Fish Heath Management Lab 5: Histology (microanatomy)
Tarah JohnsonMarch 2nd, 2009
Objectives
• Be able to microscopically identify cells and structures in the liver, kidney, and gills
• Have the opportunity to microscopically examine histological sections of other fish organs (eyes, head, muscle, etc.)
Liver
• Hepatocytes• Hepatic Sinusoid• Central Vein and or Hepatic Portal Vein• Bile Duct• Arteriole• Glycogen Vacuole
Central Vein and Hepatic Sinusoids
Erythrocytes visible within the central vein
Hepatic sinusoids
400x
Bile Duct Arteriole
A. Glycogen Vacuole
A
400x
Erythrocytes visible within arteriole
400x
Head Kidney
• Interrenal gland• Chromaffin cell• Postcardinal vein• Hematopoietic tissue
A
B
C
A. Interrenal Gland
B. Chromaffin Cell
C. Postcardinal vein
400x
A. Postcardinal vein
B. Hematopoietic tissue
40x
A B
Trunk Kidney
• Nephron (may not observe a complete nephron): Renal corpuscle, proximal tube, distal tubule, and collecting duct
• Renal Corpuscle:Glomerulus, Bowman’s capsule, basement membrane of glomerulus
• Melanocytes• Collecting duct and/or Mesonephric duct• Connective tissue
AB
C
A. Renal Corpuscle
B. Proximal Tubule
C. Distal Tubule
400x
400xA. Glomerulus
B. Basement membrane of glomerulus
C. Bowman’s capsule
A
C
B
A
B
A. Mesonephric Duct
B. Melanocytes
C. Connective tissue
400x
C
Gills
• Filament• Lamella• Goblet (or mucous) cell• Chloride cell• Pillar (or pilaster) cell• Gill filament cartilage
Gill filaments and lamellae
Lamella
A
A. Filament cartilage
B. Pillar cell
C. Chloride cell
B
40x
400x
C
Mucous or Goblet Cells
400x
Laboratory Steps
• Use proper microscope handling procedures (distributed in Lab 2)
• Examine histology slides of liver, kidney, and gills
• Examine additional histology slides of other organs
Be Sure to…
• Clean oil immersion lenses• Clean slides and return to proper case (the
same one you took it from)• Turn off microscopes and leave on low power
objective when done
