Institut of plant biochemistry
L‘interaction non-hôte Persil-Phytophthora sojae, un modèle pour étudier la réponse immunitaire innée chez les plantes
TSGPTS
Open reading frame
RACE clones
cDNA clone
Elicitor activity
+-+--+---
polypeptidesexpressed fromsubclones
Elicitor-activeoligopeptide
TS
C X
X
P H
H N
C C
VWNQPVRGFKVYE
Pep-13, a minimal elicitor motif within GP42
Institute of Plant Biochemistry
Proteine ProteineGln C S + NH 2R
Cys
OGln C NH
R
O
Enzym e
Enzym e
SH
Cys
Protein Protein Gln C S
Cys
OGln C
NH 2
O SH
Cys
Enzym eEnzym e
NH 3
+
Catalytic m ode of action of transglutam inases
R = Am ines, Lysine
Institut of plant biochemistry
GP42 TGase homologs are highly conserved among the genus Phytophthora
Institute of Plant Biochemistry
IPBInstitute of Plant Biochemistry
Pep-13-containing GP42 TGase homologs are highly conserved among Phytophthora spp.
Hydropathy plot of Phytophthora sojae transglutaminasePep-13 is part of a surface-exposed loop structure
IPBInstitute of Plant Biochemistry
TGase and elicitor activities of P. sojae wild-type and mutant proteinsPep-13 is functionally indispensable
IPBInstitute of Plant Biochemistry
Pep-13 elicits defense reactions in potato with the same signal specificity as in parsley
P234A W231A
Institute of Plant Biochemistry