Gut-Brain Regeneration:The microbiome as a new avenue for
therapeutic management
Zach Bush, MDInternal Medicine, Endocrinology and Metabolism
Founder, Director of Clinical AffairsRevolution Health Center
Scottsville, VA
Founder, CEOBiomic Sciences
Disclosure• I discovered carbon-based bacterial metabolites that
have the potential to function as Redox signaling molecules
• I am founder and CEO of Biomic Sciences that has brought to market RESTORE, the first Carbon-based redox molecule supplement, and my team has created a new paradigm of cell signaling science
• I am the founder and President of Revolution Health Center
• My clinical and basic science operations and experience that I share with you today produce all of my income
USA Ranks 49th in health outcomes
1st World Epidemics• Autism 1:68• Attention Deficit 1:10 (70% medicated)• Asthma 1:10• Allergy 1:4• Diabetes 1:4• Obesity 1:3• Major Depression 1:2• Cancer 1:2• Dementia 1:1
1st World Epidemics• Autism 1:68• Attention Deficit 1:10 (70% medicated)• Asthma 1:10• Allergy 1:4• Diabetes 1:4• Obesity 1:3• Major Depression 1:2• Cancer 1:2• Dementia 1:1
1st World Epidemics• Autism 1:68• Attention Deficit 1:10 (70% medicated)• Asthma 1:10• Allergy 1:4• Diabetes 1:4• Obesity 1:3• Major Depression 1:2• Cancer 1:2• Dementia 1:1
Root Cause of disease?
Root Cause of Aging and Disease
Inflammation is the root of all chronic disorder and disease
Autism
Oxidative Stress
• Acute inflammation can save our lives – Injury Repair– Infection control
• Chronic inflammation– oxidative stress = positive charge/acid– Shutdown/overwhelm of the antioxidant system
chronic inflammation = loss of communication
What is theRoot Cause of Inflammation?
Root Cause of Aging and Disease
Ecosystem News
• Bacterial population patterns in the human gut predict occurrence of chronic inflammatory disease
– Obesity/Diabetes– Asthma– Autoimmunity– Cancers– Dementia– Autism
The GALT
• Gastrointesinal Associated Lymphatic Tissue
GALT: 80% of the antibodies in your body are made here
Gut InflammationFood SensitivityAsthma/EczemaAllergyAutoimmune
Intercellular Tight JunctionsFirewalls of Defense
Gut/Brain Axis
• Gut injury: Gluten, Glyphosate, Lipopolysaccharides
• Zonulin goes systemic• Tight junction injury
– Gut– Blood/brain barrier– Vascular system– Kidney tubules
Gut/Brain Axis
• Leaky Brain– Interleukin inflammatory cytokines– Neuro transmitter dysregulation– Neuro-hormonal stress
A catastrophic vulnerability
Bacteria
UnprotectedMembranes
Tight Junction Toxins
Bacteria
Bacterial metabolites:Carbon-based Redox system
• Each bacterial species (30,000 species) creates a unique subset of carbon metabolites during the digestive process
• These function as an interspecies molecular communication network that allows for coordinated cell protection, injury response, and repair
Carbon Redox
www.restore4life.com
What are your bacteria saying?
• Dysbiosis: broken ecosystem– Monoculture Antibiotics, pesticides, chemicals, processed foods
• Weeds grow up: E.Coli, Klebsiela, Psudomonas, Candida
Probiotics – 3 - 24 species, $30 Billion monoculture
Monoculture = limited vocabulary = limited defense
• Optimal bowel ecology = 20,000 species
Biodiversity = fluid communication = strong defenes
GLUTENToxic Peptides
CXCR3
InflammationZONULIN
ENZ
TJ breakdown
Leaky Gut/Brain Leak GutGLYPHOSATE
BACTERIAL/YEASTOVERGROWTH
Enzymes
Chronic Inflammation
Leaky Gut
Vagal Nerve Stimulation and CNS inflammation
Functional Gut Barrier
www.restore4life.com
Leaky Gut in real time
VEH PT-GliadinCONTROL Gluten in 1 slice of pizza
www.restore4life.com
Bacterial Communication Frontlines of Defense
TIGHT JUNCTIONS
Bacterial communication impacts tight junction function
TE
ER
ohm
s/cm
2
www.restore4life.com
From Sieve to FirewallDouble-click to add an object
VEH PT-GliadinCONTROL GLUTEN
GLUTEN and RESTORE RESTORE ww
w.restore4life.com
Bacterial communication induces differential
Mitochondrial ROS ActivityR
OS
pro
duct
ion
www.restore4life.com
ROS assay RT- PCR
1 3 5 10 60
Minutes
DAMAGED
(MCF-7)
HEALTHY
Bacterial communication impacts Stem Cell Activation
Urin
e C
D13
3
www.restore4life.com
Urine CD133
1st and 2nd birth of our children
HUMAN70 Trillion Cells 25,000 Genes
BACTERIA 20,000 Species
1.4 Quadrillion Cells 2 Million Genes
Bacteria
www.restore4life.com
Epigenetics / Micro RNA
• Which genes turn on/off is determined by your environment
• Micro RNA are non-coding sequences in the genome that turn on and off gene pathways at the DNA level
• 15% of the micro RNA that is circulating in your blood stream today are from the bacteria in your gut– Bacterial genome is turning on and off the human
genes
www.restore4life.com
USA Ranks 49th in health outcomes
www.restore4life.com
The natural history of neurologic injury
• Children are being born into an ever more narrow bacterial ecosystem (vulnerability)
• Breast milk and food/drink thereafter is contaminated with glyphosate-based herbicides
• Gluten, polyunsaturated oils, and other refined foods
(leaky gut/leaky brain occurs)• Exposure to antibiotics, NSAIDS, Alcohol, invasive bacteria,
parasites• Leaky Gut/Leaky Gut Brain – Toxins accumulate
(accelerating neurologic injury)• Widespread immune dysfunction (invasive bacteria, chronic
viremia – HPV, HSV, HepC, HIV)• Shutdown of the antioxidant system (chronic inflammation)• System overwhelm (Autism to Alzheimers, CVD to Cancer)
Leaky Gut - Leaky BrainInflammation cascade from birth to
death Colic
Sensory processing defects Attention Deficit Anxiety Disorders Major Depression
Disordered Sleep Insulin resistance Infertility Diabetes Vascular Disease Cancer Dementia
Gut/Brain Injury What can we do about it?• Tight Junction firewall up and running• Direct toxin antidote – glyphosate, gluten• Promote eco-diversity in the gut and beyond• Increase bacterial breakdown of toxins• Increase bio-availabilty of food nutrients
Maximize the Biome: Get out in nature – breathe biodiversity Grow your own food Eat from the bottom of the food chain Stop Dairy (high glyphosate, highly acidic) Fermented foods:
Miso Soup, Sauerkraut, etc
www.restore4life.com
Soil/Gut Communication Supplementation:
What’s in Restore?• Carbon-based redox molecules extracted from fossil soil
in the South West United States• Trace micronutrients – minerals and amino acids with
concentration profiles similar to that of a sweet potato skin
• Unprecedented results in cellular safety studies– Gold-standard Renal Tubule Toxicity studies – No LD50, extends
life of cell lines at every concentration– Even at 100% concentrations
Restore Logistics• 1 tsp to 1 TBL three times daily before or with
meals• For very sensitive kids:
– 2-5 drops by mouth – plain or
with non-chlorinated water/juice– Slowly increase to 1 tsp three times daily
• Once on restore you can stop probiotics and digestive enzymes to speed recovery of natural gut biome diversity and healthy digestive processes
• No drug-drug interactions
References1. Centers for Disease Control and Prevention (CDC), Key Findings: Trends in the
Parent-Report of Health Care Provider-Diagnosis and Medication Treatment for ADHD: United States, 2003-2011 the National Survey of Children's Health (NSCH)
2. Ellwood P, Williams H, Aït-Khaled N, Björkstén B, Robertson C, ISAAC Phase III Study Group. Translation of questions: The International Study of Asthma and Allergies in Childhood (ISAAC) experience. Int J Tuberc Lung Dis. September 2009; 13(9): 1174-1182
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4. Botman SL, Moore TF, Moriarity CL, Parsons VL. Design and estimation for the National Health Interview Survey, 1995–2004. National Center for Health Statistics. Vital Health Stat 2000;2(130)
5. Centers for Disease Control and Prevention. National Diabetes Statistics Report: Estimates of Diabetes and Its Burden in the United States, 2014. Atlanta, GA: US Department of Health and Human Services; 2014
References6. Janeway, CA Jr.; et al. (2001). "The mucosal immune system". Immunobiology. New
York: Garland Science. 10-13
7. Salminen S, Bouley C, Boutron-Ruault MC, et al. (1998). "Functional food science and gastrointestinal physiology and function". British Journal of Nutrition 80 (S1): S147–S171
8. Moriame G; et al. Viral Suppression and Immune Restoration in the Gastrointestinal Mucosa of HIV Type 1-Infected Patients Initiating Therapy during Primary or Chronic Infection Journal of Virology, August 2006, p. 8236-8247, Vol. 80, No. 16
9. Abbas A.B.; Lichtman A.H. (2009). "Ch.2 Innate Immunity". In Saunders (Elsevier). Basic Immunology. Functions and disorders of the immune system (3rd ed.)
10. Eming, S. A.; Krieg, T.; Davidson, J. M. (2007). "Inflammation in wound repair: molecular and cellular mechanisms". Journal of Investigative Dermatology 127 (3): 514–525
11. Van Dorpe, S; et al. (2012). "Brainpeps: The blood–brain barrier peptide database". Brain structure & function 217 (3): 687–718
12. Schneider, Stefan W.; et al. (March 2004). "Glioblastoma cells release factors that disrupt blood–brain barrier features". Acta Neuropathologica 107 (3): 272–276
References13. H Chen; EE Konofagou. Journal of Cerebral Blood Flow &
Metabolism (2014) 34, 1197–1204
14. H Matsui ; et al. (2011). The pathophysiology of non-steroidal anti-inflammatory drug (NSAID)-induced mucosal injuries in stomach and small intestine J Clin Biochem Nutr. 2011 Mar; 48(2): 107–111
15. Eadon MT; et al. Endotoxemia alters tight junction gene and protein expression in the kidney (2012). Am J Physiol Renal Physiol. 2012 Sep 15; 303(6): F821–F830