
DESIGN OF A FERMENTER Factors to consider when designing a fermenter
Aseptic and regulator capability, long-term reliability
Adequate aeration and agitation
Low power consumption
Temperature and pH controls
Sampling facilities
![Page 8: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/8.jpg)
Cont………..
Large volume & low value products
High value & low volume products
Productivity & yield
Product purification
Water management
Energy requirements
Waste treatment
![Page 9: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/9.jpg)
REQUIREMENTS OF A REQUIREMENTS OF A FERMENTORFERMENTOR
The vessel must be strong enough to withstand pressure
The vessel should not corrupt the fermentation product
Prevention of growth of contaminating microorganism must be provided
Efficient O2 Supply if fermentation is aerobic
![Page 10: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/10.jpg)
Cont……..
Addition of anti-foaming agent as demanded by the
foaming state of the medium
Fermenter should posses temperature Control
Fermenter should posses a mechanism for detecting pH values of culture media
There must be drain in the bottom of the fermenter
![Page 11: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/11.jpg)
MIXING RELATED DESIGN MIXING RELATED DESIGN ISSUESISSUES
• Agitator selection.
• Power draw and torque calculations.
• Scale-up.
• Mechanical design.
• Blending performance (scale of agitation, turnovers-per-minute, blend
time, homogeneity).
• Heat removal, temperature field, possible heat damage.
• Solid-liquid mixing (just-suspended speed, settled solids fraction, cloud
height).
![Page 12: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/12.jpg)
Cont………..
• Reaction performance (productivity, selectivity).
• Surface motion, solids and gas drawdown.
• Shear rates and impact velocities, possible shear damage.
• Optimum feed locations.
• Substrate concentration field, nutrient starvation.
• Oxygen starvation or poisoning (local or global).
• CO2 or other product poisoning (local or global).
• pH control.
• Gas-liquid mixing (mass transfer, gas holdup, power factors).
![Page 13: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/13.jpg)
PARAMETERS FOR THE PARAMETERS FOR THE FERMENTER DESIGNFERMENTER DESIGN
Physical Parameters
Chemical Parameters
Biochemical Parameters
Biological Parameters
![Page 14: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/14.jpg)
PHYSICAL PARAMETERSPHYSICAL PARAMETERS
1. Agitation power & speed
2. Broth volume
3. Color
4. Density
5. Foaming
6. Gas flow rate & humidity
7. Heat generation rate & transfer rate
8. Liquid flow rate
9. Temperature
10. Osmotic pressure
![Page 15: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/15.jpg)
CHEMICAL PARAMETERSCHEMICAL PARAMETERS
1. Amino acid
2. CO2
3. Cat ion level
4. Conductivity
5. Ionic strength
6. Malliard reaction products
7. Nitrogen
8. Nutrient composition
9. O2
10. Phosphorous
![Page 16: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/16.jpg)
BIOCHEMICAL PARAMETERSBIOCHEMICAL PARAMETERS
Amino acidsATP/ADP/AMPCarbohydrates Cell mass compositionEnzyme NAD/NADHVitamins & nucleic acid
![Page 17: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/17.jpg)
BIOLOGICAL PARAMETERSBIOLOGICAL PARAMETERS
Age distributionAggregation & contaminationGenetic instability MutationTotal cell countDegeneration Doubling time
![Page 18: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/18.jpg)
GAS TRANSFERGAS TRANSFER
The theory of gas transfer refers to a process where
the gaseous form of a compound is eventually
dissolved into or driven out of the water .The rate
at which the gas is transferred from air to water or
vice versa is proportional to the area of the gas-
liquid interface and the difference between
saturation concentration and the actual
concentration in the water. These dictate both the
direction and rate of gas transfer at the gas-water
interface.
![Page 19: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/19.jpg)
The availability of the oxygen to the biological system depends upon:
1.Solubility
2.Mass transfer rate of oxygen in the fermentation broth
3.Rate of utilization of DO by microbial biomass
![Page 20: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/20.jpg)
Solubility decreases as temperature or salinity increases. For example, the saturation level of dissolved oxygen is lower in warm-water than coldwater systems.
Gas solubility increases as the total pressure (sum of atmospheric and hydrostatic pressure) or mole fraction increases. In other words, the saturation level increases as the total pressure of the system increases.
The rate of dissolution of a gas into water is proportional to: • The difference between actual and saturation concentrations of the gas in solution, Cs – C • The area of the gas-liquid interface, a(m²) • The thickness of the liquid fi lm, d • The diffusion coefficient, D
dC/dt = KL . a . (Cs – C)
Where:dC/dt = rate of gas transferKL . a = overall mass transfer coefficient
![Page 21: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/21.jpg)
DETERMINATION OF OXYGEN DETERMINATION OF OXYGEN TRANSFER COEFFICIENTSTRANSFER COEFFICIENTS
KLa =no2/(C* – CL )-------1.)
Sodium sulfite oxidation method
Dynamic method
Direct method
Oxygen yield coefficient method
![Page 22: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/22.jpg)
SODIUM SULFITE OXIDATION SODIUM SULFITE OXIDATION METHODMETHOD
This method employs the oxidation of sodium sulfite by oxygen in the presence of copper or cobalt which act as catalyst.
NaSO3 + ½ O2 → Na2SO4
To find KLa by this method, air is sparged through a 1 N Na2SO3
solution in the presence of copper ions at a conc. Of 10¯³ M & mechanical agitation.
The conc. Of dissolved oxygen in the liquid is nearly zero since the oxidation reaction is extremely fast. Therefore,
KLa = no2/C*
Where,
no2 =rate of oxygen transfer
C*=saturation dissolved oxygen conc. in solution
![Page 23: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/23.jpg)
DYNAMIC METHODDYNAMIC METHOD
This method is the simplest one since it requires only the dynamic oxygen balance in a batch culture, which has the following form
dCL/dt = KL . a . (C* – CL)-Qo2X
Where
Qo2X=rate of oxygen consumption per unit mass of cell
![Page 24: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/24.jpg)
This method contains three versions:
Addition of lethal agentThe gassing out methodDynamic oxygen balanceFrequency response technique
Therefore, KLa can be calculated as:
KLa = Qo2X/ C* – CL
![Page 25: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/25.jpg)
DIRECT METHODDIRECT METHOD
This method based upon oxygen balance in inlet & outlet gas streams around a fermenter. oxygen balance for the sparged air yields
no2.T = 1/VL(no2.i ‒no2.o)Where
no2.T = rate of oxygen transfer
VL=volume of fermentation broth
The dissolved & saturated oxygen conc., CL & C*, need to be measured experimentally in order to determine KLa
C* can be calculated from the partial pressure of oxygen in the exit gas stream in small scale fermenter.
![Page 26: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/26.jpg)
Cont…….
But in large scale fermenter, the assumption of a perfectly mixed gas stream may not be valid. So, the
log. mean of the driving force i.e.,(C* – CL)log.mean, between the inlet & outlet of the fermenter should be used in the following equation determining KLa,
KLa = no2.T/ (C* – CL)log.mean
![Page 27: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/27.jpg)
OXYGEN YIELD COEFFICIENT OXYGEN YIELD COEFFICIENT METHODMETHOD
The rate of oxygen transfer can be related to the growth rate of microorganism using the Oxygen yield coefficient according to the following eq.
no2.T = uX/Yo2
Where
U = specific growth rate of microorganism
X = cell conc.
Yo2 = yield coefficient of oxygen
no2.T = oxygen transfer rate
Once no2.T is determined by this equation then equation
1.) can be used to calculate the KLa provided that the dissolved oxygen conc. is measured.
![Page 28: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/28.jpg)
HEAT TRANSFERHEAT TRANSFER
Efficient heat transfer is important in controlling the temperature during sterilization operations
Heat generated in the fermentor needs to be dissipated by cooling
Optimum temp. should be maintain in the fermentor
This can be achieved by:
◦ External jackets◦ Internal coils◦ External surface heat exchanger
![Page 29: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/29.jpg)
TRANSPORT OF NUTRIENTTRANSPORT OF NUTRIENT
Transport of reactants to & from phase to the biological component has a significant impact on the performance of the reactor.
It is affected if the rate of the transport of the limiting nutrients is slower than the rate of utilization by the cells.
![Page 30: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/30.jpg)
AERATION AND AGITATIONThe primary purpose of aeration is to provide
microorganism in submerged culture with sufficient oxygen for metabolic requirements.
Agitation should ensure that a uniform suspension of microbial cells is achieved in a homogenous nutrient medium.
The structural components are:
-- Impellers
-- Baffles
-- Spargers
-- Stirrer gland
![Page 31: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/31.jpg)
IMPELLERSIMPELLERS
Impeller types can be radial, mixed flow, axial and peripheral and are selected on the basis of the pump design and the application.
The number of vanes will affect the efficiency, in
general more vanes are more efficient..
The agitator is required to achieve a no. of mixing objectives ex..bulk fluid & gas phase mixing, oxygen transfer, heat transfer, air dispersion & maintaining a uniform environment through out the vessel contents.
![Page 32: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/32.jpg)
![Page 33: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/33.jpg)
![Page 34: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/34.jpg)
![Page 35: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/35.jpg)
SPARGERSPARGERWhat is a Sparger?Sparger is a technical term for injecting gas into a liquid
or for spraying a liquid onto a solid. Spargers are porous disc or tube assemblies that are also referred to as Bubblers, Carbonators, Aerators, Porous Stones and Diffusers. Porous Metal Spargers are widely used in Gas-Liquid contacting applications that affect everyday living. A common example of sparging can be seen in an aquarium where air is bubbled into the tank through a fine porous “stone” in order to maintain the level of the dissolved oxygen in the water..
![Page 36: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/36.jpg)
Cont…….
Types of spargers:
Porous sparger Orifice Nozzle Combined sparger- agitator
Typical sparger installation
![Page 37: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/37.jpg)
![Page 38: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/38.jpg)
BAFFLES BAFFLES Four baffles are normally incorporated into vessel of all
sizes to prevent a vortex & to improve aeration efficiency.
The agitation increased with wider baffles but drops sharply with narrow baffles.
![Page 39: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/39.jpg)
STIRRER GLANDSTIRRER GLANDThe satisfactory sealing of the stirrer shaft assembly has
been one of the most difficult problems to overcome in the construction of fermentation equipment which can be operated aseptically for long periods.
Types of stirrer glands:
Stuffing boxSimple bush sealMechanical sealMagnetic drive
![Page 40: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/40.jpg)
REFERENCESREFERENCES Principles of fermentation technology, 2nd
edition by P.F.STANBURY & A.WHITAKER
Bioprocess engineering, basic concepts, 2nd edition by M.L.SHULER & F.KARGI
ebook.com
www.google.com
![Page 41: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/41.jpg)
ANY QUERIESANY QUERIES::
![Page 42: Fermenter-Design](https://reader036.vdocument.in/reader036/viewer/2022081416/55721216497959fc0b900539/html5/thumbnails/42.jpg)